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UniProtKB - O60885 (BRD4_HUMAN)

Entry version 199 (02 Jun 2021)
Sequence version 2 (31 Jan 2002)
Previous versions | rss
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Protein

Bromodomain-containing protein 4

Gene

BRD4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure (PubMed:23589332, PubMed:23317504, PubMed:22334664).

During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Also recruits P-TEFb complex to distal enhancers, so called anti-pause enhancers in collaboration with JMJD6. BRD4 and JMJD6 are required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II (PubMed:23589332, PubMed:19596240, PubMed:16109377, PubMed:16109376, PubMed:24360279).

Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II (PubMed:23086925).

According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028).

In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B (PubMed:19103749).

Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters (PubMed:23317504).

10 Publications

Acts as a chromatin insulator in the DNA damage response pathway. Inhibits DNA damage response signaling by recruiting the condensin-2 complex to acetylated histones, leading to chromatin structure remodeling, insulating the region from DNA damage response by limiting spreading of histone H2AX/H2A.x phosphorylation.

1 Publication

Miscellaneous

Some specific inhibitors of BRD4 that prevent binding to acetylated histones by binding Asn-140 and Asn-433 are promising therapeutic molecules for the treatment of leukemias. JQ1, a thieno-triazolo-1,4-diazepine derivative, and I-BET, a benzodiazepine derivative, have been tested on tumors with success (PubMed:20871596, PubMed:21068722, PubMed:21964340). Treatment with GSK1210151A (I-BET151, a I-BET derivative) has strong effets on mixed lineage leukemia and promotes myeloid differentiation and leukemia stem-cell depletion (PubMed:21964340).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei140Acetylated histone binding1 Publication1
Sitei433Acetylated histone binding1 Publication1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionChromatin regulator
Biological processDNA damage, Host-virus interaction, Transcription, Transcription regulation

Enzyme and pathway databases

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		O60885

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-9679191, Potential therapeutics for SARS

SIGNOR Signaling Network Open Resource

More...SIGNORi		O60885

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Bromodomain-containing protein 4
Alternative name(s):
Protein HUNK1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:BRD4
Synonyms:HUNK1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 19

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:13575, BRD4

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		608749, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_O60885

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000141867.17

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Chromosome, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section provides information on the disease(s) associated with genetic variations in a given protein. The information is extracted from the scientific literature and diseases that are also described in the <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim">OMIM</a> database are represented with a <a href="http://www.uniprot.org/diseases">controlled vocabulary</a> in the following way:<p><a href='/help/involvement_in_disease' target='_top'>More...</a></p>Involvement in diseasei

A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUTM1 which produces a BRD4-NUTM1 fusion protein.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi140N → A: Abolishes binding to acetylated histones. 2 Publications1
Mutagenesisi433N → A: Abolishes binding to acetylated histones. 1 Publication1
Mutagenesisi492 – 494SSS → ASA: Impaired phosphorylation by CK2 and binding to acetylated histones. 1 Publication3
Mutagenesisi498 – 500SST → AAA: Impaired phosphorylation by CK2 and binding to acetylated histones. 1 Publication3
Mutagenesisi503S → A: Impaired phosphorylation by CK2 and binding to acetylated histones. 1 Publication1
Mutagenesisi651 – 653EIE → AIA: Decreases interaction with JMJD6 and NSD3.No effect on interaction with histone 4 acetylated. 1 Publication3

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei719 – 720Breakpoint for translocation to form BDR4-NUTM1 fusion protein2

Organism-specific databases

DisGeNET

More...DisGeNETi		23476

GeneReviews a resource of expert-authored, peer-reviewed disease descriptions.

More...GeneReviewsi		BRD4

MalaCards human disease database

More...MalaCardsi		BRD4

Open Targets

More...OpenTargetsi		ENSG00000141867

Orphanet; a database dedicated to information on rare diseases and orphan drugs

More...Orphaneti		443167, NUT midline carcinoma

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA25416

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		O60885, Tchem

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL1163125

DrugCentral

More...DrugCentrali		O60885

IUPHAR/BPS Guide to PHARMACOLOGY

More...GuidetoPHARMACOLOGYi		1945

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		BRD4

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002111831 – 1362Bromodomain-containing protein 4Add BLAST1362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki99Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei470PhosphoserineCombined sources1
Modified residuei484Phosphoserine; by CK21 Publication1
Modified residuei488Phosphoserine; by CK21 Publication1
Modified residuei492Phosphoserine; by CK21 Publication1
Modified residuei494Phosphoserine; by CK21 Publication1
Modified residuei498Phosphoserine; by CK21 Publication1
Modified residuei499Phosphoserine; by CK21 Publication1
Modified residuei503Phosphoserine; by CK21 Publication1
Cross-linki585Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei601PhosphoserineCombined sources1
Cross-linki645Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki694Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki1050Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1111N6-acetyllysine; alternateCombined sources1
Cross-linki1111Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki1111Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei1117PhosphoserineCombined sources1
Modified residuei1126PhosphoserineCombined sources1
Cross-linki1197Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1201PhosphoserineCombined sources1
Modified residuei1204PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylation by CK2 disrupt the intramolecular binding between the bromo domain 2 and the NPS region and promotes binding between the NPS and the BID regions, leading to activate the protein and promote binding to acetylated histones. In absence of phosphorylation, BRD4 does not localize to p53/TP53 target gene promoters, phosphorylation promoting recruitment to p53/TP53 target promoters.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		O60885

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		O60885

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		O60885

MaxQB - The MaxQuant DataBase

More...MaxQBi		O60885

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		O60885

PeptideAtlas

More...PeptideAtlasi		O60885

PRoteomics IDEntifications database

More...PRIDEi		O60885

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		49651 [O60885-1]
49652 [O60885-2]
62139

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...GlyGeni		O60885, 2 sites, 1 O-linked glycan (2 sites)

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		O60885

MetOSite database of methionine sulfoxide sites

More...MetOSitei		O60885

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		O60885

SwissPalm database of S-palmitoylation events

More...SwissPalmi		O60885

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000141867, Expressed in amniotic fluid and 251 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		O60885, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		O60885, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000141867, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Binds acetylated histone H4 (PubMed:29176719).

