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Protein

Kunitz-type protein bli-5

Gene

bli-5

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Appears to lack serine protease inhibitor activity in vitro when tested with bovine pancreatic alpha-chymotrypsin and elastase (PubMed:19716386). Involved in cuticle biosynthesis (PubMed:16500660, PubMed:19716386).2 Publications

Caution

Appears to have serine protease activity in vitro (PubMed:19716386). However, it is uncertain if this activity is genuine as bli-5 lacks all the catalytic features of serine proteases.Curated1 Publication

GO - Molecular functioni

GO - Biological processi

Names & Taxonomyi

Protein namesi
Recommended name:
Kunitz-type protein bli-5Curated
Alternative name(s):
Blistered cuticle protein 5Imported
Kunitz-type protease inhibitor bli-51 Publication
Gene namesi
Name:bli-5Imported
ORF Names:F45G2.5Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiF45G2.5 ; CE16048 ; WBGene00000255 ; bli-5

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown causes blisters in the cuticle and abnormal localization of collagen col-19 in cuticle dorso/ventral annulae and lateral alea.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi56S → L in 518; blistered cuticle associated with abnormal localization of collagen col-19 in dorso/ventral annulae and lateral alea. Loss of struts separating the external and internal cuticle layers. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20Sequence analysisAdd BLAST20
ChainiPRO_500415999621 – 202Kunitz-type protein bli-5Sequence analysisAdd BLAST182

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi135 ↔ 184PROSITE-ProRule annotation
Disulfide bondi158 ↔ 180PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO62247

Expressioni

Tissue specificityi

Expressed in larval and adult hypodermis, hermaphrodite vulva and adult excretory cell and duct.1 Publication

Developmental stagei

Expressed at low levels in larvae and adults. Expression increases during L2-L3 molting stage an prior L3-L4 molting stage.1 Publication

Gene expression databases

BgeeiWBGene00000255 Expressed in 4 organ(s), highest expression level in material anatomical entity

Interactioni

Protein-protein interaction databases

STRINGi6239.F45G2.5

Structurei

3D structure databases

ProteinModelPortaliO62247
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini135 – 184BPTI/Kunitz inhibitorPROSITE-ProRule annotationAdd BLAST50

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4295 Eukaryota
ENOG410XQNP LUCA
HOGENOMiHOG000022035
InParanoidiO62247
OMAiFKWNPER
OrthoDBiEOG091G0L2Z

Family and domain databases

Gene3Di4.10.410.10, 1 hit
InterProiView protein in InterPro
IPR002223 Kunitz_BPTI
IPR036880 Kunitz_BPTI_sf
PfamiView protein in Pfam
PF00014 Kunitz_BPTI, 1 hit
SMARTiView protein in SMART
SM00131 KU, 1 hit
SUPFAMiSSF57362 SSF57362, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O62247-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVSIHNSFIL LMLMISICFC EKCLTNEECD LKWPDAICVR GRCRCSENTI
60 70 80 90 100
RKKSASREWV CLATNDATGN SGPPLTCPTP EGAGYQVMYR KDGEPVKCSS
110 120 130 140 150
KKKPDTCPEG FECIQGLSIL GALDGVCCPD RAKTCVHPIF DHPDDGYLSR
160 170 180 190 200
WGFDGEQCIE FKWNPERPSS ANNFKTRAHC EDYCIGSING ITNYHQSNFH

LF
Length:202
Mass (Da):22,633
Last modified:August 1, 1998 - v1
Checksum:iD366671FED7E354C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284603 Genomic DNA Translation: CAB07614.1
PIRiT22237
RefSeqiNP_499772.1, NM_067371.1
UniGeneiCel.10466

Genome annotation databases

EnsemblMetazoaiF45G2.5; F45G2.5; WBGene00000255
GeneIDi185812
KEGGicel:CELE_F45G2.5

Similar proteinsi

Entry informationi

Entry nameiBLI5_CAEEL
AccessioniPrimary (citable) accession number: O62247
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2017
Last sequence update: August 1, 1998
Last modified: September 12, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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