UniProtKB - O65202 (ACOX1_ARATH)
Protein
Peroxisomal acyl-coenzyme A oxidase 1
Gene
ACX1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Catalyzes the desaturation of both long- and medium-chain acyl-CoAs to 2-trans-enoyl-CoAs. Most active with C14-CoA. Activity on long-chain mono-unsaturated substrates is 40% higher than with the corresponding saturated substrates. Seems to be an important factor in the general metabolism of root tips. May be involved in the biosynthesis of jasmonic acid.
Catalytic activityi
- EC:1.3.3.6
Cofactori
FADNote: Binds 1 FAD per subunit.
Kineticsi
- KM=5.3 µM for C14-CoA
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 138 | FADBy similarity | 1 | |
Binding sitei | 177 | FAD; via amide nitrogenBy similarity | 1 | |
Active sitei | 424 | Proton acceptorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 399 – 404 | FAD | 6 |
GO - Molecular functioni
- acyl-CoA oxidase activity Source: TAIR
- FAD binding Source: InterPro
- fatty acid binding Source: GO_Central
- flavin adenine dinucleotide binding Source: GO_Central
- palmitoyl-CoA oxidase activity Source: GO_Central
GO - Biological processi
- fatty acid beta-oxidation Source: TAIR
- fatty acid beta-oxidation using acyl-CoA oxidase Source: GO_Central
- jasmonic acid biosynthetic process Source: TAIR
- lipid homeostasis Source: GO_Central
- long-chain fatty acid metabolic process Source: TAIR
- response to cadmium ion Source: TAIR
- response to fungus Source: TAIR
- response to wounding Source: TAIR
- very long-chain fatty acid metabolic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Fatty acid metabolism, Lipid metabolism |
Ligand | FAD, Flavoprotein |
Enzyme and pathway databases
BioCyci | ARA:AT4G16760-MONOMER MetaCyc:AT4G16760-MONOMER |
BRENDAi | 1.3.3.6, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: Peroxisomal acyl-coenzyme A oxidase 1 (EC:1.3.3.6)Short name: AOX 1 Alternative name(s): Long-chain acyl-CoA oxidase Short name: AtCX1 |
Gene namesi | Name:ACX1 Ordered Locus Names:At4g16760 ORF Names:dl4405c, FCAALL.119 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G16760 |
TAIRi | locus:2129121, AT4G16760 |
Subcellular locationi
Peroxisome
- Peroxisome Curated
Cytosol
- cytosol Source: TAIR
Peroxisome
- peroxisome Source: TAIR
Other locations
- plasmodesma Source: TAIR
Keywords - Cellular componenti
PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204689 | 1 – 664 | Peroxisomal acyl-coenzyme A oxidase 1Add BLAST | 664 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 467 ↔ 576 |
Keywords - PTMi
Disulfide bondProteomic databases
PaxDbi | O65202 |
PRIDEi | O65202 |
ProteomicsDBi | 244350 [O65202-1] |
PTM databases
MetOSitei | O65202 |
Expressioni
Tissue specificityi
Expressed mainly in flowers and young seedlings. Lower expression in roots, leaves and bracts.1 Publication
Developmental stagei
Induced by seed imbibition with a peak at day 2 and then declines to reach a basal level 4 days after sowing.1 Publication
Inductioni
Induced by dehydration, abscisic acid (ABA) and jasmonic acid (JA), and localy and systemically by wounding.1 Publication
Gene expression databases
ExpressionAtlasi | O65202, baseline and differential |
Genevisiblei | O65202, AT |
Interactioni
Subunit structurei
Homodimer.
Protein-protein interaction databases
BioGRIDi | 12674, 5 interactors |
IntActi | O65202, 2 interactors |
STRINGi | 3702.AT4G16760.1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O65202 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O65202 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 662 – 664 | Microbody targeting signal | 3 |
Sequence similaritiesi
Belongs to the acyl-CoA oxidase family.Curated
Phylogenomic databases
eggNOGi | KOG0136, Eukaryota |
InParanoidi | O65202 |
OMAi | NQMGHLL |
OrthoDBi | 416859at2759 |
PhylomeDBi | O65202 |
Family and domain databases
Gene3Di | 1.10.540.10, 1 hit |
InterProi | View protein in InterPro IPR029320, Acyl-CoA_ox_N IPR006091, Acyl-CoA_Oxase/DH_cen-dom IPR012258, Acyl-CoA_oxidase IPR002655, Acyl-CoA_oxidase_C IPR036250, AcylCo_DH-like_C IPR037069, AcylCoA_DH/ox_N_sf IPR009100, AcylCoA_DH/oxidase_NM_dom |
Pfami | View protein in Pfam PF01756, ACOX, 1 hit PF02770, Acyl-CoA_dh_M, 1 hit PF14749, Acyl-CoA_ox_N, 1 hit |
PIRSFi | PIRSF000168, Acyl-CoA_oxidase, 1 hit |