Interacts with p53/TP53; the interaction is direct (PubMed:23317504).

Interacts (via CTD region) with CDK9 and CCNT1, acting as an associated component of P-TEFb complex (PubMed:16109376, PubMed:16109377, PubMed:23317504, PubMed:24360279).

Interacts with RELA (when acetylated at 'Lys-310')(PubMed:19103749).

Interacts (via NET domain) with NSD3, CHD4, BICRA and ATAD5 (PubMed:21555454,PubMed:29176719). The interaction with BICRA bridges BRD4 to the GBAF complex (PubMed:29374058, PubMed:16109376, PubMed:16109377, PubMed:19103749, PubMed:21555454, PubMed:23317504).

Interacts (via NET domain) with JMJD6 (via JmjC and N-terminal domains); the interaction is stronger in presence of ssRNA and recruits JMJD6 on distal enhancers (PubMed:24360279, PubMed:21555454, PubMed:29176719).

Interacts with NSD3 (PubMed:29176719). Isoform B: interacts with NCAPD3 and SMC2 (PubMed:23728299).

9 Publications

(Microbial infection) Interacts with bovine papillomavirus type 1 regulatory protein E2. This interactions may serve for the tethering of viral genomes to host mitotic chromosomes allowing successful partitioning of the viral genome during cell division.

1 Publication

(Microbial infection) Interacts with Epstein-Barr virus (EBV) protein EBNA1; this interaction facilitates transcriptional activation by EBNA1.

1 Publication

(Microbial infection) Interacts with human herpes virus-8 (HHV-8) protein LANA.

1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		117036, 1368 interactors

CORUM comprehensive resource of mammalian protein complexes

More...CORUMi		O60885

Database of interacting proteins

More...DIPi		DIP-39776N

Protein interaction database and analysis system

More...IntActi		O60885, 60 interactors

Molecular INTeraction database

More...MINTi		O60885

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000263377

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		O60885

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		O60885, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11362
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

Biological Magnetic Resonance Data Bank

More...BMRBi		O60885

Small Angle Scattering Biological Data Bank

More...SASBDBi		O60885

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		O60885

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		O60885

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini75 – 147Bromo 1PROSITE-ProRule annotationAdd BLAST73
Domaini368 – 440Bromo 2PROSITE-ProRule annotationAdd BLAST73
Domaini600 – 682NETPROSITE-ProRule annotationAdd BLAST83

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 58DisorderedSequence analysisAdd BLAST58
Regioni174 – 229DisorderedSequence analysisAdd BLAST56
Regioni242 – 352DisorderedSequence analysisAdd BLAST111
Regioni463 – 615DisorderedSequence analysisAdd BLAST153
Regioni484 – 503NPS regionAdd BLAST20
Regioni524 – 579BID regionAdd BLAST56
Regioni674 – 1100DisorderedSequence analysisAdd BLAST427
Regioni1047 – 1362C-terminal (CTD) regionAdd BLAST316
Regioni1116 – 1339DisorderedSequence analysisAdd BLAST224

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi20 – 39Polar residuesSequence analysisAdd BLAST20
Compositional biasi191 – 207Polar residuesSequence analysisAdd BLAST17
Compositional biasi208 – 224Pro residuesSequence analysisAdd BLAST17
Compositional biasi243 – 274Pro residuesSequence analysisAdd BLAST32
Compositional biasi316 – 340Basic and acidic residuesSequence analysisAdd BLAST25
Compositional biasi482 – 496Polar residuesSequence analysisAdd BLAST15
Compositional biasi503 – 517Basic and acidic residuesSequence analysisAdd BLAST15
Compositional biasi549 – 573Basic and acidic residuesSequence analysisAdd BLAST25
Compositional biasi597 – 615Basic and acidic residuesSequence analysisAdd BLAST19
Compositional biasi694 – 708Polar residuesSequence analysisAdd BLAST15
Compositional biasi729 – 745Basic residuesSequence analysisAdd BLAST17
Compositional biasi751 – 788Pro residuesSequence analysisAdd BLAST38
Compositional biasi830 – 847Pro residuesSequence analysisAdd BLAST18
Compositional biasi926 – 941Polar residuesSequence analysisAdd BLAST16
Compositional biasi947 – 999Pro residuesSequence analysisAdd BLAST53
Compositional biasi1012 – 1036Pro residuesSequence analysisAdd BLAST25
Compositional biasi1068 – 1094Polar residuesSequence analysisAdd BLAST27
Compositional biasi1176 – 1192Basic and acidic residuesSequence analysisAdd BLAST17
Compositional biasi1204 – 1224Polar residuesSequence analysisAdd BLAST21
Compositional biasi1225 – 1287Basic and acidic residuesSequence analysisAdd BLAST63
Compositional biasi1288 – 1323Polar residuesSequence analysisAdd BLAST36
Compositional biasi1324 – 1339Basic and acidic residuesSequence analysisAdd BLAST16

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The NET domain mediates interaction with a number of chromatin proteins involved in transcription regulation (NSD3, JMJD6, CHD4, GLTSCR1 and ATAD5).1 Publication
The C-terminal (CTD) region mediates interaction and recruitment of CDK9 and CCNT1 subunits of the P-TEFb complex (PubMed:16109376, PubMed:16109377). It is also required for maintenance of higher-order chromatin structure (PubMed:22334664).3 Publications
The 2 bromo domains mediate specific binding to acetylated histones via Asn-140 and Asn-433, respectively (PubMed:20871596). The exact combination of modified histone tails required to recruit BRD4 to target genes is still unclear. The first bromo domain has high affinity for acetylated histone H4 tail, whereas the second bromo domain recognizes multiply acetylated marks in histone H3 (PubMed:22464331). A number of specific inhibitors bind competitively to acetyl-lysine-binding residues Asn-140 and Asn-433, promoting removal from acetylated histones. Many of these inhibitors are benzodiazepine derivatives (PubMed:22137933, PubMed:22136404, PubMed:23517011, PubMed:23530754).6 Publications

