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Entry version 188 (16 Oct 2019)
Sequence version 2 (31 Jan 2002)
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Protein

Bromodomain-containing protein 4

Gene

BRD4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Chromatin reader protein that recognizes and binds acetylated histones and plays a key role in transmission of epigenetic memory across cell divisions and transcription regulation. Remains associated with acetylated chromatin throughout the entire cell cycle and provides epigenetic memory for postmitotic G1 gene transcription by preserving acetylated chromatin status and maintaining high-order chromatin structure (PubMed:23589332, PubMed:23317504, PubMed:22334664). During interphase, plays a key role in regulating the transcription of signal-inducible genes by associating with the P-TEFb complex and recruiting it to promoters. Also recruits P-TEFb complex to distal enhancers, so called anti-pause enhancers in collaboration with JMJD6. BRD4 and JMJD6 are required to form the transcriptionally active P-TEFb complex by displacing negative regulators such as HEXIM1 and 7SKsnRNA complex from P-TEFb, thereby transforming it into an active form that can then phosphorylate the C-terminal domain (CTD) of RNA polymerase II (PubMed:23589332, PubMed:19596240, PubMed:16109377, PubMed:16109376, PubMed:24360279). Promotes phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II (PubMed:23086925). According to a report, directly acts as an atypical protein kinase and mediates phosphorylation of 'Ser-2' of the C-terminal domain (CTD) of RNA polymerase II; these data however need additional evidences in vivo (PubMed:22509028). In addition to acetylated histones, also recognizes and binds acetylated RELA, leading to further recruitment of the P-TEFb complex and subsequent activation of NF-kappa-B (PubMed:19103749). Also acts as a regulator of p53/TP53-mediated transcription: following phosphorylation by CK2, recruited to p53/TP53 specific target promoters (PubMed:23317504).10 Publications
Isoform B: Acts as a chromatin insulator in the DNA damage response pathway. Inhibits DNA damage response signaling by recruiting the condensin-2 complex to acetylated histones, leading to chromatin structure remodeling, insulating the region from DNA damage response by limiting spreading of histone H2AFX/H2A.x phosphorylation.1 Publication

Miscellaneous

Some specific inhibitors of BRD4 that prevent binding to acetylated histones by binding Asn-140 and Asn-433 are promising therapeutic molecules for the treatment of leukemias. JQ1, a thieno-triazolo-1,4-diazepine derivative, and I-BET, a benzodiazepine derivative, have been tested on tumors with success (PubMed:20871596, PubMed:21068722, PubMed:21964340). Treatment with GSK1210151A (I-BET151, a I-BET derivative) has strong effets on mixed lineage leukemia and promotes myeloid differentiation and leukemia stem-cell depletion (PubMed:21964340).3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei140Acetylated histones1 Publication1
Binding sitei140Inhibitor1
Binding sitei433Acetylated histones1 Publication1
Binding sitei433Inhibitor1

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionChromatin regulator
Biological processDNA damage, Host-virus interaction, Transcription, Transcription regulation

Enzyme and pathway databases

SIGNOR Signaling Network Open Resource

More...
SIGNORi
O60885

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Bromodomain-containing protein 4
Alternative name(s):
Protein HUNK1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:BRD4
Synonyms:HUNK1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 19

Organism-specific databases

Human Gene Nomenclature Database

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HGNCi
HGNC:13575 BRD4

Online Mendelian Inheritance in Man (OMIM)

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MIMi
608749 gene

neXtProt; the human protein knowledge platform

More...
neXtProti
NX_O60885

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the ‘Pathology and Biotech’ section provides information on the disease(s) associated with genetic variations in a given protein. The information is extracted from the scientific literature and diseases that are also described in the <a href="http://www.ncbi.nlm.nih.gov/sites/entrez?db=omim">OMIM</a> database are represented with a <a href="http://www.uniprot.org/diseases">controlled vocabulary</a> in the following way:<p><a href='/help/involvement_in_disease' target='_top'>More...</a></p>Involvement in diseasei

A chromosomal aberration involving BRD4 is found in a rare, aggressive, and lethal carcinoma arising in midline organs of young people. Translocation t(15;19)(q14;p13) with NUTM1 which produces a BRD4-NUTM1 fusion protein.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi140N → A: Abolishes binding to acetylated histones. 2 Publications1
Mutagenesisi433N → A: Abolishes binding to acetylated histones. 1 Publication1
Mutagenesisi492 – 494SSS → ASA: Impaired phosphorylation by CK2 and binding to acetylated histones. 1 Publication3
Mutagenesisi498 – 500SST → AAA: Impaired phosphorylation by CK2 and binding to acetylated histones. 1 Publication3
Mutagenesisi503S → A: Impaired phosphorylation by CK2 and binding to acetylated histones. 1 Publication1
Mutagenesisi651 – 653EIE → AIA: Decreases interaction with JMJD6 and NSD3.No effect on interaction with histone 4 acetylated. 1 Publication3

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections (‘Function’, ‘PTM / Processing’, ‘Pathology and Biotech’) according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei719 – 720Breakpoint for translocation to form BDR4-NUTM1 fusion protein2

Organism-specific databases

DisGeNET

More...
DisGeNETi
23476

MalaCards human disease database

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MalaCardsi
BRD4

Open Targets

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OpenTargetsi
ENSG00000141867

