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Protein

Leupaxin

Gene

LPXN

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Transcriptional coactivator for androgen receptor (AR) and serum response factor (SRF). Contributes to the regulation of cell adhesion, spreading and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events. Suppresses the integrin-induced tyrosine phosphorylation of paxillin (PXN). May play a critical role as an adapter protein in the formation of the adhesion zone in osteoclasts. Negatively regulates B-cell antigen receptor (BCR) signaling.4 Publications

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • transcription coregulator activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processCell adhesion, Transcription, Transcription regulation
LigandMetal-binding, Zinc

Enzyme and pathway databases

SignaLinkiO60711

Names & Taxonomyi

Protein namesi
Recommended name:
Leupaxin
Gene namesi
Name:LPXN
Synonyms:LDLP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000110031.12
HGNCiHGNC:14061 LPXN
MIMi605390 gene
neXtProtiNX_O60711

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9404
OpenTargetsiENSG00000110031
PharmGKBiPA30441

Polymorphism and mutation databases

BioMutaiLPXN

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000758361 – 386LeupaxinAdd BLAST386

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineCombined sources1
Modified residuei19PhosphoserineCombined sources1
Modified residuei22Phosphotyrosine1 Publication1
Modified residuei54PhosphoserineBy similarity1
Modified residuei62Phosphotyrosine1 Publication1
Modified residuei72Phosphotyrosine; by LYN2 Publications1
Modified residuei81PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated on tyrosine residues. Phosphorylation on Tyr-72 is important for its inhibitory function. Bombesin stimulates phosphorylation on Tyr-22, Tyr-62 and Tyr-72.2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO60711
MaxQBiO60711
PaxDbiO60711
PeptideAtlasiO60711
PRIDEiO60711
ProteomicsDBi49535
49536 [O60711-2]

PTM databases

iPTMnetiO60711
PhosphoSitePlusiO60711

Expressioni

Tissue specificityi

Macrophages, monocytes and osteoclasts (at protein level). Strongly expressed in cells and tissues of hematopoietic origin. Highest expression in lymphoid tissues such as spleen, lymph node, thymus and appendix and in the vascular smooth muscle. Lower levels in bone marrow and fetal liver. Also expressed in peripheral blood lymphocytes and a number of hematopoietic cell lines. Very low levels found in epithelial cell lines. Expressed in prostate cancer (PCa) cells and its expression intensity is directly linked to PCa progression.3 Publications

Gene expression databases

BgeeiENSG00000110031 Expressed in 184 organ(s), highest expression level in mononuclear cell
CleanExiHS_LPXN
ExpressionAtlasiO60711 baseline and differential
GenevisibleiO60711 HS

Organism-specific databases

HPAiCAB046478
HPA043741
HPA061441

Interactioni

Subunit structurei

Interacts with PTPN22 (By similarity). Interacts with unphosphorylated ITGA4. Interacts with PTK2B/PYK2, PTPN12, AR and SRF. Interacts (via LD motif 3) with LYN and the interaction is induced upon B-cell antigen receptor (BCR) activation. Interacts (via LD motif 3) with PTK2/FAK.By similarity6 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi114801, 55 interactors
IntActiO60711, 36 interactors
MINTiO60711
STRINGi9606.ENSP00000431284

Structurei

Secondary structure

1386
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO60711
SMRiO60711
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60711

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini150 – 208LIM zinc-binding 1PROSITE-ProRule annotationAdd BLAST59
Domaini209 – 267LIM zinc-binding 2PROSITE-ProRule annotationAdd BLAST59
Domaini268 – 326LIM zinc-binding 3PROSITE-ProRule annotationAdd BLAST59
Domaini327 – 386LIM zinc-binding 4PROSITE-ProRule annotationAdd BLAST60

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi3 – 15LD motif 1Add BLAST13
Motifi70 – 82LD motif 2Add BLAST13
Motifi92 – 103LD motif 3Add BLAST12

Domaini

The LIM domain 3 is critical for focal adhesion targeting and the suppression of paxillin (PXN) tyrosine phosphorylation. The LIM domain 3 alone or both LIM domains 3 and 4 can mediate interaction with AR.