Keywords - Domaini

Bromodomain, Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG1474, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000154549

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_001499_0_3_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		O60885

Identification of Orthologs from Complete Genome Data

More...OMAi		ERMRWAR

Database of Orthologous Groups

More...OrthoDBi		619848at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		O60885

TreeFam database of animal gene trees

More...TreeFami		TF317345

Family and domain databases

Conserved Domains Database

More...CDDi		cd05497, Bromo_Brdt_I_like, 1 hit
cd05498, Bromo_Brdt_II_like, 1 hit

Database of protein disorder

More...DisProti		DP01898

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		1.20.1270.220, 1 hit
1.20.920.10, 2 hits

Intrinsically Disordered proteins with Extensive Annotations and Literature

More...IDEALi		IID00111

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR031354, BRD4_CDT
IPR043508, Bromo_Brdt_I
IPR043509, Bromo_Brdt_II
IPR001487, Bromodomain
IPR036427, Bromodomain-like_sf
IPR018359, Bromodomain_CS
IPR027353, NET_dom
IPR038336, NET_sf

Pfam protein domain database

More...Pfami		View protein in Pfam
PF17035, BET, 1 hit
PF17105, BRD4_CDT, 1 hit
PF00439, Bromodomain, 2 hits

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00503, BROMODOMAIN

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00297, BROMO, 2 hits

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF47370, SSF47370, 2 hits

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00633, BROMODOMAIN_1, 1 hit
PS50014, BROMODOMAIN_2, 2 hits
PS51525, NET, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform A (identifier: O60885-1) [UniParc]FASTAAdd to basket
Also known as: Brd4L, Long

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSAESGPGTR LRNLPVMGDG LETSQMSTTQ AQAQPQPANA ASTNPPPPET 
        60         70         80         90        100
SNPNKPKRQT NQLQYLLRVV LKTLWKHQFA WPFQQPVDAV KLNLPDYYKI 
       110        120        130        140        150
IKTPMDMGTI KKRLENNYYW NAQECIQDFN TMFTNCYIYN KPGDDIVLMA 
       160        170        180        190        200
EALEKLFLQK INELPTEETE IMIVQAKGRG RGRKETGTAK PGVSTVPNTT 
       210        220        230        240        250
QASTPPQTQT PQPNPPPVQA TPHPFPAVTP DLIVQTPVMT VVPPQPLQTP 
       260        270        280        290        300
PPVPPQPQPP PAPAPQPVQS HPPIIAATPQ PVKTKKGVKR KADTTTPTTI 
       310        320        330        340        350
DPIHEPPSLP PEPKTTKLGQ RRESSRPVKP PKKDVPDSQQ HPAPEKSSKV 
       360        370        380        390        400
SEQLKCCSGI LKEMFAKKHA AYAWPFYKPV DVEALGLHDY CDIIKHPMDM 
       410        420        430        440        450
STIKSKLEAR EYRDAQEFGA DVRLMFSNCY KYNPPDHEVV AMARKLQDVF 
       460        470        480        490        500
EMRFAKMPDE PEEPVVAVSS PAVPPPTKVV APPSSSDSSS DSSSDSDSST 
       510        520        530        540        550
DDSEEERAQR LAELQEQLKA VHEQLAALSQ PQQNKPKKKE KDKKEKKKEK 
       560        570        580        590        600
HKRKEEVEEN KKSKAKEPPP KKTKKNNSSN SNVSKKEPAP MKSKPPPTYE 
       610        620        630        640        650
SEEEDKCKPM SYEEKRQLSL DINKLPGEKL GRVVHIIQSR EPSLKNSNPD 
       660        670        680        690        700
EIEIDFETLK PSTLRELERY VTSCLRKKRK PQAEKVDVIA GSSKMKGFSS 
       710        720        730        740        750
SESESSSESS SSDSEDSETE MAPKSKKKGH PGREQKKHHH HHHQQMQQAP 
       760        770        780        790        800
APVPQQPPPP PQQPPPPPPP QQQQQPPPPP PPPSMPQQAA PAMKSSPPPF 
       810        820        830        840        850
IATQVPVLEP QLPGSVFDPI GHFTQPILHL PQPELPPHLP QPPEHSTPPH 
       860        870        880        890        900
LNQHAVVSPP ALHNALPQQP SRPSNRAAAL PPKPARPPAV SPALTQTPLL 
       910        920        930        940        950
PQPPMAQPPQ VLLEDEEPPA PPLTSMQMQL YLQQLQKVQP PTPLLPSVKV 
       960        970        980        990       1000
QSQPPPPLPP PPHPSVQQQL QQQPPPPPPP QPQPPPQQQH QPPPRPVHLQ 
      1010       1020       1030       1040       1050
PMQFSTHIQQ PPPPQGQQPP HPPPGQQPPP PQPAKPQQVI QHHHSPRHHK 
      1060       1070       1080       1090       1100
SDPYSTGHLR EAPSPLMIHS PQMSQFQSLT HQSPPQQNVQ PKKQELRAAS 
      1110       1120       1130       1140       1150
VVQPQPLVVV KEEKIHSPII RSEPFSPSLR PEPPKHPESI KAPVHLPQRP 
      1160       1170       1180       1190       1200
EMKPVDVGRP VIRPPEQNAP PPGAPDKDKQ KQEPKTPVAP KKDLKIKNMG 
      1210       1220       1230       1240       1250
SWASLVQKHP TTPSSTAKSS SDSFEQFRRA AREKEEREKA LKAQAEHAEK 
      1260       1270       1280       1290       1300
EKERLRQERM RSREDEDALE QARRAHEEAR RRQEQQQQQR QEQQQQQQQQ 
      1310       1320       1330       1340       1350
AAAVAAAATP QAQSSQPQSM LDQQRELARK REQERRRREA MAATIDMNFQ 
      1360 
SDLLSIFEEN LF
Length:1,362
Mass (Da):152,219
Last modified:January 31, 2002 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iD52EFE1CF9960907
GO
Isoform C (identifier: O60885-2) [UniParc]FASTAAdd to basket
Also known as: Brd4S, Short

The sequence of this isoform differs from the canonical sequence as follows:
     720-722: EMA → GPA
     723-1362: Missing.

