Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2

Gene

PFKFB2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Synthesis and degradation of fructose 2,6-bisphosphate.

Catalytic activityi

Beta-D-fructose 2,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate.
ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Activity regulationi

Phosphorylation results in the activation of the kinase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78Fructose 6-phosphateBy similarity1
Binding sitei102Fructose 6-phosphateBy similarity1
Active sitei128Sequence analysis1
Binding sitei130Fructose 6-phosphateBy similarity1
Binding sitei136Fructose 6-phosphateBy similarity1
Active sitei158Sequence analysis1
Binding sitei172Fructose 6-phosphateBy similarity1
Binding sitei193Fructose 6-phosphateBy similarity1
Binding sitei197Fructose 6-phosphateBy similarity1
Binding sitei256Fructose 2,6-bisphosphateBy similarity1
Sitei256Transition state stabilizerBy similarity1
Active sitei257Tele-phosphohistidine intermediateBy similarity1
Binding sitei263Fructose 2,6-bisphosphateBy similarity1
Sitei263Transition state stabilizerBy similarity1
Binding sitei269Fructose 2,6-bisphosphate; via amide nitrogenBy similarity1
Active sitei326Proton donor/acceptorBy similarity1
Binding sitei337Fructose 2,6-bisphosphateBy similarity1
Binding sitei351Fructose 2,6-bisphosphateBy similarity1
Binding sitei355Fructose 2,6-bisphosphateBy similarity1
Binding sitei366Fructose 2,6-bisphosphateBy similarity1
Sitei391Transition state stabilizerBy similarity1
Binding sitei392Fructose 2,6-bisphosphateBy similarity1
Binding sitei396Fructose 2,6-bisphosphateBy similarity1
Binding sitei428ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 53ATPBy similarity9
Nucleotide bindingi167 – 172ATPBy similarity6
Nucleotide bindingi348 – 351ATPBy similarity4
Nucleotide bindingi392 – 396ATPBy similarity5

GO - Molecular functioni

  • 6-phosphofructo-2-kinase activity Source: UniProtKB
  • ATP binding Source: UniProtKB-KW
  • fructose-2,6-bisphosphate 2-phosphatase activity Source: GO_Central
  • protein kinase binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Kinase, Multifunctional enzyme, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS04690-MONOMER
BRENDAi2.7.1.105 2681
3.1.3.46 2681
ReactomeiR-HSA-70171 Glycolysis
SABIO-RKiO60825
SIGNORiO60825

Names & Taxonomyi

Protein namesi
Recommended name:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
Short name:
6PF-2-K/Fru-2,6-P2ase 2
Short name:
PFK/FBPase 2
Alternative name(s):
6PF-2-K/Fru-2,6-P2ase heart-type isozyme
Including the following 2 domains:
Fructose-2,6-bisphosphatase (EC:3.1.3.46)
Gene namesi
Name:PFKFB2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000123836.14
HGNCiHGNC:8873 PFKFB2
MIMi171835 gene
neXtProtiNX_O60825

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi466S → E: Constitutively active mutant that cannot be phosphorylated and further activated by AMPK. 1 Publication1

Organism-specific databases

DisGeNETi5208
OpenTargetsiENSG00000123836
PharmGKBiPA33212

Chemistry databases

ChEMBLiCHEMBL3421525

Polymorphism and mutation databases

BioMutaiPFKFB2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001799642 – 5056-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2Add BLAST504

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei29Phosphoserine; by PKABy similarity1
Modified residuei466Phosphoserine; by AMPKCombined sources1 Publication1
Modified residuei468PhosphothreonineCombined sources1
Modified residuei475PhosphothreonineCombined sources1
Modified residuei483PhosphoserineCombined sources1
Modified residuei493PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation by AMPK stimulates activity.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO60825
MaxQBiO60825
PaxDbiO60825
PeptideAtlasiO60825
PRIDEiO60825
ProteomicsDBi49608
49609 [O60825-2]

PTM databases

DEPODiO60825
iPTMnetiO60825
PhosphoSitePlusiO60825

Expressioni

Tissue specificityi

Heart.

Gene expression databases

BgeeiENSG00000123836 Expressed in 201 organ(s), highest expression level in right lobe of thyroid gland
CleanExiHS_PFKFB2
ExpressionAtlasiO60825 baseline and differential
GenevisibleiO60825 HS

Organism-specific databases

HPAiHPA049975
HPA063575

Interactioni

Subunit structurei

Homodimer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi111229, 26 interactors
ComplexPortaliCPX-1994 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 complex
IntActiO60825, 21 interactors
MINTiO60825
STRINGi9606.ENSP00000356047

Chemistry databases

BindingDBiO60825

Structurei

Secondary structure

1505
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO60825
SMRiO60825
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 2486-phosphofructo-2-kinaseAdd BLAST247
Regioni249 – 505Fructose-2,6-bisphosphataseAdd BLAST257

