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Protein

Ribonuclease H1

Gene

RNASEH1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids (PubMed:10497183). Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II (PubMed:21700224).2 Publications

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.PROSITE-ProRule annotation

Cofactori

Mg2+Note: Binds 1 Mg2+ ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.

Activity regulationi

In the presence of magnesium, manganese is inhibitory.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi145Magnesium 1PROSITE-ProRule annotation1
Metal bindingi145Magnesium 2PROSITE-ProRule annotation1
Metal bindingi186Magnesium 1PROSITE-ProRule annotation1
Metal bindingi210Magnesium 1PROSITE-ProRule annotation1
Metal bindingi274Magnesium 2PROSITE-ProRule annotation1

GO - Molecular functioni

  • magnesium ion binding Source: InterPro
  • nucleic acid binding Source: ProtInc
  • ribonuclease activity Source: ProtInc
  • RNA binding Source: ProtInc
  • RNA-DNA hybrid ribonuclease activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease H1 (EC:3.1.26.4)
Short name:
RNase H1
Alternative name(s):
Ribonuclease H type II
Gene namesi
Name:RNASEH1
Synonyms:RNH1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000171865.9
HGNCiHGNC:18466 RNASEH1
MIMi604123 gene
neXtProtiNX_O60930

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Involvement in diseasei

Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal recessive 2 (PEOB2)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of progressive external ophthalmoplegia, a mitochondrial myopathy characterized by progressive paralysis of the levator palpebrae, orbicularis oculi, and extraocular muscles. PEOB2 patients manifest exercise intolerance, muscle weakness, and signs and symptoms of spinocerebellar ataxia, such as impaired gait and dysarthria. Some patients may have respiratory insufficiency.
See also OMIM:616479
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_074561142V → I in PEOB2; has partial residual endonuclease activity. 1 PublicationCorresponds to variant dbSNP:rs766294940EnsemblClinVar.1
Natural variantiVAR_074562185A → V in PEOB2; has partial residual endonuclease activity. 1 PublicationCorresponds to variant dbSNP:rs1057517675Ensembl.1

Keywords - Diseasei

Disease mutation, Primary mitochondrial disease, Progressive external ophthalmoplegia

Organism-specific databases

DisGeNETi246243
MalaCardsiRNASEH1
MIMi616479 phenotype
OpenTargetsiENSG00000171865
PharmGKBiPA38543

Chemistry databases

ChEMBLiCHEMBL5893

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001954331 – 286Ribonuclease H1Add BLAST286

Proteomic databases

EPDiO60930
MaxQBiO60930
PaxDbiO60930
PeptideAtlasiO60930
PRIDEiO60930
ProteomicsDBi49675

PTM databases

iPTMnetiO60930
PhosphoSitePlusiO60930

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSG00000171865 Expressed in 235 organ(s), highest expression level in secondary oocyte
CleanExiHS_RNASEH1
HS_RNH1
ExpressionAtlasiO60930 baseline and differential
GenevisibleiO60930 HS

Organism-specific databases

HPAiHPA043037

Interactioni

Subunit structurei

Monomer.Curated

Protein-protein interaction databases

BioGridi128882, 13 interactors
IntActiO60930, 8 interactors
MINTiO60930
STRINGi9606.ENSP00000313350

Chemistry databases

BindingDBiO60930

Structurei

Secondary structure

1286
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO60930
SMRiO60930
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60930

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini136 – 282RNase HPROSITE-ProRule annotationAdd BLAST147

Sequence similaritiesi

Belongs to the RNase H family.Curated

Phylogenomic databases

eggNOGiKOG3752 Eukaryota
COG0328 LUCA
GeneTreeiENSGT00390000003466
HOGENOMiHOG000040465
HOVERGENiHBG002135
InParanoidiO60930
KOiK03469
OMAiELWYGLY
OrthoDBiEOG091G0RCI
PhylomeDBiO60930
TreeFamiTF313356

Family and domain databases

Gene3Di3.30.420.10, 1 hit
3.40.970.10, 1 hit
InterProiView protein in InterPro
IPR009027 Ribosomal_L9/RNase_H1_N
IPR017067 RNase_H1_euk
IPR011320 RNase_H1_N
IPR037056 RNase_H1_N_sf
IPR012337 RNaseH-like_sf
IPR002156 RNaseH_domain
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF01693 Cauli_VI, 1 hit
PF00075 RNase_H, 1 hit
PIRSFiPIRSF036852 Ribonuclease_H1_euk, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
SSF55658 SSF55658, 1 hit
PROSITEiView protein in PROSITE
PS50879 RNASE_H, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.iShow all