Show »
Length:722
Mass (Da):80,463
Checksum:iFF040EE016B37F87
GO
Isoform B (identifier: O60885-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     720-1362: EMAPKSKKKG...LLSIFEENLF → AFCTSGDFVS...ECARCCVGCS

Note: Does not contain the C-terminal (CTD) region required to recruit the P-TEFb complex.Curated
Show »
Length:794
Mass (Da):88,289
Checksum:iACB01CC53AA4C431
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M0QZD9M0QZD9_HUMAN
Bromodomain-containing protein 4
BRD4
572Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0QYW0M0QYW0_HUMAN
Bromodomain containing 4, isoform C...
BRD4 hCG_2000917
146Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0R0H4M0R0H4_HUMAN
Bromodomain-containing protein 4
BRD4
105Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAC27978 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_04191937P → S1 PublicationCorresponds to variant dbSNP:rs35177876Ensembl.1
Natural variantiVAR_041920371A → G1 PublicationCorresponds to variant dbSNP:rs55805532Ensembl.1
Natural variantiVAR_041921563S → N1 PublicationCorresponds to variant dbSNP:rs55970906Ensembl.1
Natural variantiVAR_041922598T → S1 PublicationCorresponds to variant dbSNP:rs34362023Ensembl.1
Natural variantiVAR_041923669R → H1 PublicationCorresponds to variant dbSNP:rs35824241Ensembl.1
Natural variantiVAR_0484271097R → H. Corresponds to variant dbSNP:rs35676845Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_047671720 – 1362EMAPK…EENLF → AFCTSGDFVSPGPSPYHSHV QCGRFREMLRWFLVDVEQTA AGQPHRQSAAGPAITWAPAI AYPSPECARCCVGCS in isoform B. 1 PublicationAdd BLAST643
Alternative sequenceiVSP_010902720 – 722EMA → GPA in isoform C. 1 Publication3
Alternative sequenceiVSP_010903723 – 1362Missing in isoform C. 1 PublicationAdd BLAST640

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AF386649 mRNA Translation: AAL26987.1
Y12059 mRNA Translation: CAA72780.1
AC004798 Genomic DNA Translation: AAC27978.1 Different initiation.
AC003111 Genomic DNA No translation available.
AC005776 Genomic DNA No translation available.
CH471106 Genomic DNA Translation: EAW84470.1
BC035266 mRNA Translation: AAH35266.1
AY166680 mRNA Translation: AAO22237.1 Different termination.

The Consensus CDS (CCDS) project

More...CCDSi		CCDS12328.1 [O60885-1]
CCDS46004.1 [O60885-2]
CCDS82307.1 [O60885-3]

NCBI Reference Sequences

More...RefSeqi		NP_001317313.1, NM_001330384.1 [O60885-3]
NP_055114.1, NM_014299.2 [O60885-2]
NP_490597.1, NM_058243.2 [O60885-1]
XP_011526156.1, XM_011527854.1
XP_011526158.1, XM_011527856.2

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000263377; ENSP00000263377; ENSG00000141867 [O60885-1]
ENST00000360016; ENSP00000353112; ENSG00000141867 [O60885-3]
ENST00000371835; ENSP00000360901; ENSG00000141867 [O60885-2]
ENST00000679869; ENSP00000506350; ENSG00000141867 [O60885-1]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		23476

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:23476

UCSC genome browser

More...UCSCi		uc002nar.4, human [O60885-1]

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross%5Freferences%5Fsection">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF386649 mRNA Translation: AAL26987.1
Y12059 mRNA Translation: CAA72780.1
AC004798 Genomic DNA Translation: AAC27978.1 Different initiation.
AC003111 Genomic DNA No translation available.
AC005776 Genomic DNA No translation available.
CH471106 Genomic DNA Translation: EAW84470.1
BC035266 mRNA Translation: AAH35266.1
AY166680 mRNA Translation: AAO22237.1 Different termination.
CCDSiCCDS12328.1 [O60885-1]
CCDS46004.1 [O60885-2]
CCDS82307.1 [O60885-3]
RefSeqiNP_001317313.1, NM_001330384.1 [O60885-3]
NP_055114.1, NM_014299.2 [O60885-2]
NP_490597.1, NM_058243.2 [O60885-1]
XP_011526156.1, XM_011527854.1
XP_011526158.1, XM_011527856.2

