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Protein

Eukaryotic translation initiation factor 5B

Gene

EIF5B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in translation initiation. Translational GTPase that catalyzes the joining of the 40S and 60S subunits to form the 80S initiation complex with the initiator methionine-tRNA in the P-site base paired to the start codon. GTP binding and hydrolysis induces conformational changes in the enzyme that renders it active for productive interactions with the ribosome. The release of the enzyme after formation of the initiation complex is a prerequisite to form elongation-competent ribosomes.By similarity

Catalytic activityi

GTP + H2O = GDP + phosphate.By similarity

Cofactori

a monovalent cationBy similarityNote: Binds 1 monovalent cation per monomer in the active site. Structural cofactor that stabilizes the GTP-bound "on" state. May also act as a transition state stabilizer of the hydrolysis reaction.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi638 – 645GTPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Initiation factor
Biological processProtein biosynthesis
LigandGTP-binding, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 5B (EC:3.6.5.3)
Short name:
eIF-5B
Alternative name(s):
Translation initiation factor IF-2
Gene namesi
Name:EIF5B
Synonyms:IF2, KIAA0741
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000158417.10
HGNCiHGNC:30793 EIF5B
MIMi606086 gene
neXtProtiNX_O60841

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi640V → G: Loss of activity in vivo. Retains full activity in vitro. 1 Publication1
Mutagenesisi706H → E: Loss of activity; both in vivo and in vitro. 1 Publication1
Mutagenesisi706H → Q: Loss of activity in vivo. Partial activity in vitro. 1 Publication1
Mutagenesisi759D → N: Loss of activity; both in vivo and in vitro. 1 Publication1

Organism-specific databases

OpenTargetsiENSG00000158417
PharmGKBiPA134864457

Polymorphism and mutation databases

BioMutaiEIF5B

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001372941 – 1220Eukaryotic translation initiation factor 5BAdd BLAST1220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei66PhosphoserineCombined sources1
Modified residuei107PhosphoserineCombined sources1
Modified residuei113PhosphoserineCombined sources1
Modified residuei134PhosphotyrosineCombined sources1
Modified residuei135PhosphoserineCombined sources1
Modified residuei137PhosphoserineCombined sources1
Modified residuei164PhosphoserineCombined sources1
Modified residuei171PhosphoserineCombined sources1
Modified residuei182PhosphoserineCombined sources1
Modified residuei183PhosphoserineCombined sources1
Modified residuei186PhosphoserineCombined sources1
Modified residuei190PhosphoserineCombined sources1 Publication1
Modified residuei214PhosphoserineCombined sources1
Modified residuei222PhosphoserineCombined sources1
Modified residuei301PhosphothreonineCombined sources1
Modified residuei438PhosphoserineCombined sources1
Modified residuei498PhosphothreonineCombined sources1
Modified residuei547PhosphoserineCombined sources1
Modified residuei557PhosphoserineBy similarity1
Modified residuei560PhosphoserineCombined sources1
Modified residuei588PhosphoserineCombined sources1
Modified residuei589PhosphoserineCombined sources1
Modified residuei591PhosphoserineCombined sources1
Modified residuei595PhosphoserineCombined sources1
Modified residuei1168PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO60841
MaxQBiO60841
PaxDbiO60841
PeptideAtlasiO60841
PRIDEiO60841
ProteomicsDBi49628

PTM databases

iPTMnetiO60841
PhosphoSitePlusiO60841
SwissPalmiO60841

Miscellaneous databases

PMAP-CutDBiO60841

Expressioni

Gene expression databases

BgeeiENSG00000158417 Expressed in 240 organ(s), highest expression level in tendon of biceps brachii
CleanExiHS_EIF5B
ExpressionAtlasiO60841 baseline and differential
GenevisibleiO60841 HS

Organism-specific databases

HPAiHPA034648
HPA040095

Interactioni

Subunit structurei

Interacts with ANXA5 in a calcium and phospholipid-dependent manner.By similarity

Binary interactionsi

Protein-protein interaction databases

BioGridi115024, 74 interactors
IntActiO60841, 22 interactors
MINTiO60841
STRINGi9606.ENSP00000289371

Structurei

3D structure databases

ProteinModelPortaliO60841
SMRiO60841
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini629 – 846tr-type GPROSITE-ProRule annotationAdd BLAST218

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni638 – 645G1PROSITE-ProRule annotation8
Regioni663 – 667G2PROSITE-ProRule annotation5
Regioni702 – 705G3PROSITE-ProRule annotation4
Regioni756 – 759G4PROSITE-ProRule annotation4
Regioni824 – 826G5PROSITE-ProRule annotation3

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi39 – 50Poly-LysAdd BLAST12
Compositional biasi94 – 99Poly-Lys6
Compositional biasi138 – 142Poly-Asp5
Compositional biasi313 – 322Poly-Lys10
Compositional biasi353 – 356Poly-Glu4
Compositional biasi361 – 364Poly-Glu4
Compositional biasi491 – 496Poly-Glu6
Compositional biasi529 – 567Asp/Glu-rich (acidic)Add BLAST39

