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Protein

mRNA-capping enzyme

Gene

RNGTT

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.3 Publications

Miscellaneous

Isoform 2 to isoform 4 lack mRNA 5'-guanylyltransferase activity due to disruptions of the GTase domain.

Catalytic activityi

A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.
GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei126For RNA 5'-triphosphatase activityBy similarity1
Active sitei294N6-GMP-lysine intermediate1 Publication1
Binding sitei299GTPBy similarity1
Binding sitei315GTPSequence analysis1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi343 – 345GTPSequence analysis3
Nucleotide bindingi458 – 460GTPSequence analysis3
Nucleotide bindingi528 – 533GTPSequence analysis6

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Nucleotidyltransferase, Transferase
Biological processHost-virus interaction, mRNA capping, mRNA processing
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS03479-MONOMER
BRENDAi2.7.7.50 2681
ReactomeiR-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-72086 mRNA Capping
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
mRNA-capping enzyme
Alternative name(s):
HCAP1
HCE
Including the following 2 domains:
Polynucleotide 5'-triphosphatase (EC:3.1.3.33)
Alternative name(s):
mRNA 5'-triphosphatase
Short name:
TPase
mRNA guanylyltransferase (EC:2.7.7.50)
Alternative name(s):
GTP--RNA guanylyltransferase
Short name:
GTase
Gene namesi
Name:RNGTT
Synonyms:CAP1A
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000111880.15
HGNCiHGNC:10073 RNGTT
MIMi603512 gene
neXtProtiNX_O60942

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi234E → A: No effect on GTase activity. 1 Publication1
Mutagenesisi294K → A: Loss of GTase activity. 1 Publication1
Mutagenesisi299R → A: Loss of GTase activity. 1 Publication1
Mutagenesisi345E → A: Loss of GTase activity. 1 Publication1
Mutagenesisi458K → A: Loss of GTase activity. 2 Publications1
Mutagenesisi460K → A: Loss of GTase activity. 2 Publications1
Mutagenesisi528R → A: Strongly reduced GTase activity. 1 Publication1
Mutagenesisi530R → A: Loss of GTase activity. 1 Publication1
Mutagenesisi532D → A: Loss of GTase activity. 1 Publication1
Mutagenesisi533K → A: Loss of GTase activity. 1 Publication1

Organism-specific databases

DisGeNETi8732
OpenTargetsiENSG00000111880
PharmGKBiPA34446

Chemistry databases

DrugBankiDB02325 Isopropyl Alcohol

Polymorphism and mutation databases

BioMutaiRNGTT

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002101081 – 597mRNA-capping enzymeAdd BLAST597

Proteomic databases

EPDiO60942
MaxQBiO60942
PaxDbiO60942
PeptideAtlasiO60942
PRIDEiO60942
ProteomicsDBi49688
49689 [O60942-2]
49690 [O60942-3]
49691 [O60942-4]

PTM databases

DEPODiO60942
iPTMnetiO60942
PhosphoSitePlusiO60942

Expressioni

Tissue specificityi

Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle.

Gene expression databases

BgeeiENSG00000111880 Expressed in 221 organ(s), highest expression level in tonsil
CleanExiHS_RNGTT
GenevisibleiO60942 HS

Organism-specific databases

HPAiHPA003750

Interactioni

Subunit structurei

Interacts with POLR2A (via C-terminus); this enhances guanylyltransferase activity. Binds (via GTase domain) to the elongating phosphorylated form of RNA polymerase II; can form direct interactions with the phosphorylated POLR2A C-terminal domain and indirect interactions via bound RNA (By similarity). Interacts with SUPT5H and RNMT. Interacts with HIV-1 Tat.By similarity2 Publications
(Microbial infection) Interacts with HIV-1 Tat.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
NCK1P16333-12EBI-1237132,EBI-15578122

Protein-protein interaction databases

BioGridi114270, 55 interactors
DIPiDIP-61014N
IntActiO60942, 6 interactors
MINTiO60942
STRINGi9606.ENSP00000358497

Structurei

Secondary structure

1597
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO60942
SMRiO60942
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60942

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 212TPaseAdd BLAST212
Regioni229 – 597GTaseAdd BLAST369
Regioni330 – 386Interaction with POLR2ABy similarityAdd BLAST57

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi195 – 205Asp/Glu-richAdd BLAST11

Sequence similaritiesi

In the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family.Curated
In the C-terminal section; belongs to the eukaryotic GTase family.Curated

Phylogenomic databases

eggNOGiKOG2386 Eukaryota
COG5226 LUCA
GeneTreeiENSGT00530000063473
HOVERGENiHBG006332
InParanoidiO60942
KOiK13917
OMAiPELPEWC
OrthoDBiEOG091G063D
PhylomeDBiO60942
TreeFamiTF314914

Family and domain databases

Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR017074 mRNA_cap_enz_bifunc
IPR001339 mRNA_cap_enzyme
IPR013846 mRNA_cap_enzyme_C
IPR012340 NA-bd_OB-fold
IPR029021 Prot-tyrosine_phosphatase-like
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
PF03919 mRNA_cap_C, 1 hit
PF01331 mRNA_cap_enzyme, 1 hit
PIRSFiPIRSF036958 mRNA_capping_HCE, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O60942-1) [UniParc]FASTAAdd to basket
Also known as: HCE1, HCAP1A

