PFKFB2

Functionⁱ

Synthesis and degradation of fructose 2,6-bisphosphate.

Catalytic activityⁱ

Beta-D-fructose 2,6-bisphosphate + H₂O = D-fructose 6-phosphate + phosphate.

ATP + D-fructose 6-phosphate = ADP + beta-D-fructose 2,6-bisphosphate.

Activity regulationⁱ

Phosphorylation results in the activation of the kinase activity.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	78	Fructose 6-phosphateBy similarity	1
Binding siteⁱ	102	Fructose 6-phosphateBy similarity	1
Active siteⁱ	128	Sequence analysis	1
Binding siteⁱ	130	Fructose 6-phosphateBy similarity	1
Binding siteⁱ	136	Fructose 6-phosphateBy similarity	1
Active siteⁱ	158	Sequence analysis	1
Binding siteⁱ	172	Fructose 6-phosphateBy similarity	1
Binding siteⁱ	193	Fructose 6-phosphateBy similarity	1
Binding siteⁱ	197	Fructose 6-phosphateBy similarity	1
Binding siteⁱ	256	Fructose 2,6-bisphosphateBy similarity	1
Siteⁱ	256	Transition state stabilizerBy similarity	1
Active siteⁱ	257	Tele-phosphohistidine intermediateBy similarity	1
Binding siteⁱ	263	Fructose 2,6-bisphosphateBy similarity	1
Siteⁱ	263	Transition state stabilizerBy similarity	1
Binding siteⁱ	269	Fructose 2,6-bisphosphate; via amide nitrogenBy similarity	1
Active siteⁱ	326	Proton donor/acceptorBy similarity	1
Binding siteⁱ	337	Fructose 2,6-bisphosphateBy similarity	1
Binding siteⁱ	351	Fructose 2,6-bisphosphateBy similarity	1
Binding siteⁱ	355	Fructose 2,6-bisphosphateBy similarity	1
Binding siteⁱ	366	Fructose 2,6-bisphosphateBy similarity	1
Siteⁱ	391	Transition state stabilizerBy similarity	1
Binding siteⁱ	392	Fructose 2,6-bisphosphateBy similarity	1
Binding siteⁱ	396	Fructose 2,6-bisphosphateBy similarity	1
Binding siteⁱ	428	ATPBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Length
Nucleotide bindingⁱ	45 – 53	ATPBy similarity	9
Nucleotide bindingⁱ	167 – 172	ATPBy similarity	6
Nucleotide bindingⁱ	348 – 351	ATPBy similarity	4
Nucleotide bindingⁱ	392 – 396	ATPBy similarity	5

GO - Molecular functionⁱ

6-phosphofructo-2-kinase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 11069105
ATP binding Source: UniProtKB-KW
fructose-2,6-bisphosphate 2-phosphatase activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein kinase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 11069105

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

fructose 2,6-bisphosphate metabolic process
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
fructose metabolic process
Source: ProtInc
Traceable author statementⁱ
- 9652401
glucose catabolic process Source: Ensembl
glycolytic process
Source: UniProtKB
Inferred from direct assayⁱ
- 11069105
lactate metabolic process Source: Ensembl
positive regulation of glucokinase activity Source: Ensembl
positive regulation of glycolytic process Source: Reactome
positive regulation of insulin secretion Source: Ensembl
response to glucose Source: Ensembl

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Kinase, Multifunctional enzyme, Transferase
Ligand	ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCycⁱ	MetaCyc:HS04690-MONOMER
BRENDAⁱ	2.7.1.105 2681 3.1.3.46 2681
Reactomeⁱ	R-HSA-70171 Glycolysis
SABIO-RKⁱ	O60825
SIGNORⁱ	O60825

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 Short name: 6PF-2-K/Fru-2,6-P2ase 2 Short name: PFK/FBPase 2 Alternative name(s): 6PF-2-K/Fru-2,6-P2ase heart-type isozyme Including the following 2 domains: 6-phosphofructo-2-kinase (EC:2.7.1.105) Fructose-2,6-bisphosphatase (EC:3.1.3.46)
Gene namesⁱ	Name:PFKFB2
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 1

