UniProtKB - O61967 (LAP1_CAEEL)
Protein
Protein lap1
Gene
let-413
Organism
Caenorhabditis elegans
Status
Functioni
Critical role in assembling adherens junctions; adapter protein involved in polarizing protein trafficking in epithelial cells. Necessary to maintain, not establish, the entire terminal web (organelle-depleted, intermediate filament-rich layer of cytoplasm that underlies the apical microvilli of polarized epithelial cells) or brush border assembly at the apical surface gut cells. Required for correct localization of ifb-2 intermediate filaments in the terminal web. Required for dlg-1 lateral localization (PubMed:18411252). With dlg-1, cooperatively regulates ajm-1 localization to apical junctions (PubMed:11715019).4 Publications
GO - Biological processi
- actin filament-based process Source: WormBase
- adherens junction assembly Source: ARUK-UCL
- cell-cell adhesion Source: GO_Central
- cell-cell junction assembly Source: WormBase
- embryo development ending in birth or egg hatching Source: WormBase
- embryonic digestive tract morphogenesis Source: WormBase
- establishment or maintenance of cell polarity Source: UniProtKB
- establishment or maintenance of epithelial cell apical/basal polarity Source: GO_Central
- maintenance of epithelial cell apical/basal polarity Source: WormBase
- neurotransmitter receptor transport, endosome to postsynaptic membrane Source: GO_Central
- neurotransmitter receptor transport postsynaptic membrane to endosome Source: GO_Central
- protein transport Source: UniProtKB
- receptor clustering Source: GO_Central
- receptor localization to synapse Source: GO_Central
- regulation of intermediate filament polymerization or depolymerization Source: UniProtKB
Keywordsi
Biological process | Cell adhesion |
Names & Taxonomyi
Protein namesi | Recommended name: Protein lap1Alternative name(s): Lethal protein 413 |
Gene namesi | Name:let-413Imported ORF Names:F26D11.11Imported |
Organismi | Caenorhabditis elegans |
Taxonomic identifieri | 6239 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditina › Rhabditomorpha › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis |
Proteomesi |
|
Organism-specific databases
WormBasei | F26D11.11a ; CE29778 ; WBGene00002632 ; let-413 F26D11.11b ; CE37008 ; WBGene00002632 ; let-413 |
Subcellular locationi
Plasma membrane
- Basolateral cell membrane 3 Publications; Peripheral membrane protein 2 Publications
Note: Basolateral membrane of epithelial cells.2 Publications
Plasma Membrane
- basolateral plasma membrane Source: WormBase
Other locations
- adherens junction Source: GO_Central
- cell junction Source: GO_Central
- cell-cell junction Source: GO_Central
- postsynaptic density Source: GO_Central
- synapse Source: GO_Central
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Worms display a severely affected epithelial integrity, leading to abnormal morphogenesis of the pharynx, intestine and embryo.1 Publication
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 305 | P → L: Abolishes membrane localization. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000188305 | 1 – 699 | Protein lap1Add BLAST | 699 |
Proteomic databases
EPDi | O61967 |
PaxDbi | O61967 |
PeptideAtlasi | O61967 |
PTM databases
iPTMneti | O61967 |
Expressioni
Tissue specificityi
Expressed in the terminal web of the intestine (PubMed:15063180, PubMed:27506200). Expressed in seam cells (PubMed:27506200).2 Publications
