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UniProtKB - O65398 (GLX1_ARATH)
Protein
Lactoylglutathione lyase GLX1
Gene
GLX1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.
By similarityCatalytic activityi
- EC:4.4.1.5By similarity
Cofactori
Zn2+By similarityNote: Binds 1 zinc ion per subunit. In the homodimer, two zinc ions are bound between subunits.By similarity
: methylglyoxal degradation Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes (R)-lactate from methylglyoxal.By similarity This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 20 | Zinc; via tele nitrogenBy similarity | 1 | |
Binding sitei | 24 | SubstrateBy similarity | 1 | |
Metal bindingi | 71 | ZincBy similarity | 1 | |
Binding sitei | 75 | SubstrateBy similarity | 1 | |
Metal bindingi | 89 | Zinc; via tele nitrogenBy similarity | 1 | |
Binding sitei | 89 | SubstrateBy similarity | 1 | |
Active sitei | 137 | Proton donor/acceptorPROSITE-ProRule annotation | 1 | |
Metal bindingi | 137 | ZincBy similarity | 1 | |
Metal bindingi | 150 | Divalent metal cationPROSITE-ProRule annotation | 1 | |
Metal bindingi | 150 | Zinc; shared with dimeric partnerBy similarity | 1 | |
Binding sitei | 150 | Substrate; shared with dimeric partnerBy similarity | 1 | |
Binding sitei | 154 | Substrate; shared with dimeric partnerBy similarity | 1 | |
Metal bindingi | 201 | Divalent metal cationPROSITE-ProRule annotation | 1 | |
Metal bindingi | 201 | Zinc; shared with dimeric partnerBy similarity | 1 | |
Binding sitei | 205 | Substrate; shared with dimeric partnerBy similarity | 1 | |
Metal bindingi | 219 | Divalent metal cationPROSITE-ProRule annotation | 1 | |
Metal bindingi | 271 | Divalent metal cationPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- dioxygenase activity Source: UniProtKB-KW
- lactoylglutathione lyase activity Source: TAIR
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
Keywordsi
Molecular function | Dioxygenase, Lyase, Oxidoreductase |
Ligand | Manganese, Metal-binding, Zinc |
Enzyme and pathway databases
BioCyci | ARA:AT1G11840-MONOMER |
UniPathwayi | UPA00619;UER00675 |
Names & Taxonomyi
Protein namesi | Recommended name: Lactoylglutathione lyase GLX11 Publication (EC:4.4.1.5By similarity)Alternative name(s): Glyoxalase I1 Publication Short name: AtGLX11 Publication Short name: GlyI1 Publication |
Gene namesi | Name:GLX11 Publication Ordered Locus Names:At1g11840Imported ORF Names:F12F1.32Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G11840 |
TAIRi | locus:2008930, AT1G11840 |
Subcellular locationi
Chloroplast
- chloroplast envelope Source: TAIR
Cytosol
- cytosol Source: TAIR
Extracellular region or secreted
- extracellular region Source: TAIR
Peroxisome
- peroxisome Source: TAIR
Vacuole
- plant-type vacuole Source: TAIR
Other locations
- cytoplasm Source: GO_Central
- secretory vesicle Source: TAIR
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000441174 | 2 – 283 | Lactoylglutathione lyase GLX1Add BLAST | 282 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources | 1 |
Post-translational modificationi
Phosphorylated by SnRK2.8.1 Publication
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PRIDEi | O65398 |
ProMEXi | O65398 |
ProteomicsDBi | 247404 [O65398-1] |
PTM databases
iPTMneti | O65398 |
SwissPalmi | O65398 |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
IntActi | O65398, 2 interactors |