Orphanet; a database dedicated to information on rare diseases and orphan drugs

More...
Orphaneti
443167 NUT midline carcinoma

The Pharmacogenetics and Pharmacogenomics Knowledge Base

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PharmGKBi
PA25416

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

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Pharosi
O60885

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

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ChEMBLi
CHEMBL1163125

DrugCentral

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DrugCentrali
O60885

IUPHAR/BPS Guide to PHARMACOLOGY

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GuidetoPHARMACOLOGYi
1945

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

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BioMutai
BRD4

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00002111831 – 1362Bromodomain-containing protein 4Add BLAST1362

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki99Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei470PhosphoserineCombined sources1
Modified residuei484Phosphoserine; by CK21 Publication1
Modified residuei488Phosphoserine; by CK21 Publication1
Modified residuei492Phosphoserine; by CK21 Publication1
Modified residuei494Phosphoserine; by CK21 Publication1
Modified residuei498Phosphoserine; by CK21 Publication1
Modified residuei499Phosphoserine; by CK21 Publication1
Modified residuei503Phosphoserine; by CK21 Publication1
Cross-linki585Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei601PhosphoserineCombined sources1
Cross-linki645Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki694Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki1050Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1111N6-acetyllysine; alternateCombined sources1
Cross-linki1111Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki1111Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei1117PhosphoserineCombined sources1
Modified residuei1126PhosphoserineCombined sources1
Cross-linki1197Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei1201PhosphoserineCombined sources1
Modified residuei1204PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylation by CK2 disrupt the intramolecular binding between the bromo domain 2 and the NPS region and promotes binding between the NPS and the BID regions, leading to activate the protein and promote binding to acetylated histones. In absence of phosphorylation, BRD4 does not localize to p53/TP53 target gene promoters, phosphorylation promoting recruitment to p53/TP53 target promoters.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

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EPDi
O60885

jPOST - Japan Proteome Standard Repository/Database

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jPOSTi
O60885

MassIVE - Mass Spectrometry Interactive Virtual Environment

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MassIVEi
O60885

MaxQB - The MaxQuant DataBase

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MaxQBi
O60885

PaxDb, a database of protein abundance averages across all three domains of life

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PaxDbi
O60885

PeptideAtlas

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PeptideAtlasi
O60885

PRoteomics IDEntifications database

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PRIDEi
O60885

ProteomicsDB: a multi-organism proteome resource

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ProteomicsDBi
49651 [O60885-1]
49652 [O60885-2]
62139

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

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iPTMneti
O60885

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

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PhosphoSitePlusi
O60885

SwissPalm database of S-palmitoylation events

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SwissPalmi
O60885

Miscellaneous databases

CutDB - Proteolytic event database

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PMAP-CutDBi
O60885

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the ‘Expression’ section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified ‘at protein level’. <br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

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Bgeei
ENSG00000141867 Expressed in 239 organ(s), highest expression level in amniotic fluid

ExpressionAtlas, Differential and Baseline Expression

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ExpressionAtlasi
O60885 baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

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Genevisiblei
O60885 HS

Organism-specific databases

Human Protein Atlas

More...
HPAi
CAB068177
CAB068178
HPA015055
HPA061646

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Binds acetylated histone H4 (PubMed:29176719).

Interacts with p53/TP53; the interaction is direct (PubMed:23317504).

Interacts (via CTD region) with CDK9 and CCNT1, acting as an associated component of P-TEFb complex (PubMed:16109376, PubMed:16109377, PubMed:23317504, PubMed:24360279).

Interacts with RELA (when acetylated at 'Lys-310')(PubMed:19103749).

Interacts (via NET domain) with NSD3, CHD4, BICRA and ATAD5 (PubMed:21555454,PubMed:29176719). The interaction with BICRA bridges BRD4 to the GBAF complex (PubMed:29374058, PubMed:16109376, PubMed:16109377, PubMed:19103749, PubMed:21555454, PubMed:23317504).

Interacts (via NET domain) with JMJD6 (via JmjC and N-terminal domains); the interaction is stronger in presence of ssRNA and recruits JMJD6 on distal enhancers (PubMed:24360279, PubMed:21555454, PubMed:29176719).

Interacts with NSD3 (PubMed:29176719). Isoform B: interacts with NCAPD3 and SMC2 (PubMed:23728299).

9 Publications

(Microbial infection) Interacts with bovine papillomavirus type 1 regulatory protein E2. This interactions may serve for the tethering of viral genomes to host mitotic chromosomes allowing successful partitioning of the viral genome during cell division.

1 Publication

(Microbial infection) Interacts with Epstein-Barr virus (EBV) protein EBNA1; this interaction facilitates transcriptional activation by EBNA1.

1 Publication

(Microbial infection) Interacts with human herpes virus-8 (HHV-8) protein LANA.

1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

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BioGridi
117036, 119 interactors

CORUM comprehensive resource of mammalian protein complexes

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CORUMi
O60885

Database of interacting proteins

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DIPi
DIP-39776N

Protein interaction database and analysis system

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IntActi
O60885, 52 interactors

Molecular INTeraction database

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MINTi
O60885

STRING: functional protein association networks

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STRINGi
9606.ENSP00000263377

Chemistry databases

BindingDB database of measured binding affinities

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BindingDBi
O60885

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

11362
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

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SMRi
O60885

Database of comparative protein structure models

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ModBasei
Search...

