UniProtKB - O64697 (C7101_ARATH)
Protein
Cytochrome P450 710A1
Gene
CYP710A1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Required to form the C-22 double bond in the sterol side chain. Possesses in vitro C-22 desaturase activity toward beta-sitosterol and produces stigmasterol. No activity with campesterol.3 Publications
Miscellaneous
Plants overexpressing CYP710A1 show higher levels of stigmasterol and lower levels of beta-sitosterol than the wild-type.1 Publication
Catalytic activityi
- ergosta-5,7,24(28)-trien-3β-ol + H+ + NADPH + O2 = ergosta-5,7,22,24(28)-tetraen-3β-ol + 2 H2O + NADP+1 PublicationEC:1.14.19.411 Publication
Cofactori
hemeBy similarity
Kineticsi
kcat is 0.53 min(-1) for beta-sisterol.1 Publication
- KM=1 µM for beta-sisterol1 Publication
: sterol biosynthesis Pathwayi
This protein is involved in the pathway sterol biosynthesis, which is part of Steroid biosynthesis.1 PublicationView all proteins of this organism that are known to be involved in the pathway sterol biosynthesis and in Steroid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 434 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- C-22 sterol desaturase activity Source: TAIR
- heme binding Source: InterPro
- iron ion binding Source: InterPro
- monooxygenase activity Source: UniProtKB-KW
- oxidoreductase activity Source: GO_Central
GO - Biological processi
- sterol biosynthetic process Source: UniProtKB-UniPathway
- sterol metabolic process Source: GO_Central
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Biological process | Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism |
Ligand | Heme, Iron, Metal-binding, NADP |
Enzyme and pathway databases
BioCyci | ARA:AT2G34500-MONOMER MetaCyc:AT2G34500-MONOMER |
UniPathwayi | UPA00766 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome P450 710A11 Publication (EC:1.14.19.411 Publication)Alternative name(s): C-22 sterol desaturase1 Publication |
Gene namesi | Name:CYP710A11 Publication Ordered Locus Names:At2g34500Imported ORF Names:F13P17.35Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G34500 |
TAIRi | locus:2040939, AT2G34500 |
Subcellular locationi
Other locations
- Membrane Curated; Single-pass membrane protein Curated
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 5 – 25 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
MembranePathology & Biotechi
Disruption phenotypei
No visible phenotype under normal growth condition, but upon infection with P.syringae, no accumulation of stigmasterol and increased resistance to the pathogen.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000411205 | 1 – 495 | Cytochrome P450 710A1Add BLAST | 495 |
Proteomic databases
PaxDbi | O64697 |
PRIDEi | O64697 |
ProteomicsDBi | 239141 |
Expressioni
Tissue specificityi
Expressed in the vascular tissues of roots, shoots and leaves. Expressed in root tips and sepals. Very low expression in stems and siliques.1 Publication
Inductioni
By infection with Pseudomonas syringae.1 Publication
Gene expression databases
ExpressionAtlasi | O64697, baseline and differential |
Genevisiblei | O64697, AT |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome P450 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0157, Eukaryota |
HOGENOMi | CLU_023517_1_0_1 |
InParanoidi | O64697 |
OMAi | CLARKYV |
OrthoDBi | 574756at2759 |
PhylomeDBi | O64697 |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
i Sequence
Sequence statusi: Complete.
