UniProtKB - O60869 (EDF1_HUMAN)
Endothelial differentiation-related factor 1
EDF1
Functioni
Transcriptional coactivator stimulating NR5A1 and ligand-dependent NR1H3/LXRA and PPARG transcriptional activities. Enhances the DNA-binding activity of ATF1, ATF2, CREB1 and NR5A1. Regulates nitric oxid synthase activity probably by sequestering calmodulin in the cytoplasm. May function in endothelial cells differentiation, hormone-induced cardiomyocytes hypertrophy and lipid metabolism.
4 PublicationsRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 92 – 111 | H-T-H motifPROSITE-ProRule annotationAdd BLAST | 20 |
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
- DNA binding Source: UniProtKB-KW
- RNA binding Source: UniProtKB
- TFIID-class transcription factor complex binding Source: UniProtKB
- transcription coactivator activity Source: UniProtKB
GO - Biological processi
- endothelial cell differentiation Source: UniProtKB
- positive regulation of DNA binding Source: UniProtKB
- positive regulation of transcription, DNA-templated Source: UniProtKB
- regulation of lipid metabolic process Source: UniProtKB
- regulation of transcription, DNA-templated Source: UniProtKB
Keywordsi
Molecular function | Activator, Calmodulin-binding, Developmental protein, DNA-binding |
Biological process | Differentiation, Transcription, Transcription regulation |
Enzyme and pathway databases
PathwayCommonsi | O60869 |
SignaLinki | O60869 |
SIGNORi | O60869 |
Names & Taxonomyi
Protein namesi | Recommended name: Endothelial differentiation-related factor 1Short name: EDF-1 Alternative name(s): Multiprotein-bridging factor 1 Short name: MBF1 |
Gene namesi | Name:EDF1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:3164, EDF1 |
MIMi | 605107, gene |
neXtProti | NX_O60869 |
VEuPathDBi | HostDB:ENSG00000107223 |
Subcellular locationi
Cytosol
- cytosol Source: HPA
Nucleus
- nucleolus Source: HPA
- nucleoplasm Source: HPA
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 40 | T → D: Loss of interaction with CALM; when associated with D-58; D-91 and D-111. 1 Publication | 1 | |
Mutagenesisi | 58 | T → D: Loss of interaction with CALM; when associated with D-40; D-91 and D-111. 1 Publication | 1 | |
Mutagenesisi | 65 | T → D: No effect on CALM-binding. No effect; when associated with D-74. 1 Publication | 1 | |
Mutagenesisi | 74 | T → D: No effect on CALM-binding. No effect; when associated with D-65. 1 Publication | 1 | |
Mutagenesisi | 87 | S → A: No effect on CALM-binding. 1 Publication | 1 | |
Mutagenesisi | 87 | S → D: Loss of interaction with CALM and higher affinity for TBP. Same effect; when associated with D-65 and D-74. 1 Publication | 1 | |
Mutagenesisi | 91 | T → A: No effect on CALM-binding. 1 Publication | 1 | |
Mutagenesisi | 91 | T → D: Partial loss of interaction with CALM. Complete loss of interaction; when associated with D-40; D-58 and D-111. 1 Publication | 1 | |
Mutagenesisi | 111 | S → D: Loss of interaction with CALM; when associated with D-40; D-58 and D-91. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 8721 |
OpenTargetsi | ENSG00000107223 |
PharmGKBi | PA27604 |
Miscellaneous databases
Pharosi | O60869, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4295670 |
Genetic variation databases
BioMutai | EDF1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources1 Publication | |||
ChainiPRO_0000149795 | 2 – 148 | Endothelial differentiation-related factor 1Add BLAST | 147 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineCombined sources1 Publication | 1 | |
Modified residuei | 4 | PhosphoserineCombined sources | 1 | |
Modified residuei | 25 | N6-methyllysineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
EPDi | O60869 |
jPOSTi | O60869 |
MassIVEi | O60869 |
MaxQBi | O60869 |
PaxDbi | O60869 |
PeptideAtlasi | O60869 |
PRIDEi | O60869 |
ProteomicsDBi | 49636 [O60869-1] 49637 [O60869-2] |
TopDownProteomicsi | O60869-1 [O60869-1] |
PTM databases
GlyGeni | O60869, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O60869 |
PhosphoSitePlusi | O60869 |
Expressioni
Tissue specificityi
Developmental stagei
Inductioni
Gene expression databases
Bgeei | ENSG00000107223, Expressed in left adrenal gland and 240 other tissues |
Genevisiblei | O60869, HS |
Organism-specific databases
HPAi | ENSG00000107223, Low tissue specificity |
Interactioni
Subunit structurei
Interacts with TBP and the transcription factor IID (TFIID) complex, NR5A2, NR1H3 and PPARG. Interaction with TBP is regulated by phosphorylation. Binds NR5A1, ATF1, FOS and JUN via their conserved basic region. Binding to calmodulin is regulated by calcium and phosphorylation of the IQ motif.