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I8NNMR-A352-457[»]
2LSPNMR-B333-460[»]
2MJVNMR-B333-460[»]
2N3KNMR-A600-678[»]
2NCZNMR-A601-683[»]
2ND0NMR-A601-683[»]
2ND1NMR-A601-683[»]
2NNUX-ray1.59B1343-1362[»]
2OSSX-ray1.35A44-168[»]
2OUOX-ray1.89A333-460[»]
2YELX-ray1.65A44-168[»]
2YEMX-ray2.30A/B333-460[»]
3MXFX-ray1.60A42-168[»]
3P5OX-ray1.60A44-168[»]
3SVFX-ray1.98A44-168[»]
3SVGX-ray1.68A44-168[»]
3U5JX-ray1.60A44-168[»]
3U5KX-ray1.80A/B/C/D44-168[»]
3U5LX-ray1.39A44-168[»]
3UVWX-ray1.37A44-168[»]
3UVXX-ray1.91A44-168[»]
3UVYX-ray2.02A44-168[»]
3UW9X-ray2.30A/B/C/D44-168[»]
3ZYUX-ray1.50A/B44-168[»]
4A9LX-ray1.60A44-168[»]
4BJXX-ray1.59A44-168[»]
4BW1X-ray1.40A44-168[»]
4BW2X-ray1.92A44-168[»]
4BW3X-ray1.50A44-168[»]
4BW4X-ray1.67A44-168[»]
4C66X-ray1.87A44-167[»]
4C67X-ray1.55A44-168[»]
4CFKX-ray1.55A44-168[»]
4CFLX-ray1.32A44-168[»]
4CL9X-ray1.40A44-168[»]
4CLBX-ray1.60A44-168[»]
4DONX-ray1.52A44-166[»]
4E96X-ray1.92A44-168[»]
4F3IX-ray1.40A44-168[»]
4GPJX-ray1.60A44-168[»]
4HBVX-ray1.63A42-168[»]
4HBWX-ray1.69A42-168[»]
4HBXX-ray1.62A42-168[»]
4HBYX-ray1.59A44-168[»]
4HXKX-ray1.61A44-167[»]
4HXLX-ray1.52A44-167[»]
4HXMX-ray1.50A44-166[»]
4HXNX-ray1.49A44-167[»]
4HXOX-ray1.76A44-167[»]
4HXPX-ray1.73A44-166[»]
4HXRX-ray1.53A44-167[»]
4HXSX-ray1.43A44-166[»]
4IOOX-ray1.25A44-168[»]
4IOQX-ray1.50A44-168[»]
4IORX-ray1.40A44-168[»]
4J0RX-ray1.72A44-168[»]
4J0SX-ray1.84A44-168[»]
4J3IX-ray1.24A44-168[»]
4KV1X-ray1.50A/B41-168[»]
4KV4X-ray2.00A351-459[»]
4LR6X-ray1.29A42-168[»]
4LRGX-ray2.21A42-168[»]
4LYIX-ray1.30A44-168[»]
4LYSX-ray1.83A44-168[»]
4LYWX-ray1.95A44-168[»]
4LZRX-ray1.85A44-168[»]
4LZSX-ray2.20A44-168[»]
4MENX-ray1.81A44-168[»]
4MEOX-ray1.72A44-168[»]
4MEPX-ray1.85A44-168[»]
4MEQX-ray1.77A44-168[»]
4MR3X-ray1.68A44-168[»]
4MR4X-ray1.66A44-168[»]
4NQMX-ray1.58A44-168[»]
4NR8X-ray1.64A44-168[»]
4NUCX-ray1.40A44-168[»]
4NUDX-ray1.20A44-168[»]
4NUEX-ray1.30A44-168[»]
4O70X-ray1.55A/B44-168[»]
4O71X-ray1.36A/B44-168[»]
4O72X-ray1.40A44-168[»]
4O74X-ray1.45A/B44-168[»]
4O75X-ray1.55A44-168[»]
4O76X-ray1.70A/B/C/D44-168[»]
4O77X-ray2.00A/B44-168[»]
4O78X-ray1.34A44-168[»]
4O7AX-ray1.34A44-168[»]
4O7BX-ray1.50A44-168[»]
4O7CX-ray1.55A44-168[»]
4O7EX-ray1.85A/B44-168[»]
4O7FX-ray1.80A/B44-168[»]
4OGIX-ray1.73A/B44-168[»]
4OGJX-ray1.65A/B44-168[»]
4PCEX-ray1.29A44-168[»]
4PCIX-ray1.25A44-168[»]
4PS5X-ray1.40A/B44-168[»]
4QB3X-ray0.94A44-168[»]
4QR3X-ray1.37A44-166[»]
4QR4X-ray1.28A44-166[»]
4QR5X-ray1.41A44-166[»]
4QZSX-ray1.45A44-168[»]
4UIXX-ray1.58A/B/C44-168[»]
4UIYX-ray1.30A44-168[»]
4UIZX-ray1.19A44-168[»]
4UYDX-ray1.37A44-183[»]
4WHWX-ray1.34A44-168[»]
4WIVX-ray1.56A44-168[»]
4X2IX-ray1.20A42-166[»]
4XY9X-ray1.83A42-168[»]
4XYAX-ray2.05A42-168[»]
4YH3X-ray1.60A44-170[»]
4YH4X-ray1.33A44-170[»]
4Z1QX-ray1.40A/B42-167[»]
4Z1SX-ray1.06A/B42-166[»]
4Z93X-ray1.27A349-460[»]
4ZC9X-ray0.99A44-168[»]
4ZW1X-ray1.75A44-168[»]
5A5SX-ray1.36A44-168[»]
5A85X-ray1.72A44-168[»]
5ACYX-ray2.01A/B44-168[»]
5AD2X-ray2.01A/B44-168[»]
5AD3X-ray1.49A/B44-168[»]
5BT4X-ray1.50A/B/C44-168[»]
5CFWX-ray1.15A44-168[»]
5COIX-ray1.62A44-168[»]
5CP5X-ray1.79A44-168[»]
5CPEX-ray1.62A44-168[»]
5CQTX-ray1.60A44-168[»]
5CRMX-ray1.99A44-168[»]
5CRZX-ray2.12A44-168[»]
5CS8X-ray1.62A44-168[»]
5CTLX-ray2.51A44-168[»]
5CY9X-ray1.55A44-168[»]
5D0CX-ray1.49A44-168[»]
5D24X-ray1.65A43-168[»]
5D25X-ray1.70A43-168[»]
5D26X-ray1.82A42-168[»]
5D3HX-ray1.70A44-168[»]
5D3JX-ray1.70A43-168[»]
5D3LX-ray1.50A42-168[»]
5D3NX-ray2.15A43-168[»]