O60930-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSWLLFLAHR VALAALPCRR GSRGFGMFYA VRRGRKTGVF LTWNECRAQV
60 70 80 90 100
DRFPAARFKK FATEDEAWAF VRKSASPEVS EGHENQHGQE SEAKASKRLR
110 120 130 140 150
EPLDGDGHES AEPYAKHMKP SVEPAPPVSR DTFSYMGDFV VVYTDGCCSS
160 170 180 190 200
NGRRRPRAGI GVYWGPGHPL NVGIRLPGRQ TNQRAEIHAA CKAIEQAKTQ
210 220 230 240 250
NINKLVLYTD SMFTINGITN WVQGWKKNGW KTSAGKEVIN KEDFVALERL
260 270 280
TQGMDIQWMH VPGHSGFIGN EEADRLAREG AKQSED
Length:286
Mass (Da):32,064
Last modified:April 27, 2001 - v2
Checksum:i400FE04E7E85CA6A
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F8WBP0F8WBP0_HUMAN
Ribonuclease H1
RNASEH1
43Annotation score:
F8WD93F8WD93_HUMAN
Ribonuclease H1
RNASEH1
43Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24G → R in AAV38476 (Ref. 5) Curated1
Sequence conflicti33R → K in CAG46619 (Ref. 4) Curated1
Sequence conflicti68W → R in AAV38476 (Ref. 5) Curated1
Sequence conflicti89Q → R in CAA11835 (PubMed:9894807).Curated1
Sequence conflicti136M → I in CAG46619 (Ref. 4) Curated1
Sequence conflicti223Q → R in CAA11835 (PubMed:9894807).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0234694L → F2 PublicationsCorresponds to variant dbSNP:rs1136545Ensembl.1
Natural variantiVAR_074561142V → I in PEOB2; has partial residual endonuclease activity. 1 PublicationCorresponds to variant dbSNP:rs766294940EnsemblClinVar.1
Natural variantiVAR_074562185A → V in PEOB2; has partial residual endonuclease activity. 1 PublicationCorresponds to variant dbSNP:rs1057517675Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048995 mRNA Translation: AAC78564.1
AF048994 mRNA Translation: AAC78563.1
AF039652 mRNA Translation: AAC09261.1
AJ224117 mRNA Translation: CAA11835.1
CR541820 mRNA Translation: CAG46619.1
BT019670 mRNA Translation: AAV38476.1
AK075490 mRNA Translation: BAG52156.1
AC108488 Genomic DNA Translation: AAX82026.1
CH471053 Genomic DNA Translation: EAX01061.1
BC002973 mRNA Translation: AAH02973.1
CCDSiCCDS1647.1
RefSeqiNP_002927.2, NM_002936.4
UniGeneiHs.568006

Genome annotation databases

EnsembliENST00000315212; ENSP00000313350; ENSG00000171865
GeneIDi246243
KEGGihsa:246243
UCSCiuc002qxt.5 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

Wikipedia

RNase H entry

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048995 mRNA Translation: AAC78564.1
AF048994 mRNA Translation: AAC78563.1
AF039652 mRNA Translation: AAC09261.1
AJ224117 mRNA Translation: CAA11835.1
CR541820 mRNA Translation: CAG46619.1
BT019670 mRNA Translation: AAV38476.1
AK075490 mRNA Translation: BAG52156.1
AC108488 Genomic DNA Translation: AAX82026.1
CH471053 Genomic DNA Translation: EAX01061.1
BC002973 mRNA Translation: AAH02973.1
CCDSiCCDS1647.1
RefSeqiNP_002927.2, NM_002936.4
UniGeneiHs.568006

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QK9X-ray2.55A136-286[»]
2QKBX-ray2.40A/B136-286[»]
2QKKX-ray3.20A/B/E/F/I/J/M/N/R/S/W136-286[»]
3BSUX-ray2.10A/B/C/F/G/H27-76[»]
ProteinModelPortaliO60930
SMRiO60930
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128882, 13 interactors
IntActiO60930, 8 interactors
MINTiO60930
STRINGi9606.ENSP00000313350

Chemistry databases

BindingDBiO60930
ChEMBLiCHEMBL5893

PTM databases

iPTMnetiO60930
PhosphoSitePlusiO60930

Proteomic databases

EPDiO60930
MaxQBiO60930
PaxDbiO60930
PeptideAtlasiO60930
PRIDEiO60930
ProteomicsDBi49675

Protocols and materials databases

DNASUi246243
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000315212; ENSP00000313350; ENSG00000171865
GeneIDi246243
KEGGihsa:246243
UCSCiuc002qxt.5 human

Organism-specific databases

CTDi246243
DisGeNETi246243
EuPathDBiHostDB:ENSG00000171865.9
GeneCardsiRNASEH1
H-InvDBiHIX0039191
HGNCiHGNC:18466 RNASEH1
HPAiHPA043037
MalaCardsiRNASEH1
MIMi604123 gene
616479 phenotype
neXtProtiNX_O60930
OpenTargetsiENSG00000171865
PharmGKBiPA38543
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3752 Eukaryota
COG0328 LUCA
GeneTreeiENSGT00390000003466
HOGENOMiHOG000040465
HOVERGENiHBG002135
InParanoidiO60930
KOiK03469
OMAiELWYGLY
OrthoDBiEOG091G0RCI
PhylomeDBiO60930
TreeFamiTF313356

Miscellaneous databases

ChiTaRSiRNASEH1 human
EvolutionaryTraceiO60930
GeneWikiiRNASEH1
GenomeRNAii246243
PROiPR:O60930
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000171865 Expressed in 235 organ(s), highest expression level in secondary oocyte
CleanExiHS_RNASEH1
HS_RNH1
ExpressionAtlasiO60930 baseline and differential
GenevisibleiO60930 HS

Family and domain databases

Gene3Di3.30.420.10, 1 hit
3.40.970.10, 1 hit
InterProiView protein in InterPro
IPR009027 Ribosomal_L9/RNase_H1_N
IPR017067 RNase_H1_euk
IPR011320 RNase_H1_N
IPR037056 RNase_H1_N_sf
IPR012337 RNaseH-like_sf
IPR002156 RNaseH_domain
IPR036397 RNaseH_sf
PfamiView protein in Pfam
PF01693 Cauli_VI, 1 hit
PF00075 RNase_H, 1 hit
PIRSFiPIRSF036852 Ribonuclease_H1_euk, 1 hit
SUPFAMiSSF53098 SSF53098, 1 hit
SSF55658 SSF55658, 1 hit
PROSITEiView protein in PROSITE
PS50879 RNASE_H, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRNH1_HUMAN
AccessioniPrimary (citable) accession number: O60930
Secondary accession number(s): B3KQU4
, O60523, O60857, Q57Z93, Q5U0C1, Q6FHD4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: September 12, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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