4 PublicationsBinary interactionsi
O60869
Isoform 1 [O60869-1]
With | #Exp. | IntAct |
---|---|---|
TBP [P20226] | 2 | EBI-781310,EBI-355371 |
NR5A1 [Q04752] from Bos taurus. | 4 | EBI-781310,EBI-850837 |
GO - Molecular functioni
- calmodulin binding Source: UniProtKB-KW
- TFIID-class transcription factor complex binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 114260, 58 interactors |
IntActi | O60869, 28 interactors |
MINTi | O60869 |
STRINGi | 9606.ENSP00000224073 |
Miscellaneous databases
RNActi | O60869, protein |
Structurei
Secondary structure
3D structure databases
BMRBi | O60869 |
SMRi | O60869 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O60869 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 81 – 135 | HTH cro/C1-typePROSITE-ProRule annotationAdd BLAST | 55 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 33 – 66 | DisorderedSequence analysisAdd BLAST | 34 | |
Regioni | 37 – 113 | Interaction with NR5A2, PPARG and NR1H31 PublicationAdd BLAST | 77 | |
Regioni | 69 – 108 | Interaction with TBP and NR5A11 PublicationAdd BLAST | 40 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 81 – 88 | IQ motif | 8 |
Domaini
Phylogenomic databases
eggNOGi | KOG3398, Eukaryota |
GeneTreei | ENSGT00390000008519 |
HOGENOMi | CLU_112609_0_1_1 |
InParanoidi | O60869 |
OMAi | NKAHQGT |
OrthoDBi | 1571466at2759 |
PhylomeDBi | O60869 |
TreeFami | TF300064 |
Family and domain databases
CDDi | cd00093, HTH_XRE, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR001387, Cro/C1-type_HTH IPR010982, Lambda_DNA-bd_dom_sf IPR013729, MBF1_N |
Pfami | View protein in Pfam PF01381, HTH_3, 1 hit PF08523, MBF1, 1 hit |
SMARTi | View protein in SMART SM00530, HTH_XRE, 1 hit |
SUPFAMi | SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS50943, HTH_CROC1, 1 hit |
s (3)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAESDWDTVT VLRKKGPTAA QAKSKQAILA AQRRGEDVET SKKWAAGQNK
60 70 80 90 100
QHSITKNTAK LDRETEELHH DRVTLEVGKV IQQGRQSKGL TQKDLATKIN
110 120 130 140
EKPQVIADYE SGRAIPNNQV LGKIERAIGL KLRGKDIGKP IEKGPRAK
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054701 | 129 – 148 | GLKLR…GPRAK → DVGTRSARVLRAQ in isoform 3. CuratedAdd BLAST | 20 | |
Alternative sequenceiVSP_013336 | 130 – 148 | LKLRG…GPRAK → ECPSTLRRVR in isoform 2. 1 PublicationAdd BLAST | 19 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ005259 mRNA Translation: CAA06446.1 AB002282 mRNA Translation: BAA88073.1 AB002283 mRNA Translation: BAA88074.1 CR541914 mRNA Translation: CAG46712.1 BT009863 mRNA Translation: AAP88865.1 AL355987 Genomic DNA No translation available. BC015500 mRNA Translation: AAH15500.1 |
CCDSi | CCDS65193.1 [O60869-3] CCDS7011.1 [O60869-1] CCDS7012.1 [O60869-2] |
RefSeqi | NP_001268226.1, NM_001281297.1 [O60869-3] NP_001268227.1, NM_001281298.1 NP_001268228.1, NM_001281299.1 NP_003783.1, NM_003792.3 [O60869-1] NP_694880.1, NM_153200.2 [O60869-2] |
Genome annotation databases
Ensembli | ENST00000224073; ENSP00000224073; ENSG00000107223 ENST00000371648; ENSP00000360711; ENSG00000107223 [O60869-2] ENST00000371649; ENSP00000360712; ENSG00000107223 [O60869-3] |
GeneIDi | 8721 |
KEGGi | hsa:8721 |
MANE-Selecti | ENST00000224073.6; ENSP00000224073.1; NM_003792.4; NP_003783.1 |
UCSCi | uc004cjt.3, human [O60869-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ005259 mRNA Translation: CAA06446.1 AB002282 mRNA Translation: BAA88073.1 AB002283 mRNA Translation: BAA88074.1 CR541914 mRNA Translation: CAG46712.1 BT009863 mRNA Translation: AAP88865.1 AL355987 Genomic DNA No translation available. BC015500 mRNA Translation: AAH15500.1 |
