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Entry version 123 (10 Feb 2021)
Sequence version 1 (01 Aug 1998)
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Protein

Proline--tRNA ligase

Gene

proS

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the 'Function' section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

  1. KM=0.06 mM for proline (at 60 degrees Celsius)1 Publication
  2. KM=0.05 mM for cysteine (at 60 degrees Celsius)1 Publication

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    GO - Biological processi

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionAminoacyl-tRNA synthetase, Ligase
    Biological processProtein biosynthesis
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    AAEO224324:G1G15-266-MONOMER

    SABIO-RK: Biochemical Reaction Kinetics Database

    More...
    SABIO-RKi
    O66690

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Proline--tRNA ligase (EC:6.1.1.15)
    Alternative name(s):
    Prolyl-tRNA synthetase
    Short name:
    ProRS
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:proS
    Ordered Locus Names:aq_365
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiAquifex aeolicus (strain VF5)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri224324 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
    <p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000000798 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    Keywords - Cellular componenti

    Cytoplasm

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002482261 – 570Proline--tRNA ligaseAdd BLAST570

    Proteomic databases

    PRoteomics IDEntifications database

    More...
    PRIDEi
    O66690

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    <p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

    Homodimer.

    By similarity

    Protein-protein interaction databases

    STRING: functional protein association networks

    More...
    STRINGi
    224324.aq_365

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    O66690

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

    Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain.By similarity

    <p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    COG0442, Bacteria

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    CLU_016739_0_0_0

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    O66690

    Identification of Orthologs from Complete Genome Data

    More...
    OMAi
    QESGRWD

    Database of Orthologous Groups

    More...
    OrthoDBi
    665824at2

    Family and domain databases

    Conserved Domains Database

    More...
    CDDi
    cd00861, ProRS_anticodon_short, 1 hit
    cd00779, ProRS_core_prok, 1 hit

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    3.40.50.800, 1 hit
    3.90.960.10, 1 hit

    HAMAP database of protein families

    More...
    HAMAPi
    MF_01569, Pro_tRNA_synth_type1, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR002314, aa-tRNA-synt_IIb
    IPR006195, aa-tRNA-synth_II
    IPR004154, Anticodon-bd
    IPR036621, Anticodon-bd_dom_sf
    IPR002316, Pro-tRNA-ligase_IIa
    IPR004500, Pro-tRNA-synth_IIa_bac-type
    IPR023717, Pro-tRNA-Synthase_IIa_type1
    IPR044140, ProRS_anticodon_short
    IPR033730, ProRS_core_prok
    IPR036754, YbaK/aa-tRNA-synt-asso_dom_sf
    IPR007214, YbaK/aa-tRNA-synth-assoc-dom

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF03129, HGTP_anticodon, 1 hit
    PF00587, tRNA-synt_2b, 1 hit
    PF04073, tRNA_edit, 1 hit