Protein Data Bank in Europe - Knowledge Base

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PDBe-KBi
Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

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EvolutionaryTracei
O60885

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini75 – 147Bromo 1PROSITE-ProRule annotationAdd BLAST73
Domaini368 – 440Bromo 2PROSITE-ProRule annotationAdd BLAST73
Domaini600 – 682NETPROSITE-ProRule annotationAdd BLAST83

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni484 – 503NPS regionAdd BLAST20
Regioni524 – 579BID regionAdd BLAST56
Regioni1047 – 1362C-terminal (CTD) regionAdd BLAST316

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Family and Domains’ section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi535 – 594Lys-richAdd BLAST60
Compositional biasi692 – 717Ser-richAdd BLAST26
Compositional biasi703 – 714Poly-SerAdd BLAST12
Compositional biasi738 – 743Poly-His6
Compositional biasi757 – 761Poly-Pro5
Compositional biasi764 – 770Poly-Pro7
Compositional biasi771 – 775Poly-Gln5
Compositional biasi776 – 783Poly-Pro8
Compositional biasi954 – 964Poly-ProAdd BLAST11
Compositional biasi974 – 986Poly-ProAdd BLAST13
Compositional biasi1011 – 1014Poly-Pro4
Compositional biasi1028 – 1033Poly-Pro6
Compositional biasi1283 – 1300Poly-GlnAdd BLAST18
Compositional biasi1301 – 1308Poly-Ala8
Compositional biasi1335 – 1338Poly-Arg4

<p>This subsection of the ‘Family and domains’ section provides general information on the biological role of a domain. The term ‘domain’ is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The NET domain mediates interaction with a number of chromatin proteins involved in transcription regulation (NSD3, JMJD6, CHD4, GLTSCR1 and ATAD5).1 Publication
The C-terminal (CTD) region mediates interaction and recruitment of CDK9 and CCNT1 subunits of the P-TEFb complex (PubMed:16109376, PubMed:16109377). It is also required for maintenance of higher-order chromatin structure (PubMed:22334664).3 Publications
The 2 bromo domains mediate specific binding to acetylated histones via Asn-140 and Asn-433, respectively (PubMed:20871596). The exact combination of modified histone tails required to recruit BRD4 to target genes is still unclear. The first bromo domain has high affinity for acetylated histone H4 tail, whereas the second bromo domain recognizes multiply acetylated marks in histone H3 (PubMed:22464331). A number of specific inhibitors bind competitively to acetyl-lysine-binding residues Asn-140 and Asn-433, promoting removal from acetylated histones. Many of these inhibitors are benzodiazepine derivatives (PubMed:22137933, PubMed:22136404, PubMed:23517011, PubMed:23530754).6 Publications

Keywords - Domaini

Bromodomain, Repeat

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

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eggNOGi
KOG1474 Eukaryota
COG5076 LUCA

Ensembl GeneTree

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GeneTreei
ENSGT00940000154549

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

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HOGENOMi
HOG000231200

InParanoid: Eukaryotic Ortholog Groups

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InParanoidi
O60885

KEGG Orthology (KO)

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KOi
K11722

Identification of Orthologs from Complete Genome Data

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OMAi
ERMRWAR

Database of Orthologous Groups

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OrthoDBi
EOG091G01QC

Database for complete collections of gene phylogenies

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PhylomeDBi
O60885

TreeFam database of animal gene trees

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TreeFami
TF317345

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

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Gene3Di
1.20.1270.220, 1 hit
1.20.920.10, 2 hits

Integrated resource of protein families, domains and functional sites

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InterProi
View protein in InterPro
IPR031354 BRD4_CDT
IPR001487 Bromodomain
IPR036427 Bromodomain-like_sf
IPR018359 Bromodomain_CS
IPR027353 NET_dom
IPR038336 NET_sf

Pfam protein domain database

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Pfami
View protein in Pfam
PF17035 BET, 1 hit
PF17105 BRD4_CDT, 1 hit
PF00439 Bromodomain, 2 hits

Protein Motif fingerprint database; a protein domain database

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PRINTSi
PR00503 BROMODOMAIN

Simple Modular Architecture Research Tool; a protein domain database

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SMARTi
View protein in SMART
SM00297 BROMO, 2 hits

Superfamily database of structural and functional annotation

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SUPFAMi
SSF47370 SSF47370, 2 hits

PROSITE; a protein domain and family database

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PROSITEi
View protein in PROSITE
PS00633 BROMODOMAIN_1, 1 hit
PS50014 BROMODOMAIN_2, 2 hits
PS51525 NET, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (3+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 3 <p>This subsection of the ‘Sequence’ section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform A (identifier: O60885-1) [UniParc]FASTAAdd to basket
Also known as: Brd4L, Long

