UniProtKB - O66663 (KDTA_AQUAE)
Protein
3-deoxy-D-manno-octulosonic acid transferase
Gene
kdtA
Organism
Aquifex aeolicus (strain VF5)
Status
Functioni
Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes the transfer of a single 3-deoxy-D-manno-octulosonate (Kdo) residue from CMP-Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate precursor of lipid A. Is strictly monofunctional, i.e. is capable of adding only a single Kdo residue to the acceptor lipid.1 Publication
Catalytic activityi
- CMP-3-deoxy-β-D-manno-octulosonate + lipid IVA (E. coli) = α-Kdo-(2→6)-lipid IVA + CMP + H+2 PublicationsEC:2.4.99.122 Publications
Temperature dependencei
Highly thermostable. Does not lose activity after 16 hours at 70 or 80 degrees Celsius, but incubation of the enzyme at 90 and 99 degrees Celsius for 1 hour results in gradual loss of activity.1 Publication
: LPS core biosynthesis Pathwayi
This protein is involved in the pathway LPS core biosynthesis, which is part of Bacterial outer membrane biogenesis.View all proteins of this organism that are known to be involved in the pathway LPS core biosynthesis and in Bacterial outer membrane biogenesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 31 | Proton acceptor1 Publication | 1 | |
Sitei | 98 | Transition state stabilizer | 1 | |
Sitei | 162 | Transition state stabilizer | 1 |
GO - Molecular functioni
- Kdo transferase activity Source: UniProtKB-EC
- transferase activity Source: GO_Central
GO - Biological processi
- lipid A biosynthetic process Source: GO_Central
- lipopolysaccharide core region biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Transferase |
Biological process | Lipopolysaccharide biosynthesis |
Enzyme and pathway databases
BioCyci | AAEO224324:G1G15-237-MONOMER |
BRENDAi | 2.4.99.12, 396 |
UniPathwayi | UPA00958 |
Protein family/group databases
CAZyi | GT30, Glycosyltransferase Family 30 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-deoxy-D-manno-octulosonic acid transferase (EC:2.4.99.122 Publications)Short name: Kdo transferase Alternative name(s): Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase Monofunctional Kdo transferase |
Gene namesi | Name:kdtA Ordered Locus Names:aq_326 |
Organismi | Aquifex aeolicus (strain VF5) |
Taxonomic identifieri | 224324 [NCBI] |
Taxonomic lineagei | Bacteria › Aquificae › Aquificales › Aquificaceae › Aquifex › |
Proteomesi |
|
Subcellular locationi
- Cell inner membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
GO - Cellular componenti
- plasma membrane Source: GO_Central
Keywords - Cellular componenti
Cell inner membrane, Cell membrane, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 30 | G → A: Complete loss of catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 31 | E → A: Large decrease in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 98 | E → A: Nearly complete loss of catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 162 | K → A: Large decrease in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 212 | R → A: Decrease in catalytic activity. 1 Publication | 1 | |
Mutagenesisi | 276 | E → A: Decrease in catalytic activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000419171 | 1 – 353 | 3-deoxy-D-manno-octulosonic acid transferaseAdd BLAST | 353 |
Interactioni
Subunit structurei
Can form homodimer, homotrimer and homotetramer.
2 PublicationsProtein-protein interaction databases
STRINGi | 224324.aq_326 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O66663 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O66663 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 211 – 212 | CMP-Kdo binding | 2 | |
Regioni | 247 – 249 | CMP-Kdo binding | 3 | |
Regioni | 273 – 276 | CMP-Kdo binding | 4 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG1519, Bacteria |
HOGENOMi | CLU_036146_2_0_0 |
InParanoidi | O66663 |
OMAi | MEAELWY |
OrthoDBi | 1163086at2 |
Family and domain databases
Gene3Di | 3.40.50.11720, 1 hit |
InterProi | View protein in InterPro IPR007507, Glycos_transf_N IPR038107, Glycos_transf_N_sf IPR039901, Kdotransferase |
PANTHERi | PTHR42755, PTHR42755, 1 hit |
Pfami | View protein in Pfam PF04413, Glycos_transf_N, 1 hit |
i Sequence
Sequence statusi: Complete.
O66663-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MQFEVLKRFF PKESLKNCKG ALWVHTASIG EFNTFLPILK ELKREHRILL
60 70 80 90 100
TYFSPRAREY LKTKSDFYDC LHPLPLDNPF SVKRFEELSK PKALIVVERE
110 120 130 140 150
FWPSLIIFTK VPKILVNAYA KGSLIEKILS KKFDLIIMRT QEDVEKFKTF
160 170 180 190 200
GAKRVFSCGN LKFICQKGKG IKLKGEFIVA GSIHTGEVEI ILKAFKEIKK
210 220 230 240 250
TYSSLKLILV PRHIENAKIF EKKARDFGFK TSFFENLEGD VILVDRFGIL
260 270 280 290 300
KELYPVGKIA IVGGTFVNIG GHNLLEPTCW GIPVIYGPYT HKVNDLKEFL
310 320 330 340 350
EKEGAGFEVK NETELVTKLT ELLSVKKEIK VEEKSREIKG CYLEKLREFL
RGL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000657 Genomic DNA Translation: AAC06622.1 |
PIRi | C70329 |
RefSeqi | NP_213223.1, NC_000918.1 WP_010880161.1, NC_000918.1 |
Genome annotation databases
EnsemblBacteriai | AAC06622; AAC06622; aq_326 |
KEGGi | aae:aq_326 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE000657 Genomic DNA Translation: AAC06622.1 |
PIRi | C70329 |
RefSeqi | NP_213223.1, NC_000918.1 WP_010880161.1, NC_000918.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2XCI | X-ray | 2.00 | A/B/C/D | 1-353 | [»] | |
2XCU | X-ray | 2.42 | A/B/C/D | 1-353 | [»] | |
SMRi | O66663 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 224324.aq_326 |
Protein family/group databases
CAZyi | GT30, Glycosyltransferase Family 30 |
Genome annotation databases
EnsemblBacteriai | AAC06622; AAC06622; aq_326 |
KEGGi | aae:aq_326 |
Phylogenomic databases
eggNOGi | COG1519, Bacteria |
HOGENOMi | CLU_036146_2_0_0 |
InParanoidi | O66663 |
OMAi | MEAELWY |
OrthoDBi | 1163086at2 |
Enzyme and pathway databases
UniPathwayi | UPA00958 |
BioCyci | AAEO224324:G1G15-237-MONOMER |
BRENDAi | 2.4.99.12, 396 |
Miscellaneous databases
EvolutionaryTracei | O66663 |
Family and domain databases
Gene3Di | 3.40.50.11720, 1 hit |
InterProi | View protein in InterPro IPR007507, Glycos_transf_N IPR038107, Glycos_transf_N_sf IPR039901, Kdotransferase |
PANTHERi | PTHR42755, PTHR42755, 1 hit |
Pfami | View protein in Pfam PF04413, Glycos_transf_N, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KDTA_AQUAE | |
Accessioni | O66663Primary (citable) accession number: O66663 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 3, 2012 |
Last sequence update: | August 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 98 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families