UniProtKB - O60911 (CATL2_HUMAN)
Protein
Cathepsin L2
Gene
CTSV
Organism
Homo sapiens (Human)
Status
Functioni
Cysteine protease. May have an important role in corneal physiology.2 Publications
Catalytic activityi
- The recombinant enzyme hydrolyzes proteins (serum albumin, collagen) and synthetic substrates (Z-Phe-Arg-NHMec > Z-Leu-Arg-NHMec > Z-Val-Arg-NHMec).1 Publication EC:3.4.22.43
Activity regulationi
Inhibited by CST6.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 138 | 1 Publication | 1 | |
Active sitei | 277 | 1 Publication | 1 | |
Active sitei | 301 | 1 Publication | 1 |
GO - Molecular functioni
- cysteine-type endopeptidase activity Source: GO_Central
- cysteine-type peptidase activity Source: ProtInc
GO - Biological processi
- antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
- extracellular matrix disassembly Source: Reactome
- immune response Source: GO_Central
- proteolysis involved in cellular protein catabolic process Source: GO_Central
- regulation of keratinocyte differentiation Source: Reactome
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Enzyme and pathway databases
BRENDAi | 3.4.22.43, 2681 |
PathwayCommonsi | O60911 |
Reactomei | R-HSA-1236977, Endosomal/Vacuolar pathway R-HSA-1474228, Degradation of the extracellular matrix R-HSA-1592389, Activation of Matrix Metalloproteinases R-HSA-1679131, Trafficking and processing of endosomal TLR R-HSA-2022090, Assembly of collagen fibrils and other multimeric structures R-HSA-2132295, MHC class II antigen presentation R-HSA-8939242, RUNX1 regulates transcription of genes involved in differentiation of keratinocytes |
SABIO-RKi | O60911 |
Protein family/group databases
MEROPSi | I29.010 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:CTSV Synonyms:CATL2, CTSL2, CTSU ORF Names:UNQ268/PRO305 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2538, CTSV |
MIMi | 603308, gene |
neXtProti | NX_O60911 |
VEuPathDBi | HostDB:ENSG00000136943.10 |
Subcellular locationi
Lysosome
- Lysosome Curated
Extracellular region or secreted
- extracellular region Source: Reactome
- extracellular space Source: GO_Central
Lysosome
- lysosomal lumen Source: Reactome
- lysosome Source: GO_Central
Keywords - Cellular componenti
LysosomePathology & Biotechi
Organism-specific databases
DisGeNETi | 1515 |
OpenTargetsi | ENSG00000136943 |
PharmGKBi | PA27036 |
Miscellaneous databases
Pharosi | O60911, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3272 |
DrugBanki | DB02869, 3-amino-5-phenylpentane DB04451, 4-Methylpiperazin-1-Yl Carbonyl Group |
DrugCentrali | O60911 |
GuidetoPHARMACOLOGYi | 2352 |
Genetic variation databases
BioMutai | CTSV |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 17 | Sequence analysisAdd BLAST | 17 | |
PropeptideiPRO_0000026277 | 18 – 113 | Activation peptideAdd BLAST | 96 | |
ChainiPRO_0000026278 | 114 – 334 | Cathepsin L2Add BLAST | 221 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 135 ↔ 178 | Combined sources1 Publication | ||
Disulfide bondi | 169 ↔ 211 | Combined sources1 Publication | ||
Glycosylationi | 221 | N-linked (GlcNAc...) asparagine1 Publication | 1 | |
Disulfide bondi | 270 ↔ 323 | Combined sources1 Publication | ||
Glycosylationi | 292 | N-linked (GlcNAc...) asparagine1 Publication | 1 |
Keywords - PTMi
Disulfide bond, Glycoprotein, ZymogenProteomic databases
EPDi | O60911 |
jPOSTi | O60911 |
MassIVEi | O60911 |
MaxQBi | O60911 |
PaxDbi | O60911 |
PeptideAtlasi | O60911 |
PRIDEi | O60911 |
ProteomicsDBi | 49669 |
PTM databases
GlyGeni | O60911, 2 sites |
iPTMneti | O60911 |
PhosphoSitePlusi | O60911 |
Expressioni
Tissue specificityi