STRINGi | 3702.AT1G11840.6 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 17 – 141 | VOC 1PROSITE-ProRule annotationAdd BLAST | 125 | |
Domaini | 147 – 275 | VOC 2PROSITE-ProRule annotationAdd BLAST | 129 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 251 – 252 | Substrate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the glyoxalase I family.Curated
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG2943, Eukaryota |
HOGENOMi | CLU_030607_2_0_1 |
OMAi | MGDAWGH |
OrthoDBi | 1513831at2759 |
PhylomeDBi | O65398 |
Family and domain databases
Gene3Di | 3.10.180.10, 2 hits |
InterProi | View protein in InterPro IPR029068, Glyas_Bleomycin-R_OHBP_Dase IPR004360, Glyas_Fos-R_dOase_dom IPR004361, Glyoxalase_1 IPR018146, Glyoxalase_1_CS IPR037523, VOC |
Pfami | View protein in Pfam PF00903, Glyoxalase, 2 hits |
SUPFAMi | SSF54593, SSF54593, 2 hits |
TIGRFAMsi | TIGR00068, glyox_I, 2 hits |
PROSITEi | View protein in PROSITE PS00934, GLYOXALASE_I_1, 2 hits PS00935, GLYOXALASE_I_2, 1 hit PS51819, VOC, 2 hits |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: O65398-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAEASDLLEW PKKDNRRFLH VVYRVGDLDR TIEFYTEVFG MKLLRKRDIP
60 70 80 90 100
EEKYSNAFLG FGPETSNFVV ELTYNYGVSS YDIGTGFGHF AISTQDVSKL
110 120 130 140 150
VENVRAKGGN VTREPGPVKG GGSVIAFVKD PDGYTFELIQ RGPTPEPFCQ
160 170 180 190 200
VMLRVGDLDR AIKFYEKALG MRLLRKIERP EYKYTIGMMG YAEEYESIVL
210 220 230 240 250
ELTYNYDVTE YTKGNAYAQI AIGTDDVYKS GEVIKIVNQE LGGKITREAG
260 270 280
PLPGLGTKIV SFLDPDGWKT VLVDNKDFLK ELE
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 40 | G → C in BAH19947 (PubMed:19423640).Curated | 1 | |
Sequence conflicti | 45 | R → W in AAL07227 (PubMed:14593172).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_059032 | 1 | M → MNEIASASMLRLCQCFISIC NVHFVSMRAAESSFLLSRNM in isoform 2. | 1 | |
Alternative sequenceiVSP_059033 | 220 – 232 | IAIGT…YKSGE → AQMMCTKAVKLLR in isoform 3. Add BLAST | 13 | |
Alternative sequenceiVSP_059034 | 233 – 283 | Missing in isoform 3. Add BLAST | 51 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC002131 Genomic DNA Translation: AAC17630.1 CP002684 Genomic DNA Translation: AEE28792.1 CP002684 Genomic DNA Translation: AEE28793.1 CP002684 Genomic DNA Translation: AEE28794.1 CP002684 Genomic DNA Translation: AEE28795.1 CP002684 Genomic DNA Translation: AEE28796.1 CP002684 Genomic DNA Translation: AEE28797.1 AB050553 mRNA Translation: BAB17665.1 AF428272 mRNA Translation: AAL16104.1 AY056148 mRNA Translation: AAL07227.1 AY074569 mRNA Translation: AAL67109.1 AY097360 mRNA Translation: AAM19876.1 AK316841 mRNA Translation: BAH19553.1 AK317270 mRNA Translation: BAH19947.1 AK318730 mRNA Translation: BAH56845.1 AY087874 mRNA Translation: AAM65426.1 |
PIRi | F86252 |
RefSeqi | NP_001031025.1, NM_001035948.1 [O65398-1] NP_001031026.1, NM_001035949.3 [O65398-3] NP_001184968.1, NM_001198039.1 [O65398-2] NP_172648.1, NM_101055.4 [O65398-1] NP_849643.1, NM_179312.4 [O65398-1] NP_849644.2, NM_179313.2 [O65398-1] |
Genome annotation databases
EnsemblPlantsi | AT1G11840.1; AT1G11840.1; AT1G11840 [O65398-1] AT1G11840.2; AT1G11840.2; AT1G11840 [O65398-1] AT1G11840.3; AT1G11840.3; AT1G11840 [O65398-1] AT1G11840.4; AT1G11840.4; AT1G11840 [O65398-1] AT1G11840.5; AT1G11840.5; AT1G11840 [O65398-3] AT1G11840.6; AT1G11840.6; AT1G11840 [O65398-2] |
GeneIDi | 837731 |
Gramenei | AT1G11840.1; AT1G11840.1; AT1G11840 [O65398-1] AT1G11840.2; AT1G11840.2; AT1G11840 [O65398-1] AT1G11840.3; AT1G11840.3; AT1G11840 [O65398-1] AT1G11840.4; AT1G11840.4; AT1G11840 [O65398-1] AT1G11840.5; AT1G11840.5; AT1G11840 [O65398-3] AT1G11840.6; AT1G11840.6; AT1G11840 [O65398-2] |
