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Protein

DNA/RNA-binding protein KIN17

Gene

KIN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo (By similarity). Binds via its C-terminal domain to RNA in vitro.By similarity6 Publications

Miscellaneous

Recognized by antibodies directed against the RecA protein.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri28 – 50C2H2-typeSequence analysisAdd BLAST23

GO - Molecular functioni

  • DNA binding Source: ProtInc
  • double-stranded DNA binding Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • RNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionDNA-binding, RNA-binding
Biological processDNA damage, DNA recombination, DNA repair, DNA replication, Host-virus interaction, mRNA processing, Stress response
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-8876725 Protein methylation

Names & Taxonomyi

Protein namesi
Recommended name:
DNA/RNA-binding protein KIN17
Alternative name(s):
Binding to curved DNA
KIN, antigenic determinant of recA protein homolog
Gene namesi
Name:KINImported
Synonyms:BTCD, KIN17
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000151657.11
HGNCiHGNC:6327 KIN
MIMi601720 gene
neXtProtiNX_O60870

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi135K → R: Almost complete loss of in vitro methylation by METTL22. 1 Publication1
Mutagenesisi302K → E: Significant reduction of RNA-binding activity. 1 Publication1
Mutagenesisi391K → E: Significant reduction of RNA-binding activity. 1 Publication1

Organism-specific databases

DisGeNETi22944
OpenTargetsiENSG00000151657
PharmGKBiPA30113

Polymorphism and mutation databases

BioMutaiKIN

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002891341 – 393DNA/RNA-binding protein KIN17Add BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei135N6,N6,N6-trimethyllysine; by METTL22; in vitroCombined sources1 Publication1
Modified residuei135N6-methyllysineCombined sources1

Keywords - PTMi

Methylation

Proteomic databases

EPDiO60870
MaxQBiO60870
PaxDbiO60870
PeptideAtlasiO60870
PRIDEiO60870
ProteomicsDBi49638

PTM databases

iPTMnetiO60870
PhosphoSitePlusiO60870

Expressioni

Tissue specificityi

Ubiquitously expressed in all tissues examined, with highest levels in skeletal muscle, heart and testis. Differentially expressed in non-tumorigenic and tumorigenic cell lines. Highly expressed in proliferating epithelial keratinocyte cells in vitro (at protein level).2 Publications

Inductioni

By UVC irradiation in quiescent primary fibroblasts. By mitomycin C in human melanoma MeWO cells.2 Publications

Gene expression databases

BgeeiENSG00000151657 Expressed in 217 organ(s), highest expression level in lung
CleanExiHS_KIN
ExpressionAtlasiO60870 baseline and differential
GenevisibleiO60870 HS

Organism-specific databases

HPAiCAB016339
HPA038700

Interactioni

Subunit structurei

Associated with DNA polymerase alpha, RFC1 and cyclin A, in multiprotein DNA replication complexes. Also associates with replication origins at the G1/S phase boundary and throughout the S phase in vivo.1 Publication
(Microbial infection) Interacts with SV40 large T antigen.1 Publication

Protein-protein interaction databases

BioGridi116600, 13 interactors
CORUMiO60870
IntActiO60870, 3 interactors
MINTiO60870
STRINGi9606.ENSP00000368881

Structurei

Secondary structure

1393
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO60870
SMRiO60870
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60870

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni51 – 160Winged helix-turn-helix (wHTH)Add BLAST110
Regioni284 – 334C-terminal subdomain A1 PublicationAdd BLAST51
Regioni340 – 391C-terminal subdomain B1 PublicationAdd BLAST52

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili147 – 180Sequence analysisAdd BLAST34
Coiled coili250 – 277Sequence analysisAdd BLAST28

Domaini

The C-terminal domain (268-393) is organized into 2 subdomains that bear structural similarities to SH3-like domains. Both subdomains adopt a similar 5-stranded beta-barrel-like fold and are connected to each other by a short linker of 5 residues. The 5 beta-sheets are packed at approximately right angles against each other. A highly conserved groove formed at the interface between the 2 subdomains, comprised of Lys residues 302 and 391 and other positively charged residues, may possibly be the site of RNA-binding.1 Publication

Sequence similaritiesi

Belongs to the KIN17 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri28 – 50C2H2-typeSequence analysisAdd BLAST23

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG2837 Eukaryota
ENOG410XRJ7 LUCA
GeneTreeiENSGT00390000005903
HOGENOMiHOG000178663
HOVERGENiHBG053068
InParanoidiO60870
KOiK13102
OMAiWLQPNIV
OrthoDBiEOG091G0AQX
PhylomeDBiO60870
TreeFamiTF314393

