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UniProtKB - O60825 (F262_HUMAN)

Entry version 195 (12 Aug 2020)
Sequence version 2 (24 Jan 2001)
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Protein

6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2

Gene

PFKFB2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Synthesis and degradation of fructose 2,6-bisphosphate.

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Phosphorylation results in the activation of the kinase activity.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei78Fructose 6-phosphateBy similarity1
Binding sitei102Fructose 6-phosphateBy similarity1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei128Sequence analysis1
Binding sitei130Fructose 6-phosphateBy similarity1
Binding sitei136Fructose 6-phosphateBy similarity1
Active sitei158Sequence analysis1
Binding sitei172Fructose 6-phosphateBy similarity1
Binding sitei193Fructose 6-phosphateBy similarity1
Binding sitei197Fructose 6-phosphateBy similarity1
Binding sitei256Fructose 2,6-bisphosphateBy similarity1
<p>This subsection describes interesting single amino acid sites on the sequence that are not defined in any other subsection. This subsection can be displayed in different sections ('Function', 'PTM / Processing', 'Pathology and Biotech') according to its content.<p><a href='/help/site' target='_top'>More...</a></p>Sitei256Transition state stabilizerBy similarity1
Active sitei257Tele-phosphohistidine intermediateBy similarity1
Binding sitei263Fructose 2,6-bisphosphateBy similarity1
Sitei263Transition state stabilizerBy similarity1
Binding sitei269Fructose 2,6-bisphosphate; via amide nitrogenBy similarity1
Active sitei326Proton donor/acceptorBy similarity1
Binding sitei337Fructose 2,6-bisphosphateBy similarity1
Binding sitei351Fructose 2,6-bisphosphateBy similarity1
Binding sitei355Fructose 2,6-bisphosphateBy similarity1
Binding sitei366Fructose 2,6-bisphosphateBy similarity1
Sitei391Transition state stabilizerBy similarity1
Binding sitei392Fructose 2,6-bisphosphateBy similarity1
Binding sitei396Fructose 2,6-bisphosphateBy similarity1
Binding sitei428ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi45 – 53ATPBy similarity9
Nucleotide bindingi167 – 172ATPBy similarity6
Nucleotide bindingi348 – 351ATPBy similarity4
Nucleotide bindingi392 – 396ATPBy similarity5

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHydrolase, Kinase, Multifunctional enzyme, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...BioCyci		MetaCyc:HS04690-MONOMER

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.7.1.105, 2681
3.1.3.46, 2681

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		O60825

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-9634600, Regulation of glycolysis by fructose 2,6-bisphosphate metabolism

SABIO-RK: Biochemical Reaction Kinetics Database

More...SABIO-RKi		O60825

SIGNOR Signaling Network Open Resource

More...SIGNORi		O60825

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2
Short name:
6PF-2-K/Fru-2,6-P2ase 2
Short name:
PFK/FBPase 2
Alternative name(s):
6PF-2-K/Fru-2,6-P2ase heart-type isozyme
Including the following 2 domains:
6-phosphofructo-2-kinase (EC:2.7.1.105)
Fructose-2,6-bisphosphatase (EC:3.1.3.46)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:PFKFB2
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 1

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...EuPathDBi		HostDB:ENSG00000123836.14

Human Gene Nomenclature Database

More...HGNCi		HGNC:8873, PFKFB2

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		171835, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_O60825

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi466S → E: Constitutively active mutant that cannot be phosphorylated and further activated by AMPK. 1 Publication1

Organism-specific databases

DisGeNET

More...DisGeNETi		5208

Open Targets

More...OpenTargetsi		ENSG00000123836

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA33212

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		O60825, Tchem

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL3421525

Polymorphism and mutation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		PFKFB2

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM / Processing</a> section indicates that the initiator methionine is cleaved from the mature protein.<p><a href='/help/init_met' target='_top'>More...</a></p>Initiator methionineiRemovedCombined sources
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001799642 – 5056-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2Add BLAST504

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei2N-acetylserineCombined sources1
Modified residuei29Phosphoserine; by PKABy similarity1
Modified residuei466Phosphoserine; by AMPKCombined sources1 Publication1
Modified residuei468PhosphothreonineCombined sources1
Modified residuei475PhosphothreonineCombined sources1
Modified residuei483PhosphoserineCombined sources1
Modified residuei493PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylation by AMPK stimulates activity.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		O60825

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		O60825

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		O60825

MaxQB - The MaxQuant DataBase

More...MaxQBi		O60825

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		O60825

PeptideAtlas

More...PeptideAtlasi		O60825

PRoteomics IDEntifications database

More...PRIDEi		O60825

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		49608 [O60825-1]
49609 [O60825-2]

PTM databases

DEPOD human dephosphorylation database

More...DEPODi		PFKFB2

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		O60825

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		O60825

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Heart.

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000123836, Expressed in islet of Langerhans and 213 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		O60825, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		O60825, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000123836, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Homodimer.

By similarity

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		111229, 31 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...ComplexPortali		CPX-1994, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 complex

Protein interaction database and analysis system

More...IntActi		O60825, 25 interactors

Molecular INTeraction database

More...MINTi		O60825

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000356047

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		O60825

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		O60825, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1505
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		O60825

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni2 – 2486-phosphofructo-2-kinaseAdd BLAST247
Regioni249 – 505Fructose-2,6-bisphosphataseAdd BLAST257

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

In the C-terminal section; belongs to the phosphoglycerate mutase family.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG0234, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00950000182835

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_006383_1_1_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		O60825

KEGG Orthology (KO)

More...KOi		K19029

Identification of Orthologs from Complete Genome Data

More...OMAi		HDSCEWL

Database of Orthologous Groups

More...OrthoDBi		392001at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		O60825

TreeFam database of animal gene trees

More...TreeFami		TF313541

Family and domain databases

Conserved Domains Database

More...CDDi		cd07067, HP_PGM_like, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.40.50.1240, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR003094, 6Pfruct_kin
IPR013079, 6Phosfructo_kin
IPR013078, His_Pase_superF_clade-1
IPR029033, His_PPase_superfam
IPR027417, P-loop_NTPase
IPR001345, PG/BPGM_mutase_AS

The PANTHER Classification System

More...PANTHERi		PTHR10606, PTHR10606, 1 hit

Pfam protein domain database

More...Pfami		View protein in Pfam
PF01591, 6PF2K, 1 hit
PF00300, His_Phos_1, 1 hit

Protein Motif fingerprint database; a protein domain database

More...PRINTSi		PR00991, 6PFRUCTKNASE

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00855, PGAM, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF52540, SSF52540, 1 hit
SSF53254, SSF53254, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS00175, PG_MUTASE, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is in its mature form or if it represents the precursor.<p><a href='/help/sequence_processing' target='_top'>More...</a></p>Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O60825-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSGASSSEQN NNSYETKTPN LRMSEKKCSW ASYMTNSPTL IVMIGLPARG 
        60         70         80         90        100
KTYVSKKLTR YLNWIGVPTK VFNLGVYRRE AVKSYKSYDF FRHDNEEAMK 
       110        120        130        140        150
IRKQCALVAL EDVKAYLTEE NGQIAVFDAT NTTRERRDMI LNFAEQNSFK 
       160        170        180        190        200
VFFVESVCDD PDVIAANILE VKVSSPDYPE RNRENVMEDF LKRIECYKVT 
       210        220        230        240        250
YRPLDPDNYD KDLSFIKVIN VGQRFLVNRV QDYIQSKIVY YLMNIHVQPR 
       260        270        280        290        300
TIYLCRHGES EFNLLGKIGG DSGLSVRGKQ FAQALRKFLE EQEITDLKVW 
       310        320        330        340        350
TSQLKRTIQT AESLGVPYEQ WKILNEIDAG VCEEMTYAEI EKRYPEEFAL 
       360        370        380        390        400
RDQEKYLYRY PGGESYQDLV QRLEPVIMEL ERQGNVLVIS HQAVMRCLLA 
       410        420        430        440        450
YFLDKGADEL PYLRCPLHTI FKLTPVAYGC KVETIKLNVE AVNTHRDKPT 
       460        470        480        490        500
NNFPKNQTPV RMRRNSFTPL SSSNTIRRPR NYSVGSRPLK PLSPLRAQDM 

QEGAD
Length:505
Mass (Da):58,477
Last modified:January 24, 2001 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i5CD6A933A7EBF604
GO
Isoform 2 (identifier: O60825-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     451-505: NNFPKNQTPV...RAQDMQEGAD → AAETTLAVRRRPSAASLMLPC

Show »
Length:471
Mass (Da):54,406
Checksum:i2B9C8957889F4D61
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6RAZ1F6RAZ1_HUMAN
6-phosphofructo-2-kinase/fructose-2...
PFKFB2
125Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
F6XNB3F6XNB3_HUMAN
6-phosphofructo-2-kinase/fructose-2...
PFKFB2
96Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A087X2H4A0A087X2H4_HUMAN
6-phosphofructo-2-kinase/fructose-2...
PFKFB2
49Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
A0A087X278A0A087X278_HUMAN
6-phosphofructo-2-kinase/fructose-2...
PFKFB2
44Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti28Missing in CAA06605 (PubMed:9652401).Curated1
Sequence conflicti303 – 304QL → HV in CAA06606 (PubMed:9652401).Curated2
Sequence conflicti372R → L in CAA06606 (PubMed:9652401).Curated1
Sequence conflicti396R → H in CAA06606 (PubMed:9652401).Curated1
Sequence conflicti406G → D in CAA06606 (PubMed:9652401).Curated1
Sequence conflicti427A → T in CAA06606 (PubMed:9652401).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_004675451 – 505NNFPK…QEGAD → AAETTLAVRRRPSAASLMLP C in isoform 2. 3 PublicationsAdd BLAST55

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AJ005577 Genomic DNA Translation: CAA06605.1
AJ005578 mRNA Translation: CAA06606.1
AB044805 mRNA Translation: BAB19681.1
AF470623 mRNA Translation: AAL99386.1
AK292883 mRNA Translation: BAF85572.1
AL445493 Genomic DNA No translation available.
CH471100 Genomic DNA Translation: EAW93508.1
BC069350 mRNA Translation: AAH69350.1
BC069385 mRNA Translation: AAH69385.1
BC069583 mRNA Translation: AAH69583.1
BC069586 mRNA Translation: AAH69586.1
BC069600 mRNA Translation: AAH69600.1
BC075075 mRNA Translation: AAH75075.1
BC075076 mRNA Translation: AAH75076.1
BC112103 mRNA Translation: AAI12104.1
BC112105 mRNA Translation: AAI12106.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS31003.1 [O60825-2]
CCDS31004.1 [O60825-1]

NCBI Reference Sequences

More...RefSeqi		NP_001018063.1, NM_001018053.1 [O60825-2]
NP_006203.2, NM_006212.2 [O60825-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000367079; ENSP00000356046; ENSG00000123836 [O60825-2]
ENST00000367080; ENSP00000356047; ENSG00000123836 [O60825-1]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		5208

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:5208

UCSC genome browser

More...UCSCi		uc001hfg.4, human [O60825-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005577 Genomic DNA Translation: CAA06605.1
AJ005578 mRNA Translation: CAA06606.1
AB044805 mRNA Translation: BAB19681.1
AF470623 mRNA Translation: AAL99386.1
AK292883 mRNA Translation: BAF85572.1
AL445493 Genomic DNA No translation available.
CH471100 Genomic DNA Translation: EAW93508.1
BC069350 mRNA Translation: AAH69350.1
BC069385 mRNA Translation: AAH69385.1
BC069583 mRNA Translation: AAH69583.1
BC069586 mRNA Translation: AAH69586.1
BC069600 mRNA Translation: AAH69600.1
BC075075 mRNA Translation: AAH75075.1
BC075076 mRNA Translation: AAH75076.1
BC112103 mRNA Translation: AAI12104.1
BC112105 mRNA Translation: AAI12106.1
CCDSiCCDS31003.1 [O60825-2]
CCDS31004.1 [O60825-1]
RefSeqiNP_001018063.1, NM_001018053.1 [O60825-2]
NP_006203.2, NM_006212.2 [O60825-1]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5HTKX-ray2.01A/B1-505[»]
SMRiO60825
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi111229, 31 interactors
ComplexPortaliCPX-1994, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 complex
IntActiO60825, 25 interactors
MINTiO60825
STRINGi9606.ENSP00000356047

Chemistry databases

BindingDBiO60825
ChEMBLiCHEMBL3421525

PTM databases

DEPODiPFKFB2
iPTMnetiO60825
PhosphoSitePlusiO60825

Polymorphism and mutation databases

BioMutaiPFKFB2

Proteomic databases

EPDiO60825
jPOSTiO60825
MassIVEiO60825
MaxQBiO60825
PaxDbiO60825
PeptideAtlasiO60825
PRIDEiO60825
ProteomicsDBi49608 [O60825-1]
49609 [O60825-2]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		34592, 416 antibodies

Genome annotation databases

EnsembliENST00000367079; ENSP00000356046; ENSG00000123836 [O60825-2]
ENST00000367080; ENSP00000356047; ENSG00000123836 [O60825-1]
GeneIDi5208
KEGGihsa:5208
UCSCiuc001hfg.4, human [O60825-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		5208
DisGeNETi5208
EuPathDBiHostDB:ENSG00000123836.14

GeneCards: human genes, protein and diseases

More...GeneCardsi		PFKFB2
HGNCiHGNC:8873, PFKFB2
HPAiENSG00000123836, Low tissue specificity
MIMi171835, gene
neXtProtiNX_O60825
OpenTargetsiENSG00000123836
PharmGKBiPA33212

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG0234, Eukaryota
GeneTreeiENSGT00950000182835
HOGENOMiCLU_006383_1_1_1
InParanoidiO60825
KOiK19029
OMAiHDSCEWL
OrthoDBi392001at2759
PhylomeDBiO60825
TreeFamiTF313541

Enzyme and pathway databases

BioCyciMetaCyc:HS04690-MONOMER
BRENDAi2.7.1.105, 2681
3.1.3.46, 2681
PathwayCommonsiO60825
ReactomeiR-HSA-9634600, Regulation of glycolysis by fructose 2,6-bisphosphate metabolism
SABIO-RKiO60825
SIGNORiO60825

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		5208, 4 hits in 868 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		PFKFB2, human

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		PFKFB2

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		5208
PharosiO60825, Tchem

Protein Ontology

More...PROi		PR:O60825
RNActiO60825, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000123836, Expressed in islet of Langerhans and 213 other tissues
ExpressionAtlasiO60825, baseline and differential
GenevisibleiO60825, HS

Family and domain databases

CDDicd07067, HP_PGM_like, 1 hit
Gene3Di3.40.50.1240, 1 hit
InterProiView protein in InterPro
IPR003094, 6Pfruct_kin
IPR013079, 6Phosfructo_kin
IPR013078, His_Pase_superF_clade-1
IPR029033, His_PPase_superfam
IPR027417, P-loop_NTPase
IPR001345, PG/BPGM_mutase_AS
PANTHERiPTHR10606, PTHR10606, 1 hit
PfamiView protein in Pfam
PF01591, 6PF2K, 1 hit
PF00300, His_Phos_1, 1 hit
PRINTSiPR00991, 6PFRUCTKNASE
SMARTiView protein in SMART
SM00855, PGAM, 1 hit
SUPFAMiSSF52540, SSF52540, 1 hit
SSF53254, SSF53254, 1 hit
PROSITEiView protein in PROSITE
PS00175, PG_MUTASE, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiF262_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O60825
Secondary accession number(s): O60824
, Q5VVQ3, Q5VVQ4, Q9H3P1
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: January 24, 2001
Last modified: August 12, 2020
This is version 195 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
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