O64697-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVFSVSIFAS LAPYLISAFL LFLLVEQLSY LFKKRNIPGP FFVPPIIGNA
60 70 80 90 100
VALVRDPTSF WDKQSSTANI SGLSANYLIG KFIVYIRDTE LSHQIFSNVR
110 120 130 140 150
PDAFHLIGHP FGKKLFGDHN LIYMFGEDHK SVRRQLAPNF TPKALSTYSA
160 170 180 190 200
LQQLVILRHL RQWEGSTSGG SRPVSLRQLV RELNLETSQT VFVGPYLDKE
210 220 230 240 250
AKNRFRTDYN LFNLGSMALP IDLPGFAFGE ARRAVKRLGE TLGICAGKSK
260 270 280 290 300
ARMAAGEEPA CLIDFWMQAI VAENPQPPHS GDEEIGGLLF DFLFAAQDAS
310 320 330 340 350
TSSLLWAVTL LDSEPEVLNR VREEVAKIWS PESNALITVD QLAEMKYTRS
360 370 380 390 400
VAREVIRYRP PATMVPHVAA IDFPLTETYT IPKGTIVFPS VFDSSFQGFT
410 420 430 440 450
EPDRFDPDRF SETRQEDQVF KRNFLAFGWG PHQCVGQRYA LNHLVLFIAM
460 470 480 490
FSSLLDFKRL RSDGCDEIVY CPTISPKDGC TVFLSRRVAK YPNFS
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB219423 Genomic DNA Translation: BAE71351.1 AC004077 Genomic DNA Translation: AAC26690.1 AC004481 Genomic DNA Translation: AAM14944.1 CP002685 Genomic DNA Translation: AEC08982.1 BT002069 mRNA Translation: AAN72080.1 BT010554 mRNA Translation: AAQ65177.1 |
PIRi | T02337 |
RefSeqi | NP_180997.1, NM_129002.3 |
Genome annotation databases
EnsemblPlantsi | AT2G34500.1; AT2G34500.1; AT2G34500 |
GeneIDi | 818013 |
Gramenei | AT2G34500.1; AT2G34500.1; AT2G34500 |
KEGGi | ath:AT2G34500 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB219423 Genomic DNA Translation: BAE71351.1 AC004077 Genomic DNA Translation: AAC26690.1 AC004481 Genomic DNA Translation: AAM14944.1 CP002685 Genomic DNA Translation: AEC08982.1 BT002069 mRNA Translation: AAN72080.1 BT010554 mRNA Translation: AAQ65177.1 |
PIRi | T02337 |
RefSeqi | NP_180997.1, NM_129002.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 3702.AT2G34500.1 |
Proteomic databases
PaxDbi | O64697 |
PRIDEi | O64697 |
ProteomicsDBi | 239141 |
Genome annotation databases
EnsemblPlantsi | AT2G34500.1; AT2G34500.1; AT2G34500 |
GeneIDi | 818013 |
Gramenei | AT2G34500.1; AT2G34500.1; AT2G34500 |
KEGGi | ath:AT2G34500 |
Organism-specific databases
Araporti | AT2G34500 |
TAIRi | locus:2040939, AT2G34500 |
Phylogenomic databases
eggNOGi | KOG0157, Eukaryota |
HOGENOMi | CLU_023517_1_0_1 |
InParanoidi | O64697 |
OMAi | CLARKYV |
OrthoDBi | 574756at2759 |
PhylomeDBi | O64697 |
Enzyme and pathway databases
UniPathwayi | UPA00766 |
BioCyci | ARA:AT2G34500-MONOMER MetaCyc:AT2G34500-MONOMER |
Miscellaneous databases
PROi | PR:O64697 |
Gene expression databases
ExpressionAtlasi | O64697, baseline and differential |
Genevisiblei | O64697, AT |
Family and domain databases
Gene3Di | 1.10.630.10, 1 hit |
InterProi | View protein in InterPro IPR001128, Cyt_P450 IPR017972, Cyt_P450_CS IPR002401, Cyt_P450_E_grp-I IPR036396, Cyt_P450_sf |
Pfami | View protein in Pfam PF00067, p450, 1 hit |
PRINTSi | PR00463, EP450I PR00385, P450 |
SUPFAMi | SSF48264, SSF48264, 1 hit |
PROSITEi | View protein in PROSITE PS00086, CYTOCHROME_P450, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | C7101_ARATH | |
Accessioni | O64697Primary (citable) accession number: O64697 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 27, 2011 |
Last sequence update: | August 1, 1998 | |
Last modified: | April 7, 2021 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families