Family and domain databases

Gene3Di1.10.10.2030, 1 hit
2.30.30.30, 1 hit
InterProiView protein in InterPro
IPR019447 DNA/RNA-bd_Kin17_cons_domain
IPR037321 KIN17-like
IPR038254 KIN17_WH-like_sf
IPR014722 Rib_L2_dom2
IPR036236 Znf_C2H2_sf
PANTHERiPTHR12805 PTHR12805, 1 hit
PfamiView protein in Pfam
PF10357 Kin17_mid, 1 hit
SMARTiView protein in SMART
SM01253 Kin17_mid, 1 hit
SUPFAMiSSF57667 SSF57667, 1 hit
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O60870-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MGKSDFLTPK AIANRIKSKG LQKLRWYCQM CQKQCRDENG FKCHCMSESH
60 70 80 90 100
QRQLLLASEN PQQFMDYFSE EFRNDFLELL RRRFGTKRVH NNIVYNEYIS
110 120 130 140 150
HREHIHMNAT QWETLTDFTK WLGREGLCKV DETPKGWYIQ YIDRDPETIR
160 170 180 190 200
RQLELEKKKK QDLDDEEKTA KFIEEQVRRG LEGKEQEVPT FTELSRENDE
210 220 230 240 250
EKVTFNLSKG ACSSSGATSS KSSTLGPSAL KTIGSSASVK RKESSQSSTQ
260 270 280 290 300
SKEKKKKKSA LDEIMEIEEE KKRTARTDYW LQPEIIVKII TKKLGEKYHK
310 320 330 340 350
KKAIVKEVID KYTAVVKMID SGDKLKLDQT HLETVIPAPG KRILVLNGGY
360 370 380 390
RGNEGTLESI NEKTFSATIV IETGPLKGRR VEGIQYEDIS KLA
Length:393
Mass (Da):45,374
Last modified:January 1, 1999 - v2
Checksum:i515A89B4C8A4C007
GO
Isoform 2 (identifier: O60870-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     374-393: GPLKGRRVEGIQYEDISKLA → V

Note: No experimental confirmation available.
Show »
Length:374
Mass (Da):43,261
Checksum:i4507B25B386B68F7
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
S4R357S4R357_HUMAN
DNA/RNA-binding protein KIN17
KIN
108Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_056074374 – 393GPLKG…ISKLA → V in isoform 2. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005273 mRNA Translation: CAA06462.1
AK301789 mRNA Translation: BAG63244.1
AL158044 Genomic DNA No translation available.
BC017309 mRNA Translation: AAH17309.1
CCDSiCCDS7080.1 [O60870-1]
RefSeqiNP_036443.1, NM_012311.3 [O60870-1]
XP_011517729.1, XM_011519427.1 [O60870-2]
UniGeneiHs.397918

Genome annotation databases

EnsembliENST00000379562; ENSP00000368881; ENSG00000151657 [O60870-1]
GeneIDi22944
KEGGihsa:22944
UCSCiuc001ijt.4 human [O60870-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005273 mRNA Translation: CAA06462.1
AK301789 mRNA Translation: BAG63244.1
AL158044 Genomic DNA No translation available.
BC017309 mRNA Translation: AAH17309.1
CCDSiCCDS7080.1 [O60870-1]
RefSeqiNP_036443.1, NM_012311.3 [O60870-1]
XP_011517729.1, XM_011519427.1 [O60870-2]
UniGeneiHs.397918

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2CKKX-ray1.45A268-393[»]
2V1NNMR-A51-160[»]
ProteinModelPortaliO60870
SMRiO60870
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116600, 13 interactors
CORUMiO60870
IntActiO60870, 3 interactors
MINTiO60870
STRINGi9606.ENSP00000368881

PTM databases

iPTMnetiO60870
PhosphoSitePlusiO60870

Polymorphism and mutation databases

BioMutaiKIN

Proteomic databases

EPDiO60870
MaxQBiO60870
PaxDbiO60870
PeptideAtlasiO60870
PRIDEiO60870
ProteomicsDBi49638

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379562; ENSP00000368881; ENSG00000151657 [O60870-1]
GeneIDi22944
KEGGihsa:22944
UCSCiuc001ijt.4 human [O60870-1]

Organism-specific databases

CTDi22944
DisGeNETi22944
EuPathDBiHostDB:ENSG00000151657.11
GeneCardsiKIN
HGNCiHGNC:6327 KIN
HPAiCAB016339
HPA038700
MIMi601720 gene
neXtProtiNX_O60870
OpenTargetsiENSG00000151657
PharmGKBiPA30113
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2837 Eukaryota
ENOG410XRJ7 LUCA
GeneTreeiENSGT00390000005903
HOGENOMiHOG000178663
HOVERGENiHBG053068
InParanoidiO60870
KOiK13102
OMAiWLQPNIV
OrthoDBiEOG091G0AQX
PhylomeDBiO60870
TreeFamiTF314393

Enzyme and pathway databases

ReactomeiR-HSA-8876725 Protein methylation

Miscellaneous databases

ChiTaRSiKIN human
EvolutionaryTraceiO60870
GeneWikiiKIN_(gene)
GenomeRNAii22944
PROiPR:O60870
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000151657 Expressed in 217 organ(s), highest expression level in lung
CleanExiHS_KIN
ExpressionAtlasiO60870 baseline and differential
GenevisibleiO60870 HS

Family and domain databases

Gene3Di1.10.10.2030, 1 hit
2.30.30.30, 1 hit
InterProiView protein in InterPro
IPR019447 DNA/RNA-bd_Kin17_cons_domain
IPR037321 KIN17-like
IPR038254 KIN17_WH-like_sf
IPR014722 Rib_L2_dom2
IPR036236 Znf_C2H2_sf
PANTHERiPTHR12805 PTHR12805, 1 hit
PfamiView protein in Pfam
PF10357 Kin17_mid, 1 hit
SMARTiView protein in SMART
SM01253 Kin17_mid, 1 hit
SUPFAMiSSF57667 SSF57667, 1 hit
PROSITEiView protein in PROSITE
PS00028 ZINC_FINGER_C2H2_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKIN17_HUMAN
AccessioniPrimary (citable) accession number: O60870
Secondary accession number(s): B4DX32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: January 1, 1999
Last modified: November 7, 2018
This is version 163 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
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