KEGGi | ath:AT1G11840 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC002131 Genomic DNA Translation: AAC17630.1 CP002684 Genomic DNA Translation: AEE28792.1 CP002684 Genomic DNA Translation: AEE28793.1 CP002684 Genomic DNA Translation: AEE28794.1 CP002684 Genomic DNA Translation: AEE28795.1 CP002684 Genomic DNA Translation: AEE28796.1 CP002684 Genomic DNA Translation: AEE28797.1 AB050553 mRNA Translation: BAB17665.1 AF428272 mRNA Translation: AAL16104.1 AY056148 mRNA Translation: AAL07227.1 AY074569 mRNA Translation: AAL67109.1 AY097360 mRNA Translation: AAM19876.1 AK316841 mRNA Translation: BAH19553.1 AK317270 mRNA Translation: BAH19947.1 AK318730 mRNA Translation: BAH56845.1 AY087874 mRNA Translation: AAM65426.1 |
PIRi | F86252 |
RefSeqi | NP_001031025.1, NM_001035948.1 [O65398-1] NP_001031026.1, NM_001035949.3 [O65398-3] NP_001184968.1, NM_001198039.1 [O65398-2] NP_172648.1, NM_101055.4 [O65398-1] NP_849643.1, NM_179312.4 [O65398-1] NP_849644.2, NM_179313.2 [O65398-1] |
3D structure databases
AlphaFoldDBi | O65398 |
SMRi | O65398 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | O65398, 2 interactors |
STRINGi | 3702.AT1G11840.6 |
PTM databases
iPTMneti | O65398 |
SwissPalmi | O65398 |
Proteomic databases
PRIDEi | O65398 |
ProMEXi | O65398 |
ProteomicsDBi | 247404 [O65398-1] |
Genome annotation databases
EnsemblPlantsi | AT1G11840.1; AT1G11840.1; AT1G11840 [O65398-1] AT1G11840.2; AT1G11840.2; AT1G11840 [O65398-1] AT1G11840.3; AT1G11840.3; AT1G11840 [O65398-1] AT1G11840.4; AT1G11840.4; AT1G11840 [O65398-1] AT1G11840.5; AT1G11840.5; AT1G11840 [O65398-3] AT1G11840.6; AT1G11840.6; AT1G11840 [O65398-2] |
GeneIDi | 837731 |
Gramenei | AT1G11840.1; AT1G11840.1; AT1G11840 [O65398-1] AT1G11840.2; AT1G11840.2; AT1G11840 [O65398-1] AT1G11840.3; AT1G11840.3; AT1G11840 [O65398-1] AT1G11840.4; AT1G11840.4; AT1G11840 [O65398-1] AT1G11840.5; AT1G11840.5; AT1G11840 [O65398-3] AT1G11840.6; AT1G11840.6; AT1G11840 [O65398-2] |
KEGGi | ath:AT1G11840 |
Organism-specific databases
Araporti | AT1G11840 |
TAIRi | locus:2008930, AT1G11840 |
Phylogenomic databases
eggNOGi | KOG2943, Eukaryota |
HOGENOMi | CLU_030607_2_0_1 |
OMAi | MGDAWGH |
OrthoDBi | 1513831at2759 |
PhylomeDBi | O65398 |
Enzyme and pathway databases
UniPathwayi | UPA00619;UER00675 |
BioCyci | ARA:AT1G11840-MONOMER |
Miscellaneous databases
PROi | PR:O65398 |
Gene expression databases
ExpressionAtlasi | O65398, baseline and differential |
Family and domain databases
Gene3Di | 3.10.180.10, 2 hits |
InterProi | View protein in InterPro IPR029068, Glyas_Bleomycin-R_OHBP_Dase IPR004360, Glyas_Fos-R_dOase_dom IPR004361, Glyoxalase_1 IPR018146, Glyoxalase_1_CS IPR037523, VOC |
Pfami | View protein in Pfam PF00903, Glyoxalase, 2 hits |
SUPFAMi | SSF54593, SSF54593, 2 hits |
TIGRFAMsi | TIGR00068, glyox_I, 2 hits |
PROSITEi | View protein in PROSITE PS00934, GLYOXALASE_I_1, 2 hits PS00935, GLYOXALASE_I_2, 1 hit PS51819, VOC, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | GLX1_ARATH | |
Accessioni | O65398Primary (citable) accession number: O65398 Secondary accession number(s): B9DFN6 Q940A4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 30, 2017 |
Last sequence update: | August 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 169 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families