UniProtKB - O62090 (PRY1_CAEEL)
Axin-like protein pry-1
pry-1
Functioni
Works in parallel with axl-1 in negatively regulating bar-1 signaling in vulval precursor cells and Q neuroblasts. Inhibits Wnt signaling, which affects tissue specific expression of Hox genes, egl-5, lin-39 and mab-5. This in turn affects QR (postembryonic neuroblast) cell migration, vulval cell fate specification, and the development of sensory structures by the seam cell lineage. Has a role in alae V cell patterning, ray formation in the male tail and axon guidance. Does not affect B cell polarity.
7 PublicationsGO - Molecular functioni
- armadillo repeat domain binding Source: UniProtKB
- protein C-terminus binding Source: UniProtKB
GO - Biological processi
- anterior/posterior axis specification Source: UniProtKB
- anterior/posterior pattern specification Source: WormBase
- axon guidance Source: UniProtKB
- canonical Wnt signaling pathway Source: UniProtKB
- negative regulation of canonical Wnt signaling pathway Source: InterPro
- negative regulation of vulval development Source: WormBase
- negative regulation of Wnt signaling pathway Source: UniProtKB
- regulation of cell fate specification Source: WormBase
- regulation of protein localization Source: UniProtKB
Keywordsi
Molecular function | Developmental protein |
Biological process | Wnt signaling pathway |
Enzyme and pathway databases
Reactomei | R-CEL-195253, Degradation of beta-catenin by the destruction complex R-CEL-196299, Beta-catenin phosphorylation cascade R-CEL-4641262, Disassembly of the destruction complex and recruitment of AXIN to the membrane |
SignaLinki | O62090 |
Names & Taxonomyi
Protein namesi | Recommended name: Axin-like protein pry-1Alternative name(s): Protein polyray1 Publication |
Gene namesi | Name:pry-1Imported ORF Names:C37A5.9 |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | C37A5.9 ; CE30125 ; WBGene00004202 ; pry-1 |
Subcellular locationi
Plasma membrane
- Cell membrane 2 Publications
Nucleus
- Nucleus 2 Publications
Other locations
- cell cortex 2 Publications
Note: Subcellular location was measured using a GFP reporter gene. Location of the pry-1:GFP fusion protein ranges from plasma membrane and cytoplasmic dots to diffuse cytoplasmic and nuclear staining. This difference may be due to variations in expression levels of the fusion protein in different transgenic lines.2 Publications
Nucleus
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- cell cortex Source: WormBase
- cytoplasm Source: UniProtKB
- membrane Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000347254 | 1 – 586 | Axin-like protein pry-1Add BLAST | 586 |
Proteomic databases
EPDi | O62090 |
PaxDbi | O62090 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | WBGene00004202, Expressed in multi-cellular organism and 5 other tissues |
Interactioni
Subunit structurei
Interacts (via N-terminus) with apr-1 (via C-terminus).
Interacts with bar-1 (via ARM repeats), gsk-3, and mig-5.
2 PublicationsBinary interactionsi
O62090
With | #Exp. | IntAct |
---|---|---|
bar-1 [Q18825] | 3 | EBI-2917690,EBI-2528850 |
gsk-3 [Q9U2Q9] | 6 | EBI-2917690,EBI-330089 |
mig-5 [Q22227] | 4 | EBI-2917690,EBI-316403 |
GO - Molecular functioni
- armadillo repeat domain binding Source: UniProtKB
- protein C-terminus binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 38675, 39 interactors |
IntActi | O62090, 14 interactors |
STRINGi | 6239.C37A5.9 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 131 | RGSPROSITE-ProRule annotationAdd BLAST | 122 | |
Domaini | 505 – 586 | DIXPROSITE-ProRule annotationAdd BLAST | 82 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 135 | Required for interaction with apr-11 PublicationAdd BLAST | 135 | |
Regioni | 137 – 168 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 344 – 442 | DisorderedSequence analysisAdd BLAST | 99 | |
Regioni | 478 – 501 | DisorderedSequence analysisAdd BLAST | 24 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 346 – 360 | Polar residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 367 – 386 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 390 – 416 | Basic and acidic residuesSequence analysisAdd BLAST | 27 | |