Predominantly expressed in the thymus and testis. Also expressed in corneal epithelium, and to a lesser extent in conjunctival epithelium and skin.3 Publications
Gene expression databases
Bgeei | ENSG00000136943, Expressed in hair follicle and 193 other tissues |
ExpressionAtlasi | O60911, baseline and differential |
Genevisiblei | O60911, HS |
Organism-specific databases
HPAi | ENSG00000136943, Tissue enriched (lymphoid) |
Interactioni
Protein-protein interaction databases
BioGRIDi | 107895, 46 interactors |
IntActi | O60911, 34 interactors |
MINTi | O60911 |
STRINGi | 9606.ENSP00000445052 |
Chemistry databases
BindingDBi | O60911 |
Miscellaneous databases
RNActi | O60911, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O60911 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O60911 |
Family & Domainsi
Sequence similaritiesi
Belongs to the peptidase C1 family.PROSITE-ProRule annotation
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG1543, Eukaryota |
GeneTreei | ENSGT00940000154367 |
HOGENOMi | CLU_012184_1_2_1 |
InParanoidi | O60911 |
OMAi | CSHPQGN |
OrthoDBi | 1275401at2759 |
PhylomeDBi | O60911 |
TreeFami | TF313739 |
Family and domain databases
CDDi | cd02248, Peptidase_C1A, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR025661, Pept_asp_AS IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR039417, Peptidase_C1A_papain-like IPR013201, Prot_inhib_I29 |
Pfami | View protein in Pfam PF08246, Inhibitor_I29, 1 hit PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00848, Inhibitor_I29, 1 hit SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00640, THIOL_PROTEASE_ASN, 1 hit PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
O60911-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNLSLVLAAF CLGIASAVPK FDQNLDTKWY QWKATHRRLY GANEEGWRRA
60 70 80 90 100
VWEKNMKMIE LHNGEYSQGK HGFTMAMNAF GDMTNEEFRQ MMGCFRNQKF
110 120 130 140 150
RKGKVFREPL FLDLPKSVDW RKKGYVTPVK NQKQCGSCWA FSATGALEGQ
160 170 180 190 200
MFRKTGKLVS LSEQNLVDCS RPQGNQGCNG GFMARAFQYV KENGGLDSEE
210 220 230 240 250
SYPYVAVDEI CKYRPENSVA NDTGFTVVAP GKEKALMKAV ATVGPISVAM
260 270 280 290 300
DAGHSSFQFY KSGIYFEPDC SSKNLDHGVL VVGYGFEGAN SNNSKYWLVK
310 320 330
NSWGPEWGSN GYVKIAKDKN NHCGIATAAS YPNV
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 81 | G → P in CAA75029 (PubMed:9563472).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y14734 mRNA Translation: CAA75029.1 AB001928 mRNA Translation: BAA25909.1 AF070448 mRNA Translation: AAC23598.1 AB019534 Genomic DNA Translation: BAA34365.1 AY358641 mRNA Translation: AAQ89004.1 AL445670 Genomic DNA No translation available. BC023504 mRNA Translation: AAH23504.1 BC110512 mRNA Translation: AAI10513.1 |
CCDSi | CCDS6723.1 |
RefSeqi | NP_001188504.1, NM_001201575.1 NP_001324.2, NM_001333.3 |
Genome annotation databases
Ensembli | ENST00000259470; ENSP00000259470; ENSG00000136943 ENST00000538255; ENSP00000445052; ENSG00000136943 |
GeneIDi | 1515 |
KEGGi | hsa:1515 |
UCSCi | uc004awt.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y14734 mRNA Translation: CAA75029.1 AB001928 mRNA Translation: BAA25909.1 AF070448 mRNA Translation: AAC23598.1 AB019534 Genomic DNA Translation: BAA34365.1 AY358641 mRNA Translation: AAQ89004.1 AL445670 Genomic DNA No translation available. BC023504 mRNA Translation: AAH23504.1 BC110512 mRNA Translation: AAI10513.1 |
CCDSi | CCDS6723.1 |
RefSeqi | NP_001188504.1, NM_001201575.1 NP_001324.2, NM_001333.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1FH0 | X-ray | 1.60 | A/B | 114-334 | [»] | |
3H6S | X-ray | 2.22 | A/B/C/D | 114-334 | [»] | |
3KFQ | X-ray | 1.99 | A/B | 114-334 | [»] | |