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAHNKIPPRW LNCPRRGQPV AGRFLPLKTM LGPRYDSQVA EENRFHPSML
60 70 80 90 100
SNYLKSLKVK MGLLVDLTNT SRFYDRNDIE KEGIKYIKLQ CKGHGECPTT
110 120 130 140 150
ENTETFIRLC ERFNERNPPE LIGVHCTHGF NRTGFLICAF LVEKMDWSIE
160 170 180 190 200
AAVATFAQAR PPGIYKGDYL KELFRRYGDI EEAPPPPLLP DWCFEDDEDE
210 220 230 240 250
DEDEDGKKES EPGSSASFGK RRKERLKLGA IFLEGVTVKG VTQVTTQPKL
260 270 280 290 300
GEVQQKCHQF CGWEGSGFPG AQPVSMDKQN IKLLDLKPYK VSWKADGTRY
310 320 330 340 350
MMLIDGTNEV FMIDRDNSVF HVSNLEFPFR KDLRMHLSNT LLDGEMIIDR
360 370 380 390 400
VNGQAVPRYL IYDIIKFNSQ PVGDCDFNVR LQCIEREIIS PRHEKMKTGL
410 420 430 440 450
IDKTQEPFSV RNKPFFDICT SRKLLEGNFA KEVSHEMDGL IFQPTGKYKP
460 470 480 490 500
GRCDDILKWK PPSLNSVDFR LKITRMGGEG LLPQNVGLLY VGGYERPFAQ
510 520 530 540 550
IKVTKELKQY DNKIIECKFE NNSWVFMRQR TDKSFPNAYN TAMAVCNSIS
560 570 580 590
NPVTKEMLFE FIDRCTAASQ GQKRKHHLDP DTELMPPPPP KRPRPLT
Length:597
Mass (Da):68,557
Last modified:August 1, 1998 - v1
Checksum:i51CEEC1B190603DE
GO
Isoform 2 (identifier: O60942-2) [UniParc]FASTAAdd to basket
Also known as: HCE1A

The sequence of this isoform differs from the canonical sequence as follows:
     424-446: Missing.

Show »
Length:574
Mass (Da):66,042
Checksum:iCFE64E51F6F88232
GO
Isoform 3 (identifier: O60942-3) [UniParc]FASTAAdd to basket
Also known as: HCE1B

The sequence of this isoform differs from the canonical sequence as follows:
     424-446: Missing.
     481-597: Missing.

Show »
Length:457
Mass (Da):52,533
Checksum:i4F3B52CA4F6CC56B
GO
Isoform 4 (identifier: O60942-4) [UniParc]FASTAAdd to basket
Also known as: HCAP1B

The sequence of this isoform differs from the canonical sequence as follows:
     504-597: TKELKQYDNK...PPPKRPRPLT → CLFIFSVLFL...KISCSSTLGG

Show »
Length:541
Mass (Da):61,705
Checksum:iDF795898785EC2D4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti30M → I in AAB91559 (PubMed:9371772).Curated1
Sequence conflicti212P → T in AAH19954 (PubMed:15489334).Curated1
Sequence conflicti484Q → P in AAB91559 (PubMed:9371772).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_046481594R → H1 PublicationCorresponds to variant dbSNP:rs17856595Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_003202424 – 446Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST23
Alternative sequenceiVSP_003203481 – 597Missing in isoform 3. 1 PublicationAdd BLAST117
Alternative sequenceiVSP_003204504 – 597TKELK…PRPLT → CLFIFSVLFLDVLLSGIHQN LANNNQHIKISCSSTLGG in isoform 4. 1 PublicationAdd BLAST94

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025654 mRNA Translation: AAB91559.1
AB009022 mRNA Translation: BAA25894.1
AB009023 mRNA Translation: BAA25895.1
AB009024 mRNA Translation: BAA25896.1
AB012142 mRNA Translation: BAA25198.1
AB012143 mRNA Translation: BAA25199.1
AL079342 Genomic DNA No translation available.
AL096868 Genomic DNA No translation available.
AL133408 Genomic DNA No translation available.
AL445530 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW48566.1
CH471051 Genomic DNA Translation: EAW48567.1
CH471051 Genomic DNA Translation: EAW48569.1
CH471051 Genomic DNA Translation: EAW48570.1
BC019954 mRNA Translation: AAH19954.1
CCDSiCCDS5017.1 [O60942-1]
CCDS69153.1 [O60942-2]
PIRiJC5936
JC5937
RefSeqiNP_001273355.1, NM_001286426.1 [O60942-2]
NP_001273357.1, NM_001286428.1
NP_003791.3, NM_003800.4 [O60942-1]
UniGeneiHs.567378
Hs.627135

