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UniProtKB - O61668 (SIX1_MESMA)
Protein
Beta-insect excitatory toxin BmKIT1
Gene
N/A
Organism
Mesobuthus martensii (Manchurian scorpion) (Buthus martensii)
Status
Functioni
Excitatory insect beta-toxins induce a spastic paralysis. They bind voltage-independently at site-4 of sodium channels (Nav) and shift the voltage of activation toward more negative potentials thereby affecting sodium channel activation and promoting spontaneous and repetitive firing. This toxin is active only on insects.
1 PublicationGO - Molecular functioni
- sodium channel inhibitor activity Source: InterPro
- toxin activity Source: UniProtKB-KW
GO - Biological processi
- defense response Source: InterPro
Keywordsi
Molecular function | Ion channel impairing toxin, Neurotoxin, Toxin, Voltage-gated sodium channel impairing toxin |
Protein family/group databases
TCDBi | 8.B.1.1.5, the long (4c-c) scorpion toxin (l-st) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-insect excitatory toxin BmKIT1Alternative name(s): Bm32-VI1 Publication BmK IT11 Publication Short name: BmK IT1 PublicationImported Short name: BmKITImported BmK-betaIT |
Organismi | Mesobuthus martensii (Manchurian scorpion) (Buthus martensii) |
Taxonomic identifieri | 34649 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Chelicerata › Arachnida › Scorpiones › Buthida › Buthoidea › Buthidae › Mesobuthus |
Subcellular locationi
Extracellular region or secreted
- Secreted 1 Publication
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPathology & Biotechi
Toxic dosei
PD50 is 0.18 ng/mg of Gryllus bimaculatus crickets.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | 2 PublicationsAdd BLAST | 18 | |
ChainiPRO_0000035200 | 19 – 87 | Beta-insect excitatory toxin BmKIT12 PublicationsAdd BLAST | 69 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 34 ↔ 55 | Combined sources1 Publication | ||
Disulfide bondi | 40 ↔ 60 | Combined sources1 Publication | ||
Disulfide bondi | 44 ↔ 62 | Combined sources1 Publication | ||
Disulfide bondi | 56 ↔ 82 | Combined sources1 Publication | ||
Modified residuei | 87 | Isoleucine amide1 Publication | 1 |
Keywords - PTMi
Amidation, Disulfide bondExpressioni
Tissue specificityi
Expressed by the venom gland.1 Publication
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | O61668 |
SMRi | O61668 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O61668 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 20 – 83 | LCN-type CS-alpha/betaPROSITE-ProRule annotationAdd BLAST | 64 |
Domaini
Has the structural arrangement of an alpha-helix connected to antiparallel beta-sheets by disulfide bonds (CS-alpha/beta).Curated
Sequence similaritiesi
Belongs to the long (4 C-C) scorpion toxin superfamily. Sodium channel inhibitor family. Beta subfamily.Curated
Keywords - Domaini
SignalFamily and domain databases
Gene3Di | 3.30.30.10, 1 hit |
InterProi | View protein in InterPro IPR044062, LCN-type_CS_alpha_beta_dom IPR003614, Scorpion_toxin-like IPR036574, Scorpion_toxin-like_sf IPR002061, Scorpion_toxinL/defensin |
Pfami | View protein in Pfam PF00537, Toxin_3, 1 hit |
SMARTi | View protein in SMART SM00505, Knot1, 1 hit |
SUPFAMi | SSF57095, SSF57095, 1 hit |
PROSITEi | View protein in PROSITE PS51863, LCN_CSAB, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O61668-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKFFLIFLVI FPIMGVLGKK NGYAVDSSGK VSECLLNNYC NNICTKVYYA
60 70 80
TSGYCCLLSC YCFGLDDDKA VLKIKDATKS YCDVQIIG
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 87 | I → N in CAA76604 (Ref. 4) Curated | 1 |
Mass spectrometryi
Molecular mass is 7632.83 Da. Determined by MALDI. 1 Publication
Molecular mass is 7634.0 Da. Determined by ESI. 1 Publication
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF057555 mRNA Translation: AAC14130.1 Y17050 mRNA Translation: CAA76604.1 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF057555 mRNA Translation: AAC14130.1 Y17050 mRNA Translation: CAA76604.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1WWN | NMR | - | A | 19-87 | [»] | |
AlphaFoldDBi | O61668 | |||||
SMRi | O61668 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein family/group databases
TCDBi | 8.B.1.1.5, the long (4c-c) scorpion toxin (l-st) superfamily |
Miscellaneous databases
EvolutionaryTracei | O61668 |
Family and domain databases
Gene3Di | 3.30.30.10, 1 hit |
InterProi | View protein in InterPro IPR044062, LCN-type_CS_alpha_beta_dom IPR003614, Scorpion_toxin-like IPR036574, Scorpion_toxin-like_sf IPR002061, Scorpion_toxinL/defensin |
Pfami | View protein in Pfam PF00537, Toxin_3, 1 hit |
SMARTi | View protein in SMART SM00505, Knot1, 1 hit |
SUPFAMi | SSF57095, SSF57095, 1 hit |
PROSITEi | View protein in PROSITE PS51863, LCN_CSAB, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SIX1_MESMA | |
Accessioni | O61668Primary (citable) accession number: O61668 Secondary accession number(s): O61995 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | August 1, 1998 | |
Last modified: | May 25, 2022 | |
This is version 99 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Animal Toxin Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families