SMRi | O60911 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 107895, 46 interactors |
IntActi | O60911, 34 interactors |
MINTi | O60911 |
STRINGi | 9606.ENSP00000445052 |
Chemistry databases
BindingDBi | O60911 |
ChEMBLi | CHEMBL3272 |
DrugBanki | DB02869, 3-amino-5-phenylpentane DB04451, 4-Methylpiperazin-1-Yl Carbonyl Group |
DrugCentrali | O60911 |
GuidetoPHARMACOLOGYi | 2352 |
Protein family/group databases
MEROPSi | I29.010 |
PTM databases
GlyGeni | O60911, 2 sites |
iPTMneti | O60911 |
PhosphoSitePlusi | O60911 |
Genetic variation databases
BioMutai | CTSV |
Proteomic databases
EPDi | O60911 |
jPOSTi | O60911 |
MassIVEi | O60911 |
MaxQBi | O60911 |
PaxDbi | O60911 |
PeptideAtlasi | O60911 |
PRIDEi | O60911 |
ProteomicsDBi | 49669 |
Protocols and materials databases
Antibodypediai | 28731, 368 antibodies |
Genome annotation databases
Ensembli | ENST00000259470; ENSP00000259470; ENSG00000136943 ENST00000538255; ENSP00000445052; ENSG00000136943 |
GeneIDi | 1515 |
KEGGi | hsa:1515 |
UCSCi | uc004awt.4, human |
Organism-specific databases
CTDi | 1515 |
DisGeNETi | 1515 |
GeneCardsi | CTSV |
HGNCi | HGNC:2538, CTSV |
HPAi | ENSG00000136943, Tissue enriched (lymphoid) |
MIMi | 603308, gene |
neXtProti | NX_O60911 |
OpenTargetsi | ENSG00000136943 |
PharmGKBi | PA27036 |
VEuPathDBi | HostDB:ENSG00000136943.10 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1543, Eukaryota |
GeneTreei | ENSGT00940000154367 |
HOGENOMi | CLU_012184_1_2_1 |
InParanoidi | O60911 |
OMAi | CSHPQGN |
OrthoDBi | 1275401at2759 |
PhylomeDBi | O60911 |
TreeFami | TF313739 |
Enzyme and pathway databases
BRENDAi | 3.4.22.43, 2681 |
PathwayCommonsi | O60911 |
Reactomei | R-HSA-1236977, Endosomal/Vacuolar pathway R-HSA-1474228, Degradation of the extracellular matrix R-HSA-1592389, Activation of Matrix Metalloproteinases R-HSA-1679131, Trafficking and processing of endosomal TLR R-HSA-2022090, Assembly of collagen fibrils and other multimeric structures R-HSA-2132295, MHC class II antigen presentation R-HSA-8939242, RUNX1 regulates transcription of genes involved in differentiation of keratinocytes |
SABIO-RKi | O60911 |
Miscellaneous databases
BioGRID-ORCSi | 1515, 5 hits in 973 CRISPR screens |
ChiTaRSi | CTSV, human |
EvolutionaryTracei | O60911 |
GeneWikii | Cathepsin_L2 |
GenomeRNAii | 1515 |
Pharosi | O60911, Tchem |
PROi | PR:O60911 |
RNActi | O60911, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000136943, Expressed in hair follicle and 193 other tissues |
ExpressionAtlasi | O60911, baseline and differential |
Genevisiblei | O60911, HS |
Family and domain databases
CDDi | cd02248, Peptidase_C1A, 1 hit |
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR025661, Pept_asp_AS IPR000169, Pept_cys_AS IPR025660, Pept_his_AS IPR000668, Peptidase_C1A_C IPR039417, Peptidase_C1A_papain-like IPR013201, Prot_inhib_I29 |
Pfami | View protein in Pfam PF08246, Inhibitor_I29, 1 hit PF00112, Peptidase_C1, 1 hit |
PRINTSi | PR00705, PAPAIN |
SMARTi | View protein in SMART SM00848, Inhibitor_I29, 1 hit SM00645, Pept_C1, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS00640, THIOL_PROTEASE_ASN, 1 hit PS00139, THIOL_PROTEASE_CYS, 1 hit PS00639, THIOL_PROTEASE_HIS, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CATL2_HUMAN | |
Accessioni | O60911Primary (citable) accession number: O60911 Secondary accession number(s): O60233, Q2TB86, Q5T1U0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 15, 1998 |
Last sequence update: | December 1, 2000 | |
Last modified: | April 7, 2021 | |
This is version 198 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families