Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyruvate kinase

Gene

PyK

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (Gapdh1), Glyceraldehyde-3-phosphate dehydrogenase 2 (Gapdh2), Glyceraldehyde-3-phosphate dehydrogenase (Gapdh2), Glyceraldehyde-3-phosphate dehydrogenase (CG9010)
  2. Phosphoglycerate kinase (Pgk), Phosphoglycerate kinase, Phosphoglycerate kinase (Dmel\CG9961), Phosphoglycerate kinase (Dmel\CG9961), Phosphoglycerate kinase (Pgk), Phosphoglycerate kinase (CG9961)
  3. no protein annotated in this organism
  4. Enolase (Eno)
  5. Pyruvate kinase (CG7362), Pyruvate kinase (PyK), Pyruvate kinase (Dmel\CG2964), Pyruvate kinase (CG7069), Pyruvate kinase (Dmel\CG7069), Pyruvate kinase (CG2964)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei76SubstrateBy similarity1
Metal bindingi78PotassiumBy similarity1
Metal bindingi80PotassiumBy similarity1
Metal bindingi116PotassiumBy similarity1
Metal bindingi117Potassium; via carbonyl oxygenBy similarity1
Sitei273Transition state stabilizerBy similarity1
Metal bindingi275MagnesiumBy similarity1
Binding sitei298Substrate; via amide nitrogenBy similarity1
Metal bindingi299MagnesiumBy similarity1
Binding sitei299Substrate; via amide nitrogenBy similarity1
Binding sitei331SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • glucose homeostasis Source: FlyBase
  • glycolytic process Source: FlyBase
  • myoblast fusion Source: FlyBase
  • pyruvate metabolic process Source: FlyBase
  • response to sucrose Source: FlyBase
  • somatic muscle development Source: FlyBase

Keywordsi

Molecular functionKinase, Transferase
Biological processGlycolysis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

ReactomeiR-DME-6798695 Neutrophil degranulation
R-DME-70171 Glycolysis
UniPathwayiUPA00109; UER00188

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:PyK
ORF Names:CG7070
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0267385 PyK

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120991 – 533Pyruvate kinaseAdd BLAST533

Proteomic databases

PaxDbiO62619
PRIDEiO62619

Expressioni

Tissue specificityi

In adults expressed predominantly in the thorax.1 Publication

Developmental stagei

Low levels in larvae and pupae, but increases in young adults, becoming relatively stable in two-day-old flies.1 Publication

Gene expression databases

BgeeiFBgn0267385
GenevisibleiO62619 DM

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi67578, 30 interactors
DIPiDIP-19290N
IntActiO62619, 27 interactors
MINTiO62619
STRINGi7227.FBpp0083611

Structurei

3D structure databases

ProteinModelPortaliO62619
SMRiO62619
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiKOG2323 Eukaryota
COG0469 LUCA
GeneTreeiENSGT00390000008859
InParanoidiO62619
KOiK00873
OrthoDBiEOG091G0597
PhylomeDBiO62619

Family and domain databases

CDDicd00288 Pyruvate_Kinase, 1 hit
Gene3Di2.40.33.10, 1 hit
3.40.1380.20, 2 hits
InterProiView protein in InterPro
IPR001697 Pyr_Knase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
IPR011037 Pyrv_Knase-like_insert_dom_sf
IPR018209 Pyrv_Knase_AS
IPR015793 Pyrv_Knase_brl
IPR015795 Pyrv_Knase_C
IPR036918 Pyrv_Knase_C_sf
IPR015806 Pyrv_Knase_insert_dom_sf
PANTHERiPTHR11817 PTHR11817, 1 hit
PfamiView protein in Pfam
PF00224 PK, 1 hit
PF02887 PK_C, 1 hit
PRINTSiPR01050 PYRUVTKNASE
SUPFAMiSSF50800 SSF50800, 1 hit
SSF51621 SSF51621, 2 hits
SSF52935 SSF52935, 1 hit
TIGRFAMsiTIGR01064 pyruv_kin, 1 hit
PROSITEiView protein in PROSITE
PS00110 PYRUVATE_KINASE, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: O62619-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVNVTIYDEA PQLKPNEVPQ NMAAGADTQL EHMCRLQFDS PVPHVRLSGI
60 70 80 90 100
VCTIGPASSS VEMLEKMMAT GMNIARMNFS HGSHEYHAAT VANVRQAVKN
110 120 130 140 150
YSAKLGYEHP VAIALDTKGP EIRTGLIGGS GTAEIELKKG EKIKLTTNKE
160 170 180 190 200
FLEKGSLEIV YVDYENIVNV VKPGNRVFVD DGLISLIVRE VGKDSLTCEV
210 220 230 240 250
ENGGSLGSRK GVNLPGVPVD LPAVSEKDKS DLLFGVEQEV DMIFASFIRN
260 270 280 290 300
AAALTEIRKV LGEKGKNIKI ISKIENQQGM HNLDEIIEAG DGIMVARGDL
310 320 330 340 350
GIEIPAEKVF LAQKAMIARC NKAGKPVICA TQMLESMVKK PRPTRAEISD
360 370 380 390 400
VANAVLDGAD CVMLSGETAK GEYPLECVLT MAKTCKEAEA ALWHQNLFND
410 420 430 440 450
LVRGAGTIDA SHAAAIAAVE AATKAKASAI VVITTSGKSA FQVSKYRPRC
460 470 480 490 500
PIIAVTRFAQ TARQAHLYRG LVPLIYKEPG LGDWLKDVDV RVQFGLQVGK
510 520 530
KNGFIKTGDS VVVVTGWKQG SGFTNTIRIV TVE
Length:533
Mass (Da):57,440
Last modified:January 27, 2003 - v2
Checksum:iA8376B72F040EEDB
GO
Isoform B (identifier: O62619-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: Missing.

Note: No experimental confirmation available.
Show »
Length:512
Mass (Da):55,059
Checksum:i4ED4A31F0686874B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti180D → N in AAC16244 (Ref. 1) Curated1
Sequence conflicti180D → N in AAC15808 (Ref. 1) Curated1
Sequence conflicti271I → F in AAO24935 (PubMed:12537569).Curated1
Sequence conflicti397L → F in AAC16244 (Ref. 1) Curated1
Sequence conflicti397L → F in AAC15808 (Ref. 1) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0028851 – 21Missing in isoform B. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062478 Genomic DNA Translation: AAC16244.1
AF061507 mRNA Translation: AAC15808.1
AE014297 Genomic DNA Translation: AAF55979.3
AE014297 Genomic DNA Translation: AAN14373.1
AY118442 mRNA Translation: AAM48471.1
BT003180 mRNA Translation: AAO24935.1
BT082045 mRNA Translation: ACO95723.1
RefSeqiNP_524448.3, NM_079724.3 [O62619-1]
NP_732723.1, NM_170004.2 [O62619-2]
UniGeneiDm.7108

Genome annotation databases

EnsemblMetazoaiFBtr0084213; FBpp0083610; FBgn0267385 [O62619-2]
FBtr0084214; FBpp0083611; FBgn0267385 [O62619-1]
GeneIDi42620
KEGGidme:Dmel_CG7070

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKPYK_DROME
AccessioniPrimary (citable) accession number: O62619
Secondary accession number(s): C3KGI0
, Q86PE3, Q8MT14, Q9VD24
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 27, 2003
Last modified: July 18, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health