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1144 Eukaryota
COG0532 LUCA
GeneTreeiENSGT00900000141098
HOGENOMiHOG000105770
HOVERGENiHBG019036
InParanoidiO60841
KOiK03243
OMAiRHTNVQD
PhylomeDBiO60841
TreeFamiTF101535

Family and domain databases

Gene3Di3.40.50.10050, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR015760 TIF_IF2
IPR023115 TIF_IF2_dom3
IPR036925 TIF_IF2_dom3_sf
IPR009000 Transl_B-barrel_sf
PANTHERiPTHR43381 PTHR43381, 1 hit
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF11987 IF-2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52156 SSF52156, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51722 G_TR_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

O60841-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGKKQKNKSE DSTKDDIDLD ALAAEIEGAG AAKEQEPQKS KGKKKKEKKK
60 70 80 90 100
QDFDEDDILK ELEELSLEAQ GIKADRETVA VKPTENNEEE FTSKDKKKKG
110 120 130 140 150
QKGKKQSFDD NDSEELEDKD SKSKKTAKPK VEMYSGSDDD DDFNKLPKKA
160 170 180 190 200
KGKAQKSNKK WDGSEEDEDN SKKIKERSRI NSSGESGDES DEFLQSRKGQ
210 220 230 240 250
KKNQKNKPGP NIESGNEDDD ASFKIKTVAQ KKAEKKERER KKRDEEKAKL
260 270 280 290 300
RKLKEKEELE TGKKDQSKQK ESQRKFEEET VKSKVTVDTG VIPASEEKAE
310 320 330 340 350
TPTAAEDDNE GDKKKKDKKK KKGEKEEKEK EKKKGPSKAT VKAMQEALAK
360 370 380 390 400
LKEEEERQKR EEEERIKRLE ELEAKRKEEE RLEQEKRERK KQKEKERKER
410 420 430 440 450
LKKEGKLLTK SQREARARAE ATLKLLQAQG VEVPSKDSLP KKRPIYEDKK
460 470 480 490 500
RKKIPQQLES KEVSESMELC AAVEVMEQGV PEKEETPPPV EPEEEEDTED
510 520 530 540 550
AGLDDWEAMA SDEETEKVEG NKVHIEVKEN PEEEEEEEEE EEEDEESEEE
560 570 580 590 600
EEEEGESEGS EGDEEDEKVS DEKDSGKTLD KKPSKEMSSD SEYDSDDDRT
610 620 630 640 650
KEERAYDKAK RRIEKRRLEH SKNVNTEKLR APIICVLGHV DTGKTKILDK
660 670 680 690 700
LRHTHVQDGE AGGITQQIGA TNVPLEAINE QTKMIKNFDR ENVRIPGMLI
710 720 730 740 750
IDTPGHESFS NLRNRGSSLC DIAILVVDIM HGLEPQTIES INLLKSKKCP
760 770 780 790 800
FIVALNKIDR LYDWKKSPDS DVAATLKKQK KNTKDEFEER AKAIIVEFAQ
810 820 830 840 850
QGLNAALFYE NKDPRTFVSL VPTSAHTGDG MGSLIYLLVE LTQTMLSKRL
860 870 880 890 900
AHCEELRAQV MEVKALPGMG TTIDVILING RLKEGDTIIV PGVEGPIVTQ
910 920 930 940 950
IRGLLLPPPM KELRVKNQYE KHKEVEAAQG VKILGKDLEK TLAGLPLLVA
960 970 980 990 1000
YKEDEIPVLK DELIHELKQT LNAIKLEEKG VYVQASTLGS LEALLEFLKT
1010 1020 1030 1040 1050
SEVPYAGINI GPVHKKDVMK ASVMLEHDPQ YAVILAFDVR IERDAQEMAD
1060 1070 1080 1090 1100
SLGVRIFSAE IIYHLFDAFT KYRQDYKKQK QEEFKHIAVF PCKIKILPQY
1110 1120 1130 1140 1150
IFNSRDPIVM GVTVEAGQVK QGTPMCVPSK NFVDIGIVTS IEINHKQVDV
1160 1170 1180 1190 1200
AKKGQEVCVK IEPIPGESPK MFGRHFEATD ILVSKISRQS IDALKDWFRD
1210 1220
EMQKSDWQLI VELKKVFEII
Length:1,220
Mass (Da):138,827
Last modified:November 24, 2009 - v4
Checksum:i454C29FB90AA768E
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A087WUT6A0A087WUT6_HUMAN
Eukaryotic translation initiation f...
EIF5B
1,220Annotation score:

Sequence cautioni

The sequence BAA34461 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti64E → G in BAA34461 (PubMed:9872452).Curated1
Sequence conflicti92T → I in CAB44357 (PubMed:10432305).Curated1
Sequence conflicti180I → M in AAD16006 (PubMed:10200264).Curated1
Sequence conflicti256K → R in AAD16006 (PubMed:10200264).Curated1
Sequence conflicti549E → V in AAD16006 (PubMed:10200264).Curated1
Sequence conflicti669G → W in AAD16006 (PubMed:10200264).Curated1
Sequence conflicti894E → K in CAB44357 (PubMed:10432305).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_055954337S → G. Corresponds to variant dbSNP:rs10642Ensembl.1
Natural variantiVAR_055955360R → G. Corresponds to variant dbSNP:rs3205296Ensembl.1
Natural variantiVAR_060587522K → TCombined sources2 PublicationsCorresponds to variant dbSNP:rs7558074Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006776 mRNA Translation: CAB44357.1
AF078035 mRNA Translation: AAD16006.1
AC018690 Genomic DNA Translation: AAY24313.1
AC079447 Genomic DNA Translation: AAX93258.1
AB018284 mRNA Translation: BAA34461.2 Different initiation.
AL133563 mRNA Translation: CAB63717.1
AJ006412 mRNA Translation: CAA07018.1
CCDSiCCDS42721.1
PIRiT43483
RefSeqiNP_056988.3, NM_015904.3
UniGeneiHs.158688

Genome annotation databases

EnsembliENST00000289371; ENSP00000289371; ENSG00000158417
GeneIDi9669
KEGGihsa:9669
UCSCiuc002tab.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006776 mRNA Translation: CAB44357.1
AF078035 mRNA Translation: AAD16006.1
AC018690 Genomic DNA Translation: AAY24313.1
AC079447 Genomic DNA Translation: AAX93258.1
AB018284 mRNA Translation: BAA34461.2 Different initiation.
AL133563 mRNA Translation: CAB63717.1
AJ006412 mRNA Translation: CAA07018.1
CCDSiCCDS42721.1
PIRiT43483
RefSeqiNP_056988.3, NM_015904.3
UniGeneiHs.158688

3D structure databases

ProteinModelPortaliO60841
SMRiO60841
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115024, 74 interactors
IntActiO60841, 22 interactors
MINTiO60841
STRINGi9606.ENSP00000289371

PTM databases

iPTMnetiO60841
PhosphoSitePlusiO60841
SwissPalmiO60841

Polymorphism and mutation databases

BioMutaiEIF5B

Proteomic databases

EPDiO60841
MaxQBiO60841
PaxDbiO60841
PeptideAtlasiO60841
PRIDEiO60841
ProteomicsDBi49628

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000289371; ENSP00000289371; ENSG00000158417
GeneIDi9669
KEGGihsa:9669
UCSCiuc002tab.4 human

Organism-specific databases

CTDi9669
EuPathDBiHostDB:ENSG00000158417.10
GeneCardsiEIF5B
H-InvDBiHIX0002308
HGNCiHGNC:30793 EIF5B
HPAiHPA034648
HPA040095
MIMi606086 gene
neXtProtiNX_O60841
OpenTargetsiENSG00000158417
PharmGKBiPA134864457
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1144 Eukaryota
COG0532 LUCA
GeneTreeiENSGT00900000141098
HOGENOMiHOG000105770
HOVERGENiHBG019036
InParanoidiO60841
KOiK03243
OMAiRHTNVQD
PhylomeDBiO60841
TreeFamiTF101535

Enzyme and pathway databases

ReactomeiR-HSA-72706 GTP hydrolysis and joining of the 60S ribosomal subunit

Miscellaneous databases

ChiTaRSiEIF5B human
GeneWikiiEIF5B
GenomeRNAii9669
PMAP-CutDBiO60841
PROiPR:O60841
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000158417 Expressed in 240 organ(s), highest expression level in tendon of biceps brachii
CleanExiHS_EIF5B
ExpressionAtlasiO60841 baseline and differential
GenevisibleiO60841 HS

Family and domain databases

Gene3Di3.40.50.10050, 1 hit
InterProiView protein in InterPro
IPR004161 EFTu-like_2
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR000795 TF_GTP-bd_dom
IPR015760 TIF_IF2
IPR023115 TIF_IF2_dom3
IPR036925 TIF_IF2_dom3_sf
IPR009000 Transl_B-barrel_sf
PANTHERiPTHR43381 PTHR43381, 1 hit
PfamiView protein in Pfam
PF00009 GTP_EFTU, 1 hit
PF03144 GTP_EFTU_D2, 1 hit
PF11987 IF-2, 1 hit
PRINTSiPR00315 ELONGATNFCT
SUPFAMiSSF50447 SSF50447, 1 hit
SSF52156 SSF52156, 1 hit
SSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51722 G_TR_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiIF2P_HUMAN
AccessioniPrimary (citable) accession number: O60841
Secondary accession number(s): O95805
, Q53RV7, Q53SI8, Q9UF81, Q9UMN7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 24, 2009
Last modified: November 7, 2018
This is version 182 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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