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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Aquifex aeolicus (strain VF5)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22S-adenosyl-L-methionineUniRule annotation1
Binding sitei47S-adenosyl-L-methionineUniRule annotation1
Binding sitei74S-adenosyl-L-methionineUniRule annotation1
Active sitei97By similarity1
Binding sitei97S-adenosyl-L-methionineUniRule annotation1
Binding sitei101SubstrateUniRule annotation1
Binding sitei133SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciAAEO224324:G1G15-54-MONOMER
BRENDAi2.1.1.33 396
UniPathwayi
UPA00989

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:aq_065
OrganismiAquifex aeolicus (strain VF5)
Taxonomic identifieri224324 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesAquificaceaeAquifex
Proteomesi
  • UP000000798 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001712861 – 297tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST297

Interactioni

Protein-protein interaction databases

STRINGi224324.aq_065

Structurei

3D structure databases

ProteinModelPortaliO66479
SMRiO66479
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni165 – 168Substrate bindingUniRule annotation4

Domaini

The C-terminal region is probably involved in protein stability.

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000075620
InParanoidiO66479
KOiK03439
OMAiPWPKAGH
OrthoDBiPOG091H015P

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit

Sequencei

Sequence statusi: Complete.

O66479-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLCYVNYKRV KRPVEIPNLE VEIGFGRGDF IVKLAKENPD KNFFGIEISQ
60 70 80 90 100
ISIEKLMKRV GKKGLKNVYC TNVDAYWGFY FLFRDNYVEN IYMNYPDPWF
110 120 130 140 150
KKRHHKRRLT KPERLYMFAK KLKLGGEIRI RTDNYEFLEF TKESAKVLDC
160 170 180 190 200
FEVEEGTLNV KEPLTKYEQK WLSMGKTLYK LILRKVKEPK FVEHPEVEEV
210 220 230 240 250
RELFPVKVKV ESVDPKKIES REIKLDEEVY FKTFKVWQRD KDFLVECLLS
260 270 280 290
EKGYLQKFFI QIKRKEDGYV IDVSPYSEVL RTRNLQRSIQ TVAQLLS
Length:297
Mass (Da):35,639
Last modified:August 1, 1998 - v1
Checksum:i8CF24B95B3FD1599
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA Translation: AAC06452.1
PIRiD70306
RefSeqiNP_213039.1, NC_000918.1
WP_010879977.1, NC_000918.1

Genome annotation databases

EnsemblBacteriaiAAC06452; AAC06452; aq_065
GeneIDi1192492
KEGGiaae:aq_065
PATRICifig|224324.8.peg.56

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000657 Genomic DNA Translation: AAC06452.1
PIRiD70306
RefSeqiNP_213039.1, NC_000918.1
WP_010879977.1, NC_000918.1

3D structure databases

ProteinModelPortaliO66479
SMRiO66479
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224324.aq_065

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC06452; AAC06452; aq_065
GeneIDi1192492
KEGGiaae:aq_065
PATRICifig|224324.8.peg.56

Phylogenomic databases

eggNOGiENOG4105CZ1 Bacteria
COG0220 LUCA
HOGENOMiHOG000075620
InParanoidiO66479
KOiK03439
OMAiPWPKAGH
OrthoDBiPOG091H015P

Enzyme and pathway databases

UniPathwayi
UPA00989

BioCyciAAEO224324:G1G15-54-MONOMER
BRENDAi2.1.1.33 396

Family and domain databases

HAMAPiMF_01057 tRNA_methyltr_TrmB, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR003358 tRNA_(Gua-N-7)_MeTrfase_Trmb
PANTHERiPTHR23417 PTHR23417, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR00091 TIGR00091, 1 hit
PROSITEiView protein in PROSITE
PS51625 SAM_MT_TRMB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_AQUAE
AccessioniPrimary (citable) accession number: O66479
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: August 1, 1998
Last modified: February 28, 2018
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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