    Protein Motif fingerprint database; a protein domain database

    More...
    PRINTSi
    PR01046, TRNASYNTHPRO

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF55826, SSF55826, 1 hit

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR00409, proS_fam_II, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS50862, AA_TRNA_LIGASE_II, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    O66690-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MRWSRYFLYT EKEEPKEAEA PSHRLLLKAG FIKQVSAGIY ELLPPAYKVL
    60 70 80 90 100
    KKVESIIRKE MDRSGAQELL LTVLNPKELW EETGRWETYG EELFKLKDRN
    110 120 130 140 150
    GREYCLGPTH EEEITDLVRR VVRSYRQLPV ILYQIQVKFR DEKRPRFGLI
    160 170 180 190 200
    RAREFIMKDA YSFDTDDMSA MISYEAMKFA YQRIFNKLRL NVIMAEADVG
    210 220 230 240 250
    QIGGKMSHEF IAFTDYGEAK VAYCENCGYA ANAEIVPLPK PEEEKEEEKP
    260 270 280 290 300
    MEKVHTPNVH TIEELSKFLD VHPSKIMKAV LYIVNEKEPV LVLIRGDREI
    310 320 330 340 350
    DENKLEKVLG TDNFRLATDE EVQELLGTKK GFIGIFNLPE NIKVLWDNSL
    360 370 380 390 400
    YGVKNLVVAL NEPDWHYINV NPGRDFQYGE FVDVAEVREG DPCPKCGSPL
    410 420 430 440 450
    KVRRGLELGH IFLLGTRYSE PMKAYFTDRD GKEKPIIMGC YGIGVSRILA
    460 470 480 490 500
    ALVEQYHDDK GIKWPTPVAP FELDIILLNT KDEEMKNVAE KLYLEAEEKG
    510 520 530 540 550
    IDVIFDDREE SPGFKFADAD LVGFPYRIVV GKKVKEGKVE VQSRHTGEKW
    560 570
    DVEIEKAIDF VKEKIEEDKK
    Length:570
    Mass (Da):65,873
    Last modified:August 1, 1998 - v1
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i6B12F0FA016DAE14
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    AE000657 Genomic DNA Translation: AAC06648.1

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    F70332

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_213250.1, NC_000918.1
    WP_010880188.1, NC_000918.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    AAC06648; AAC06648; aq_365

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    aae:aq_365

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|224324.8.peg.295

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AE000657 Genomic DNA Translation: AAC06648.1
    PIRiF70332
    RefSeqiNP_213250.1, NC_000918.1
    WP_010880188.1, NC_000918.1

    3D structure databases

    SMRiO66690
    ModBaseiSearch...

    Protein-protein interaction databases

    STRINGi224324.aq_365

    Proteomic databases

    PRIDEiO66690

    Genome annotation databases

    EnsemblBacteriaiAAC06648; AAC06648; aq_365
    KEGGiaae:aq_365
    PATRICifig|224324.8.peg.295

    Phylogenomic databases

    eggNOGiCOG0442, Bacteria
    HOGENOMiCLU_016739_0_0_0
    InParanoidiO66690
    OMAiQESGRWD
    OrthoDBi665824at2

    Enzyme and pathway databases

    BioCyciAAEO224324:G1G15-266-MONOMER
    SABIO-RKiO66690

    Family and domain databases

    CDDicd00861, ProRS_anticodon_short, 1 hit
    cd00779, ProRS_core_prok, 1 hit
    Gene3Di3.40.50.800, 1 hit
    3.90.960.10, 1 hit
    HAMAPiMF_01569, Pro_tRNA_synth_type1, 1 hit
    InterProiView protein in InterPro
    IPR002314, aa-tRNA-synt_IIb
    IPR006195, aa-tRNA-synth_II
    IPR004154, Anticodon-bd
    IPR036621, Anticodon-bd_dom_sf
    IPR002316, Pro-tRNA-ligase_IIa
    IPR004500, Pro-tRNA-synth_IIa_bac-type
    IPR023717, Pro-tRNA-Synthase_IIa_type1
    IPR044140, ProRS_anticodon_short
    IPR033730, ProRS_core_prok
    IPR036754, YbaK/aa-tRNA-synt-asso_dom_sf
    IPR007214, YbaK/aa-tRNA-synth-assoc-dom
    PfamiView protein in Pfam
    PF03129, HGTP_anticodon, 1 hit
    PF00587, tRNA-synt_2b, 1 hit
    PF04073, tRNA_edit, 1 hit
    PRINTSiPR01046, TRNASYNTHPRO
    SUPFAMiSSF55826, SSF55826, 1 hit
    TIGRFAMsiTIGR00409, proS_fam_II, 1 hit
    PROSITEiView protein in PROSITE
    PS50862, AA_TRNA_LIGASE_II, 1 hit

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiSYP_AQUAE
    <p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O66690
    <p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
    Last sequence update: August 1, 1998
    Last modified: February 10, 2021
    This is version 123 of the entry and version 1 of the sequence. See complete history.
    <p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families
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