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSAESGPGTR LRNLPVMGDG LETSQMSTTQ AQAQPQPANA ASTNPPPPET
60 70 80 90 100
SNPNKPKRQT NQLQYLLRVV LKTLWKHQFA WPFQQPVDAV KLNLPDYYKI
110 120 130 140 150
IKTPMDMGTI KKRLENNYYW NAQECIQDFN TMFTNCYIYN KPGDDIVLMA
160 170 180 190 200
EALEKLFLQK INELPTEETE IMIVQAKGRG RGRKETGTAK PGVSTVPNTT
210 220 230 240 250
QASTPPQTQT PQPNPPPVQA TPHPFPAVTP DLIVQTPVMT VVPPQPLQTP
260 270 280 290 300
PPVPPQPQPP PAPAPQPVQS HPPIIAATPQ PVKTKKGVKR KADTTTPTTI
310 320 330 340 350
DPIHEPPSLP PEPKTTKLGQ RRESSRPVKP PKKDVPDSQQ HPAPEKSSKV
360 370 380 390 400
SEQLKCCSGI LKEMFAKKHA AYAWPFYKPV DVEALGLHDY CDIIKHPMDM
410 420 430 440 450
STIKSKLEAR EYRDAQEFGA DVRLMFSNCY KYNPPDHEVV AMARKLQDVF
460 470 480 490 500
EMRFAKMPDE PEEPVVAVSS PAVPPPTKVV APPSSSDSSS DSSSDSDSST
510 520 530 540 550
DDSEEERAQR LAELQEQLKA VHEQLAALSQ PQQNKPKKKE KDKKEKKKEK
560 570 580 590 600
HKRKEEVEEN KKSKAKEPPP KKTKKNNSSN SNVSKKEPAP MKSKPPPTYE
610 620 630 640 650
SEEEDKCKPM SYEEKRQLSL DINKLPGEKL GRVVHIIQSR EPSLKNSNPD
660 670 680 690 700
EIEIDFETLK PSTLRELERY VTSCLRKKRK PQAEKVDVIA GSSKMKGFSS
710 720 730 740 750
SESESSSESS SSDSEDSETE MAPKSKKKGH PGREQKKHHH HHHQQMQQAP
760 770 780 790 800
APVPQQPPPP PQQPPPPPPP QQQQQPPPPP PPPSMPQQAA PAMKSSPPPF
810 820 830 840 850
IATQVPVLEP QLPGSVFDPI GHFTQPILHL PQPELPPHLP QPPEHSTPPH
860 870 880 890 900
LNQHAVVSPP ALHNALPQQP SRPSNRAAAL PPKPARPPAV SPALTQTPLL
910 920 930 940 950
PQPPMAQPPQ VLLEDEEPPA PPLTSMQMQL YLQQLQKVQP PTPLLPSVKV
960 970 980 990 1000
QSQPPPPLPP PPHPSVQQQL QQQPPPPPPP QPQPPPQQQH QPPPRPVHLQ
1010 1020 1030 1040 1050
PMQFSTHIQQ PPPPQGQQPP HPPPGQQPPP PQPAKPQQVI QHHHSPRHHK
1060 1070 1080 1090 1100
SDPYSTGHLR EAPSPLMIHS PQMSQFQSLT HQSPPQQNVQ PKKQELRAAS
1110 1120 1130 1140 1150
VVQPQPLVVV KEEKIHSPII RSEPFSPSLR PEPPKHPESI KAPVHLPQRP
1160 1170 1180 1190 1200
EMKPVDVGRP VIRPPEQNAP PPGAPDKDKQ KQEPKTPVAP KKDLKIKNMG
1210 1220 1230 1240 1250
SWASLVQKHP TTPSSTAKSS SDSFEQFRRA AREKEEREKA LKAQAEHAEK
1260 1270 1280 1290 1300
EKERLRQERM RSREDEDALE QARRAHEEAR RRQEQQQQQR QEQQQQQQQQ
1310 1320 1330 1340 1350
AAAVAAAATP QAQSSQPQSM LDQQRELARK REQERRRREA MAATIDMNFQ
1360
SDLLSIFEEN LF
Length:1,362
Mass (Da):152,219
Last modified:January 31, 2002 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:iD52EFE1CF9960907
GO
Isoform C (identifier: O60885-2) [UniParc]FASTAAdd to basket
Also known as: Brd4S, Short

The sequence of this isoform differs from the canonical sequence as follows:
     720-722: EMA → GPA
     723-1362: Missing.

Show »
Length:722
Mass (Da):80,463
Checksum:iFF040EE016B37F87
GO
Isoform B (identifier: O60885-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     720-1362: EMAPKSKKKG...LLSIFEENLF → AFCTSGDFVS...ECARCCVGCS

Note: Does not contain the C-terminal (CTD) region required to recruit the P-TEFb complex.
Show »
Length:794
Mass (Da):88,289
Checksum:iACB01CC53AA4C431
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M0QYW0M0QYW0_HUMAN
Bromodomain containing 4, isoform C...
BRD4 hCG_2000917
146Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0QZD9M0QZD9_HUMAN
Bromodomain-containing protein 4
BRD4
572Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0R0H4M0R0H4_HUMAN
Bromodomain-containing protein 4
BRD4
105Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence AAC27978 differs from that shown. Reason: Erroneous initiation. Extended N-terminus.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_04191937P → S1 PublicationCorresponds to variant dbSNP:rs35177876Ensembl.1
Natural variantiVAR_041920371A → G1 PublicationCorresponds to variant dbSNP:rs55805532Ensembl.1
Natural variantiVAR_041921563S → N1 PublicationCorresponds to variant dbSNP:rs55970906Ensembl.1
Natural variantiVAR_041922598T → S1 PublicationCorresponds to variant dbSNP:rs34362023Ensembl.1
Natural variantiVAR_041923669R → H1 PublicationCorresponds to variant dbSNP:rs35824241Ensembl.1
Natural variantiVAR_0484271097R → H. Corresponds to variant dbSNP:rs35676845Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_047671720 – 1362EMAPK…EENLF → AFCTSGDFVSPGPSPYHSHV QCGRFREMLRWFLVDVEQTA AGQPHRQSAAGPAITWAPAI AYPSPECARCCVGCS in isoform B. 1 PublicationAdd BLAST643
Alternative sequenceiVSP_010902720 – 722EMA → GPA in isoform C. 1 Publication3
Alternative sequenceiVSP_010903723 – 1362Missing in isoform C. 1 PublicationAdd BLAST640

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

More...
GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
AF386649 mRNA Translation: AAL26987.1
Y12059 mRNA Translation: CAA72780.1
AC004798 Genomic DNA Translation: AAC27978.1 Different initiation.
AC003111 Genomic DNA No translation available.
AC005776 Genomic DNA No translation available.
CH471106 Genomic DNA Translation: EAW84470.1
BC035266 mRNA Translation: AAH35266.1
AY166680 mRNA Translation: AAO22237.1 Different termination.

