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Protein

Probable pyruvate kinase, cytosolic isozyme

Gene

At4g26390

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key regulatory enzyme of the glycolytic pathway that catalyzes the final step of glycolysis, converting ADP and phosphoenolpyruvate (PEP) to ATP and pyruvate by essentially irreversible transphosphorylation.By similarity

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase GAPC2, cytosolic (GAPC2), Glyceraldehyde-3-phosphate dehydrogenase GAPC1, cytosolic (GAPC1)
  2. Phosphoglycerate kinase 3, cytosolic (PGK3)
  3. Probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 2 (At3g08590), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 (PGM1)
  4. Enolase 1, chloroplastic (ENO1), Cytosolic enolase 3 (ENO3), Bifunctional enolase 2/transcriptional activator (ENO2)
  5. Pyruvate kinase (AXX17_At5g51800), Pyruvate kinase (AXX17_At3g24740), Pyruvate kinase (AXX17_At5g63250), Pyruvate kinase (AXX17_At3g50280), Pyruvate kinase (AXX17_ATUG04870), Pyruvate kinase (MAH20.13), Pyruvate kinase (MCD7.8), Pyruvate kinase (MBK5.16), Pyruvate kinase (F1I16_60), Pyruvate kinase (At3g25960), Pyruvate kinase (AXX17_At3g50440), Plastidial pyruvate kinase 3, chloroplastic (PKP3), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At5g08490), Pyruvate kinase (AXX17_At5g55530), Pyruvate kinase (AXX17_At3g28070), Pyruvate kinase (At2g36580), Pyruvate kinase (AXX17_At3g47400), Pyruvate kinase, Probable pyruvate kinase, cytosolic isozyme (At4g26390), Pyruvate kinase (F8J2_160), Pyruvate kinase (At3g52990), Pyruvate kinase (AXX17_At3g03460), Plastidial pyruvate kinase 2 (PKP2), Pyruvate kinase (AXX17_At2g33300), Pyruvate kinase (At3g04050), Pyruvate kinase (AXX17_At4g30430), Pyruvate kinase (F1I16_220), Plastidial pyruvate kinase 1, chloroplastic (PKP1), Pyruvate kinase (AXX17_At1g33230), Pyruvate kinase (At5g63680), Pyruvate kinase, Plastidial pyruvate kinase 4, chloroplastic (PKP4)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei37SubstrateBy similarity1
Metal bindingi39PotassiumBy similarity1
Metal bindingi41PotassiumBy similarity1
Metal bindingi71PotassiumBy similarity1
Metal bindingi72Potassium; via carbonyl oxygenBy similarity1
Binding sitei227Substrate; via amide nitrogenBy similarity1
Sitei227Transition state stabilizerBy similarity1
Metal bindingi229MagnesiumBy similarity1
Binding sitei252Substrate; via amide nitrogenBy similarity1
Metal bindingi253MagnesiumBy similarity1
Binding sitei253Substrate; via amide nitrogenBy similarity1
Binding sitei285SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BioCyciARA:AT4G26390-MONOMER
ReactomeiR-ATH-6798695 Neutrophil degranulation
R-ATH-70171 Glycolysis
UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pyruvate kinase, cytosolic isozyme (EC:2.7.1.40)
Short name:
PK
Gene namesi
Ordered Locus Names:At4g26390
ORF Names:M3E9.180
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 4

Organism-specific databases

AraportiAT4G26390
TAIRilocus:2131453 AT4G26390

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001121231 – 497Probable pyruvate kinase, cytosolic isozymeAdd BLAST497

Proteomic databases

PaxDbiO65595
PRIDEiO65595

Expressioni

Gene expression databases

ExpressionAtlasiO65595 baseline and differential
GenevisibleiO65595 AT

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi3702.AT4G26390.1

Structurei

3D structure databases

ProteinModelPortaliO65595
SMRiO65595
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiKOG2323 Eukaryota
COG0469 LUCA
HOGENOMiHOG000021559
InParanoidiO65595
KOiK00873
OMAiRIMLHAA
OrthoDBiEOG093607JW
PhylomeDBiO65595

Family and domain databases

CDDicd00288 Pyruvate_Kinase, 1 hit
Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 1 hit
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

O65595-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMEQRPKTK IVCTLGPASR SVPMVEKLLM AGMSVARFNF SHGSYEYHQE
60 70 80 90 100
TLDNLRQAML NTGMLCAVML DTKGPEIRTG FLKDGKPIQL KQGQEITIST
110 120 130 140 150
DYDLKGDEKT ICMSYKKLAQ DVNPGMVILC ADGTISLKVL SCDKEKGTVR
160 170 180 190 200
CRCENTSMLG ERKNVNLPGV VVDLPTLTEK DKQDILEWGV PNQIDMIALS
210 220 230 240 250
FVRKGSDLVQ VRKLLGKHAK TILLMSKVEN QEGVANFDDI LINSDAFMIA
260 270 280 290 300
RGDLGMEIPI EKIFLAQKVM IYKCNFMGKP VVTATQMLES MIKSPRPTRA
310 320 330 340 350
EATDVANAVL DGTDCVMLSG ETAAGAYPEL AVRTMAKICV EAESTLDYGD
360 370 380 390 400
IFKRIMLHAA VPMSPMESLA SSAVRTATSS RATLMMVLTR GGSTARLVAK
410 420 430 440 450
YRPGIPILSV VVPEITSDSF DWACSNEAPA RHSLIYRGLV PVLYAGSARA
460 470 480 490
SIDESTEETL EFASEYGKKK QLCKTGDSVV ALFRTGNAIV IKILTVK
Length:497
Mass (Da):54,319
Last modified:August 1, 1998 - v1
Checksum:i45D43EF54A89FE62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL022223 Genomic DNA Translation: CAA18231.1
AL161565 Genomic DNA Translation: CAB79494.1
CP002687 Genomic DNA Translation: AEE85192.1
PIRiT05065
RefSeqiNP_194369.1, NM_118772.2

Genome annotation databases

EnsemblPlantsiAT4G26390.1; AT4G26390.1; AT4G26390
GeneIDi828745
GrameneiAT4G26390.1; AT4G26390.1; AT4G26390
KEGGiath:AT4G26390

Similar proteinsi

Entry informationi

Entry nameiKPYC_ARATH
AccessioniPrimary (citable) accession number: O65595
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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