UniProtKB - O61734 (CYCL_DROME)
Protein
Protein cycle
Gene
cyc
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
Putative transcription factor involved in the generation of biological rhythms. Activates cycling transcription of Period (PER) and Timeless (TIM) by binding to the E-box (5'-CACGTG-3') present in their promoters.
GO - Molecular functioni
- DNA binding Source: FlyBase
- DNA-binding transcription factor activity Source: InterPro
- protein heterodimerization activity Source: FlyBase
GO - Biological processi
- behavioral response to cocaine Source: FlyBase
- circadian regulation of gene expression Source: FlyBase
- circadian regulation of heart rate Source: FlyBase
- circadian rhythm Source: FlyBase
- eclosion rhythm Source: FlyBase
- locomotor rhythm Source: FlyBase
- positive regulation of transcription, DNA-templated Source: FlyBase
- positive regulation of transcription by RNA polymerase II Source: FlyBase
- regulation of circadian sleep/wake cycle, sleep Source: FlyBase
- response to starvation Source: FlyBase
- rhythmic behavior Source: FlyBase
Keywordsi
Molecular function | DNA-binding |
Biological process | Biological rhythms, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-DME-432395, Degradation of TIM R-DME-432408, Transcription regulation of cwo gene R-DME-432501, Transcription repression by PER and activation by PDP1 R-DME-432524, Degradation of PER R-DME-432560, Transcription activation by CLK:CYC and repression by VRI R-DME-432620, Dephosphorylation of PER R-DME-432626, Circadian Clock pathway |
Names & Taxonomyi
Protein namesi | Recommended name: Protein cycleAlternative name(s): Brain and muscle ARNT-like 1 Short name: BMAL1 MOP3 |
Gene namesi | Name:cyc ORF Names:CG8727 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0023094, cyc |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Nucleus
- nucleoplasm Source: Reactome
Other locations
- cytoplasm Source: InterPro
- transcription regulator complex Source: InterPro
Keywords - Cellular componenti
NucleusPathology & Biotechi
Disruption phenotypei
Cyc mutants don't display PER and TIM cycling, and are completely arrhythmic.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127165 | 1 – 413 | Protein cycleAdd BLAST | 413 |
Proteomic databases
PaxDbi | O61734 |
PRIDEi | O61734 |
Expressioni
Tissue specificityi
Expressed in head and ovary.
Gene expression databases
Bgeei | FBgn0023094, Expressed in egg chamber and 44 other tissues |
Genevisiblei | O61734, DM |
Interactioni
Subunit structurei
Efficient DNA binding requires dimerization with another bHLH protein.
Forms a heterodimer with Clock in order to activate PER and TIM transcription.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 35 | Interaction with E-box DNABy similarity | 1 | |
Sitei | 38 | Interaction with E-box DNABy similarity | 1 | |
Sitei | 39 | Interaction with E-box DNABy similarity | 1 | |
Sitei | 43 | Interaction with E-box DNABy similarity | 1 |
Binary interactionsi
O61734
With | #Exp. | IntAct |
---|---|---|
Clk [O61735] | 3 | EBI-87683,EBI-143834 |
GO - Molecular functioni
- protein heterodimerization activity Source: FlyBase
Protein-protein interaction databases
BioGRIDi | 65431, 10 interactors |
IntActi | O61734, 7 interactors |
MINTi | O61734 |
STRINGi | 7227.FBpp0074693 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O61734 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 83 | bHLHPROSITE-ProRule annotationAdd BLAST | 54 | |
Domaini | 104 – 175 | PAS 1PROSITE-ProRule annotationAdd BLAST | 72 | |
Domaini | 297 – 367 | PAS 2PROSITE-ProRule annotationAdd BLAST | 71 | |
Domaini | 372 – 413 | PACAdd BLAST | 42 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 228 – 234 | Poly-Ser | 7 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3561, Eukaryota |
HOGENOMi | CLU_011864_0_0_1 |
InParanoidi | O61734 |
OMAi | SWFDVLH |
OrthoDBi | 331262at2759 |
PhylomeDBi | O61734 |
Family and domain databases
CDDi | cd00083, HLH, 1 hit cd00130, PAS, 2 hits |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR001067, Nuc_translocat IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF00989, PAS, 1 hit |
PRINTSi | PR00785, NCTRNSLOCATR |
SMARTi | View protein in SMART SM00353, HLH, 1 hit SM00091, PAS, 2 hits |
SUPFAMi | SSF47459, SSF47459, 1 hit SSF55785, SSF55785, 2 hits |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS50112, PAS, 2 hits |
i Sequence
Sequence statusi: Complete.