The Consensus CDS (CCDS) project

More...
CCDSi
CCDS12328.1 [O60885-1]
CCDS46004.1 [O60885-2]
CCDS82307.1 [O60885-3]

NCBI Reference Sequences

More...
RefSeqi
NP_001317313.1, NM_001330384.1 [O60885-3]
NP_055114.1, NM_014299.2 [O60885-2]
NP_490597.1, NM_058243.2 [O60885-1]
XP_011526156.1, XM_011527854.1 [O60885-1]
XP_011526158.1, XM_011527856.2 [O60885-2]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...
Ensembli
ENST00000263377; ENSP00000263377; ENSG00000141867 [O60885-1]
ENST00000360016; ENSP00000353112; ENSG00000141867 [O60885-3]
ENST00000371835; ENSP00000360901; ENSG00000141867 [O60885-2]

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
23476

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
hsa:23476

UCSC genome browser

More...
UCSCi
uc002nar.4 human [O60885-1]

Keywords - Coding sequence diversityi

Alternative splicing, Chromosomal rearrangement, Polymorphism

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

<p>This subsection of the <a href="http://www.uniprot.org/manual/cross_references_section">Cross-references</a> section provides links to various web resources that are relevant for a specific protein.<p><a href='/help/web_resource' target='_top'>More...</a></p>Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF386649 mRNA Translation: AAL26987.1
Y12059 mRNA Translation: CAA72780.1
AC004798 Genomic DNA Translation: AAC27978.1 Different initiation.
AC003111 Genomic DNA No translation available.
AC005776 Genomic DNA No translation available.
CH471106 Genomic DNA Translation: EAW84470.1
BC035266 mRNA Translation: AAH35266.1
AY166680 mRNA Translation: AAO22237.1 Different termination.
CCDSiCCDS12328.1 [O60885-1]
CCDS46004.1 [O60885-2]
CCDS82307.1 [O60885-3]
RefSeqiNP_001317313.1, NM_001330384.1 [O60885-3]
NP_055114.1, NM_014299.2 [O60885-2]
NP_490597.1, NM_058243.2 [O60885-1]
XP_011526156.1, XM_011527854.1 [O60885-1]
XP_011526158.1, XM_011527856.2 [O60885-2]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2I8NNMR-A352-457[»]
2LSPNMR-B333-460[»]
2MJVNMR-B333-460[»]
2N3KNMR-A600-678[»]
2NCZNMR-A601-683[»]
2ND0NMR-A601-683[»]
2ND1NMR-A601-683[»]
2NNUX-ray1.59B1343-1362[»]
2OSSX-ray1.35A44-168[»]
2OUOX-ray1.89A333-460[»]
2YELX-ray1.65A44-168[»]
2YEMX-ray2.30A/B333-460[»]
3MXFX-ray1.60A42-168[»]
3P5OX-ray1.60A44-168[»]
3SVFX-ray1.98A44-168[»]
3SVGX-ray1.68A44-168[»]
3U5JX-ray1.60A44-168[»]
3U5KX-ray1.80A/B/C/D44-168[»]
3U5LX-ray1.39A44-168[»]
3UVWX-ray1.37A44-168[»]
3UVXX-ray1.91A44-168[»]
3UVYX-ray2.02A44-168[»]
3UW9X-ray2.30A/B/C/D44-168[»]
3ZYUX-ray1.50A/B44-168[»]
4A9LX-ray1.60A44-168[»]
4BJXX-ray1.59A44-168[»]
4BW1X-ray1.40A44-168[»]
4BW2X-ray1.92A44-168[»]
4BW3X-ray1.50A44-168[»]
4BW4X-ray1.67A44-168[»]
4C66X-ray1.87A44-167[»]
4C67X-ray1.55A44-168[»]
4CFKX-ray1.55A44-168[»]
4CFLX-ray1.32A44-168[»]
4CL9X-ray1.40A44-168[»]
4CLBX-ray1.60A44-168[»]
4DONX-ray1.52A44-166[»]
4E96X-ray1.92A44-168[»]
4F3IX-ray1.40A44-168[»]
4GPJX-ray1.60A44-168[»]
4HBVX-ray1.63A42-168[»]
4HBWX-ray1.69A42-168[»]
4HBXX-ray1.62A42-168[»]
4HBYX-ray1.59A44-168[»]
4HXKX-ray1.61A44-167[»]
4HXLX-ray1.52A44-167[»]
4HXMX-ray1.50A44-166[»]
4HXNX-ray1.49A44-167[»]
4HXOX-ray1.76A44-167[»]
4HXPX-ray1.73A44-166[»]
4HXRX-ray1.53A44-167[»]
4HXSX-ray1.43A44-166[»]
4IOOX-ray1.25A44-168[»]
4IOQX-ray1.50A44-168[»]
