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Protein

Stromal cell-derived factor 1

Gene

CXCL12

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Chemoattractant active on T-lymphocytes and monocytes but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Binds to the allosteric site (site 2) of integrins and activates integrins ITGAV:ITGB3, ITGA4:ITGB1 and ITGA5:ITGB1 in a CXCR4-independent manner. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Plays a protective role after myocardial infarction. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Stimulates the proliferation of bone marrow-derived B-cell progenitors in the presence of IL7 as well as growth of stromal cell-dependent pre-B-cells.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei45Important for integrin interaction and activationBy similarity1
Sitei46Important for dimer formationBy similarity1
Sitei48Important for integrin interaction and activationBy similarity1
Binding sitei62HeparinBy similarity1
Sitei64Important for integrin interaction and activationBy similarity1
Binding sitei69HeparinBy similarity1
Binding sitei85HeparinBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCytokine, Growth factor
Biological processChemotaxis

Names & Taxonomyi

Protein namesi
Recommended name:
Stromal cell-derived factor 1
Short name:
SDF-1
Alternative name(s):
C-X-C motif chemokine 12
Gene namesi
Name:CXCL12
Synonyms:SDF1
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
Proteomesi
  • UP000011712 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Sequence analysisAdd BLAST21
ChainiPRO_000000510822 – 93Stromal cell-derived factor 1Add BLAST72

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi30 ↔ 55By similarity
Disulfide bondi32 ↔ 71By similarity

Keywords - PTMi

Disulfide bond

Expressioni

Gene expression databases

BgeeiENSFCAG00000007670

Interactioni

Subunit structurei

Monomer or homodimer; in equilibrium. Dimer formation is induced by non acidic pH and the presence of multivalent anions, and by binding to CXCR4 or heparin. Monomeric form is required for full chemotactic activity and resistance to ischemia/reperfusion injury, whereas the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization but with no chemotactic activity and instead acts as a selective antagonist that blocks chemotaxis induced by the monomeric form. Interacts with the N-terminus of ACKR3. Interacts with integrin subunit ITGB3 (via the allosteric site (site 2)).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000007102

Structurei

3D structure databases

ProteinModelPortaliO62657
SMRiO62657
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 33Receptor and heparin bindingBy similarity5
Regioni39 – 41Receptor bindingBy similarity3
Regioni41 – 51Heparin bindingBy similarityAdd BLAST11
Regioni48 – 50Receptor bindingBy similarity3
Regioni60 – 70Receptor bindingBy similarityAdd BLAST11

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi22 – 23Receptor activation motifBy similarity2

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J1S5 Eukaryota
ENOG4112BS6 LUCA
HOVERGENiHBG107437
InParanoidiO62657
KOiK10031
OMAiSTPNCAL
OrthoDBiEOG091G14JB

Family and domain databases

CDDicd00273 Chemokine_CXC, 1 hit
InterProiView protein in InterPro
IPR001811 Chemokine_IL8-like_dom
IPR033899 CXC_Chemokine_domain
IPR036048 Interleukin_8-like_sf
PfamiView protein in Pfam
PF00048 IL8, 1 hit
SMARTiView protein in SMART
SM00199 SCY, 1 hit
SUPFAMiSSF54117 SSF54117, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Beta (identifier: O62657-1) [UniParc]FASTAAdd to basket
Also known as: SDF-1b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDAKVVAVLA LVLAALCLSD GKPVSLSYRC PCRFFESHVA RANVKHLKIL
60 70 80 90
NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNKR FKM
Length:93
Mass (Da):10,581
Last modified:August 1, 1998 - v1
Checksum:i44FC763711E9BE37
GO
Isoform Alpha (identifier: O62657-2) [UniParc]FASTAAdd to basket
Also known as: SDF-1a

The sequence of this isoform differs from the canonical sequence as follows:
     90-93: Missing.

Show »
Length:89
Mass (Da):10,018
Checksum:i81E9BE373B2F85FD
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_00105590 – 93Missing in isoform Alpha. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011965 mRNA Translation: BAA28601.1
AB011966 mRNA Translation: BAA28602.1
RefSeqiNP_001009847.1, NM_001009847.1 [O62657-1]

Genome annotation databases

GeneIDi493806
KEGGifca:493806

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiSDF1_FELCA
AccessioniPrimary (citable) accession number: O62657
Secondary accession number(s): Q54AJ3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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