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UniProtKB - O65482 (CRK23_ARATH)
Protein
Putative cysteine-rich receptor-like protein kinase 23
Gene
CRK23
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Caution
Possesses one additional DUF26 domain, as compared to other members of the CRK subfamily.Curated
Catalytic activityi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 536 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 633 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 514 – 522 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: GO_Central
- protein serine kinase activity Source: RHEA
GO - Biological processi
- defense response to bacterium Source: GO_Central
- protein phosphorylation Source: GO_Central
Keywordsi
Molecular function | Kinase, Receptor, Serine/threonine-protein kinase, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Putative cysteine-rich receptor-like protein kinase 23 (EC:2.7.11.-)Short name: Cysteine-rich RLK23 |
Gene namesi | Name:CRK23 Ordered Locus Names:At4g23310 ORF Names:F16G20.10, F21P8.200 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G23310 |
TAIRi | locus:2121626, AT4G23310 |
Subcellular locationi
Other locations
- Membrane Curated; Single-pass membrane protein Curated
Plasma Membrane
- plasma membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 24 – 430 | ExtracellularSequence analysisAdd BLAST | 407 | |
Transmembranei | 431 – 451 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 452 – 830 | CytoplasmicSequence analysisAdd BLAST | 379 |
Keywords - Cellular componenti
MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
ChainiPRO_0000295070 | 24 – 830 | Putative cysteine-rich receptor-like protein kinase 23Add BLAST | 807 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 35 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 182 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 216 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 261 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 287 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 298 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 356 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 428 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 581 | PhosphotyrosineBy similarity | 1 | |
Modified residuei | 673 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 681 | PhosphotyrosineBy similarity | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
PaxDbi | O65482 |
ProteomicsDBi | 224525 |
Expressioni
Inductioni
By salicylic acid (SA).1 Publication
Gene expression databases
ExpressionAtlasi | O65482, baseline and differential |
Genevisiblei | O65482, AT |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 27 – 133 | Gnk2-homologous 1PROSITE-ProRule annotationAdd BLAST | 107 | |
Domaini | 140 – 244 | Gnk2-homologous 2PROSITE-ProRule annotationAdd BLAST | 105 | |
Domaini | 250 – 354 | Gnk2-homologous 3PROSITE-ProRule annotationAdd BLAST | 105 | |
Domaini | 508 – 787 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 280 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 363 – 382 | DisorderedSequence analysisAdd BLAST | 20 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 366 – 381 | Pro residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CRK subfamily.PROSITE-ProRule annotation
Keywords - Domaini
Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502QWDY, Eukaryota |
HOGENOMi | CLU_000288_35_2_1 |
InParanoidi | O65482 |
OrthoDBi | 345452at2759 |
PhylomeDBi | O65482 |
Family and domain databases
Gene3Di | 3.30.430.20, 3 hits |
InterProi | View protein in InterPro IPR002902, GNK2 IPR038408, GNK2_sf IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF07714, PK_Tyr_Ser-Thr, 1 hit PF01657, Stress-antifung, 3 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51473, GNK2, 3 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