5D3PX-ray1.95A42-168[»]
5D3RX-ray2.20A42-168[»]
5D3SX-ray1.75A44-168[»]
5D3TX-ray1.93A42-168[»]
5DLXX-ray1.90A44-168[»]
5DLZX-ray1.70A44-168[»]
5DW2X-ray1.12A44-170[»]
5DX4X-ray2.30A44-168[»]
5E0RX-ray1.35A44-168[»]
5EGUX-ray2.21A/B/C/D44-168[»]
5EI4X-ray1.05A44-168[»]
5EISX-ray1.60A44-168[»]
5F5ZX-ray1.76A44-168[»]
5F60X-ray1.35A44-168[»]
5F61X-ray1.45A/B44-168[»]
5F62X-ray1.35A44-168[»]
5F63X-ray1.45A44-168[»]
5FBXX-ray1.85A44-168[»]
5H21X-ray1.59A44-167[»]
5HCLX-ray1.50A44-168[»]
5HLSX-ray2.18A42-168[»]
5HM0X-ray1.40A42-168[»]
5HQ5X-ray1.60A44-168[»]
5HQ6X-ray1.95A44-166[»]
5HQ7X-ray1.90A44-167[»]
5I80X-ray1.45A42-167[»]
5I88X-ray1.40A42-167[»]
5IGKX-ray1.70A44-168[»]
5JWMX-ray1.71A/B333-460[»]
5KDHX-ray1.50A44-168[»]
5KHMX-ray1.48A/B44-168[»]
5KJ0X-ray1.51A44-168[»]
5KU3X-ray1.14A42-167[»]
5LJ1X-ray1.90A42-168[»]
5LJ2X-ray1.19A42-168[»]
5LRQX-ray1.70A42-163[»]
5LUUX-ray1.61A44-168[»]
5M39X-ray1.38A/B42-168[»]
5M3AX-ray1.65A44-168[»]
5MKZX-ray1.62A44-168[»]
5MLIX-ray1.63A42-168[»]
5N2MX-ray1.54A44-168[»]
5NNCX-ray2.22A/B44-168[»]
5NNDX-ray1.82A/B44-168[»]
5NNEX-ray1.15A44-168[»]
5NNFX-ray1.15A44-168[»]
5NNGX-ray1.20A44-168[»]
5O97X-ray1.30A44-168[»]
5OVBX-ray1.95A/B44-168[»]
5OWMX-ray1.50A44-168[»]
5OWWX-ray1.50A/B/C/D44-168[»]
5T35X-ray2.70A/E333-460[»]
5TI2X-ray1.65A44-168[»]
5TI3X-ray1.70A44-168[»]
5TI4X-ray1.62A44-168[»]
5TI5X-ray1.83A44-168[»]
5TI6X-ray1.70A44-168[»]
5TI7X-ray1.65A44-168[»]
5U28X-ray1.80A44-180[»]
5U2CX-ray3.30A/B342-460[»]
5U2EX-ray1.99A/B42-180[»]
5U2FX-ray2.52A/B42-180[»]
5UEOX-ray1.85A/B352-457[»]
5UEPX-ray1.77A352-457[»]
5UEQX-ray1.70A352-457[»]
5UERX-ray1.87A352-457[»]
5UESX-ray1.62A352-457[»]
5UETX-ray2.29A352-457[»]
5UEUX-ray2.26A/B352-457[»]
5UEVX-ray1.94A352-457[»]
5UEXX-ray2.29A352-457[»]
5UEYX-ray2.41A352-457[»]
5UEZX-ray1.51A352-457[»]
5UF0X-ray1.35A352-457[»]
5ULAX-ray1.50A/B44-168[»]
5UOOX-ray1.69A333-460[»]
5UVSX-ray2.15A352-457[»]
5UVTX-ray1.67A352-457[»]
5UVUX-ray1.66A352-457[»]
5UVVX-ray1.99A/B352-457[»]
5UVWX-ray2.14A/B/C57-165[»]
5UVXX-ray1.53A/B352-457[»]
5UVYX-ray2.25A352-457[»]
5UVZX-ray1.63A352-457[»]
5V67X-ray1.78A44-168[»]
5VBOX-ray1.30A44-168[»]
5VBPX-ray1.83A/B44-168[»]
5VOMX-ray1.67A/B44-168[»]
5VZSX-ray1.71A/B42-168[»]
5W55X-ray1.35A42-168[»]
5WA5X-ray1.17A42-168[»]
5WMAX-ray1.40A44-168[»]
5WMDX-ray1.27A44-168[»]
5WMGX-ray1.19A44-168[»]
5WUUX-ray1.72A44-167[»]
5XHYX-ray1.98A44-166[»]
5XI2X-ray1.91A44-166[»]
5XI3X-ray1.67A44-166[»]
5XI4X-ray1.49A44-166[»]
5Y1YX-ray1.91A44-167[»]
5Y8CX-ray1.42A44-166[»]
5Y8WX-ray1.76A44-168[»]
5Y8YX-ray1.87A44-168[»]
5Y8ZX-ray1.84A44-168[»]
5Y93X-ray1.62A44-168[»]
5Y94X-ray2.00A44-168[»]
5YOUX-ray1.50A42-168[»]
5YOVX-ray1.45A42-168[»]
5YQXX-ray1.82A44-167[»]
5Z1RX-ray1.62A44-168[»]
5Z1SX-ray1.42A44-168[»]
5Z1TX-ray1.42A44-168[»]
5Z5TX-ray1.99A44-167[»]
5Z5UX-ray1.63A44-167[»]
5Z5VX-ray1.66A44-167[»]
5Z8GX-ray1.70A44-168[»]
5Z8RX-ray2.00A44-168[»]
5Z8ZX-ray1.80A44-168[»]
5Z90X-ray1.80A44-168[»]
5Z9CNMR-A53-168[»]
5Z9KX-ray1.89A44-168[»]
6AFRX-ray2.00A44-168[»]
6AJVX-ray1.45A42-168[»]
6AJWX-ray1.40A42-168[»]
6AJXX-ray1.89A42-168[»]
6AJYX-ray1.60A42-168[»]
6AJZOther1.30A42-168[»]
6BN7X-ray3.50C42-168[»]
6BN8X-ray3.99C42-168[»]
6BN9X-ray4.38C42-168[»]
6BNBX-ray6.34C42-168[»]
6BNHNMR-A601-683[»]
6BOYX-ray3.33C42-168[»]
6C7QX-ray1.51A333-460[»]
6C7RX-ray1.50A44-165[»]
6CD4X-ray1.23A42-168[»]
6CD5X-ray1.58A44-168[»]
6CISX-ray1.51A44-166[»]
6CIYX-ray1.68A44-168[»]
6CJ1X-ray1.53A44-166[»]
6CJ2X-ray1.47A42-166[»]
6CKRX-ray1.62A/B44-168[»]