Developmental stagei
Expressed in all embryonic epithelial cells.1 Publication
Gene expression databases
Bgeei | WBGene00002632, Expressed in pharyngeal muscle cell (C elegans) and 5 other tissues |
Interactioni
Protein-protein interaction databases
BioGRIDi | 44213, 12 interactors |
DIPi | DIP-25349N |
IntActi | O61967, 7 interactors |
STRINGi | 6239.F26D11.11b |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 37 – 59 | LRR 1Add BLAST | 23 | |
Repeati | 60 – 81 | LRR 2Add BLAST | 22 | |
Repeati | 83 – 104 | LRR 3Add BLAST | 22 | |
Repeati | 106 – 127 | LRR 4Add BLAST | 22 | |
Repeati | 129 – 150 | LRR 5Add BLAST | 22 | |
Repeati | 152 – 174 | LRR 6Add BLAST | 23 | |
Repeati | 175 – 196 | LRR 7Add BLAST | 22 | |
Repeati | 198 – 219 | LRR 8Add BLAST | 22 | |
Repeati | 221 – 242 | LRR 9Add BLAST | 22 | |
Repeati | 244 – 265 | LRR 10Add BLAST | 22 | |
Repeati | 267 – 288 | LRR 11Add BLAST | 22 | |
Repeati | 290 – 311 | LRR 12Add BLAST | 22 | |
Repeati | 313 – 334 | LRR 13Add BLAST | 22 | |
Repeati | 336 – 357 | LRR 14Add BLAST | 22 | |
Repeati | 359 – 380 | LRR 15Add BLAST | 22 | |
Repeati | 382 – 403 | LRR 16Add BLAST | 22 | |
Domaini | 584 – 665 | PDZPROSITE-ProRule annotationAdd BLAST | 82 |
Sequence similaritiesi
Belongs to the LAP (LRR and PDZ) protein family.Curated
Keywords - Domaini
Leucine-rich repeat, RepeatPhylogenomic databases
eggNOGi | KOG0619, Eukaryota KOG3528, Eukaryota |
GeneTreei | ENSGT00940000164066 |
HOGENOMi | CLU_000288_18_23_1 |
InParanoidi | O61967 |
OMAi | DIFRFRK |
OrthoDBi | 442171at2759 |
PhylomeDBi | O61967 |
Family and domain databases
Gene3Di | 2.30.42.10, 1 hit 3.80.10.10, 3 hits |
InterProi | View protein in InterPro IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR001478, PDZ IPR036034, PDZ_sf |
Pfami | View protein in Pfam PF13855, LRR_8, 3 hits PF00595, PDZ, 1 hit |
SMARTi | View protein in SMART SM00369, LRR_TYP, 12 hits SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS51450, LRR, 14 hits PS50106, PDZ, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform b (identifier: O61967-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPAFFCLPMA CQRQVDSIDR SQSNLQAIPS DIFRFRKLED LNLTMNNIKE
60 70 80 90 100
LDHRLFSLRH LRILDVSDNE LAVLPAEIGN LTQLIELNLN RNSIAKLPDT
110 120 130 140 150
MQNCKLLTTL NLSSNPFTRL PETICECSSI TILSLNETSL TLLPSNIGSL
160 170 180 190 200
TNLRVLEARD NLLRTIPLSI VELRKLEELD LGQNELEALP AEIGKLTSLR
210 220 230 240 250
EFYVDINSLT SLPDSISGCR MLDQLDVSEN QIIRLPENLG RMPNLTDLNI
260 270 280 290 300
SINEIIELPS SFGELKRLQM LKADRNSLHN LTSEIGKCQS LTELYLGQNF
310 320 330 340 350
LTDLPDTIGD LRQLTTLNVD CNNLSDIPDT IGNCKSLTVL SLRQNILTEL
360 370 380 390 400
PMTIGKCENL TVLDVASNKL PHLPFTVKVL YKLQALWLSE NQTQSILKLS
410 420 430 440 450
ETRDDRKGIK VVTCYLLPQV DAIDGEGRSG SAQHNTDRGA FLGGPKVHFH
460 470 480 490 500
DQADTTFEEN KEAEIHLGNF ERHNTPHPKT PKHKKGSIDG HMLPHEIDQP
510 520 530 540 550
RQLSLVSNHR TSTSSFGESS NSINRDLADI RAQNGVREAT LSPEREERMA
560 570 580 590 600
TSLSSLSNLA AGTQNMHTIR IQKDDTGKLG LSFAGGTSND PAPNSNGDSG
610 620 630 640 650
LFVTKVTPGS AAYRCGLREG DKLIRANDVN MINASQDNAM EAIKKRETVE
660 670 680 690
LVVLRRSPSP VSRTSEPSLN GSSHQLNHFD AGSPDSTMFV TSSTPVYAS
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A5K1I804 | A0A5K1I804_CAEEL | PDZ domain-containing protein | let-413 CELE_F26D11.11, F26D11.11 | 682 | Annotation score: | ||
A0A5K1IAV7 | A0A5K1IAV7_CAEEL | PDZ domain-containing protein | let-413 CELE_F26D11.11, F26D11.11 | 689 | Annotation score: | ||