Sequence similaritiesi

Belongs to the paxillin family.Curated

Keywords - Domaini

LIM domain, Repeat

Phylogenomic databases

eggNOGiKOG1703 Eukaryota
ENOG410XRD4 LUCA
GeneTreeiENSGT00760000118910
HOGENOMiHOG000018764
HOVERGENiHBG001512
InParanoidiO60711
OMAiELMAHLC
OrthoDBiEOG091G05AW
PhylomeDBiO60711
TreeFamiTF314113

Family and domain databases

InterProiView protein in InterPro
IPR034961 Leupaxin
IPR017305 Tgfb1i1/Leupaxin/TGFB1I1
IPR001781 Znf_LIM
PANTHERiPTHR24216:SF23 PTHR24216:SF23, 1 hit
PfamiView protein in Pfam
PF00412 LIM, 4 hits
PIRSFiPIRSF037881 Leupaxin, 1 hit
SMARTiView protein in SMART
SM00132 LIM, 4 hits
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 4 hits
PS50023 LIM_DOMAIN_2, 4 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O60711-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEELDALLEE LERSTLQDSD EYSNPAPLPL DQHSRKETNL DETSEILSIQ
60 70 80 90 100
DNTSPLPAQL VYTTNIQELN VYSEAQEPKE SPPPSKTSAA AQLDELMAHL
110 120 130 140 150
TEMQAKVAVR ADAGKKHLPD KQDHKASLDS MLGGLEQELQ DLGIATVPKG
160 170 180 190 200
HCASCQKPIA GKVIHALGQS WHPEHFVCTH CKEEIGSSPF FERSGLAYCP
210 220 230 240 250
NDYHQLFSPR CAYCAAPILD KVLTAMNQTW HPEHFFCSHC GEVFGAEGFH
260 270 280 290 300
EKDKKPYCRK DFLAMFSPKC GGCNRPVLEN YLSAMDTVWH PECFVCGDCF
310 320 330 340 350
TSFSTGSFFE LDGRPFCELH YHHRRGTLCH GCGQPITGRC ISAMGYKFHP
360 370 380
EHFVCAFCLT QLSKGIFREQ NDKTYCQPCF NKLFPL
Length:386
Mass (Da):43,332
Last modified:August 1, 1998 - v1
Checksum:iC8D2BE61FAB11F3A
GO
Isoform 2 (identifier: O60711-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: MEEL → MSTLLISSS

Note: No experimental confirmation available.
Show »
Length:391
Mass (Da):43,750
Checksum:iA8F1FFD11D4DB7BD
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B7Z5P7B7Z5P7_HUMAN
cDNA FLJ51550, highly similar to Le...
LPXN
366Annotation score:
E9PNX9E9PNX9_HUMAN
Leupaxin
LPXN
106Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2E → D in CAG38768 (Ref. 3) Curated1
Sequence conflicti14S → T in BAD96885 (Ref. 4) Curated1
Sequence conflicti100L → M in CAG38768 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_050152148P → T. Corresponds to variant dbSNP:rs12271558Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0426551 – 4MEEL → MSTLLISSS in isoform 2. 1 Publication4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062075 mRNA Translation: AAC16014.1
AK300955 mRNA Translation: BAG62583.1
AK313306 mRNA Translation: BAG36111.1
CR536531 mRNA Translation: CAG38768.1
AK223165 mRNA Translation: BAD96885.1
AP001350 Genomic DNA No translation available.
AP003557 Genomic DNA No translation available.
CH471076 Genomic DNA Translation: EAW73808.1
BC019035 mRNA Translation: AAH19035.1
CCDSiCCDS53635.1 [O60711-2]
CCDS7969.1 [O60711-1]
RefSeqiNP_001137467.1, NM_001143995.2 [O60711-2]
NP_001294880.1, NM_001307951.1
NP_004802.1, NM_004811.2 [O60711-1]
UniGeneiHs.125474