O65482-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSSWASFIFL FIFSFLTSFR VFAQDPTYRY HSCPNTTIFS RDSAYFSNLQ
60 70 80 90 100
TLLSFLSSPD ASSSYSSGFR NDAVGTFPDR VTGLFDCRGD LPPEVCHNCV
110 120 130 140 150
AFAVKDTLIR CPNERDVTLF YDECTLRYSN LVVTSALDPT YVYHVCPSWA
160 170 180 190 200
TFPRSSTYMT NLITLLSTLS SPSASYSTGF QNATAGKHPD RVTGLFNCRG
210 220 230 240 250
DVSPEVCRRC VSFAVNETST RCPIEKEVTL YYDQCTLRYS NRNILSTSNT
260 270 280 290 300
NGGIILANSQ NMTSNEQARF KDLVLTTMNQ ATIAAANSSK RFDARSANFT
310 320 330 340 350
TLHSLYTLVQ CTHDLTRQDC LSCLQQIINQ LPTEKIGGQF IVPSCSSRFE
360 370 380 390 400
LCLFYNQSAV TTPQPQQNSA PPPPPTSIPS PRPGLNSRFP LITCLSAVSF
410 420 430 440 450
EKFKDLLKPG FICILIKIVS ENTGKGGNSS VIIIAVVVSI TALLLLFVAV
460 470 480 490 500
FSVRTKRRKK MIGAIPLLNV KRKDTEVTEP LAENGDSITT AGSLQFDFKA
510 520 530 540 550
IVAATNNFLP INKLGQGGFG EVYKGTFPSG VQVAVKRLSK TSGQGEREFE
560 570 580 590 600
NEVVVVAKLQ HRNLVRLLGY CLEGEEKILV YEFVHNKSLD YFLFDTTMKR
610 620 630 640 650
QLDWTRRYKI IGGIARGILY LHQDSRLTII HRDLKAGNIL LDADMNPKVA
660 670 680 690 700
DFGMARIFGM DQTEANTRRV VGTYGYMAPE YAMYGQFSMK SDVYSFGVLV
710 720 730 740 750
FEIISGMKNS SLYQMDDSVS NLVTYTWRLW SNGSQLDLVD PSFGDNYQTH
760 770 780 790 800
DITRCIHIAL LCVQEDVDDR PNMSAIVQML TTSSIVLAVP KQPGFFFRGR
810 820 830
HEQVGEVGSS VDRLALCSID DASITSVAPR
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8B6F3 | A0A1P8B6F3_ARATH | Cysteine-rich RLK (RECEPTOR-like pr... | CRK23 At4g23310, F21P8.200, F21P8_200 | 790 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL022347 Genomic DNA Translation: CAA18478.1 AL031326 Genomic DNA Translation: CAA20452.1 AL161559 Genomic DNA Translation: CAB79286.1 CP002687 Genomic DNA Translation: AEE84737.1 |
PIRi | T04848 |
RefSeqi | NP_194062.1, NM_118460.2 |
Genome annotation databases
EnsemblPlantsi | AT4G23310.1; AT4G23310.1; AT4G23310 |
GeneIDi | 828430 |
Gramenei | AT4G23310.1; AT4G23310.1; AT4G23310 |
KEGGi | ath:AT4G23310 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AL022347 Genomic DNA Translation: CAA18478.1 AL031326 Genomic DNA Translation: CAA20452.1 AL161559 Genomic DNA Translation: CAB79286.1 CP002687 Genomic DNA Translation: AEE84737.1 |
PIRi | T04848 |
RefSeqi | NP_194062.1, NM_118460.2 |
3D structure databases
SMRi | O65482 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT4G23310.1 |
Proteomic databases
PaxDbi | O65482 |
ProteomicsDBi | 224525 |
Genome annotation databases
EnsemblPlantsi | AT4G23310.1; AT4G23310.1; AT4G23310 |
GeneIDi | 828430 |
Gramenei | AT4G23310.1; AT4G23310.1; AT4G23310 |
KEGGi | ath:AT4G23310 |
Organism-specific databases
Araporti | AT4G23310 |
TAIRi | locus:2121626, AT4G23310 |
Phylogenomic databases
eggNOGi | ENOG502QWDY, Eukaryota |
HOGENOMi | CLU_000288_35_2_1 |
InParanoidi | O65482 |
OrthoDBi | 345452at2759 |
PhylomeDBi | O65482 |
Miscellaneous databases
PROi | PR:O65482 |
Gene expression databases
ExpressionAtlasi | O65482, baseline and differential |
Genevisiblei | O65482, AT |
Family and domain databases
Gene3Di | 3.30.430.20, 3 hits |
InterProi | View protein in InterPro IPR002902, GNK2 IPR038408, GNK2_sf IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR001245, Ser-Thr/Tyr_kinase_cat_dom IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF07714, PK_Tyr_Ser-Thr, 1 hit PF01657, Stress-antifung, 3 hits |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS51473, GNK2, 3 hits PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CRK23_ARATH | |
Accessioni | O65482Primary (citable) accession number: O65482 Secondary accession number(s): Q7FKK0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 10, 2007 |
Last sequence update: | August 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families