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Entry version 124 (02 Jun 2021)
Sequence version 1 (01 Aug 1998)
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Protein

A-adding tRNA nucleotidyltransferase

Gene

aq_411

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

tRNA nucleotidyltransferase involved in the synthesis of the tRNA CCA terminus. Adds the terminal adenosine residue to tRNA (PubMed:11701927, PubMed:25914059).

Can incorporate CMP into tRNA ending with C74C75 (tRNACC), with very weak efficiency (PubMed:25914059).

2 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section provides information relevant to cofactors. A cofactor is any non-protein substance required for a protein to be catalytically active. Some cofactors are inorganic, such as the metal atoms zinc, iron, and copper in various oxidation states. Others, such as most vitamins, are organic.<p><a href='/help/cofactor' target='_top'>More...</a></p>Cofactori

Mg2+1 Publication

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

kcat is 0.10 min(-1) for incorporation of AMP into tRNACC. kcat is 0.00015 min(-1) for incorporation of CMP into tRNACC.1 Publication
  1. KM=98 µM for ATP1 Publication
  2. KM=220 µM for CTP1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section indicates at which position the protein binds a given metal ion. The nature of the metal is indicated in the 'Description' field.<p><a href='/help/metal' target='_top'>More...</a></p>Metal bindingi472Magnesium1 Publication1
    Metal bindingi474Magnesium1 Publication1
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei550ATP; via carbonyl oxygen1 Publication1
    Binding sitei603ATP1 Publication1
    Binding sitei632ATP1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi459 – 462ATP1 Publication1 Publication4
    Nucleotide bindingi545 – 546ATP1 Publication1 Publication2
    Nucleotide bindingi590 – 599ATP1 Publication10

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionNucleotidyltransferase, RNA-binding, Transferase, tRNA-binding
    Biological processtRNA processing
    LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    AAEO224324:G1G15-304-MONOMER

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    A-adding tRNA nucleotidyltransferaseCurated (EC:2.7.7.-1 Publication)
    Short name:
    A-adding TNTCurated
    Alternative name(s):
    A-adding enzyme1 Publication
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Ordered Locus Names:aq_411Imported
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiAquifex aeolicus (strain VF5)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224324 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000000798 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00004475631 – 824A-adding tRNA nucleotidyltransferaseAdd BLAST824

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    224324.aq_411

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    Secondary structure

    1824
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    O66728

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    Protein Data Bank in Europe - Knowledge Base

    More...
    PDBe-KBi
    Search...

    Miscellaneous databases

    Relative evolutionary importance of amino acids within a protein sequence

    More...
    EvolutionaryTracei
    O66728

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini305 – 363CBS 1PROSITE-ProRule annotationAdd BLAST59
    Domaini367 – 423CBS 2PROSITE-ProRule annotationAdd BLAST57

    <p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

    Adopts a seahorse-like shape and consists of four domains: head, neck, body, and tail.1 Publication1 Publication

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Keywords - Domaini

    CBS domain, Repeat

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    COG0517, Bacteria
    COG0617, Bacteria
    COG0618, Bacteria

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_015961_5_0_0

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    O66728

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    VMALGIH

    Database of Orthologous Groups

    More...
    OrthoDBi
    2018439at2

    Family and domain databases

    Conserved Domains Database

    More...
    CDDi
    cd05398, NT_ClassII-CCAase, 1 hit

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    3.30.460.10, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR000644, CBS_dom
    IPR001667, DDH_dom
    IPR038763, DHH_sf
    IPR003156, DHHA1_dom
    IPR043519, NT_sf
    IPR002646, PolA_pol_head_dom

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF00571, CBS, 2 hits
    PF01368, DHH, 1 hit
    PF02272, DHHA1, 1 hit
    PF01743, PolyA_pol, 1 hit

    Simple Modular Architecture Research Tool; a protein domain database

    More...
    SMARTi
    View protein in SMART
    SM00116, CBS, 2 hits

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF64182, SSF64182, 1 hit
    SSF81301, SSF81301, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS51371, CBS, 2 hits

