UniProtKB - O60825 (F262_HUMAN)
Protein
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
Gene
PFKFB2
Organism
Homo sapiens (Human)
Status
Functioni
Synthesis and degradation of fructose 2,6-bisphosphate.
Catalytic activityi
Activity regulationi
Phosphorylation results in the activation of the kinase activity.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 78 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 102 | Fructose 6-phosphateBy similarity | 1 | |
Active sitei | 128 | Sequence analysis | 1 | |
Binding sitei | 130 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 136 | Fructose 6-phosphateBy similarity | 1 | |
Active sitei | 158 | Sequence analysis | 1 | |
Binding sitei | 172 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 193 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 197 | Fructose 6-phosphateBy similarity | 1 | |
Binding sitei | 256 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Sitei | 256 | Transition state stabilizerBy similarity | 1 | |
Active sitei | 257 | Tele-phosphohistidine intermediateBy similarity | 1 | |
Binding sitei | 263 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Sitei | 263 | Transition state stabilizerBy similarity | 1 | |
Binding sitei | 269 | Fructose 2,6-bisphosphate; via amide nitrogenBy similarity | 1 | |
Active sitei | 326 | Proton donor/acceptorBy similarity | 1 | |
Binding sitei | 337 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 351 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 355 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 366 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Sitei | 391 | Transition state stabilizerBy similarity | 1 | |
Binding sitei | 392 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 396 | Fructose 2,6-bisphosphateBy similarity | 1 | |
Binding sitei | 428 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 45 – 53 | ATPBy similarity | 9 | |
Nucleotide bindingi | 167 – 172 | ATPBy similarity | 6 | |
Nucleotide bindingi | 348 – 351 | ATPBy similarity | 4 | |
Nucleotide bindingi | 392 – 396 | ATPBy similarity | 5 |
GO - Molecular functioni
- 6-phosphofructo-2-kinase activity Source: UniProtKB
- ATP binding Source: UniProtKB-KW
- fructose-2,6-bisphosphate 2-phosphatase activity Source: GO_Central
- protein kinase binding Source: UniProtKB
GO - Biological processi
- fructose 2,6-bisphosphate metabolic process Source: GO_Central
- fructose metabolic process Source: ProtInc
- glucose catabolic process Source: Ensembl
- glycolytic process Source: UniProtKB
- lactate metabolic process Source: Ensembl
- positive regulation of glucokinase activity Source: Ensembl
- positive regulation of glycolytic process Source: Reactome
- positive regulation of insulin secretion Source: Ensembl
- response to glucose Source: Ensembl
Keywordsi
Molecular function | Hydrolase, Kinase, Multifunctional enzyme, Transferase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:HS04690-MONOMER |
BRENDAi | 2.7.1.105, 2681 3.1.3.46, 2681 |
PathwayCommonsi | O60825 |
Reactomei | R-HSA-9634600, Regulation of glycolysis by fructose 2,6-bisphosphate metabolism |
SABIO-RKi | O60825 |
SIGNORi | O60825 |
Names & Taxonomyi
Protein namesi | Recommended name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2Short name: 6PF-2-K/Fru-2,6-P2ase 2 Short name: PFK/FBPase 2 Alternative name(s): 6PF-2-K/Fru-2,6-P2ase heart-type isozyme Including the following 2 domains: Fructose-2,6-bisphosphatase (EC:3.1.3.46) |
Gene namesi | Name:PFKFB2 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:8873, PFKFB2 |
MIMi | 171835, gene |
neXtProti | NX_O60825 |
VEuPathDBi | HostDB:ENSG00000123836.14 |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleoplasm Source: HPA
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 466 | S → E: Constitutively active mutant that cannot be phosphorylated and further activated by AMPK. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 5208 |
OpenTargetsi | ENSG00000123836 |
PharmGKBi | PA33212 |
Miscellaneous databases
Pharosi | O60825, Tchem |
Chemistry databases
ChEMBLi | CHEMBL3421525 |
Genetic variation databases
BioMutai | PFKFB2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000179964 | 2 – 505 | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2Add BLAST | 504 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 29 | Phosphoserine; by PKABy similarity | 1 | |
Modified residuei | 466 | Phosphoserine; by AMPKCombined sources1 Publication | 1 | |
Modified residuei | 468 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 475 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 483 | PhosphoserineCombined sources | 1 | |
Modified residuei | 493 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Phosphorylation by AMPK stimulates activity.1 Publication
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | O60825 |
jPOSTi | O60825 |
MassIVEi | O60825 |
MaxQBi | O60825 |
PaxDbi | O60825 |
PeptideAtlasi | O60825 |
PRIDEi | O60825 |
ProteomicsDBi | 49608 [O60825-1] 49609 [O60825-2] |
PTM databases
DEPODi | PFKFB2 |
iPTMneti | O60825 |
PhosphoSitePlusi | O60825 |
Expressioni
Tissue specificityi
Heart.
