Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sorting nexin-2

Gene

SNX2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) (PubMed:16179610). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex (PubMed:17101778). The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity (PubMed:23085988). Required for retrograde endosome-to-TGN transport of TGN38 (PubMed:20138391). Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN (PubMed:20604901).1 Publication5 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei183Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei185Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei211Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei235Phosphatidylinositol 3-phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-HSA-432722 Golgi Associated Vesicle Biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting nexin-2
Alternative name(s):
Transformation-related gene 9 protein
Short name:
TRG-9
Gene namesi
Name:SNX2
ORF Names:TRG9
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 5

Organism-specific databases

EuPathDBiHostDB:ENSG00000205302.6
HGNCiHGNC:11173 SNX2
MIMi605929 gene
neXtProtiNX_O60749

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi182 – 184RRF → AAA: Decreases KALRN-dependent lamellipodium formation; no effect on interaction with KALRN. 1 Publication3
Mutagenesisi211K → A: Abolishes phosphatidylinositol phosphate binding. Abolishes endosomal location. 1 Publication1
Mutagenesisi426K → A: Decreases KALRN-dependent lamellipodium formation; no effect on interaction with KALRN; when associated with A-428. 1 Publication1
Mutagenesisi428R → A: Decreases KALRN-dependent lamellipodium formation; no effect on interaction with KALRN; when associated with A-426. 1 Publication1

Organism-specific databases

DisGeNETi6643
OpenTargetsiENSG00000205302
PharmGKBiPA36012

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002138381 – 519Sorting nexin-2Add BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei97PhosphoserineBy similarity1
Modified residuei101PhosphothreonineCombined sources1
Modified residuei104PhosphothreonineCombined sources1
Modified residuei117PhosphoserineCombined sources1
Modified residuei119PhosphoserineCombined sources1
Modified residuei185PhosphoserineCombined sources1
Modified residuei277PhosphoserineCombined sources1
Modified residuei469N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO60749
MaxQBiO60749
PaxDbiO60749
PeptideAtlasiO60749
PRIDEiO60749
ProteomicsDBi49584

PTM databases

iPTMnetiO60749
PhosphoSitePlusiO60749
SwissPalmiO60749

Expressioni

Gene expression databases

BgeeiENSG00000205302 Expressed in 232 organ(s), highest expression level in intestine
CleanExiHS_SNX2
ExpressionAtlasiO60749 baseline and differential
GenevisibleiO60749 HS

Organism-specific databases

HPAiHPA037400

Interactioni

Subunit structurei

Predominantly forms heterodimers with BAR domain-containing sorting nexins SNX5, SNX6 and SNX32; can self-associate to form homodimers (PubMed:23085988). The heterodimers are proposed to self-assemble into helical arrays on the membrane to stabilize and expand local membrane curvature underlying endosomal tubule formation. Thought to be a component of the originally described retromer complex (also called SNX-BAR retromer) which is a pentamer containing the heterotrimeric retromer cargo-selective complex (CSC), also descibed as vacuolar protein sorting subcomplex (VPS), and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity (Probable). Interacts with SNX5, SNX6, SNX32, VPS26A, VPS29, VPS35, FNBP1, KALRN, RHOG (GDP-bound form) (PubMed:11438682, PubMed:14596906, PubMed:17101778, PubMed:19935774., PubMed:19619496, PubMed:20604901, PubMed:23085988).3 Publications7 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi112526, 90 interactors
CORUMiO60749
IntActiO60749, 33 interactors
MINTiO60749
STRINGi9606.ENSP00000368831

Structurei

3D structure databases

ProteinModelPortaliO60749
SMRiO60749
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini140 – 269PXPROSITE-ProRule annotationAdd BLAST130
Domaini299 – 519BARBy similarityAdd BLAST221

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni260 – 519Interaction with RhoG1 PublicationAdd BLAST260
Regioni278 – 295Membrane-binding amphipathic helix1 PublicationAdd BLAST18

Domaini

The BAR domain is able to sense membrane curvature upon dimerization. Membrane remodeling seems to implicate insertion of a N-terminal amphipatric helix (AH) in the membrane (Probable).2 Publications