4IORX-ray1.40A44-168[»]
4J0RX-ray1.72A44-168[»]
4J0SX-ray1.84A44-168[»]
4J3IX-ray1.24A44-168[»]
4KV1X-ray1.50A/B41-168[»]
4KV4X-ray2.00A351-459[»]
4LR6X-ray1.29A42-168[»]
4LRGX-ray2.21A42-168[»]
4LYIX-ray1.30A44-168[»]
4LYSX-ray1.83A44-168[»]
4LYWX-ray1.95A44-168[»]
4LZRX-ray1.85A44-168[»]
4LZSX-ray2.20A44-168[»]
4MENX-ray1.81A44-168[»]
4MEOX-ray1.72A44-168[»]
4MEPX-ray1.85A44-168[»]
4MEQX-ray1.77A44-168[»]
4MR3X-ray1.68A44-168[»]
4MR4X-ray1.66A44-168[»]
4NQMX-ray1.58A44-168[»]
4NR8X-ray1.64A44-168[»]
4NUCX-ray1.40A44-168[»]
4NUDX-ray1.20A44-168[»]
4NUEX-ray1.30A44-168[»]
4O70X-ray1.55A/B44-168[»]
4O71X-ray1.36A/B44-168[»]
4O72X-ray1.40A44-168[»]
4O74X-ray1.45A/B44-168[»]
4O75X-ray1.55A44-168[»]
4O76X-ray1.70A/B/C/D44-168[»]
4O77X-ray2.00A/B44-168[»]
4O78X-ray1.34A44-168[»]
4O7AX-ray1.34A44-168[»]
4O7BX-ray1.50A44-168[»]
4O7CX-ray1.55A44-168[»]
4O7EX-ray1.85A/B44-168[»]
4O7FX-ray1.80A/B44-168[»]
4OGIX-ray1.73A/B44-168[»]
4OGJX-ray1.65A/B44-168[»]
4PCEX-ray1.29A44-168[»]
4PCIX-ray1.25A44-168[»]
4PS5X-ray1.40A/B44-168[»]
4QB3X-ray0.94A44-168[»]
4QR3X-ray1.37A44-166[»]
4QR4X-ray1.28A44-166[»]
4QR5X-ray1.41A44-166[»]
4QZSX-ray1.45A44-168[»]
4UIXX-ray1.58A/B/C44-168[»]
4UIYX-ray1.30A44-168[»]
4UIZX-ray1.19A44-168[»]
4UYDX-ray1.37A44-183[»]
4WHWX-ray1.34A44-168[»]
4WIVX-ray1.56A44-168[»]
4X2IX-ray1.20A42-166[»]
4XY9X-ray1.83A42-168[»]
4XYAX-ray2.05A42-168[»]
4YH3X-ray1.60A44-170[»]
4YH4X-ray1.33A44-170[»]
4Z1QX-ray1.40A/B42-167[»]
4Z1SX-ray1.06A/B42-166[»]
4Z93X-ray1.27A349-460[»]
4ZC9X-ray0.99A44-168[»]
4ZW1X-ray1.75A44-168[»]
5A5SX-ray1.36A44-168[»]
5A85X-ray1.72A44-168[»]
5ACYX-ray2.01A/B44-168[»]
5AD2X-ray2.01A/B44-168[»]
5AD3X-ray1.49A/B44-168[»]
5BT4X-ray1.50A/B/C44-168[»]
5CFWX-ray1.15A44-168[»]
5COIX-ray1.62A44-168[»]
5CP5X-ray1.79A44-168[»]
5CPEX-ray1.62A44-168[»]
5CQTX-ray1.60A44-168[»]
5CRMX-ray1.99A44-168[»]
5CRZX-ray2.12A44-168[»]
5CS8X-ray1.62A44-168[»]
5CTLX-ray2.51A44-168[»]
5CY9X-ray1.55A44-168[»]
5D0CX-ray1.49A44-168[»]
5D24X-ray1.65A43-168[»]
5D25X-ray1.70A43-168[»]
5D26X-ray1.82A42-168[»]
5D3HX-ray1.70A44-168[»]
5D3JX-ray1.70A43-168[»]
5D3LX-ray1.50A42-168[»]
5D3NX-ray2.15A43-168[»]
5D3PX-ray1.95A42-168[»]
5D3RX-ray2.20A42-168[»]
5D3SX-ray1.75A44-168[»]
5D3TX-ray1.93A42-168[»]
5DLXX-ray1.90A44-168[»]
5DLZX-ray1.70A44-168[»]
5DW2X-ray1.12A44-170[»]
5DX4X-ray2.30A44-168[»]
5E0RX-ray1.35A44-168[»]
5EGUX-ray2.21A/B/C/D44-168[»]
5EI4X-ray1.05A44-168[»]
5EISX-ray1.60A44-168[»]
5F5ZX-ray1.76A44-168[»]
5F60X-ray1.35A44-168[»]
5F61X-ray1.45A/B44-168[»]
5F62X-ray1.35A44-168[»]
5F63X-ray1.45A44-168[»]
5FBXX-ray1.85A44-168[»]
5H21X-ray1.59A44-167[»]
5HCLX-ray1.50A44-168[»]
5HLSX-ray2.18A42-168[»]
5HM0X-ray1.40A42-168[»]
5HQ5X-ray1.60A44-168[»]
5HQ6X-ray1.95A44-166[»]
5HQ7X-ray1.90A44-167[»]
5I80X-ray1.45A42-167[»]
5I88X-ray1.40A42-167[»]
5IGKX-ray1.70A44-168[»]
5JWMX-ray1.71A/B333-460[»]
5KDHX-ray1.50A44-168[»]
5KHMX-ray1.48A/B44-168[»]
5KJ0X-ray1.51A44-168[»]
5KU3X-ray1.14A42-167[»]
5LJ1X-ray1.90A42-168[»]
5LJ2X-ray1.19A42-168[»]
5LRQX-ray1.70A42-163[»]
5LUUX-ray1.61A44-168[»]
5M39X-ray1.38A/B42-168[»]
5M3AX-ray1.65A44-168[»]
5MKZX-ray1.62A44-168[»]
5MLIX-ray1.63A42-168[»]
5N2MX-ray1.54A44-168[»]
5NNCX-ray2.22A/B44-168[»]
5NNDX-ray1.82A/B44-168[»]
5NNEX-ray1.15A44-168[»]
5NNFX-ray1.15A44-168[»]
5NNGX-ray1.20A44-168[»]