Gene expression databases
Bgeei | ENSG00000123836, Expressed in islet of Langerhans and 213 other tissues |
ExpressionAtlasi | O60825, baseline and differential |
Genevisiblei | O60825, HS |
Organism-specific databases
HPAi | ENSG00000123836, Low tissue specificity |
Interactioni
Subunit structurei
Homodimer.
By similarityBinary interactionsi
O60825
With | #Exp. | IntAct |
---|---|---|
YWHAZ [P63104] | 2 | EBI-764425,EBI-347088 |
GO - Molecular functioni
- protein kinase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 111229, 34 interactors |
ComplexPortali | CPX-1994, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 complex |
IntActi | O60825, 26 interactors |
MINTi | O60825 |
STRINGi | 9606.ENSP00000356047 |
Chemistry databases
BindingDBi | O60825 |
Miscellaneous databases
RNActi | O60825, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | O60825 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 248 | 6-phosphofructo-2-kinaseAdd BLAST | 247 | |
Regioni | 249 – 505 | Fructose-2,6-bisphosphataseAdd BLAST | 257 |
Sequence similaritiesi
In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated
Phylogenomic databases
eggNOGi | KOG0234, Eukaryota |
GeneTreei | ENSGT00950000182835 |
HOGENOMi | CLU_006383_1_1_1 |
InParanoidi | O60825 |
OMAi | HDSCEWL |
OrthoDBi | 392001at2759 |
PhylomeDBi | O60825 |
TreeFami | TF313541 |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR003094, 6Pfruct_kin IPR013079, 6Phosfructo_kin IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR027417, P-loop_NTPase IPR001345, PG/BPGM_mutase_AS |
PANTHERi | PTHR10606, PTHR10606, 1 hit |
Pfami | View protein in Pfam PF01591, 6PF2K, 1 hit PF00300, His_Phos_1, 1 hit |
PRINTSi | PR00991, 6PFRUCTKNASE |
SMARTi | View protein in SMART SM00855, PGAM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00175, PG_MUTASE, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: O60825-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSGASSSEQN NNSYETKTPN LRMSEKKCSW ASYMTNSPTL IVMIGLPARG
60 70 80 90 100
KTYVSKKLTR YLNWIGVPTK VFNLGVYRRE AVKSYKSYDF FRHDNEEAMK
110 120 130 140 150
IRKQCALVAL EDVKAYLTEE NGQIAVFDAT NTTRERRDMI LNFAEQNSFK
160 170 180 190 200
VFFVESVCDD PDVIAANILE VKVSSPDYPE RNRENVMEDF LKRIECYKVT
210 220 230 240 250
YRPLDPDNYD KDLSFIKVIN VGQRFLVNRV QDYIQSKIVY YLMNIHVQPR
260 270 280 290 300
TIYLCRHGES EFNLLGKIGG DSGLSVRGKQ FAQALRKFLE EQEITDLKVW
310 320 330 340 350
TSQLKRTIQT AESLGVPYEQ WKILNEIDAG VCEEMTYAEI EKRYPEEFAL
360 370 380 390 400
RDQEKYLYRY PGGESYQDLV QRLEPVIMEL ERQGNVLVIS HQAVMRCLLA
410 420 430 440 450
YFLDKGADEL PYLRCPLHTI FKLTPVAYGC KVETIKLNVE AVNTHRDKPT
460 470 480 490 500
NNFPKNQTPV RMRRNSFTPL SSSNTIRRPR NYSVGSRPLK PLSPLRAQDM
QEGAD
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF6RAZ1 | F6RAZ1_HUMAN | 6-phosphofructo-2-kinase/fructose-2... | PFKFB2 | 125 | Annotation score: | ||
F6XNB3 | F6XNB3_HUMAN | 6-phosphofructo-2-kinase/fructose-2... | PFKFB2 | 96 | Annotation score: | ||
A0A087X2H4 | A0A087X2H4_HUMAN | 6-phosphofructo-2-kinase/fructose-2... | PFKFB2 | 49 | Annotation score: | ||