Genome annotation databases

EnsembliENST00000369475; ENSP00000358487; ENSG00000111880 [O60942-2]
ENST00000369485; ENSP00000358497; ENSG00000111880 [O60942-1]
ENST00000538899; ENSP00000442609; ENSG00000111880 [O60942-3]
GeneIDi8732
KEGGihsa:8732
UCSCiuc003pmr.4 human [O60942-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF025654 mRNA Translation: AAB91559.1
AB009022 mRNA Translation: BAA25894.1
AB009023 mRNA Translation: BAA25895.1
AB009024 mRNA Translation: BAA25896.1
AB012142 mRNA Translation: BAA25198.1
AB012143 mRNA Translation: BAA25199.1
AL079342 Genomic DNA No translation available.
AL096868 Genomic DNA No translation available.
AL133408 Genomic DNA No translation available.
AL445530 Genomic DNA No translation available.
CH471051 Genomic DNA Translation: EAW48566.1
CH471051 Genomic DNA Translation: EAW48567.1
CH471051 Genomic DNA Translation: EAW48569.1
CH471051 Genomic DNA Translation: EAW48570.1
BC019954 mRNA Translation: AAH19954.1
CCDSiCCDS5017.1 [O60942-1]
CCDS69153.1 [O60942-2]
PIRiJC5936
JC5937
RefSeqiNP_001273355.1, NM_001286426.1 [O60942-2]
NP_001273357.1, NM_001286428.1
NP_003791.3, NM_003800.4 [O60942-1]
UniGeneiHs.567378
Hs.627135

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2C46X-ray1.60A/B/C/D1-219[»]
3S24X-ray3.01A/B/C/D/E/F/G229-567[»]
ProteinModelPortaliO60942
SMRiO60942
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114270, 55 interactors
DIPiDIP-61014N
IntActiO60942, 6 interactors
MINTiO60942
STRINGi9606.ENSP00000358497

Chemistry databases

DrugBankiDB02325 Isopropyl Alcohol

PTM databases

DEPODiO60942
iPTMnetiO60942
PhosphoSitePlusiO60942

Polymorphism and mutation databases

BioMutaiRNGTT

Proteomic databases

EPDiO60942
MaxQBiO60942
PaxDbiO60942
PeptideAtlasiO60942
PRIDEiO60942
ProteomicsDBi49688
49689 [O60942-2]
49690 [O60942-3]
49691 [O60942-4]

Protocols and materials databases

DNASUi8732
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000369475; ENSP00000358487; ENSG00000111880 [O60942-2]
ENST00000369485; ENSP00000358497; ENSG00000111880 [O60942-1]
ENST00000538899; ENSP00000442609; ENSG00000111880 [O60942-3]
GeneIDi8732
KEGGihsa:8732
UCSCiuc003pmr.4 human [O60942-1]

Organism-specific databases

CTDi8732
DisGeNETi8732
EuPathDBiHostDB:ENSG00000111880.15
GeneCardsiRNGTT
HGNCiHGNC:10073 RNGTT
HPAiHPA003750
MIMi603512 gene
neXtProtiNX_O60942
OpenTargetsiENSG00000111880
PharmGKBiPA34446
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2386 Eukaryota
COG5226 LUCA
GeneTreeiENSGT00530000063473
HOVERGENiHBG006332
InParanoidiO60942
KOiK13917
OMAiPELPEWC
OrthoDBiEOG091G063D
PhylomeDBiO60942
TreeFamiTF314914

Enzyme and pathway databases

BioCyciMetaCyc:HS03479-MONOMER
BRENDAi2.7.7.50 2681
ReactomeiR-HSA-167160 RNA Pol II CTD phosphorylation and interaction with CE during HIV infection
R-HSA-72086 mRNA Capping
R-HSA-77075 RNA Pol II CTD phosphorylation and interaction with CE

Miscellaneous databases

ChiTaRSiRNGTT human
EvolutionaryTraceiO60942
GeneWikiiRNGTT
GenomeRNAii8732
PROiPR:O60942
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000111880 Expressed in 221 organ(s), highest expression level in tonsil
CleanExiHS_RNGTT
GenevisibleiO60942 HS

Family and domain databases

Gene3Di3.90.190.10, 1 hit
InterProiView protein in InterPro
IPR000340 Dual-sp_phosphatase_cat-dom
IPR017074 mRNA_cap_enz_bifunc
IPR001339 mRNA_cap_enzyme
IPR013846 mRNA_cap_enzyme_C
IPR012340 NA-bd_OB-fold
IPR029021 Prot-tyrosine_phosphatase-like
IPR016130 Tyr_Pase_AS
IPR000387 TYR_PHOSPHATASE_dom
PfamiView protein in Pfam
PF00782 DSPc, 1 hit
PF03919 mRNA_cap_C, 1 hit
PF01331 mRNA_cap_enzyme, 1 hit
PIRSFiPIRSF036958 mRNA_capping_HCE, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52799 SSF52799, 1 hit
PROSITEiView protein in PROSITE
PS00383 TYR_PHOSPHATASE_1, 1 hit
PS50056 TYR_PHOSPHATASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMCE1_HUMAN
AccessioniPrimary (citable) accession number: O60942
Secondary accession number(s): E1P513
, E1P514, O43483, O60257, O60351, Q5TCW8, Q8WUM8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 7, 2018
This is version 179 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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