6CKSX-ray1.72A44-168[»]
6CZUX-ray1.47A42-170[»]
6CZVX-ray1.88A42-170[»]
6DJCX-ray1.46A/B44-173[»]
6DL2X-ray1.47A44-168[»]
6DMJX-ray1.15A44-168[»]
6DMLX-ray1.50A44-168[»]
6DNEX-ray2.96A/B44-477[»]
6DUVX-ray1.80A/B347-458[»]
6E4AX-ray1.26A/B44-170[»]
6FFDX-ray1.83A347-463[»]
6FNXX-ray1.19A44-168[»]
6FO5X-ray0.95A44-168[»]
6FSYX-ray1.34A42-168[»]
6FT3X-ray1.28A42-168[»]
6FT4X-ray1.34A42-168[»]
6G0DX-ray1.31A42-168[»]
6G0EX-ray1.61A42-168[»]
6G0FX-ray1.62A42-168[»]
6G0GX-ray1.48A42-168[»]
6G0HX-ray1.91A42-168[»]
6G0OX-ray1.40A42-168[»]
6G0PX-ray1.30A42-168[»]
6G0QX-ray1.40A42-168[»]
6G0RX-ray1.25A42-168[»]
6G0SX-ray1.48A/B42-168[»]
6HDQX-ray1.70A44-168[»]
6HOVX-ray1.85A44-168[»]
6I7XX-ray1.20A44-168[»]
6I7YX-ray1.00A44-168[»]
6IN1X-ray1.50A42-168[»]
6JI3X-ray2.20A44-166[»]
6JI4X-ray1.60A44-166[»]
6JI5X-ray2.00A44-166[»]
6JJ3X-ray1.72A44-167[»]
6JJ5X-ray1.20A44-167[»]
6JJ6X-ray1.40A44-167[»]
6JJBX-ray1.51A44-167[»]
6KECX-ray1.35A44-168[»]
6KEDX-ray2.55A44-168[»]
6KEEX-ray2.12A44-168[»]
6KEFX-ray2.44A44-168[»]
6KEGX-ray2.23A44-168[»]
6KEHX-ray1.55A44-168[»]
6KEIX-ray1.45A44-168[»]
6KEJX-ray1.85A44-168[»]
6KEKX-ray1.55A44-168[»]
6KO2X-ray1.50A351-457[»]
6LG4X-ray1.85A44-167[»]
6LG5X-ray1.83A44-167[»]
6LG6X-ray1.98A44-167[»]
6LG7X-ray1.83A44-167[»]
6LG8X-ray1.58A44-167[»]
6LG9X-ray1.81A44-167[»]
6LIHX-ray1.62A44-166[»]
6LIMX-ray1.76A44-166[»]
6MAUX-ray2.11A44-170[»]
6MH1X-ray1.60A/B44-168[»]
6MH7X-ray1.74A/B44-168[»]
6MNLNMR-B333-460[»]
6P05X-ray1.54A44-168[»]
6PRTX-ray1.30A43-168[»]
6PS9X-ray1.21A44-168[»]
6PSBX-ray1.59A44-168[»]
6Q3YX-ray1.20A/B42-168[»]
6Q3ZX-ray2.00A/B44-168[»]
6RWJX-ray1.40A44-168[»]
6S25X-ray1.10A44-168[»]
6S4BX-ray1.60A44-168[»]
6S6KX-ray1.40A44-168[»]
6SA2X-ray1.50A44-168[»]
6SA3X-ray1.80A44-168[»]
6SAHX-ray1.50A44-168[»]
6SAJX-ray1.50A44-168[»]
6SB8X-ray1.50A44-168[»]
6SE4X-ray1.38A44-168[»]
6SISX-ray3.50A/E333-460[»]
6SWNX-ray1.28AAA44-168[»]
6SWQX-ray1.60AAA44-168[»]
6TPXX-ray1.48AAA44-168[»]
6TPYX-ray1.80AAA44-168[»]
6TPZX-ray1.30AAA44-168[»]
6U0DX-ray1.43A44-168[»]
6U6KX-ray1.70A42-168[»]
6U6LX-ray2.60A347-464[»]
6U72X-ray2.30A/B42-168[»]
6U74X-ray1.85A/B/C/D42-168[»]
6U8GX-ray2.60A/B/C/D42-168[»]
6U8IX-ray2.50A347-464[»]
6U8MX-ray1.95A/B42-168[»]
6ULSX-ray1.50A42-168[»]
6ULVX-ray2.20A/B/C/D42-168[»]
6UVJX-ray1.38A44-168[»]
6UVMX-ray1.51A44-168[»]
6UWUX-ray2.00A44-168[»]
6UWXX-ray1.31A44-168[»]
6V0UX-ray1.40A44-168[»]
6V1KX-ray1.75A44-168[»]
6V1LX-ray2.10A44-168[»]
6V1UX-ray1.73A44-168[»]
6VIWX-ray2.43A/B/C57-168[»]
6VIXX-ray2.12A/B/C/D352-457[»]
6VIZX-ray2.39A/B/C57-168[»]
6VUBX-ray1.50A44-168[»]
6VUCX-ray1.55A44-168[»]
6VUFX-ray1.59A/B44-168[»]
6VUJX-ray1.48A44-168[»]
6WGXX-ray1.53A/B44-168[»]
6WW8X-ray2.30A43-180[»]
6X7BX-ray1.95A/B44-176[»]
6X7CX-ray2.70A44-176[»]
6X7DX-ray2.50A/B44-176[»]
6XUZX-ray1.07A44-168[»]
6XV3X-ray1.47A/B/C/D44-168[»]
6XV7X-ray1.67A44-168[»]
6XVCX-ray1.10B44-168[»]
6YINX-ray1.53A44-168[»]
6YQNX-ray1.05A44-168[»]
6YQOX-ray1.07A44-168[»]
6YQPX-ray1.25A44-168[»]
6Z7GX-ray1.59AAA44-168[»]
6Z7LX-ray1.62AAA44-168[»]
6Z7MX-ray1.26AAA44-168[»]
6ZB3X-ray1.42AAA44-168[»]
6ZCIX-ray1.98A44-168[»]
6ZEDX-ray1.08AAA44-168[»]
6ZELX-ray1.12AAA44-168[»]
6ZF9X-ray1.20AAA44-168[»]
7A9UX-ray1.44AAA44-168[»]
7AJNX-ray1.48A44-168[»]
7KHHX-ray2.28D44-168[»]
7KHLX-ray1.29A/B44-168[»]
BMRBiO60885
SASBDBiO60885
SMRiO60885
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi117036, 1368 interactors
CORUMiO60885
DIPiDIP-39776N
IntActiO60885, 60 interactors
MINTiO60885
STRINGi9606.ENSP00000263377