Sequence similaritiesi

Belongs to the sorting nexin family.Curated

Phylogenomic databases

eggNOGiKOG2273 Eukaryota
COG5391 LUCA
GeneTreeiENSGT00910000144062
HOGENOMiHOG000293327
HOVERGENiHBG000618
InParanoidiO60749
KOiK17917
OMAiERYLMRT
OrthoDBiEOG091G06FB
PhylomeDBiO60749
TreeFamiTF313698

Family and domain databases

CDDicd07282 PX_SNX2, 1 hit
Gene3Di1.20.1270.60, 1 hit
3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR001683 Phox
IPR036871 PX_dom_sf
IPR028653 SNX2
IPR037918 SNX2_PX
IPR039358 Sorting_nexin-like
IPR005329 Sorting_nexin_N
IPR015404 Vps5_C
PANTHERiPTHR10555 PTHR10555, 1 hit
PTHR10555:SF31 PTHR10555:SF31, 1 hit
PfamiView protein in Pfam
PF00787 PX, 1 hit
PF03700 Sorting_nexin, 1 hit
PF09325 Vps5, 1 hit
SMARTiView protein in SMART
SM00312 PX, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50195 PX, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O60749-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAEREPPPL GDGKPTDFED LEDGEDLFTS TVSTLESSPS SPEPASLPAE
60 70 80 90 100
DISANSNGPK PTEVVLDDDR EDLFAEATEE VSLDSPEREP ILSSEPSPAV
110 120 130 140 150
TPVTPTTLIA PRIESKSMSA PVIFDRSREE IEEEANGDIF DIEIGVSDPE
160 170 180 190 200
KVGDGMNAYM AYRVTTKTSL SMFSKSEFSV KRRFSDFLGL HSKLASKYLH
210 220 230 240 250
VGYIVPPAPE KSIVGMTKVK VGKEDSSSTE FVEKRRAALE RYLQRTVKHP
260 270 280 290 300
TLLQDPDLRQ FLESSELPRA VNTQALSGAG ILRMVNKAAD AVNKMTIKMN
310 320 330 340 350
ESDAWFEEKQ QQFENLDQQL RKLHVSVEAL VCHRKELSAN TAAFAKSAAM
360 370 380 390 400
LGNSEDHTAL SRALSQLAEV EEKIDQLHQE QAFADFYMFS ELLSDYIRLI
410 420 430 440 450
AAVKGVFDHR MKCWQKWEDA QITLLKKREA EAKMMVANKP DKIQQAKNEI
460 470 480 490 500
REWEAKVQQG ERDFEQISKT IRKEVGRFEK ERVKDFKTVI IKYLESLVQT
510
QQQLIKYWEA FLPEAKAIA
Length:519
Mass (Da):58,471
Last modified:May 30, 2006 - v2
Checksum:i7DC07DFA523312B5
GO
Isoform 2 (identifier: O60749-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-117: Missing.

Show »
Length:402
Mass (Da):46,098
Checksum:i4CF80EFD3B9C160D
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D6RC15D6RC15_HUMAN
Sorting nexin-2
SNX2
265Annotation score:
D6RBT1D6RBT1_HUMAN
Sorting nexin-2
SNX2
76Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87E → A in AAQ02693 (Ref. 3) Curated1
Sequence conflicti146V → I in BAH12394 (PubMed:14702039).Curated1
Sequence conflicti331V → F (Ref. 1) Curated1
Sequence conflicti331V → F (PubMed:9819414).Curated1
Sequence conflicti384A → S (Ref. 1) Curated1
Sequence conflicti384A → S (PubMed:9819414).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0547851 – 117Missing in isoform 2. 1 PublicationAdd BLAST117

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF065482 mRNA Translation: AAC17181.1
AF043453 mRNA Translation: AAB99852.1
AY272044 mRNA Translation: AAQ02693.1
BT009841 mRNA Translation: AAP88843.1
AK023581 mRNA Translation: BAG51206.1
AK293671 mRNA Translation: BAG57115.1
AK296596 mRNA Translation: BAH12394.1
AC008669 Genomic DNA No translation available.
AC093267 Genomic DNA No translation available.
CH471086 Genomic DNA Translation: EAW48884.1
BC003382 mRNA Translation: AAH03382.1
CCDSiCCDS34217.1 [O60749-1]
CCDS64234.1 [O60749-2]
RefSeqiNP_001265128.1, NM_001278199.1 [O60749-2]
NP_003091.2, NM_003100.3 [O60749-1]
UniGeneiHs.125352
Hs.713554