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000123836.14
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:8873 PFKFB2
MIMⁱ	171835 gene
neXtProtⁱ	NX_O60825

Subcellular locationⁱ

GO - Cellular component

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	466	S → E: Constitutively active mutant that cannot be phosphorylated and further activated by AMPK. 1 Publication		1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	5208
Open Targets More...OpenTargetsⁱ	ENSG00000123836
PharmGKBⁱ	PA33212

Chemistry databases

ChEMBLⁱ	CHEMBL3421525

ChEMBLⁱ

CHEMBL3421525

Polymorphism and mutation databases

BioMutaⁱ	PFKFB2

BioMutaⁱ

PFKFB2

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Initiator methionineⁱ		RemovedCombined sources
ChainⁱPRO_0000179964	2 – 505	6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2Add BLAST		504

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	2	N-acetylserineCombined sources	1
Modified residueⁱ	29	Phosphoserine; by PKABy similarity	1
Modified residueⁱ	466	Phosphoserine; by AMPKCombined sources1 Publication	1
Modified residueⁱ	468	PhosphothreonineCombined sources	1
Modified residueⁱ	475	PhosphothreonineCombined sources	1
Modified residueⁱ	483	PhosphoserineCombined sources	1
Modified residueⁱ	493	PhosphoserineCombined sources	1

Post-translational modificationⁱ

Phosphorylation by AMPK stimulates activity.1 Publication

Keywords - PTMⁱ

Acetylation, Phosphoprotein

Proteomic databases

EPDⁱ	O60825
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O60825
PaxDbⁱ	O60825
PeptideAtlas More...PeptideAtlasⁱ	O60825
PRIDEⁱ	O60825
ProteomicsDBⁱ	49608 49609 [O60825-2]

PTM databases

DEPODⁱ	O60825
iPTMnetⁱ	O60825
PhosphoSitePlusⁱ	O60825

Expressionⁱ

Tissue specificityⁱ

Heart.

Gene expression databases

Bgeeⁱ	ENSG00000123836 Expressed in 201 organ(s), highest expression level in right lobe of thyroid gland
CleanExⁱ	HS_PFKFB2
ExpressionAtlasⁱ	O60825 baseline and differential
Genevisibleⁱ	O60825 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA049975 HPA063575

HPAⁱ

HPA049975
HPA063575

Interactionⁱ

Subunit structureⁱ

Homodimer.By similarity

GO - Molecular functionⁱ

protein kinase binding
Source: UniProtKB
Inferred from physical interactionⁱ
- 11069105

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	111229, 26 interactors
ComplexPortalⁱ	CPX-1994 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 complex
IntActⁱ	O60825, 21 interactors
Molecular INTeraction database More...MINTⁱ	O60825
STRINGⁱ	9606.ENSP00000356047

Chemistry databases

BindingDBⁱ

O60825

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Beta strandⁱ	39 – 44	Combined sources	6
Beta strandⁱ	47 – 50	Combined sources	4
Helixⁱ	51 – 64	Combined sources	14
Beta strandⁱ	69 – 73	Combined sources	5
Helixⁱ	74 – 82	Combined sources	9
Helixⁱ	88 – 91	Combined sources	4
Helixⁱ	96 – 119	Combined sources	24
Beta strandⁱ	123 – 129	Combined sources	7
Helixⁱ	134 – 147	Combined sources	14
Beta strandⁱ	150 – 157	Combined sources	8
Helixⁱ	161 – 174	Combined sources	14
Helixⁱ	186 – 200	Combined sources	15
Turnⁱ	206 – 212	Combined sources	7
Beta strandⁱ	215 – 219	Combined sources	5
Turnⁱ	220 – 223	Combined sources	4
Beta strandⁱ	224 – 228	Combined sources	5
Helixⁱ	233 – 242	Combined sources	10
Beta strandⁱ	252 – 256	Combined sources	5
Helixⁱ	261 – 265	Combined sources	5
Helixⁱ	276 – 292	Combined sources	17
Beta strandⁱ	298 – 301	Combined sources	4
Helixⁱ	305 – 312	Combined sources	8
Helixⁱ	322 – 324	Combined sources	3
Helixⁱ	330 – 332	Combined sources	3
Helixⁱ	337 – 343	Combined sources	7
Helixⁱ	345 – 353	Combined sources	9
Turnⁱ	355 – 357	Combined sources	3
Helixⁱ	366 – 382	Combined sources	17
Beta strandⁱ	384 – 390	Combined sources	7
Helixⁱ	392 – 402	Combined sources	11
Turnⁱ	407 – 409	Combined sources	3
Helixⁱ	410 – 412	Combined sources	3
Beta strandⁱ	419 – 426	Combined sources	8
Beta strandⁱ	429 – 436	Combined sources	8