CCDSi | CCDS65193.1 [O60869-3] CCDS7011.1 [O60869-1] CCDS7012.1 [O60869-2] |
RefSeqi | NP_001268226.1, NM_001281297.1 [O60869-3] NP_001268227.1, NM_001281298.1 NP_001268228.1, NM_001281299.1 NP_003783.1, NM_003792.3 [O60869-1] NP_694880.1, NM_153200.2 [O60869-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1X57 | NMR | - | A | 71-148 | [»] | |
6ZVH | electron microscopy | 2.90 | i | 24-133 | [»] | |
BMRBi | O60869 | |||||
SMRi | O60869 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114260, 58 interactors |
IntActi | O60869, 28 interactors |
MINTi | O60869 |
STRINGi | 9606.ENSP00000224073 |
Chemistry databases
ChEMBLi | CHEMBL4295670 |
PTM databases
GlyGeni | O60869, 1 site, 1 O-linked glycan (1 site) |
iPTMneti | O60869 |
PhosphoSitePlusi | O60869 |
Genetic variation databases
BioMutai | EDF1 |
Proteomic databases
EPDi | O60869 |
jPOSTi | O60869 |
MassIVEi | O60869 |
MaxQBi | O60869 |
PaxDbi | O60869 |
PeptideAtlasi | O60869 |
PRIDEi | O60869 |
ProteomicsDBi | 49636 [O60869-1] 49637 [O60869-2] |
TopDownProteomicsi | O60869-1 [O60869-1] |
Protocols and materials databases
Antibodypediai | 18815, 210 antibodies from 33 providers |
DNASUi | 8721 |
Genome annotation databases
Ensembli | ENST00000224073; ENSP00000224073; ENSG00000107223 ENST00000371648; ENSP00000360711; ENSG00000107223 [O60869-2] ENST00000371649; ENSP00000360712; ENSG00000107223 [O60869-3] |
GeneIDi | 8721 |
KEGGi | hsa:8721 |
MANE-Selecti | ENST00000224073.6; ENSP00000224073.1; NM_003792.4; NP_003783.1 |
UCSCi | uc004cjt.3, human [O60869-1] |
Organism-specific databases
CTDi | 8721 |
DisGeNETi | 8721 |
GeneCardsi | EDF1 |
HGNCi | HGNC:3164, EDF1 |
HPAi | ENSG00000107223, Low tissue specificity |
MIMi | 605107, gene |
neXtProti | NX_O60869 |
OpenTargetsi | ENSG00000107223 |
PharmGKBi | PA27604 |
VEuPathDBi | HostDB:ENSG00000107223 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3398, Eukaryota |
GeneTreei | ENSGT00390000008519 |
HOGENOMi | CLU_112609_0_1_1 |
InParanoidi | O60869 |
OMAi | NKAHQGT |
OrthoDBi | 1571466at2759 |
PhylomeDBi | O60869 |
TreeFami | TF300064 |
Enzyme and pathway databases
PathwayCommonsi | O60869 |
SignaLinki | O60869 |
SIGNORi | O60869 |
Miscellaneous databases
BioGRID-ORCSi | 8721, 79 hits in 1069 CRISPR screens |
ChiTaRSi | EDF1, human |
EvolutionaryTracei | O60869 |
GeneWikii | EDF1 |
GenomeRNAii | 8721 |
Pharosi | O60869, Tbio |
PROi | PR:O60869 |
RNActi | O60869, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000107223, Expressed in left adrenal gland and 240 other tissues |
Genevisiblei | O60869, HS |
Family and domain databases
CDDi | cd00093, HTH_XRE, 1 hit |
Gene3Di | 1.10.260.40, 1 hit |
InterProi | View protein in InterPro IPR001387, Cro/C1-type_HTH IPR010982, Lambda_DNA-bd_dom_sf IPR013729, MBF1_N |
Pfami | View protein in Pfam PF01381, HTH_3, 1 hit PF08523, MBF1, 1 hit |
SMARTi | View protein in SMART SM00530, HTH_XRE, 1 hit |
SUPFAMi | SSF47413, SSF47413, 1 hit |
PROSITEi | View protein in PROSITE PS50943, HTH_CROC1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EDF1_HUMAN | |
Accessioni | O60869Primary (citable) accession number: O60869 Secondary accession number(s): Q5T5T2, Q9UIM1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 12, 2005 |
Last sequence update: | August 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 183 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 9
Human chromosome 9: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references