5O97X-ray1.30A44-168[»]
5OVBX-ray1.95A/B44-168[»]
5OWMX-ray1.50A44-168[»]
5OWWX-ray1.50A/B/C/D44-168[»]
5T35X-ray2.70A/E333-460[»]
5TI2X-ray1.65A44-168[»]
5TI3X-ray1.70A44-168[»]
5TI4X-ray1.62A44-168[»]
5TI5X-ray1.83A44-168[»]
5TI6X-ray1.70A44-168[»]
5TI7X-ray1.65A44-168[»]
5U28X-ray1.80A44-180[»]
5U2CX-ray3.30A/B342-460[»]
5U2EX-ray1.99A/B42-180[»]
5U2FX-ray2.52A/B42-180[»]
5UEOX-ray1.85A/B352-457[»]
5UEPX-ray1.77A352-457[»]
5UEQX-ray1.70A352-457[»]
5UERX-ray1.87A352-457[»]
5UESX-ray1.62A352-457[»]
5UETX-ray2.29A352-457[»]
5UEUX-ray2.26A/B352-457[»]
5UEVX-ray1.94A352-457[»]
5UEXX-ray2.29A352-457[»]
5UEYX-ray2.41A352-457[»]
5UEZX-ray1.51A352-457[»]
5UF0X-ray1.35A352-457[»]
5ULAX-ray1.50A/B44-168[»]
5UOOX-ray1.69A333-460[»]
5UVSX-ray2.15A352-457[»]
5UVTX-ray1.67A352-457[»]
5UVUX-ray1.66A352-457[»]
5UVVX-ray1.99A/B352-457[»]
5UVWX-ray2.14A/B/C57-165[»]
5UVXX-ray1.53A/B352-457[»]
5UVYX-ray2.25A352-457[»]
5UVZX-ray1.63A352-457[»]
5V67X-ray1.78A44-168[»]
5VBOX-ray1.30A44-168[»]
5VBPX-ray1.83A/B44-168[»]
5VOMX-ray1.67A/B44-168[»]
5VZSX-ray1.71A/B42-168[»]
5W55X-ray1.35A42-168[»]
5WA5X-ray1.17A42-168[»]
5WMAX-ray1.40A44-168[»]
5WMDX-ray1.27A44-168[»]
5WMGX-ray1.19A44-168[»]
5WUUX-ray1.72A44-167[»]
5XHYX-ray1.98A44-166[»]
5XI2X-ray1.91A44-166[»]
5XI3X-ray1.67A44-166[»]
5XI4X-ray1.49A44-166[»]
5Y1YX-ray1.91A44-167[»]
5Y8CX-ray1.42A44-166[»]
5Y8WX-ray1.76A44-168[»]
5Y8YX-ray1.87A44-168[»]
5Y8ZX-ray1.84A44-168[»]
5Y93X-ray1.62A44-168[»]
5Y94X-ray2.00A44-168[»]
5YOUX-ray1.50A42-168[»]
5YOVX-ray1.45A42-168[»]
5YQXX-ray1.82A44-167[»]
5Z1RX-ray1.62A44-168[»]
5Z1SX-ray1.42A44-168[»]
5Z1TX-ray1.42A44-168[»]
5Z5TX-ray1.99A44-167[»]
5Z5UX-ray1.63A44-167[»]
5Z5VX-ray1.66A44-167[»]
5Z8GX-ray1.70A44-168[»]
5Z8RX-ray2.00A44-168[»]
5Z8ZX-ray1.80A44-168[»]
5Z90X-ray1.80A44-168[»]
5Z9CNMR-A53-168[»]
5Z9KX-ray1.89A44-168[»]
6AFRX-ray2.00A44-168[»]
6AJVX-ray1.45A42-168[»]
6AJWX-ray1.40A42-168[»]
6AJXX-ray1.89A42-168[»]
6AJYX-ray1.60A42-168[»]
6AJZOther1.30A42-168[»]
6BN7X-ray3.50C42-168[»]
6BN8X-ray3.99C42-168[»]
6BN9X-ray4.38C42-168[»]
6BNBX-ray6.34C42-168[»]
6BNHNMR-A601-683[»]
6BOYX-ray3.33C42-168[»]
6C7QX-ray1.51A333-460[»]
6C7RX-ray1.50A44-165[»]
6CD4X-ray1.23A42-168[»]
6CD5X-ray1.58A44-168[»]
6CISX-ray1.51A44-166[»]
6CIYX-ray1.68A44-168[»]
6CJ1X-ray1.53A44-166[»]
6CJ2X-ray1.47A42-166[»]
6CKRX-ray1.62A/B44-168[»]
6CKSX-ray1.72A44-168[»]
6CZUX-ray1.47A42-170[»]
6CZVX-ray1.88A42-170[»]
6DJCX-ray1.46A/B44-173[»]
6DL2X-ray1.47A44-168[»]
6DMJX-ray1.15A44-168[»]
6DMLX-ray1.50A44-168[»]
6DNEX-ray2.96A/B44-477[»]
6DUVX-ray1.80A/B347-458[»]
6E4AX-ray1.26A/B44-170[»]
6FFDX-ray1.83A347-463[»]
6FNXX-ray1.19A44-168[»]
6FO5X-ray0.95A44-168[»]
6FSYX-ray1.34A42-168[»]
6FT3X-ray1.28A42-168[»]
6FT4X-ray1.34A42-168[»]
6G0OX-ray1.40A42-168[»]
6G0PX-ray1.30A42-168[»]
6G0QX-ray1.40A42-168[»]
6G0RX-ray1.25A42-168[»]
6G0SX-ray1.48A/B42-168[»]
6HDQX-ray1.70A44-168[»]
6MAUX-ray2.11A44-170[»]
6MH1X-ray1.60A/B44-168[»]
6MH7X-ray1.74A/B44-168[»]
6MNLNMR-B333-460[»]
6Q3YX-ray1.20A/B42-168[»]
6Q3ZX-ray2.00A/B44-168[»]
6S25X-ray1.10A44-168[»]
6SE4X-ray1.38A44-168[»]
SMRiO60885
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGridi117036, 119 interactors
CORUMiO60885
DIPiDIP-39776N
IntActiO60885, 52 interactors
MINTiO60885
STRINGi9606.ENSP00000263377

