UniProtKB - O66766 (GATB_AQUAE)
Protein
Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B
Gene
gatB
Organism
Aquifex aeolicus (strain VF5)
Status
Functioni
Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction takes place in the presence of glutamine and ATP through an activated phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA(Gln) (By similarity).By similarity
Catalytic activityi
- ATP + H2O + L-glutamine + L-glutamyl-tRNAGln = ADP + H+ + L-glutamate + L-glutaminyl-tRNAGln + phosphate
- ATP + H2O + L-aspartyl-tRNAAsn + L-glutamine = ADP + 2 H+ + L-asparaginyl-tRNAAsn + L-glutamate + phosphate
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity Source: GO_Central
GO - Biological processi
- glutaminyl-tRNAGln biosynthesis via transamidation Source: GO_Central
- translation Source: UniProtKB-UniRule
Keywordsi
Molecular function | Ligase |
Biological process | Protein biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | AAEO224324:G1G15-343-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B (EC:6.3.5.-)Short name: Asp/Glu-ADT subunit B |
Gene namesi | Name:gatB Ordered Locus Names:aq_461 |
Organismi | Aquifex aeolicus (strain VF5) |
Taxonomic identifieri | 224324 [NCBI] |
Taxonomic lineagei | Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000148757 | 1 – 478 | Aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit BAdd BLAST | 478 |
Interactioni
Subunit structurei
Heterotrimer of A, B and C subunits.
By similarityProtein-protein interaction databases
STRINGi | 224324.aq_461 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O66766 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O66766 |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0064, Bacteria |
HOGENOMi | CLU_019240_0_0_0 |
InParanoidi | O66766 |
OMAi | ARKWWMG |
OrthoDBi | 497127at2 |
Family and domain databases
Gene3Di | 1.10.10.410, 1 hit 1.10.150.380, 1 hit |
HAMAPi | MF_00121, GatB, 1 hit |
InterProi | View protein in InterPro IPR004413, Apn/Gln-ADT_bsu IPR017959, Asn/Gln-tRNA_amidoTrfase_suB/E IPR006075, Asn/Gln-tRNA_Trfase_suB/E_cat IPR018027, Asn/Gln_amidotransferase IPR003789, Asn/Gln_tRNA_amidoTrase-B-like IPR042114, GatB_C_1 IPR023168, GatB_Yqey_C_2 IPR017958, Gln-tRNA_amidoTrfase_suB_CS IPR014746, Gln_synth/guanido_kin_cat_dom |
PANTHERi | PTHR11659, PTHR11659, 1 hit |
Pfami | View protein in Pfam PF02934, GatB_N, 1 hit PF02637, GatB_Yqey, 1 hit |
SMARTi | View protein in SMART SM00845, GatB_Yqey, 1 hit |
SUPFAMi | SSF55931, SSF55931, 1 hit SSF89095, SSF89095, 1 hit |
TIGRFAMsi | TIGR00133, gatB, 1 hit |
PROSITEi | View protein in PROSITE PS01234, GATB, 1 hit |
i Sequence
Sequence statusi: Complete.
O66766-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNEKYEAVIG LEIHVQMDTK TKMFCGCKVE FGAEPNTNVC PVCLGMPGAL
60 70 80 90 100
PIVNKRAVEY AIRASLALNC EVHEESVFAR KHYFYPDLPK GYQISQYEKP
110 120 130 140 150
LATNGWVELN LPNGEKKKVR IRRLHIEEDA GKNIHEGDKT LVDLNRAGTP
160 170 180 190 200
LMEIVTEPDI RTPEEARLFL EKLRNIMRYA GVSKADMEKG QLRCDINVSI
210 220 230 240 250
RPKGSKEFGT RVEIKNVNSF RFVQKALEYE IERQINVVEE GGEVVQETRT
260 270 280 290 300
FDPQTGKTYP MRTKEEAEDY RYFPDPDLVP LKVKKEWIEE IKKNMPELPD
310 320 330 340 350
QRFERLIKEY GLSEYEAGIL VNHKEVGDFF EEAVRHFKEP KGIVNWLIND
360 370 380 390 400
LLGLLRDKGI SIEESPVKPE HLAELVKLIK EKVISTKIGK EVIKEMVETG
410 420 430 440 450
KTPSQIVEEK GLKQITDENQ IKELVKKIFE KHPKEVERLK QGEEKLIGFF
460 470
VGQVMRETRG KANPQVVNKV IRELVKEV
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000657 Genomic DNA Translation: AAC06727.1 |
PIRi | B70342 |
RefSeqi | NP_213326.1, NC_000918.1 WP_010880264.1, NC_000918.1 |
Genome annotation databases
EnsemblBacteriai | AAC06727; AAC06727; aq_461 |
KEGGi | aae:aq_461 |
PATRICi | fig|224324.8.peg.382 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000657 Genomic DNA Translation: AAC06727.1 |
PIRi | B70342 |
RefSeqi | NP_213326.1, NC_000918.1 WP_010880264.1, NC_000918.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3H0L | X-ray | 2.30 | B/E/H/K/N/Q/T/W | 1-478 | [»] | |
3H0M | X-ray | 2.80 | B/E/H/K/N/Q/T/W | 1-478 | [»] | |
3H0R | X-ray | 3.00 | B/E/H/K/N/Q/T/W | 1-478 | [»] | |
SMRi | O66766 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224324.aq_461 |
Genome annotation databases
EnsemblBacteriai | AAC06727; AAC06727; aq_461 |
KEGGi | aae:aq_461 |
PATRICi | fig|224324.8.peg.382 |
Phylogenomic databases
eggNOGi | COG0064, Bacteria |
HOGENOMi | CLU_019240_0_0_0 |
InParanoidi | O66766 |
OMAi | ARKWWMG |
OrthoDBi | 497127at2 |
Enzyme and pathway databases
BioCyci | AAEO224324:G1G15-343-MONOMER |
Miscellaneous databases
EvolutionaryTracei | O66766 |
Family and domain databases
Gene3Di | 1.10.10.410, 1 hit 1.10.150.380, 1 hit |
HAMAPi | MF_00121, GatB, 1 hit |
InterProi | View protein in InterPro IPR004413, Apn/Gln-ADT_bsu IPR017959, Asn/Gln-tRNA_amidoTrfase_suB/E IPR006075, Asn/Gln-tRNA_Trfase_suB/E_cat IPR018027, Asn/Gln_amidotransferase IPR003789, Asn/Gln_tRNA_amidoTrase-B-like IPR042114, GatB_C_1 IPR023168, GatB_Yqey_C_2 IPR017958, Gln-tRNA_amidoTrfase_suB_CS IPR014746, Gln_synth/guanido_kin_cat_dom |
PANTHERi | PTHR11659, PTHR11659, 1 hit |
Pfami | View protein in Pfam PF02934, GatB_N, 1 hit PF02637, GatB_Yqey, 1 hit |
SMARTi | View protein in SMART SM00845, GatB_Yqey, 1 hit |
SUPFAMi | SSF55931, SSF55931, 1 hit SSF89095, SSF89095, 1 hit |
TIGRFAMsi | TIGR00133, gatB, 1 hit |
PROSITEi | View protein in PROSITE PS01234, GATB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GATB_AQUAE | |
Accessioni | O66766Primary (citable) accession number: O66766 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | August 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families