Chemistry databases

BindingDBiO60885
ChEMBLiCHEMBL1163125
DrugCentraliO60885
GuidetoPHARMACOLOGYi1945

PTM databases

GlyGeniO60885, 2 sites, 1 O-linked glycan (2 sites)
iPTMnetiO60885
MetOSiteiO60885
PhosphoSitePlusiO60885
SwissPalmiO60885

Genetic variation databases

BioMutaiBRD4

Proteomic databases

EPDiO60885
jPOSTiO60885
MassIVEiO60885
MaxQBiO60885
PaxDbiO60885
PeptideAtlasiO60885
PRIDEiO60885
ProteomicsDBi49651 [O60885-1]
49652 [O60885-2]
62139

Protocols and materials databases

ABCD curated depository of sequenced antibodies

More...ABCDi		O60885, 2 sequenced antibodies

Antibodypedia a portal for validated antibodies

More...Antibodypediai		13956, 408 antibodies

The DNASU plasmid repository

More...DNASUi		23476

Genome annotation databases

EnsembliENST00000263377; ENSP00000263377; ENSG00000141867 [O60885-1]
ENST00000360016; ENSP00000353112; ENSG00000141867 [O60885-3]
ENST00000371835; ENSP00000360901; ENSG00000141867 [O60885-2]
ENST00000679869; ENSP00000506350; ENSG00000141867 [O60885-1]
GeneIDi23476
KEGGihsa:23476
UCSCiuc002nar.4, human [O60885-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		23476
DisGeNETi23476

GeneCards: human genes, protein and diseases

More...GeneCardsi		BRD4
GeneReviewsiBRD4
HGNCiHGNC:13575, BRD4
HPAiENSG00000141867, Low tissue specificity
MalaCardsiBRD4
MIMi608749, gene
neXtProtiNX_O60885
OpenTargetsiENSG00000141867
Orphaneti443167, NUT midline carcinoma
PharmGKBiPA25416
VEuPathDBiHostDB:ENSG00000141867.17

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG1474, Eukaryota
GeneTreeiENSGT00940000154549
HOGENOMiCLU_001499_0_3_1
InParanoidiO60885
OMAiERMRWAR
OrthoDBi619848at2759
PhylomeDBiO60885
TreeFamiTF317345

Enzyme and pathway databases

PathwayCommonsiO60885
ReactomeiR-HSA-9679191, Potential therapeutics for SARS
SIGNORiO60885

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		23476, 676 hits in 1026 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		BRD4, human
EvolutionaryTraceiO60885

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		BRD4

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		23476
PharosiO60885, Tchem

Protein Ontology

More...PROi		PR:O60885
RNActiO60885, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000141867, Expressed in amniotic fluid and 251 other tissues
ExpressionAtlasiO60885, baseline and differential
GenevisibleiO60885, HS

Family and domain databases

CDDicd05497, Bromo_Brdt_I_like, 1 hit
cd05498, Bromo_Brdt_II_like, 1 hit
DisProtiDP01898
Gene3Di1.20.1270.220, 1 hit
1.20.920.10, 2 hits
IDEALiIID00111
InterProiView protein in InterPro
IPR031354, BRD4_CDT
IPR043508, Bromo_Brdt_I
IPR043509, Bromo_Brdt_II
IPR001487, Bromodomain
IPR036427, Bromodomain-like_sf
IPR018359, Bromodomain_CS
IPR027353, NET_dom
IPR038336, NET_sf
PfamiView protein in Pfam
PF17035, BET, 1 hit
PF17105, BRD4_CDT, 1 hit
PF00439, Bromodomain, 2 hits
PRINTSiPR00503, BROMODOMAIN
SMARTiView protein in SMART
SM00297, BROMO, 2 hits
SUPFAMiSSF47370, SSF47370, 2 hits
PROSITEiView protein in PROSITE
PS00633, BROMODOMAIN_1, 1 hit
PS50014, BROMODOMAIN_2, 2 hits
PS51525, NET, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiBRD4_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O60885
Secondary accession number(s): O60433
, Q4G0X8, Q86YS8, Q96PD3
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 31, 2002
Last modified: June 2, 2021
This is version 199 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with genetic variants
    List of human entries with genetic variants
  3. Human variants curated from literature reports
    Index of human variants curated from literature reports
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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