Chemistry databases

BindingDBiO60885
ChEMBLiCHEMBL1163125
DrugCentraliO60885
GuidetoPHARMACOLOGYi1945

PTM databases

iPTMnetiO60885
PhosphoSitePlusiO60885
SwissPalmiO60885

Polymorphism and mutation databases

BioMutaiBRD4

Proteomic databases

EPDiO60885
jPOSTiO60885
MassIVEiO60885
MaxQBiO60885
PaxDbiO60885
PeptideAtlasiO60885
PRIDEiO60885
ProteomicsDBi49651 [O60885-1]
49652 [O60885-2]
62139

Protocols and materials databases

ABCD curated depository of sequenced antibodies

More...
ABCDi
O60885

Genome annotation databases

EnsembliENST00000263377; ENSP00000263377; ENSG00000141867 [O60885-1]
ENST00000360016; ENSP00000353112; ENSG00000141867 [O60885-3]
ENST00000371835; ENSP00000360901; ENSG00000141867 [O60885-2]
GeneIDi23476
KEGGihsa:23476
UCSCiuc002nar.4 human [O60885-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...
CTDi
23476
DisGeNETi23476

GeneCards: human genes, protein and diseases

More...
GeneCardsi
BRD4
HGNCiHGNC:13575 BRD4
HPAiCAB068177
CAB068178
HPA015055
HPA061646
MalaCardsiBRD4
MIMi608749 gene
neXtProtiNX_O60885
OpenTargetsiENSG00000141867
Orphaneti443167 NUT midline carcinoma
PharmGKBiPA25416

GenAtlas: human gene database

More...
GenAtlasi
Search...

Phylogenomic databases

eggNOGiKOG1474 Eukaryota
COG5076 LUCA
GeneTreeiENSGT00940000154549
HOGENOMiHOG000231200
InParanoidiO60885
KOiK11722
OMAiERMRWAR
OrthoDBiEOG091G01QC
PhylomeDBiO60885
TreeFamiTF317345

Enzyme and pathway databases

SIGNORiO60885

Miscellaneous databases

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...
ChiTaRSi
BRD4 human
EvolutionaryTraceiO60885

The Gene Wiki collection of pages on human genes and proteins

More...
GeneWikii
BRD4

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...
GenomeRNAii
23476
PharosiO60885
PMAP-CutDBiO60885

Protein Ontology

More...
PROi
PR:O60885

The Stanford Online Universal Resource for Clones and ESTs

More...
SOURCEi
Search...

Gene expression databases

BgeeiENSG00000141867 Expressed in 239 organ(s), highest expression level in amniotic fluid
ExpressionAtlasiO60885 baseline and differential
GenevisibleiO60885 HS

Family and domain databases

Gene3Di1.20.1270.220, 1 hit
1.20.920.10, 2 hits
InterProiView protein in InterPro
IPR031354 BRD4_CDT
IPR001487 Bromodomain
IPR036427 Bromodomain-like_sf
IPR018359 Bromodomain_CS
IPR027353 NET_dom
IPR038336 NET_sf
PfamiView protein in Pfam
PF17035 BET, 1 hit
PF17105 BRD4_CDT, 1 hit
PF00439 Bromodomain, 2 hits
PRINTSiPR00503 BROMODOMAIN
SMARTiView protein in SMART
SM00297 BROMO, 2 hits
SUPFAMiSSF47370 SSF47370, 2 hits
PROSITEiView protein in PROSITE
PS00633 BROMODOMAIN_1, 1 hit
PS50014 BROMODOMAIN_2, 2 hits
PS51525 NET, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiBRD4_HUMAN
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O60885
Secondary accession number(s): O60433
, Q4G0X8, Q86YS8, Q96PD3
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 31, 2002
Last modified: October 16, 2019
This is version 188 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  5. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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