Show more details

3D structure databases

ProteinModelPortalⁱ	O60825
SMRⁱ	O60825
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	2 – 248	6-phosphofructo-2-kinaseAdd BLAST		247
Regionⁱ	249 – 505	Fructose-2,6-bisphosphataseAdd BLAST		257

Sequence similaritiesⁱ

In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0234 Eukaryota COG0406 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000018751
HOGENOMⁱ	HOG000181112
HOVERGENⁱ	HBG005628
InParanoidⁱ	O60825
KEGG Orthology (KO) More...KOⁱ	K19029
OMAⁱ	ECGQIAV
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0A43
PhylomeDBⁱ	O60825
TreeFamⁱ	TF313541

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd07067 HP_PGM_like, 1 hit
Gene3Dⁱ	3.40.50.1240, 1 hit
InterProⁱ	View protein in InterPro IPR003094 6Pfruct_kin IPR013079 6Phosfructo_kin IPR013078 His_Pase_superF_clade-1 IPR029033 His_PPase_superfam IPR027417 P-loop_NTPase IPR001345 PG/BPGM_mutase_AS
PANTHERⁱ	PTHR10606 PTHR10606, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01591 6PF2K, 1 hit PF00300 His_Phos_1, 1 hit
PRINTSⁱ	PR00991 6PFRUCTKNASE
SMARTⁱ	View protein in SMART SM00855 PGAM, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit SSF53254 SSF53254, 1 hit
PROSITEⁱ	View protein in PROSITE PS00175 PG_MUTASE, 1 hit

Sequences (2+)ⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show all Align All

Isoform 1 (identifier: O60825-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSGASSSEQN NNSYETKTPN LRMSEKKCSW ASYMTNSPTL IVMIGLPARG 
        60         70         80         90        100
KTYVSKKLTR YLNWIGVPTK VFNLGVYRRE AVKSYKSYDF FRHDNEEAMK 
       110        120        130        140        150
IRKQCALVAL EDVKAYLTEE NGQIAVFDAT NTTRERRDMI LNFAEQNSFK 
       160        170        180        190        200
VFFVESVCDD PDVIAANILE VKVSSPDYPE RNRENVMEDF LKRIECYKVT 
       210        220        230        240        250
YRPLDPDNYD KDLSFIKVIN VGQRFLVNRV QDYIQSKIVY YLMNIHVQPR 
       260        270        280        290        300
TIYLCRHGES EFNLLGKIGG DSGLSVRGKQ FAQALRKFLE EQEITDLKVW 
       310        320        330        340        350
TSQLKRTIQT AESLGVPYEQ WKILNEIDAG VCEEMTYAEI EKRYPEEFAL 
       360        370        380        390        400
RDQEKYLYRY PGGESYQDLV QRLEPVIMEL ERQGNVLVIS HQAVMRCLLA 
       410        420        430        440        450
YFLDKGADEL PYLRCPLHTI FKLTPVAYGC KVETIKLNVE AVNTHRDKPT 
       460        470        480        490        500
NNFPKNQTPV RMRRNSFTPL SSSNTIRRPR NYSVGSRPLK PLSPLRAQDM 

QEGAD

Length:505

Mass (Da):58,477

Last modified:January 24, 2001 - v2

Checksum:ⁱ5CD6A933A7EBF604

GO

Isoform 2 (identifier: O60825-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
451-505: NNFPKNQTPV...RAQDMQEGAD → AAETTLAVRRRPSAASLMLPC

« Hide

        10         20         30         40         50
MSGASSSEQN NNSYETKTPN LRMSEKKCSW ASYMTNSPTL IVMIGLPARG 
        60         70         80         90        100
KTYVSKKLTR YLNWIGVPTK VFNLGVYRRE AVKSYKSYDF FRHDNEEAMK 
       110        120        130        140        150
IRKQCALVAL EDVKAYLTEE NGQIAVFDAT NTTRERRDMI LNFAEQNSFK 
       160        170        180        190        200
VFFVESVCDD PDVIAANILE VKVSSPDYPE RNRENVMEDF LKRIECYKVT 
       210        220        230        240        250
YRPLDPDNYD KDLSFIKVIN VGQRFLVNRV QDYIQSKIVY YLMNIHVQPR 
       260        270        280        290        300
TIYLCRHGES EFNLLGKIGG DSGLSVRGKQ FAQALRKFLE EQEITDLKVW 
       310        320        330        340        350
TSQLKRTIQT AESLGVPYEQ WKILNEIDAG VCEEMTYAEI EKRYPEEFAL 
       360        370        380        390        400
RDQEKYLYRY PGGESYQDLV QRLEPVIMEL ERQGNVLVIS HQAVMRCLLA 
       410        420        430        440        450
YFLDKGADEL PYLRCPLHTI FKLTPVAYGC KVETIKLNVE AVNTHRDKPT 
       460        470 
AAETTLAVRR RPSAASLMLP C

Show »

Length:471

Mass (Da):54,406

Checksum:ⁱ2B9C8957889F4D61

GO

Computationally mapped potential isoform sequencesⁱ

There are 4 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

F6XNB3	F6XNB3_HUMAN	6-phosphofructo-2-kinase/fructose-2... 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2	PFKFB2	96	Annotation score:
F6RAZ1	F6RAZ1_HUMAN	6-phosphofructo-2-kinase/fructose-2... 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2	PFKFB2	125	Annotation score:
A0A087X2H4	A0A087X2H4_HUMAN	6-phosphofructo-2-kinase/fructose-2... 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2	PFKFB2	49	Annotation score:
A0A087X278	A0A087X278_HUMAN	6-phosphofructo-2-kinase/fructose-2... 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2	PFKFB2	44	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	28	Missing in CAA06605 (PubMed:9652401).Curated	1
Sequence conflictⁱ	303 – 304	QL → HV in CAA06606 (PubMed:9652401).Curated	2
Sequence conflictⁱ	372	R → L in CAA06606 (PubMed:9652401).Curated	1
Sequence conflictⁱ	396	R → H in CAA06606 (PubMed:9652401).Curated	1
Sequence conflictⁱ	406	G → D in CAA06606 (PubMed:9652401).Curated	1
Sequence conflictⁱ	427	A → T in CAA06606 (PubMed:9652401).Curated	1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Alternative sequenceⁱVSP_004675	451 – 505	NNFPK…QEGAD → AAETTLAVRRRPSAASLMLP C in isoform 2. 3 PublicationsAdd BLAST		55

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ005577 Genomic DNA Translation: CAA06605.1 AJ005578 mRNA Translation: CAA06606.1 AB044805 mRNA Translation: BAB19681.1 AF470623 mRNA Translation: AAL99386.1 AK292883 mRNA Translation: BAF85572.1 AL445493 Genomic DNA No translation available. CH471100 Genomic DNA Translation: EAW93508.1 BC069350 mRNA Translation: AAH69350.1 BC069385 mRNA Translation: AAH69385.1 BC069583 mRNA Translation: AAH69583.1 BC069586 mRNA Translation: AAH69586.1 BC069600 mRNA Translation: AAH69600.1 BC075075 mRNA Translation: AAH75075.1 BC075076 mRNA Translation: AAH75076.1 BC112103 mRNA Translation: AAI12104.1 BC112105 mRNA Translation: AAI12106.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS31003.1 [O60825-2] CCDS31004.1 [O60825-1]
NCBI Reference Sequences More...RefSeqⁱ	NP_001018063.1, NM_001018053.1 [O60825-2] NP_006203.2, NM_006212.2 [O60825-1]
UniGeneⁱ	Hs.282702

Genome annotation databases

Ensemblⁱ	ENST00000367079; ENSP00000356046; ENSG00000123836 [O60825-2] ENST00000367080; ENSP00000356047; ENSG00000123836 [O60825-1]
GeneIDⁱ	5208
KEGGⁱ	hsa:5208
UCSC genome browser More...UCSCⁱ	uc001hfg.4 human [O60825-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing

Protein	Similar proteins		Species	Score	Length	Source
O60825	6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2		PANTR		505	UniRef100_O60825
Uncharacterized protein		PANPA		505
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2		GORGO		505
6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 transcript variant 3		HUMAN		472
UPI000387B6EB		HUMAN		263
+1
O60825-2	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2		PANTR		471	UniRef100_A0A2I3T371
Uncharacterized protein		PANPA		471
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2		GORGO		471
cDNA FLJ53941, highly similar to 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2		HUMAN		373

Protein	Similar proteins		Species	Score	Length	Source
O60825	6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2		PANTR		505	UniRef90_O60825
Uncharacterized protein		PANPA		505
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2		GORGO		505
6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 transcript variant 3		HUMAN		472
Uncharacterized protein		PIG		535
+162
O60825-2	6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2		BOVIN		471	UniRef90_A6QL99
Uncharacterized protein		Cebus capucinus imitator		482
Uncharacterized protein		AOTNA		474
6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 isoform X2		MESAU		474
Uncharacterized protein		PROCO		474
+97

Protein	Similar proteins		Species	Score	Length	Source
O60825	6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3	)	HUMAN		520	UniRef50_O60825
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3		PONAB		514
6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 2		PANTR		505
Uncharacterized protein		PANPA		505
6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2		GORGO		505
+708
O60825-2	6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3		RAT		555	UniRef50_O35552
6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X1		Enhydra lutris kenyoni		555
6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3 splice variant 2		MOUSE		555
6-phosphofructo-2-kinase/fructose-2, 6-biphosphatase 3-like isoform 6		CRIGR		555
6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 3 isoform X1		MESAU		555
+692

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AJ005577 Genomic DNA Translation: CAA06605.1 AJ005578 mRNA Translation: CAA06606.1 AB044805 mRNA Translation: BAB19681.1 AF470623 mRNA Translation: AAL99386.1 AK292883 mRNA Translation: BAF85572.1 AL445493 Genomic DNA No translation available. CH471100 Genomic DNA Translation: EAW93508.1 BC069350 mRNA Translation: AAH69350.1 BC069385 mRNA Translation: AAH69385.1 BC069583 mRNA Translation: AAH69583.1 BC069586 mRNA Translation: AAH69586.1 BC069600 mRNA Translation: AAH69600.1 BC075075 mRNA Translation: AAH75075.1 BC075076 mRNA Translation: AAH75076.1 BC112103 mRNA Translation: AAI12104.1 BC112105 mRNA Translation: AAI12106.1
CCDSⁱ	CCDS31003.1 [O60825-2] CCDS31004.1 [O60825-1]
RefSeqⁱ	NP_001018063.1, NM_001018053.1 [O60825-2] NP_006203.2, NM_006212.2 [O60825-1]
UniGeneⁱ	Hs.282702

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	5HTK	X-ray	2.01	A/B	1-505	[»]
ProteinModelPortalⁱ	O60825
SMRⁱ	O60825
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	111229, 26 interactors
ComplexPortalⁱ	CPX-1994 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 complex
IntActⁱ	O60825, 21 interactors
MINTⁱ	O60825
STRINGⁱ	9606.ENSP00000356047

Chemistry databases

BindingDBⁱ	O60825
ChEMBLⁱ	CHEMBL3421525

PTM databases

DEPODⁱ	O60825
iPTMnetⁱ	O60825
PhosphoSitePlusⁱ	O60825

Polymorphism and mutation databases

BioMutaⁱ	PFKFB2

BioMutaⁱ

PFKFB2

Proteomic databases

EPDⁱ	O60825
MaxQBⁱ	O60825
PaxDbⁱ	O60825
PeptideAtlasⁱ	O60825
PRIDEⁱ	O60825
ProteomicsDBⁱ	49608 49609 [O60825-2]

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENST00000367079; ENSP00000356046; ENSG00000123836 [O60825-2] ENST00000367080; ENSP00000356047; ENSG00000123836 [O60825-1]
GeneIDⁱ	5208
KEGGⁱ	hsa:5208
UCSCⁱ	uc001hfg.4 human [O60825-1]

Organism-specific databases

CTDⁱ	5208
DisGeNETⁱ	5208
EuPathDBⁱ	HostDB:ENSG00000123836.14
GeneCardsⁱ	PFKFB2
HGNCⁱ	HGNC:8873 PFKFB2
HPAⁱ	HPA049975 HPA063575
MIMⁱ	171835 gene
neXtProtⁱ	NX_O60825
OpenTargetsⁱ	ENSG00000123836
PharmGKBⁱ	PA33212
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG0234 Eukaryota COG0406 LUCA
GeneTreeⁱ	ENSGT00390000018751
HOGENOMⁱ	HOG000181112
HOVERGENⁱ	HBG005628
InParanoidⁱ	O60825
KOⁱ	K19029
OMAⁱ	ECGQIAV
OrthoDBⁱ	EOG091G0A43
PhylomeDBⁱ	O60825
TreeFamⁱ	TF313541

Enzyme and pathway databases

BioCycⁱ	MetaCyc:HS04690-MONOMER
BRENDAⁱ	2.7.1.105 2681 3.1.3.46 2681
Reactomeⁱ	R-HSA-70171 Glycolysis
SABIO-RKⁱ	O60825
SIGNORⁱ	O60825

Miscellaneous databases

ChiTaRSⁱ	PFKFB2 human
GeneWikiⁱ	PFKFB2
GenomeRNAiⁱ	5208
Protein Ontology More...PROⁱ	PR:O60825
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000123836 Expressed in 201 organ(s), highest expression level in right lobe of thyroid gland
CleanExⁱ	HS_PFKFB2
ExpressionAtlasⁱ	O60825 baseline and differential
Genevisibleⁱ	O60825 HS

Family and domain databases

CDDⁱ	cd07067 HP_PGM_like, 1 hit
Gene3Dⁱ	3.40.50.1240, 1 hit
InterProⁱ	View protein in InterPro IPR003094 6Pfruct_kin IPR013079 6Phosfructo_kin IPR013078 His_Pase_superF_clade-1 IPR029033 His_PPase_superfam IPR027417 P-loop_NTPase IPR001345 PG/BPGM_mutase_AS
PANTHERⁱ	PTHR10606 PTHR10606, 1 hit
Pfamⁱ	View protein in Pfam PF01591 6PF2K, 1 hit PF00300 His_Phos_1, 1 hit
PRINTSⁱ	PR00991 6PFRUCTKNASE
SMARTⁱ	View protein in SMART SM00855 PGAM, 1 hit
SUPFAMⁱ	SSF52540 SSF52540, 1 hit SSF53254 SSF53254, 1 hit
PROSITEⁱ	View protein in PROSITE PS00175 PG_MUTASE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	F262_HUMAN
Accessionⁱ	O60825Primary (citable) accession number: O60825 Secondary accession number(s): O60824 , Q5VVQ3, Q5VVQ4, Q9H3P1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
	Last sequence update:	January 24, 2001
	Last modified:	November 7, 2018
	This is version 181 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references

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UniProtKB - O60825 (F262_HUMAN)

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6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2

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GO - Molecular functionⁱ

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Keywords - PTMⁱ

Expressionⁱ

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Keywords - Coding sequence diversityⁱ

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Keywords - Technical termⁱ