Genome annotation databases

EnsembliENST00000395074; ENSP00000378512; ENSG00000110031 [O60711-1]
ENST00000528954; ENSP00000431284; ENSG00000110031 [O60711-2]
GeneIDi9404
KEGGihsa:9404
UCSCiuc001nmw.4 human [O60711-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062075 mRNA Translation: AAC16014.1
AK300955 mRNA Translation: BAG62583.1
AK313306 mRNA Translation: BAG36111.1
CR536531 mRNA Translation: CAG38768.1
AK223165 mRNA Translation: BAD96885.1
AP001350 Genomic DNA No translation available.
AP003557 Genomic DNA No translation available.
CH471076 Genomic DNA Translation: EAW73808.1
BC019035 mRNA Translation: AAH19035.1
CCDSiCCDS53635.1 [O60711-2]
CCDS7969.1 [O60711-1]
RefSeqiNP_001137467.1, NM_001143995.2 [O60711-2]
NP_001294880.1, NM_001307951.1
NP_004802.1, NM_004811.2 [O60711-1]
UniGeneiHs.125474

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1X3HNMR-A260-326[»]
4XEFX-ray2.50B/C/E/F1-20[»]
4XEKX-ray1.79C86-104[»]
4XEVX-ray2.01C/F86-104[»]
ProteinModelPortaliO60711
SMRiO60711
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114801, 55 interactors
IntActiO60711, 36 interactors
MINTiO60711
STRINGi9606.ENSP00000431284

PTM databases

iPTMnetiO60711
PhosphoSitePlusiO60711

Polymorphism and mutation databases

BioMutaiLPXN

Proteomic databases

EPDiO60711
MaxQBiO60711
PaxDbiO60711
PeptideAtlasiO60711
PRIDEiO60711
ProteomicsDBi49535
49536 [O60711-2]

Protocols and materials databases

DNASUi9404
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000395074; ENSP00000378512; ENSG00000110031 [O60711-1]
ENST00000528954; ENSP00000431284; ENSG00000110031 [O60711-2]
GeneIDi9404
KEGGihsa:9404
UCSCiuc001nmw.4 human [O60711-1]

Organism-specific databases

CTDi9404
DisGeNETi9404
EuPathDBiHostDB:ENSG00000110031.12
GeneCardsiLPXN
HGNCiHGNC:14061 LPXN
HPAiCAB046478
HPA043741
HPA061441
MIMi605390 gene
neXtProtiNX_O60711
OpenTargetsiENSG00000110031
PharmGKBiPA30441
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1703 Eukaryota
ENOG410XRD4 LUCA
GeneTreeiENSGT00760000118910
HOGENOMiHOG000018764
HOVERGENiHBG001512
InParanoidiO60711
OMAiELMAHLC
OrthoDBiEOG091G05AW
PhylomeDBiO60711
TreeFamiTF314113

Enzyme and pathway databases

SignaLinkiO60711

Miscellaneous databases

ChiTaRSiLPXN human
EvolutionaryTraceiO60711
GeneWikiiLPXN
GenomeRNAii9404
PROiPR:O60711
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000110031 Expressed in 184 organ(s), highest expression level in mononuclear cell
CleanExiHS_LPXN
ExpressionAtlasiO60711 baseline and differential
GenevisibleiO60711 HS

Family and domain databases

InterProiView protein in InterPro
IPR034961 Leupaxin
IPR017305 Tgfb1i1/Leupaxin/TGFB1I1
IPR001781 Znf_LIM
PANTHERiPTHR24216:SF23 PTHR24216:SF23, 1 hit
PfamiView protein in Pfam
PF00412 LIM, 4 hits
PIRSFiPIRSF037881 Leupaxin, 1 hit
SMARTiView protein in SMART
SM00132 LIM, 4 hits
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 4 hits
PS50023 LIM_DOMAIN_2, 4 hits
ProtoNetiSearch...

Entry informationi

Entry nameiLPXN_HUMAN
AccessioniPrimary (citable) accession number: O60711
Secondary accession number(s): B2R8B4
, B4DV71, Q53FW6, Q6FI07
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 7, 2018
This is version 168 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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