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    O66728-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MVCPKVVILS EGADLDSLSA AYGVLKLYPD AYLLKPKHLS KKAGEVFKKY
    60 70 80 90 100
    RDKFRVIEDL PDCFELVLVD THFLPEGLPR ERIKRIIVYD HHPIGDVKEF
    110 120 130 140 150
    EGKIEKVGAA TTLVVEEIKE KGIDINPRDA TLLAFGIYED TGNFTYEGTT
    160 170 180 190 200
    PRDALALAFL LEKGANLREI REVVMETYTP EQIEAVGKIV QSIEKVFING
    210 220 230 240 250
    RQISFATAVL ERYQPDINTL LYEIKDLKES DAFFVIIEAE GKTYVFGRSQ
    260 270 280 290 300
    SEDVDVGEIL SHFGGGGHRE AGAVKLENVS AERIKELIKA FLKRKYVKLK
    310 320 330 340 350
    VRDIMNTPPF VLEEHVSVKD ALTELSERGI ANAPVINREG KLVGIISKKA
    360 370 380 390 400
    LLKLVKLYPD EPIELFVNRD FYTLSPDAPV WEAEEILTKF GQKLIPVVED
    410 420 430 440 450
    GTVVGVVTRL DILQAVKEDL EKLKEKRRKI KVPENIEEIA REVGQIAKEM
    460 470 480 490 500
    GLRAYIVGGV VRDILLGKEV WDVDFVVEGN AIELAKELAR RHGVNVHPFP
    510 520 530 540 550
    EFGTAHLKIG KLKLEFATAR RETYPRPGAY PKVEPASLKE DLIRRDFTIN
    560 570 580 590 600
    AMAISVNLED YGTLIDYFGG LRDLKDKVIR VLHPVSFIED PVRILRALRF
    610 620 630 640 650
    AGRLNFKLSR STEKLLKQAV NLGLLKEAPR GRLINEIKLA LREDRFLEIL
    660 670 680 690 700
    ELYRKYRVLE EIIEGFQWNE KVLQKLYALR KVVDWHALEF SEERIDYGWL
    710 720 730 740 750
    YLLILISNLD YERGKHFLEE MSAPSWVRET YKFMKFKLGS LKEELKKAKE
    760 770 780 790 800
    NYEVYRLLKP LHTSVLLLLM LEEELKEKIK LYLEKLRKVK LPKEKIEELK
    810 820
    KQGLKGKELG ERIEELKREI MNKI
    Length:824
    Mass (Da):94,658
    Last modified:August 1, 1998 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iA5B627695C2DAF73
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AE000657 Genomic DNA Translation: AAC06692.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    D70337

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_213288.1, NC_000918.1
    WP_010880226.1, NC_000918.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    AAC06692; AAC06692; aq_411

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    aae:aq_411

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|224324.8.peg.338

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000657 Genomic DNA Translation: AAC06692.1
    PIRiD70337
    RefSeqiNP_213288.1, NC_000918.1
    WP_010880226.1, NC_000918.1

    3D structure databases

    Select the link destinations:

    Protein Data Bank Europe

    More...
    PDBei

    Protein Data Bank RCSB

    More...
    RCSB PDBi

    Protein Data Bank Japan

    More...
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1VFGX-ray2.80A/B443-824[»]
    4WBYX-ray1.50A428-824[»]
    4WBZX-ray3.30A/B443-824[»]
    4WC0X-ray3.10A/B443-824[»]
    4WC1X-ray3.10A/B443-824[»]
    4WC2X-ray2.80A443-824[»]
    4WC3X-ray3.10A443-824[»]
    4WC4X-ray3.50A443-824[»]
    4WC5X-ray3.41A443-824[»]
    4WC6X-ray3.41A443-824[»]
    4WC7X-ray3.10A443-824[»]
    4X0AX-ray3.50A443-824[»]
    4X0BX-ray3.20A443-824[»]
    SMRiO66728
    ModBaseiSearch...
    PDBe-KBiSearch...

    Protein-protein interaction databases

    STRINGi224324.aq_411

    Genome annotation databases

    EnsemblBacteriaiAAC06692; AAC06692; aq_411
    KEGGiaae:aq_411
    PATRICifig|224324.8.peg.338

    Phylogenomic databases

    eggNOGiCOG0517, Bacteria
    COG0617, Bacteria
    COG0618, Bacteria
    HOGENOMiCLU_015961_5_0_0
    InParanoidiO66728
    OMAiVMALGIH
    OrthoDBi2018439at2

    Enzyme and pathway databases

    BioCyciAAEO224324:G1G15-304-MONOMER

    Miscellaneous databases

    EvolutionaryTraceiO66728

    Family and domain databases

    CDDicd05398, NT_ClassII-CCAase, 1 hit
    Gene3Di3.30.460.10, 1 hit
    InterProiView protein in InterPro
    IPR000644, CBS_dom
    IPR001667, DDH_dom
    IPR038763, DHH_sf
    IPR003156, DHHA1_dom
    IPR043519, NT_sf
    IPR002646, PolA_pol_head_dom
    PfamiView protein in Pfam
    PF00571, CBS, 2 hits
    PF01368, DHH, 1 hit
    PF02272, DHHA1, 1 hit
    PF01743, PolyA_pol, 1 hit
    SMARTiView protein in SMART
    SM00116, CBS, 2 hits
    SUPFAMiSSF64182, SSF64182, 1 hit
    SSF81301, SSF81301, 1 hit
    PROSITEiView protein in PROSITE
    PS51371, CBS, 2 hits

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiAATNT_AQUAE
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O66728
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 3, 2019
    Last sequence update: August 1, 1998
    Last modified: June 2, 2021
    This is version 124 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    3D-structure, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
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