O61734-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEVQEFCENM EEIEDENYDE EKSARTSDEN RKQNHSEIEK RRRDKMNTYI
60 70 80 90 100
NELSSMIPMC FAMQRKLDKL TVLRMAVQHL RGIRGSGSLH PFNGSDYRPS
110 120 130 140 150
FLSDQELKMI ILQASEGFLF VVGCDRGRIL YVSDSVSSVL NSTQADLLGQ
160 170 180 190 200
SWFDVLHPKD IGKVKEQLSS LEQCPRERLI DAKTMLPVKT DVPQSLCRLC
210 220 230 240 250
PGARRSFFCR MKLRTASNNQ IKEESDTSSS SRSSTKRKSR LTTGHKYRVI
260 270 280 290 300
QCTGYLKSWT PIKDEDQDAD SDEQTTNLSC LVAIGRIPPN VRNSTVPASL
310 320 330 340 350
DNHPNIRHVL FISRHSGEGK FLFIDQRATL VIGFLPQEIL GTSFYEYFHN
360 370 380 390 400
EDIAALMESH KMVMQVPEKV TTQVYRFRCK DNSYIQLQSE WRAFKNPWTS
410
EIDYIIAKNS VFL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 242 | T → S in AAC39124 (PubMed:9630224).Curated | 1 | |
Sequence conflicti | 242 | T → S in AAD10629 (PubMed:9616122).Curated | 1 | |
Sequence conflicti | 242 | T → S in AAC62235 (Ref. 3) Curated | 1 | |
Sequence conflicti | 242 | T → S in AAM51144 (PubMed:12537569).Curated | 1 | |
Sequence conflicti | 308 | H → Y in AAC39124 (PubMed:9630224).Curated | 1 | |
Sequence conflicti | 362 | M → V in AAC39124 (PubMed:9630224).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF065473 mRNA Translation: AAC39124.1 AF067206 mRNA Translation: AAD10629.1 AF069998 mRNA Translation: AAC62235.1 AE014296 Genomic DNA Translation: AAF49107.1 AY119284 mRNA Translation: AAM51144.1 BT025197 mRNA Translation: ABF17888.1 |
RefSeqi | NP_524168.2, NM_079444.3 |
Genome annotation databases
EnsemblMetazoai | FBtr0074924; FBpp0074693; FBgn0023094 |
GeneIDi | 40162 |
KEGGi | dme:Dmel_CG8727 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF065473 mRNA Translation: AAC39124.1 AF067206 mRNA Translation: AAD10629.1 AF069998 mRNA Translation: AAC62235.1 AE014296 Genomic DNA Translation: AAF49107.1 AY119284 mRNA Translation: AAM51144.1 BT025197 mRNA Translation: ABF17888.1 |
RefSeqi | NP_524168.2, NM_079444.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5F5Y | X-ray | 2.20 | A | 311-412 | [»] | |
5F68 | X-ray | 1.23 | A | 311-412 | [»] | |
5F69 | X-ray | 1.37 | A | 311-412 | [»] | |
5F6A | X-ray | 1.93 | A | 311-412 | [»] | |
SMRi | O61734 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 65431, 10 interactors |
IntActi | O61734, 7 interactors |
MINTi | O61734 |
STRINGi | 7227.FBpp0074693 |
Proteomic databases
PaxDbi | O61734 |
PRIDEi | O61734 |
Genome annotation databases
EnsemblMetazoai | FBtr0074924; FBpp0074693; FBgn0023094 |
GeneIDi | 40162 |
KEGGi | dme:Dmel_CG8727 |
Organism-specific databases
CTDi | 40162 |
FlyBasei | FBgn0023094, cyc |
Phylogenomic databases
eggNOGi | KOG3561, Eukaryota |
HOGENOMi | CLU_011864_0_0_1 |
InParanoidi | O61734 |
OMAi | SWFDVLH |
OrthoDBi | 331262at2759 |
PhylomeDBi | O61734 |
Enzyme and pathway databases
Reactomei | R-DME-432395, Degradation of TIM R-DME-432408, Transcription regulation of cwo gene R-DME-432501, Transcription repression by PER and activation by PDP1 R-DME-432524, Degradation of PER R-DME-432560, Transcription activation by CLK:CYC and repression by VRI R-DME-432620, Dephosphorylation of PER R-DME-432626, Circadian Clock pathway |
Miscellaneous databases
BioGRID-ORCSi | 40162, 0 hits in 3 CRISPR screens |
GenomeRNAii | 40162 |
PROi | PR:O61734 |
Gene expression databases
Bgeei | FBgn0023094, Expressed in egg chamber and 44 other tissues |
Genevisiblei | O61734, DM |
Family and domain databases
CDDi | cd00083, HLH, 1 hit cd00130, PAS, 2 hits |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR001067, Nuc_translocat IPR000014, PAS IPR035965, PAS-like_dom_sf IPR013767, PAS_fold |
Pfami | View protein in Pfam PF00010, HLH, 1 hit PF00989, PAS, 1 hit |
PRINTSi | PR00785, NCTRNSLOCATR |
SMARTi | View protein in SMART SM00353, HLH, 1 hit SM00091, PAS, 2 hits |
SUPFAMi | SSF47459, SSF47459, 1 hit SSF55785, SSF55785, 2 hits |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit PS50112, PAS, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYCL_DROME | |
Accessioni | O61734Primary (citable) accession number: O61734 Secondary accession number(s): O76344 Q9VW44 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | July 10, 2007 | |
Last modified: | February 10, 2021 | |
This is version 180 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Drosophila
Drosophila: entries, gene names and cross-references to FlyBase - PDB cross-references
Index of Protein Data Bank (PDB) cross-references