Compositional biasi | 417 – 442 | Polar residuesSequence analysisAdd BLAST | 26 |
Phylogenomic databases
eggNOGi | ENOG502S33C, Eukaryota |
HOGENOMi | CLU_471192_0_0_1 |
InParanoidi | O62090 |
OMAi | CNATTSH |
OrthoDBi | 732602at2759 |
PhylomeDBi | O62090 |
Family and domain databases
Gene3Di | 1.10.167.10, 1 hit 2.40.240.130, 1 hit |
InterProi | View protein in InterPro IPR043581, Axin-like IPR001158, DIX IPR038207, DIX_dom_sf IPR016137, RGS IPR036305, RGS_sf IPR044926, RGS_subdomain_2 IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR46102, PTHR46102, 1 hit |
Pfami | View protein in Pfam PF00778, DIX, 1 hit PF00615, RGS, 1 hit |
SMARTi | View protein in SMART SM00315, RGS, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50841, DIX, 1 hit PS50132, RGS, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
METHLGWARS LEAVLSDRSA LDAFQEWLIE YSSPQYLDLF FAIRAYERMA
60 70 80 90 100
LEGKPEKSQL SKSIYSKFLS SRTGNCEAIP KHFRAPIGEK LRHGTELEDR
110 120 130 140 150
VFSHCSNFVQ EFLRRQHEEF VGSEEFIEAF NKMSSTTADQ LPGGSAHHSS
160 170 180 190 200
HQNTMRRSSG TTSRKSAAQI ATQLTAEALL KSKHDRHSKL GETKLEKMYP
210 220 230 240 250
PTRQPYVCNA TTSHNDSAVS STFSGDTPEA HRMHSNRLRH IRDEQARENH
260 270 280 290 300
GTMTLPRVEK ASVDGQQWDH SSESGRRNFA MEITRKLLRH IDKVKLNDEM
310 320 330 340 350
EKRIDDIEEC RYTTIDMVNG TEPNDDLGKI DEDEELDDYL KMKMTDDSQK
360 370 380 390 400
GSTNRSPKGP AGEPNKSGEG SKNTTLSPTN RAPAQLHNTI RVPRRKDYPR
410 420 430 440 450
DTSASLKSHR HHQIDTNRMM SQSMCAPSYS SASSSYSRDS FAPAPTTRVN
460 470 480 490 500
FAPGSSKSSQ FYDSSGIGSM APSAFSATSS LDYKDRRQHR KAPTPKKHSK
510 520 530 540 550
IGKNLSNLIT ISYLGTDKIP VVTHVPNDGP MTLAEFKRHF ALPNGAHQLF
560 570 580
FKTECEDGSA PFQLLLIKDE HHLLPVFEGR IAAELR
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF468834 mRNA Translation: AAL77082.1 Z92828 Genomic DNA Translation: CAB07332.2 |
PIRi | T19802 |
RefSeqi | NP_493474.2, NM_061073.5 |
Genome annotation databases
EnsemblMetazoai | C37A5.9.1; C37A5.9.1; WBGene00004202 |
GeneIDi | 173287 |
KEGGi | cel:CELE_C37A5.9 |
UCSCi | C37A5.9.1, c. elegans |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF468834 mRNA Translation: AAL77082.1 Z92828 Genomic DNA Translation: CAB07332.2 |
PIRi | T19802 |
RefSeqi | NP_493474.2, NM_061073.5 |
3D structure databases
SMRi | O62090 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 38675, 39 interactors |
IntActi | O62090, 14 interactors |
STRINGi | 6239.C37A5.9 |
Proteomic databases
EPDi | O62090 |
PaxDbi | O62090 |
Genome annotation databases
EnsemblMetazoai | C37A5.9.1; C37A5.9.1; WBGene00004202 |
GeneIDi | 173287 |
KEGGi | cel:CELE_C37A5.9 |
UCSCi | C37A5.9.1, c. elegans |
Organism-specific databases
CTDi | 173287 |
WormBasei | C37A5.9 ; CE30125 ; WBGene00004202 ; pry-1 |
Phylogenomic databases
eggNOGi | ENOG502S33C, Eukaryota |
HOGENOMi | CLU_471192_0_0_1 |
InParanoidi | O62090 |
OMAi | CNATTSH |
OrthoDBi | 732602at2759 |
PhylomeDBi | O62090 |
Enzyme and pathway databases
Reactomei | R-CEL-195253, Degradation of beta-catenin by the destruction complex R-CEL-196299, Beta-catenin phosphorylation cascade R-CEL-4641262, Disassembly of the destruction complex and recruitment of AXIN to the membrane |
SignaLinki | O62090 |
Miscellaneous databases
PROi | PR:O62090 |
Gene expression databases
Bgeei | WBGene00004202, Expressed in multi-cellular organism and 5 other tissues |
Family and domain databases
Gene3Di | 1.10.167.10, 1 hit 2.40.240.130, 1 hit |
InterProi | View protein in InterPro IPR043581, Axin-like IPR001158, DIX IPR038207, DIX_dom_sf IPR016137, RGS IPR036305, RGS_sf IPR044926, RGS_subdomain_2 IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR46102, PTHR46102, 1 hit |
Pfami | View protein in Pfam PF00778, DIX, 1 hit PF00615, RGS, 1 hit |
SMARTi | View protein in SMART SM00315, RGS, 1 hit |
SUPFAMi | SSF48097, SSF48097, 1 hit SSF54236, SSF54236, 1 hit |
PROSITEi | View protein in PROSITE PS50841, DIX, 1 hit PS50132, RGS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | PRY1_CAEEL | |
Accessioni | O62090Primary (citable) accession number: O62090 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 2, 2008 |
Last sequence update: | March 1, 2002 | |
Last modified: | February 23, 2022 | |
This is version 135 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase