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Protein

General vesicular transport factor p115

Gene

USO1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.By similarity

GO - Molecular functioni

  • cadherin binding Source: BHF-UCL
  • protein transporter activity Source: GO_Central
  • RNA binding Source: UniProtKB

GO - Biological processi

Keywordsi

Biological processER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-HSA-162658 Golgi Cisternae Pericentriolar Stack Reorganization
R-HSA-204005 COPII-mediated vesicle transport
R-HSA-6807878 COPI-mediated anterograde transport
SIGNORiO60763

Names & Taxonomyi

Protein namesi
Recommended name:
General vesicular transport factor p115
Alternative name(s):
Protein USO1 homolog
Transcytosis-associated protein
Short name:
TAP
Vesicle-docking protein
Gene namesi
Name:USO1
Synonyms:VDP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 4

Organism-specific databases

EuPathDBiHostDB:ENSG00000138768.14
HGNCiHGNC:30904 USO1
MIMi603344 gene
neXtProtiNX_O60763

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi942S → A: Loss of phosphorylation. Promotes association with Golgi membranes. 1 Publication1
Mutagenesisi942S → D: Decreased association with Golgi membranes. 1 Publication1

Organism-specific databases

DisGeNETi8615
OpenTargetsiENSG00000138768
PharmGKBiPA162408713

Polymorphism and mutation databases

BioMutaiUSO1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000657741 – 962General vesicular transport factor p115Add BLAST962

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei50PhosphoserineCombined sources1
Modified residuei202N6-acetyllysineCombined sources1
Modified residuei942PhosphoserineCombined sources1 Publication1
Modified residuei952PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated in a cell cycle-specific manner; phosphorylated in interphase but not in mitotic cells. Dephosphorylated protein associates with the Golgi membrane; phosphorylation promotes dissociation.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO60763
MaxQBiO60763
PeptideAtlasiO60763
PRIDEiO60763
ProteomicsDBi49590
49591 [O60763-2]

PTM databases

iPTMnetiO60763
PhosphoSitePlusiO60763
SwissPalmiO60763

Expressioni

Gene expression databases

BgeeiENSG00000138768 Expressed in 240 organ(s), highest expression level in quadriceps femoris
CleanExiHS_USO1
ExpressionAtlasiO60763 baseline and differential
GenevisibleiO60763 HS

Organism-specific databases

HPAiCAB010108
HPA038282
HPA038283

Interactioni

Subunit structurei

Homodimer. Dimerizes by parallel association of the tails, resulting in an elongated structure with two globular head domains side by side, and a long rod-like tail structure (Probable). Interacts with MIF.Curated2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114173, 75 interactors
IntActiO60763, 29 interactors
MINTiO60763

Structurei

Secondary structure

1962
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO60763
SMRiO60763
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60763

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati20 – 60ARM 1Add BLAST41
Repeati61 – 121ARM 2Add BLAST61
Repeati123 – 163ARM 3Add BLAST41
Repeati166 – 207ARM 4Add BLAST42
Repeati208 – 253ARM 5Add BLAST46
Repeati255 – 310ARM 6Add BLAST56
Repeati311 – 354ARM 7Add BLAST44
Repeati363 – 408ARM 8Add BLAST46
Repeati420 – 459ARM 9Add BLAST40
Repeati473 – 513ARM 10Add BLAST41
Repeati518 – 571ARM 11Add BLAST54
Repeati573 – 630ARM 12Add BLAST58

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 637Globular headAdd BLAST637

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili638 – 930Sequence analysisAdd BLAST293

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi935 – 962Asp/Glu-rich (acidic)Add BLAST28

Domaini

Composed of a globular head, an elongated tail (coiled-coil) and a highly acidic C-terminal domain.1 Publication

Sequence similaritiesi

Belongs to the VDP/USO1/EDE1 family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

GeneTreeiENSGT00390000017018
HOGENOMiHOG000016409
HOVERGENiHBG018067
InParanoidiO60763
OMAiQQFFKEG
OrthoDBiEOG091G046H
PhylomeDBiO60763

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR000225 Armadillo
IPR006955 Uso1_p115_C
IPR006953 Vesicle_Uso1_P115_head
PfamiView protein in Pfam
PF04871 Uso1_p115_C, 1 hit
PF04869 Uso1_p115_head, 1 hit
SMARTiView protein in SMART
SM00185 ARM, 3 hits
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50176 ARM_REPEAT, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: O60763-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNFLRGVMGG QSAGPQHTEA ETIQKLCDRV ASSTLLDDRR NAVRALKSLS
60 70 80 90 100
KKYRLEVGIQ AMEHLIHVLQ TDRSDSEIIG YALDTLYNII SNEEEEEVEE
110 120 130 140 150
NSTRQSEDLG SQFTEIFIKQ QENVTLLLSL LEEFDFHVRW PGVKLLTSLL
160 170 180 190 200
KQLGPQVQQI ILVSPMGVSR LMDLLADSRE VIRNDGVLLL QALTRSNGAI
210 220 230 240 250
QKIVAFENAF ERLLDIISEE GNSDGGIVVE DCLILLQNLL KNNNSNQNFF
260 270 280 290 300
KEGSYIQRMK PWFEVGDENS GWSAQKVTNL HLMLQLVRVL VSPTNPPGAT
310 320 330 340 350
SSCQKAMFQC GLLQQLCTIL MATGVPADIL TETINTVSEV IRGCQVNQDY
360 370 380 390 400
FASVNAPSNP PRPAIVVLLM SMVNERQPFV LRCAVLYCFQ CFLYKNQKGQ
410 420 430 440 450
GEIVSTLLPS TIDATGNSVS AGQLLCGGLF STDSLSNWCA AVALAHALQE
460 470 480 490 500
NATQKEQLLR VQLATSIGNP PVSLLQQCTN ILSQGSKIQT RVGLLMLLCT
510 520 530 540 550
WLSNCPIAVT HFLHNSANVP FLTGQIAENL GEEEQLVQGL CALLLGISIY
560 570 580 590 600
FNDNSLESYM KEKLKQLIEK RIGKENFIEK LGFISKHELY SRASQKPQPN
610 620 630 640 650
FPSPEYMIFD HEFTKLVKEL EGVITKAIYK SSEEDKKEEE VKKTLEQHDN
660 670 680 690 700
IVTHYKNMIR EQDLQLEELR QQVSTLKCQN EQLQTAVTQQ VSQIQQHKDQ
710 720 730 740 750
YNLLKIQLGK DNQHQGSYSE GAQMNGIQPE EIGRLREEIE ELKRNQELLQ
760 770 780 790 800
SQLTEKDSMI ENMKSSQTSG TNEQSSAIVS ARDSEQVAEL KQELATLKSQ
810 820 830 840 850
LNSQSVEITK LQTEKQELLQ KTEAFAKSVE VQGETETIIA TKTTDVEGRL
860 870 880 890 900
SALLQETKEL KNEIKALSEE RTAIKEQLDS SNSTIAILQT EKDKLELEIT
910 920 930 940 950
DSKKEQDDLL VLLADQDQKI LSLKNKLKDL GHPVEEEDEL ESGDQEDEDD
960
ESEDPGKDLD HI
Length:962
Mass (Da):107,895
Last modified:March 6, 2007 - v2
Checksum:iC963652209031008
GO
Isoform 2 (identifier: O60763-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     98-98: V → VDDVE
     484-484: Q → QGDKIDRR

Show »
Length:973
Mass (Da):109,195
Checksum:i4DE9E8C5ED44514E
GO

Sequence cautioni

The sequence AAH06398 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti75D → G in CAD89917 (PubMed:17974005).Curated1
Sequence conflicti85T → I in BAA25300 (PubMed:9478999).Curated1
Sequence conflicti93Missing in AAH32654 (PubMed:15489334).Curated1
Sequence conflicti248N → Y in CAD89917 (PubMed:17974005).Curated1
Sequence conflicti650N → D in CAD89917 (PubMed:17974005).Curated1
Sequence conflicti877Q → R in BAG36947 (PubMed:14702039).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_03912098V → VDDVE in isoform 2. 1 Publication1
Alternative sequenceiVSP_039121484Q → QGDKIDRR in isoform 2. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86326 mRNA Translation: BAA25300.1
AK314289 mRNA Translation: BAG36947.1
AL832010 mRNA Translation: CAD89917.1
AC110615 Genomic DNA No translation available.
AC104828 Genomic DNA No translation available.
BC006398 mRNA Translation: AAH06398.1 Sequence problems.
BC032654 mRNA Translation: AAH32654.1
CCDSiCCDS75144.1 [O60763-1]
CCDS77929.1 [O60763-2]
RefSeqiNP_001276978.1, NM_001290049.1 [O60763-2]
NP_003706.2, NM_003715.3 [O60763-1]
UniGeneiHs.744877

Genome annotation databases

EnsembliENST00000264904; ENSP00000264904; ENSG00000138768 [O60763-2]
ENST00000514213; ENSP00000444850; ENSG00000138768 [O60763-1]
GeneIDi8615
KEGGihsa:8615
UCSCiuc003hiv.5 human [O60763-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86326 mRNA Translation: BAA25300.1
AK314289 mRNA Translation: BAG36947.1
AL832010 mRNA Translation: CAD89917.1
AC110615 Genomic DNA No translation available.
AC104828 Genomic DNA No translation available.
BC006398 mRNA Translation: AAH06398.1 Sequence problems.
BC032654 mRNA Translation: AAH32654.1
CCDSiCCDS75144.1 [O60763-1]
CCDS77929.1 [O60763-2]
RefSeqiNP_001276978.1, NM_001290049.1 [O60763-2]
NP_003706.2, NM_003715.3 [O60763-1]
UniGeneiHs.744877

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2W3CX-ray2.22A53-629[»]
ProteinModelPortaliO60763
SMRiO60763
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114173, 75 interactors
IntActiO60763, 29 interactors
MINTiO60763

PTM databases

iPTMnetiO60763
PhosphoSitePlusiO60763
SwissPalmiO60763

Polymorphism and mutation databases

BioMutaiUSO1

Proteomic databases

EPDiO60763
MaxQBiO60763
PeptideAtlasiO60763
PRIDEiO60763
ProteomicsDBi49590
49591 [O60763-2]

Protocols and materials databases

DNASUi8615
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000264904; ENSP00000264904; ENSG00000138768 [O60763-2]
ENST00000514213; ENSP00000444850; ENSG00000138768 [O60763-1]
GeneIDi8615
KEGGihsa:8615
UCSCiuc003hiv.5 human [O60763-1]

Organism-specific databases

CTDi8615
DisGeNETi8615
EuPathDBiHostDB:ENSG00000138768.14
GeneCardsiUSO1
HGNCiHGNC:30904 USO1
HPAiCAB010108
HPA038282
HPA038283
MIMi603344 gene
neXtProtiNX_O60763
OpenTargetsiENSG00000138768
PharmGKBiPA162408713
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00390000017018
HOGENOMiHOG000016409
HOVERGENiHBG018067
InParanoidiO60763
OMAiQQFFKEG
OrthoDBiEOG091G046H
PhylomeDBiO60763

Enzyme and pathway databases

ReactomeiR-HSA-162658 Golgi Cisternae Pericentriolar Stack Reorganization
R-HSA-204005 COPII-mediated vesicle transport
R-HSA-6807878 COPI-mediated anterograde transport
SIGNORiO60763

Miscellaneous databases

ChiTaRSiUSO1 human
EvolutionaryTraceiO60763
GeneWikiiUSO1
GenomeRNAii8615
PROiPR:O60763
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000138768 Expressed in 240 organ(s), highest expression level in quadriceps femoris
CleanExiHS_USO1
ExpressionAtlasiO60763 baseline and differential
GenevisibleiO60763 HS

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR000225 Armadillo
IPR006955 Uso1_p115_C
IPR006953 Vesicle_Uso1_P115_head
PfamiView protein in Pfam
PF04871 Uso1_p115_C, 1 hit
PF04869 Uso1_p115_head, 1 hit
SMARTiView protein in SMART
SM00185 ARM, 3 hits
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50176 ARM_REPEAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUSO1_HUMAN
AccessioniPrimary (citable) accession number: O60763
Secondary accession number(s): B2RAQ0
, Q6PK63, Q86TB8, Q8N592
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: March 6, 2007
Last modified: September 12, 2018
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 4
    Human chromosome 4: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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