A0A5K1IC26 | A0A5K1IC26_CAEEL | PDZ domain-containing protein | let-413 CELE_F26D11.11, F26D11.11 | 706 | Annotation score: | ||
A0A5K1I8A6 | A0A5K1I8A6_CAEEL | PDZ domain-containing protein | let-413 CELE_F26D11.11, F26D11.11 | 669 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_015673 | 531 | R → RFIDAPAS in isoform a. 1 Publication | 1 | |
Alternative sequenceiVSP_015674 | 666 – 699 | EPSLN…PVYAS → VSRPCEY in isoform a. 1 PublicationAdd BLAST | 34 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ276590 mRNA Translation: CAB91651.1 FO081227 Genomic DNA Translation: CCD70053.1 FO081227 Genomic DNA Translation: CCD70054.1 |
PIRi | T33295 |
RefSeqi | NP_001023850.1, NM_001028679.3 NP_001023851.1, NM_001028680.3 [O61967-1] |
Genome annotation databases
EnsemblMetazoai | F26D11.11a.1; F26D11.11a.1; WBGene00002632 [O61967-2] F26D11.11b.1; F26D11.11b.1; WBGene00002632 [O61967-1] |
GeneIDi | 179171 |
UCSCi | F26D11.11a, c. elegans [O61967-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ276590 mRNA Translation: CAB91651.1 FO081227 Genomic DNA Translation: CCD70053.1 FO081227 Genomic DNA Translation: CCD70054.1 |
PIRi | T33295 |
RefSeqi | NP_001023850.1, NM_001028679.3 NP_001023851.1, NM_001028680.3 [O61967-1] |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 44213, 12 interactors |
DIPi | DIP-25349N |
IntActi | O61967, 7 interactors |
STRINGi | 6239.F26D11.11b |
PTM databases
iPTMneti | O61967 |
Proteomic databases
EPDi | O61967 |
PaxDbi | O61967 |
PeptideAtlasi | O61967 |
Genome annotation databases
EnsemblMetazoai | F26D11.11a.1; F26D11.11a.1; WBGene00002632 [O61967-2] F26D11.11b.1; F26D11.11b.1; WBGene00002632 [O61967-1] |
GeneIDi | 179171 |
UCSCi | F26D11.11a, c. elegans [O61967-1] |
Organism-specific databases
CTDi | 179171 |
WormBasei | F26D11.11a ; CE29778 ; WBGene00002632 ; let-413 F26D11.11b ; CE37008 ; WBGene00002632 ; let-413 |
Phylogenomic databases
eggNOGi | KOG0619, Eukaryota KOG3528, Eukaryota |
GeneTreei | ENSGT00940000164066 |
HOGENOMi | CLU_000288_18_23_1 |
InParanoidi | O61967 |
OMAi | DIFRFRK |
OrthoDBi | 442171at2759 |
PhylomeDBi | O61967 |
Miscellaneous databases
PROi | PR:O61967 |
Gene expression databases
Bgeei | WBGene00002632, Expressed in pharyngeal muscle cell (C elegans) and 5 other tissues |
Family and domain databases
Gene3Di | 2.30.42.10, 1 hit 3.80.10.10, 3 hits |
InterProi | View protein in InterPro IPR001611, Leu-rich_rpt IPR003591, Leu-rich_rpt_typical-subtyp IPR032675, LRR_dom_sf IPR001478, PDZ IPR036034, PDZ_sf |
Pfami | View protein in Pfam PF13855, LRR_8, 3 hits PF00595, PDZ, 1 hit |
SMARTi | View protein in SMART SM00369, LRR_TYP, 12 hits SM00228, PDZ, 1 hit |
SUPFAMi | SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS51450, LRR, 14 hits PS50106, PDZ, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | LAP1_CAEEL | |
Accessioni | O61967Primary (citable) accession number: O61967 Secondary accession number(s): Q6A580, Q9NFN7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2004 |
Last sequence update: | September 27, 2005 | |
Last modified: | February 10, 2021 | |
This is version 164 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Caenorhabditis annotation project |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Caenorhabditis elegans
Caenorhabditis elegans: entries, gene names and cross-references to WormBase - SIMILARITY comments
Index of protein domains and families