SUPFAMi | SSF47203, SSF47203, 2 hits SSF56645, SSF56645, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: O65202-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEGIDHLADE RNKAEFDVED MKIVWAGSRH AFEVSDRIAR LVASDPVFEK
60 70 80 90 100
SNRARLSRKE LFKSTLRKCA HAFKRIIELR LNEEEAGRLR HFIDQPAYVD
110 120 130 140 150
LHWGMFVPAI KGQGTEEQQK KWLSLANKMQ IIGCYAQTEL GHGSNVQGLE
160 170 180 190 200
TTATFDPKTD EFVIHTPTQT ASKWWPGGLG KVSTHAVVYA RLITNGKDYG
210 220 230 240 250
IHGFIVQLRS LEDHSPLPNI TVGDIGTKMG NGAYNSMDNG FLMFDHVRIP
260 270 280 290 300
RDQMLMRLSK VTREGEYVPS DVPKQLVYGT MVYVRQTIVA DASNALSRAV
310 320 330 340 350
CIATRYSAVR RQFGAHNGGI ETQVIDYKTQ QNRLFPLLAS AYAFRFVGEW
360 370 380 390 400
LKWLYTDVTE RLAASDFATL PEAHACTAGL KSLTTTATAD GIEECRKLCG
410 420 430 440 450
GHGYLWCSGL PELFAVYVPA CTYEGDNVVL QLQVARFLMK TVAQLGSGKV
460 470 480 490 500
PVGTTAYMGR AAHLLQCRSG VQKAEDWLNP DVVLEAFEAR ALRMAVTCAK
510 520 530 540 550
NLSKFENQEQ GFQELLADLV EAAIAHCQLI VVSKFIAKLE QDIGGKGVKK
560 570 580 590 600
QLNNLCYIYA LYLLHKHLGD FLSTNCITPK QASLANDQLR SLYTQVRPNA
610 620 630 640 650
VALVDAFNYT DHYLNSVLGR YDGNVYPKLF EEALKDPLND SVVPDGYQEY
660
LRPVLQQQLR TARL
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF4JMK8 | F4JMK8_ARATH | Acyl-coenzyme A oxidase | ACX1 acyl-CoA oxidase 1, ATACX1, At4g16760, Dl4405c, FCAALL.119 | 651 | Annotation score: |
Sequence cautioni
The sequence CAB10450 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB78718 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF057044 mRNA Translation: AAC13498.1 Z97341 Genomic DNA Translation: CAB10450.1 Sequence problems. AL161544 Genomic DNA Translation: CAB78718.1 Sequence problems. CP002687 Genomic DNA Translation: AEE83798.1 AY058849 mRNA Translation: AAL24237.1 BT001067 mRNA Translation: AAN46824.1 |
PIRi | H71434 T52121 |
RefSeqi | NP_567513.4, NM_117778.8 [O65202-1] |
Genome annotation databases
EnsemblPlantsi | AT4G16760.1; AT4G16760.1; AT4G16760 [O65202-1] |
GeneIDi | 827381 |
Gramenei | AT4G16760.1; AT4G16760.1; AT4G16760 [O65202-1] |
KEGGi | ath:AT4G16760 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF057044 mRNA Translation: AAC13498.1 Z97341 Genomic DNA Translation: CAB10450.1 Sequence problems. AL161544 Genomic DNA Translation: CAB78718.1 Sequence problems. CP002687 Genomic DNA Translation: AEE83798.1 AY058849 mRNA Translation: AAL24237.1 BT001067 mRNA Translation: AAN46824.1 |
PIRi | H71434 T52121 |
RefSeqi | NP_567513.4, NM_117778.8 [O65202-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1W07 | X-ray | 2.00 | A/B | 1-659 | [»] | |
SMRi | O65202 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 12674, 5 interactors |
IntActi | O65202, 2 interactors |
STRINGi | 3702.AT4G16760.1 |
PTM databases
MetOSitei | O65202 |
Proteomic databases
PaxDbi | O65202 |
PRIDEi | O65202 |
ProteomicsDBi | 244350 [O65202-1] |
Genome annotation databases
EnsemblPlantsi | AT4G16760.1; AT4G16760.1; AT4G16760 [O65202-1] |
GeneIDi | 827381 |
Gramenei | AT4G16760.1; AT4G16760.1; AT4G16760 [O65202-1] |
KEGGi | ath:AT4G16760 |
Organism-specific databases
Araporti | AT4G16760 |
TAIRi | locus:2129121, AT4G16760 |
Phylogenomic databases
eggNOGi | KOG0136, Eukaryota |
InParanoidi | O65202 |
OMAi | NQMGHLL |
OrthoDBi | 416859at2759 |
PhylomeDBi | O65202 |
Enzyme and pathway databases
BioCyci | ARA:AT4G16760-MONOMER MetaCyc:AT4G16760-MONOMER |
BRENDAi | 1.3.3.6, 399 |
Miscellaneous databases
EvolutionaryTracei | O65202 |
PROi | PR:O65202 |
Gene expression databases
ExpressionAtlasi | O65202, baseline and differential |
Genevisiblei | O65202, AT |
Family and domain databases
Gene3Di | 1.10.540.10, 1 hit |
InterProi | View protein in InterPro IPR029320, Acyl-CoA_ox_N IPR006091, Acyl-CoA_Oxase/DH_cen-dom IPR012258, Acyl-CoA_oxidase IPR002655, Acyl-CoA_oxidase_C IPR036250, AcylCo_DH-like_C IPR037069, AcylCoA_DH/ox_N_sf IPR009100, AcylCoA_DH/oxidase_NM_dom |
Pfami | View protein in Pfam PF01756, ACOX, 1 hit PF02770, Acyl-CoA_dh_M, 1 hit PF14749, Acyl-CoA_ox_N, 1 hit |
PIRSFi | PIRSF000168, Acyl-CoA_oxidase, 1 hit |
SUPFAMi | SSF47203, SSF47203, 2 hits SSF56645, SSF56645, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACOX1_ARATH | |
Accessioni | O65202Primary (citable) accession number: O65202 Secondary accession number(s): O23518 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2005 |
Last sequence update: | August 1, 1998 | |
Last modified: | December 2, 2020 | |
This is version 153 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families