Sequence similaritiesi

In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated

Phylogenomic databases

eggNOGiKOG0234 Eukaryota
COG0406 LUCA
GeneTreeiENSGT00390000018751
HOGENOMiHOG000181112
HOVERGENiHBG005628
InParanoidiO60825
KOiK19029
OMAiECGQIAV
OrthoDBiEOG091G0A43
PhylomeDBiO60825
TreeFamiTF313541

Family and domain databases

CDDicd07067 HP_PGM_like, 1 hit
Gene3Di3.40.50.1240, 1 hit
InterProiView protein in InterPro
IPR003094 6Pfruct_kin
IPR013079 6Phosfructo_kin
IPR013078 His_Pase_superF_clade-1
IPR029033 His_PPase_superfam
IPR027417 P-loop_NTPase
IPR001345 PG/BPGM_mutase_AS
PANTHERiPTHR10606 PTHR10606, 1 hit
PfamiView protein in Pfam
PF01591 6PF2K, 1 hit
PF00300 His_Phos_1, 1 hit
PRINTSiPR00991 6PFRUCTKNASE
SMARTiView protein in SMART
SM00855 PGAM, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF53254 SSF53254, 1 hit
PROSITEiView protein in PROSITE
PS00175 PG_MUTASE, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O60825-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGASSSEQN NNSYETKTPN LRMSEKKCSW ASYMTNSPTL IVMIGLPARG
60 70 80 90 100
KTYVSKKLTR YLNWIGVPTK VFNLGVYRRE AVKSYKSYDF FRHDNEEAMK
110 120 130 140 150
IRKQCALVAL EDVKAYLTEE NGQIAVFDAT NTTRERRDMI LNFAEQNSFK
160 170 180 190 200
VFFVESVCDD PDVIAANILE VKVSSPDYPE RNRENVMEDF LKRIECYKVT
210 220 230 240 250
YRPLDPDNYD KDLSFIKVIN VGQRFLVNRV QDYIQSKIVY YLMNIHVQPR
260 270 280 290 300
TIYLCRHGES EFNLLGKIGG DSGLSVRGKQ FAQALRKFLE EQEITDLKVW
310 320 330 340 350
TSQLKRTIQT AESLGVPYEQ WKILNEIDAG VCEEMTYAEI EKRYPEEFAL
360 370 380 390 400
RDQEKYLYRY PGGESYQDLV QRLEPVIMEL ERQGNVLVIS HQAVMRCLLA
410 420 430 440 450
YFLDKGADEL PYLRCPLHTI FKLTPVAYGC KVETIKLNVE AVNTHRDKPT
460 470 480 490 500
NNFPKNQTPV RMRRNSFTPL SSSNTIRRPR NYSVGSRPLK PLSPLRAQDM

QEGAD
Length:505
Mass (Da):58,477
Last modified:January 24, 2001 - v2
Checksum:i5CD6A933A7EBF604
GO
Isoform 2 (identifier: O60825-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     451-505: NNFPKNQTPV...RAQDMQEGAD → AAETTLAVRRRPSAASLMLPC

Show »
Length:471
Mass (Da):54,406
Checksum:i2B9C8957889F4D61
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6XNB3F6XNB3_HUMAN
6-phosphofructo-2-kinase/fructose-2...
PFKFB2
96Annotation score:
F6RAZ1F6RAZ1_HUMAN
6-phosphofructo-2-kinase/fructose-2...
PFKFB2
125Annotation score:
A0A087X2H4A0A087X2H4_HUMAN
6-phosphofructo-2-kinase/fructose-2...
PFKFB2
49Annotation score:
A0A087X278A0A087X278_HUMAN
6-phosphofructo-2-kinase/fructose-2...
PFKFB2
44Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28Missing in CAA06605 (PubMed:9652401).Curated1
Sequence conflicti303 – 304QL → HV in CAA06606 (PubMed:9652401).Curated2
Sequence conflicti372R → L in CAA06606 (PubMed:9652401).Curated1
Sequence conflicti396R → H in CAA06606 (PubMed:9652401).Curated1
Sequence conflicti406G → D in CAA06606 (PubMed:9652401).Curated1
Sequence conflicti427A → T in CAA06606 (PubMed:9652401).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004675451 – 505NNFPK…QEGAD → AAETTLAVRRRPSAASLMLP C in isoform 2. 3 PublicationsAdd BLAST55

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005577 Genomic DNA Translation: CAA06605.1
AJ005578 mRNA Translation: CAA06606.1
AB044805 mRNA Translation: BAB19681.1
AF470623 mRNA Translation: AAL99386.1
AK292883 mRNA Translation: BAF85572.1
AL445493 Genomic DNA No translation available.
CH471100 Genomic DNA Translation: EAW93508.1
BC069350 mRNA Translation: AAH69350.1
BC069385 mRNA Translation: AAH69385.1
BC069583 mRNA Translation: AAH69583.1
BC069586 mRNA Translation: AAH69586.1
BC069600 mRNA Translation: AAH69600.1
BC075075 mRNA Translation: AAH75075.1
BC075076 mRNA Translation: AAH75076.1
BC112103 mRNA Translation: AAI12104.1
BC112105 mRNA Translation: AAI12106.1
CCDSiCCDS31003.1 [O60825-2]
CCDS31004.1 [O60825-1]
RefSeqiNP_001018063.1, NM_001018053.1 [O60825-2]
NP_006203.2, NM_006212.2 [O60825-1]
UniGeneiHs.282702