A0A087X278 | A0A087X278_HUMAN | 6-phosphofructo-2-kinase/fructose-2... | PFKFB2 | 44 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 28 | Missing in CAA06605 (PubMed:9652401).Curated | 1 | |
Sequence conflicti | 303 – 304 | QL → HV in CAA06606 (PubMed:9652401).Curated | 2 | |
Sequence conflicti | 372 | R → L in CAA06606 (PubMed:9652401).Curated | 1 | |
Sequence conflicti | 396 | R → H in CAA06606 (PubMed:9652401).Curated | 1 | |
Sequence conflicti | 406 | G → D in CAA06606 (PubMed:9652401).Curated | 1 | |
Sequence conflicti | 427 | A → T in CAA06606 (PubMed:9652401).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_004675 | 451 – 505 | NNFPK…QEGAD → AAETTLAVRRRPSAASLMLP C in isoform 2. 3 PublicationsAdd BLAST | 55 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ005577 Genomic DNA Translation: CAA06605.1 AJ005578 mRNA Translation: CAA06606.1 AB044805 mRNA Translation: BAB19681.1 AF470623 mRNA Translation: AAL99386.1 AK292883 mRNA Translation: BAF85572.1 AL445493 Genomic DNA No translation available. CH471100 Genomic DNA Translation: EAW93508.1 BC069350 mRNA Translation: AAH69350.1 BC069385 mRNA Translation: AAH69385.1 BC069583 mRNA Translation: AAH69583.1 BC069586 mRNA Translation: AAH69586.1 BC069600 mRNA Translation: AAH69600.1 BC075075 mRNA Translation: AAH75075.1 BC075076 mRNA Translation: AAH75076.1 BC112103 mRNA Translation: AAI12104.1 BC112105 mRNA Translation: AAI12106.1 |
CCDSi | CCDS31003.1 [O60825-2] CCDS31004.1 [O60825-1] |
RefSeqi | NP_001018063.1, NM_001018053.1 [O60825-2] NP_006203.2, NM_006212.2 [O60825-1] |
Genome annotation databases
Ensembli | ENST00000367079; ENSP00000356046; ENSG00000123836 [O60825-2] ENST00000367080; ENSP00000356047; ENSG00000123836 [O60825-1] |
GeneIDi | 5208 |
KEGGi | hsa:5208 |
UCSCi | uc001hfg.4, human [O60825-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ005577 Genomic DNA Translation: CAA06605.1 AJ005578 mRNA Translation: CAA06606.1 AB044805 mRNA Translation: BAB19681.1 AF470623 mRNA Translation: AAL99386.1 AK292883 mRNA Translation: BAF85572.1 AL445493 Genomic DNA No translation available. CH471100 Genomic DNA Translation: EAW93508.1 BC069350 mRNA Translation: AAH69350.1 BC069385 mRNA Translation: AAH69385.1 BC069583 mRNA Translation: AAH69583.1 BC069586 mRNA Translation: AAH69586.1 BC069600 mRNA Translation: AAH69600.1 BC075075 mRNA Translation: AAH75075.1 BC075076 mRNA Translation: AAH75076.1 BC112103 mRNA Translation: AAI12104.1 BC112105 mRNA Translation: AAI12106.1 |
CCDSi | CCDS31003.1 [O60825-2] CCDS31004.1 [O60825-1] |
RefSeqi | NP_001018063.1, NM_001018053.1 [O60825-2] NP_006203.2, NM_006212.2 [O60825-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5HTK | X-ray | 2.01 | A/B | 1-505 | [»] | |
SMRi | O60825 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 111229, 34 interactors |
ComplexPortali | CPX-1994, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 complex |
IntActi | O60825, 26 interactors |
MINTi | O60825 |