Genome annotation databases

EnsembliENST00000379516; ENSP00000368831; ENSG00000205302 [O60749-1]
ENST00000514949; ENSP00000421663; ENSG00000205302 [O60749-2]
GeneIDi6643
KEGGihsa:6643
UCSCiuc003kte.5 human [O60749-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF065482 mRNA Translation: AAC17181.1
AF043453 mRNA Translation: AAB99852.1
AY272044 mRNA Translation: AAQ02693.1
BT009841 mRNA Translation: AAP88843.1
AK023581 mRNA Translation: BAG51206.1
AK293671 mRNA Translation: BAG57115.1
AK296596 mRNA Translation: BAH12394.1
AC008669 Genomic DNA No translation available.
AC093267 Genomic DNA No translation available.
CH471086 Genomic DNA Translation: EAW48884.1
BC003382 mRNA Translation: AAH03382.1
CCDSiCCDS34217.1 [O60749-1]
CCDS64234.1 [O60749-2]
RefSeqiNP_001265128.1, NM_001278199.1 [O60749-2]
NP_003091.2, NM_003100.3 [O60749-1]
UniGeneiHs.125352
Hs.713554

3D structure databases

ProteinModelPortaliO60749
SMRiO60749
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112526, 90 interactors
CORUMiO60749
IntActiO60749, 33 interactors
MINTiO60749
STRINGi9606.ENSP00000368831

PTM databases

iPTMnetiO60749
PhosphoSitePlusiO60749
SwissPalmiO60749

Proteomic databases

EPDiO60749
MaxQBiO60749
PaxDbiO60749
PeptideAtlasiO60749
PRIDEiO60749
ProteomicsDBi49584

Protocols and materials databases

DNASUi6643
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379516; ENSP00000368831; ENSG00000205302 [O60749-1]
ENST00000514949; ENSP00000421663; ENSG00000205302 [O60749-2]
GeneIDi6643
KEGGihsa:6643
UCSCiuc003kte.5 human [O60749-1]

Organism-specific databases

CTDi6643
DisGeNETi6643
EuPathDBiHostDB:ENSG00000205302.6
GeneCardsiSNX2
HGNCiHGNC:11173 SNX2
HPAiHPA037400
MIMi605929 gene
neXtProtiNX_O60749
OpenTargetsiENSG00000205302
PharmGKBiPA36012
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2273 Eukaryota
COG5391 LUCA
GeneTreeiENSGT00910000144062
HOGENOMiHOG000293327
HOVERGENiHBG000618
InParanoidiO60749
KOiK17917
OMAiERYLMRT
OrthoDBiEOG091G06FB
PhylomeDBiO60749
TreeFamiTF313698

Enzyme and pathway databases

ReactomeiR-HSA-432722 Golgi Associated Vesicle Biogenesis

Miscellaneous databases

ChiTaRSiSNX2 human
GeneWikiiSNX2
GenomeRNAii6643
PROiPR:O60749
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000205302 Expressed in 232 organ(s), highest expression level in intestine
CleanExiHS_SNX2
ExpressionAtlasiO60749 baseline and differential
GenevisibleiO60749 HS

Family and domain databases

CDDicd07282 PX_SNX2, 1 hit
Gene3Di1.20.1270.60, 1 hit
3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR001683 Phox
IPR036871 PX_dom_sf
IPR028653 SNX2
IPR037918 SNX2_PX
IPR039358 Sorting_nexin-like
IPR005329 Sorting_nexin_N
IPR015404 Vps5_C
PANTHERiPTHR10555 PTHR10555, 1 hit
PTHR10555:SF31 PTHR10555:SF31, 1 hit
PfamiView protein in Pfam
PF00787 PX, 1 hit
PF03700 Sorting_nexin, 1 hit
PF09325 Vps5, 1 hit
SMARTiView protein in SMART
SM00312 PX, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50195 PX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSNX2_HUMAN
AccessioniPrimary (citable) accession number: O60749
Secondary accession number(s): B3KN44
, B4DEK4, B7Z408, O43650, P82862, Q53XK8, Q597H6, Q9BTS8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 30, 2006
Last modified: September 12, 2018
This is version 168 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. SIMILARITY comments
    Index of protein domains and families
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again