Genome annotation databases

EnsembliENST00000367079; ENSP00000356046; ENSG00000123836 [O60825-2]
ENST00000367080; ENSP00000356047; ENSG00000123836 [O60825-1]
GeneIDi5208
KEGGihsa:5208
UCSCiuc001hfg.4 human [O60825-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005577 Genomic DNA Translation: CAA06605.1
AJ005578 mRNA Translation: CAA06606.1
AB044805 mRNA Translation: BAB19681.1
AF470623 mRNA Translation: AAL99386.1
AK292883 mRNA Translation: BAF85572.1
AL445493 Genomic DNA No translation available.
CH471100 Genomic DNA Translation: EAW93508.1
BC069350 mRNA Translation: AAH69350.1
BC069385 mRNA Translation: AAH69385.1
BC069583 mRNA Translation: AAH69583.1
BC069586 mRNA Translation: AAH69586.1
BC069600 mRNA Translation: AAH69600.1
BC075075 mRNA Translation: AAH75075.1
BC075076 mRNA Translation: AAH75076.1
BC112103 mRNA Translation: AAI12104.1
BC112105 mRNA Translation: AAI12106.1
CCDSiCCDS31003.1 [O60825-2]
CCDS31004.1 [O60825-1]
RefSeqiNP_001018063.1, NM_001018053.1 [O60825-2]
NP_006203.2, NM_006212.2 [O60825-1]
UniGeneiHs.282702

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5HTKX-ray2.01A/B1-505[»]
ProteinModelPortaliO60825
SMRiO60825
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi111229, 26 interactors
ComplexPortaliCPX-1994 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 complex
IntActiO60825, 21 interactors
MINTiO60825
STRINGi9606.ENSP00000356047

Chemistry databases

BindingDBiO60825
ChEMBLiCHEMBL3421525

PTM databases

DEPODiO60825
iPTMnetiO60825
PhosphoSitePlusiO60825

Polymorphism and mutation databases

BioMutaiPFKFB2

Proteomic databases

EPDiO60825
MaxQBiO60825
PaxDbiO60825
PeptideAtlasiO60825
PRIDEiO60825
ProteomicsDBi49608
49609 [O60825-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000367079; ENSP00000356046; ENSG00000123836 [O60825-2]
ENST00000367080; ENSP00000356047; ENSG00000123836 [O60825-1]
GeneIDi5208
KEGGihsa:5208
UCSCiuc001hfg.4 human [O60825-1]

Organism-specific databases

CTDi5208
DisGeNETi5208
EuPathDBiHostDB:ENSG00000123836.14
GeneCardsiPFKFB2
HGNCiHGNC:8873 PFKFB2
HPAiHPA049975
HPA063575
MIMi171835 gene
neXtProtiNX_O60825
OpenTargetsiENSG00000123836
PharmGKBiPA33212
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0234 Eukaryota
COG0406 LUCA
GeneTreeiENSGT00390000018751
HOGENOMiHOG000181112
HOVERGENiHBG005628
InParanoidiO60825
KOiK19029
OMAiECGQIAV
OrthoDBiEOG091G0A43
PhylomeDBiO60825
TreeFamiTF313541

Enzyme and pathway databases

BioCyciMetaCyc:HS04690-MONOMER
BRENDAi2.7.1.105 2681
3.1.3.46 2681
ReactomeiR-HSA-70171 Glycolysis
SABIO-RKiO60825
SIGNORiO60825

Miscellaneous databases

ChiTaRSiPFKFB2 human
GeneWikiiPFKFB2
GenomeRNAii5208
PROiPR:O60825
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000123836 Expressed in 201 organ(s), highest expression level in right lobe of thyroid gland
CleanExiHS_PFKFB2
ExpressionAtlasiO60825 baseline and differential
GenevisibleiO60825 HS

Family and domain databases

CDDicd07067 HP_PGM_like, 1 hit
Gene3Di3.40.50.1240, 1 hit
InterProiView protein in InterPro
IPR003094 6Pfruct_kin
IPR013079 6Phosfructo_kin
IPR013078 His_Pase_superF_clade-1
IPR029033 His_PPase_superfam
IPR027417 P-loop_NTPase
IPR001345 PG/BPGM_mutase_AS
PANTHERiPTHR10606 PTHR10606, 1 hit
PfamiView protein in Pfam
PF01591 6PF2K, 1 hit
PF00300 His_Phos_1, 1 hit
PRINTSiPR00991 6PFRUCTKNASE
SMARTiView protein in SMART
SM00855 PGAM, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
SSF53254 SSF53254, 1 hit
PROSITEiView protein in PROSITE
PS00175 PG_MUTASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiF262_HUMAN
AccessioniPrimary (citable) accession number: O60825
Secondary accession number(s): O60824
, Q5VVQ3, Q5VVQ4, Q9H3P1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 24, 2001
Last modified: November 7, 2018
This is version 181 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again