STRINGi | 9606.ENSP00000356047 |
Chemistry databases
BindingDBi | O60825 |
ChEMBLi | CHEMBL3421525 |
PTM databases
DEPODi | PFKFB2 |
iPTMneti | O60825 |
PhosphoSitePlusi | O60825 |
Genetic variation databases
BioMutai | PFKFB2 |
Proteomic databases
EPDi | O60825 |
jPOSTi | O60825 |
MassIVEi | O60825 |
MaxQBi | O60825 |
PaxDbi | O60825 |
PeptideAtlasi | O60825 |
PRIDEi | O60825 |
ProteomicsDBi | 49608 [O60825-1] 49609 [O60825-2] |
Protocols and materials databases
Antibodypediai | 34592, 417 antibodies |
Genome annotation databases
Ensembli | ENST00000367079; ENSP00000356046; ENSG00000123836 [O60825-2] ENST00000367080; ENSP00000356047; ENSG00000123836 [O60825-1] |
GeneIDi | 5208 |
KEGGi | hsa:5208 |
UCSCi | uc001hfg.4, human [O60825-1] |
Organism-specific databases
CTDi | 5208 |
DisGeNETi | 5208 |
GeneCardsi | PFKFB2 |
HGNCi | HGNC:8873, PFKFB2 |
HPAi | ENSG00000123836, Low tissue specificity |
MIMi | 171835, gene |
neXtProti | NX_O60825 |
OpenTargetsi | ENSG00000123836 |
PharmGKBi | PA33212 |
VEuPathDBi | HostDB:ENSG00000123836.14 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0234, Eukaryota |
GeneTreei | ENSGT00950000182835 |
HOGENOMi | CLU_006383_1_1_1 |
InParanoidi | O60825 |
OMAi | HDSCEWL |
OrthoDBi | 392001at2759 |
PhylomeDBi | O60825 |
TreeFami | TF313541 |
Enzyme and pathway databases
BioCyci | MetaCyc:HS04690-MONOMER |
BRENDAi | 2.7.1.105, 2681 3.1.3.46, 2681 |
PathwayCommonsi | O60825 |
Reactomei | R-HSA-9634600, Regulation of glycolysis by fructose 2,6-bisphosphate metabolism |
SABIO-RKi | O60825 |
SIGNORi | O60825 |
Miscellaneous databases
BioGRID-ORCSi | 5208, 4 hits in 869 CRISPR screens |
ChiTaRSi | PFKFB2, human |
GeneWikii | PFKFB2 |
GenomeRNAii | 5208 |
Pharosi | O60825, Tchem |
PROi | PR:O60825 |
RNActi | O60825, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000123836, Expressed in islet of Langerhans and 213 other tissues |
ExpressionAtlasi | O60825, baseline and differential |
Genevisiblei | O60825, HS |
Family and domain databases
CDDi | cd07067, HP_PGM_like, 1 hit |
Gene3Di | 3.40.50.1240, 1 hit |
InterProi | View protein in InterPro IPR003094, 6Pfruct_kin IPR013079, 6Phosfructo_kin IPR013078, His_Pase_superF_clade-1 IPR029033, His_PPase_superfam IPR027417, P-loop_NTPase IPR001345, PG/BPGM_mutase_AS |
PANTHERi | PTHR10606, PTHR10606, 1 hit |
Pfami | View protein in Pfam PF01591, 6PF2K, 1 hit PF00300, His_Phos_1, 1 hit |
PRINTSi | PR00991, 6PFRUCTKNASE |
SMARTi | View protein in SMART SM00855, PGAM, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit SSF53254, SSF53254, 1 hit |
PROSITEi | View protein in PROSITE PS00175, PG_MUTASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | F262_HUMAN | |
Accessioni | O60825Primary (citable) accession number: O60825 Secondary accession number(s): O60824 Q9H3P1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2001 |
Last sequence update: | January 24, 2001 | |
Last modified: | February 10, 2021 | |
This is version 198 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families