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Protein

Protein-tyrosine sulfotransferase 2

Gene

TPST2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.1 Publication

Miscellaneous

Substrate peptides must be flexible in order to adopt an L-shaped conformation in the deep binding cleft.

Catalytic activityi

3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei99Proton donor/acceptor1 Publication1
Sitei158Transition state stabilizer1 Publication1
Binding sitei183PAPSCombined sources1 Publication1
Binding sitei191PAPSCombined sources1 Publication1
Binding sitei195PAPSCombined sources1 Publication1
Binding sitei238PAPSCombined sources1 Publication1
Sitei285Transition state stabilizer1 Publication1
Binding sitei300PAPSCombined sources1 Publication1

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • protein-tyrosine sulfotransferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BRENDAi2.8.2.20 2681
ReactomeiR-HSA-156584 Cytosolic sulfonation of small molecules

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-tyrosine sulfotransferase 2 (EC:2.8.2.201 Publication)
Alternative name(s):
Tyrosylprotein sulfotransferase 2
Short name:
TPST-2
Gene namesi
Name:TPST2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 22

Organism-specific databases

EuPathDBiHostDB:ENSG00000128294.15
HGNCiHGNC:12021 TPST2
MIMi603126 gene
neXtProtiNX_O60704

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini26 – 377LumenalSequence analysisAdd BLAST352

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi78R → A: Strongly reduced enzymatic activity. 1 Publication1
Mutagenesisi99E → A: Loss of sulfotransferase activity. 1 Publication1
Mutagenesisi101R → A: Prevents dimerization and strongly decreases enzyme activity. 1 Publication1
Mutagenesisi113W → A: Prevents dimerization and decreases enzyme activity. 1 Publication1
Mutagenesisi158K → A: Nearly complete loss of enzymatic activity. 1 Publication1
Mutagenesisi198T → A: Slightly decreased sulfotransferase activity. 1 Publication1
Mutagenesisi285S → A: Abolishes sulfotransferase activity. 1 Publication1

Organism-specific databases

DisGeNETi8459
OpenTargetsiENSG00000128294
PharmGKBiPA36700

Chemistry databases

ChEMBLiCHEMBL3178

Polymorphism and mutation databases

BioMutaiTPST2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001898291 – 377Protein-tyrosine sulfotransferase 2Add BLAST377

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi96 ↔ 156Combined sources1 Publication
Disulfide bondi225 ↔ 233Combined sources1 Publication
Glycosylationi343N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi368N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiO60704
MaxQBiO60704
PaxDbiO60704
PeptideAtlasiO60704
PRIDEiO60704
ProteomicsDBi49532

PTM databases

iPTMnetiO60704
PhosphoSitePlusiO60704

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Gene expression databases

BgeeiENSG00000128294 Expressed in 205 organ(s), highest expression level in body of pancreas
CleanExiHS_TPST2
ExpressionAtlasiO60704 baseline and differential
GenevisibleiO60704 HS

Organism-specific databases

HPAiHPA021054

Interactioni

Subunit structurei

Homodimer (PubMed:23481380). Can also form heterodimers with TPST1 (PubMed:25660941).2 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114037, 54 interactors
STRINGi9606.ENSP00000339813

Chemistry databases

BindingDBiO60704

Structurei

Secondary structure

1377
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO60704
SMRiO60704
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60704

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni78 – 82PAPS bindingCombined sources5
Regioni101 – 105Interaction with peptide substrate1 Publication5
Regioni285 – 294PAPS bindingCombined sources10

Sequence similaritiesi

Belongs to the protein sulfotransferase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3988 Eukaryota
ENOG410XQ2X LUCA
GeneTreeiENSGT00390000006030
HOGENOMiHOG000253020
HOVERGENiHBG001270
InParanoidiO60704
KOiK01021
OMAiGKDKCLP
OrthoDBiEOG091G09YJ
PhylomeDBiO60704
TreeFamiTF312910

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR026634 TPST
PANTHERiPTHR12788 PTHR12788, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 6 potential isoforms that are computationally mapped.Show allAlign All

O60704-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MRLSVRRVLL AAGCALVLVL AVQLGQQVLE CRAVLAGLRS PRGAMRPEQE
60 70 80 90 100
ELVMVGTNHV EYRYGKAMPL IFVGGVPRSG TTLMRAMLDA HPEVRCGEET
110 120 130 140 150
RIIPRVLAMR QAWSKSGREK LRLDEAGVTD EVLDAAMQAF ILEVIAKHGE
160 170 180 190 200
PARVLCNKDP FTLKSSVYLS RLFPNSKFLL MVRDGRASVH SMITRKVTIA
210 220 230 240 250
GFDLSSYRDC LTKWNKAIEV MYAQCMEVGK EKCLPVYYEQ LVLHPRRSLK
260 270 280 290 300
LILDFLGIAW SDAVLHHEDL IGKPGGVSLS KIERSTDQVI KPVNLEALSK
310 320 330 340 350
WTGHIPGDVV RDMAQIAPML AQLGYDPYAN PPNYGNPDPF VINNTQRVLK
360 370
GDYKTPANLK GYFQVNQNST SSHLGSS
Length:377
Mass (Da):41,912
Last modified:August 1, 1998 - v1
Checksum:iA658E50151FDBC12
GO

Computationally mapped potential isoform sequencesi

There are 6 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
B1AHJ7B1AHJ7_HUMAN
Protein-tyrosine sulfotransferase
TPST2
120Annotation score:
B1AHJ8B1AHJ8_HUMAN
Protein-tyrosine sulfotransferase
TPST2
64Annotation score:
B1AHJ6B1AHJ6_HUMAN
Protein-tyrosine sulfotransferase
TPST2
141Annotation score:
B1AHJ5B1AHJ5_HUMAN
Protein-tyrosine sulfotransferase
TPST2
168Annotation score:
B1AHK0B1AHK0_HUMAN
Protein-tyrosine sulfotransferase
TPST2
47Annotation score:
B1AHJ9B1AHJ9_HUMAN
Protein-tyrosine sulfotransferase
TPST2
48Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26Q → L in CAB66558 (PubMed:11230166).Curated1
Sequence conflicti26Q → L in CAG38559 (Ref. 6) Curated1
Sequence conflicti73V → E in CAB66558 (PubMed:11230166).Curated1
Sequence conflicti73V → E in CAG38559 (Ref. 6) Curated1
Sequence conflicti115K → M in CAG38559 (Ref. 6) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061254 mRNA Translation: AAC34296.1
AF049891 mRNA Translation: AAC36061.1
AJ006198 mRNA Translation: CAA06906.1
AL136623 mRNA Translation: CAB66558.1
CR456597 mRNA Translation: CAG30483.1
CR533528 mRNA Translation: CAG38559.1
AK074538 mRNA Translation: BAG51969.1
AK075139 mRNA Translation: BAG52071.1
Z95115 Genomic DNA No translation available.
CH471095 Genomic DNA Translation: EAW59726.1
BC001057 mRNA Translation: AAH01057.1
BC017509 mRNA Translation: AAH17509.1
CCDSiCCDS13839.1
RefSeqiNP_001008566.1, NM_001008566.1
NP_003586.3, NM_003595.3
UniGeneiHs.632768

Genome annotation databases

EnsembliENST00000338754; ENSP00000339813; ENSG00000128294
ENST00000398110; ENSP00000381180; ENSG00000128294
ENST00000403880; ENSP00000385192; ENSG00000128294
GeneIDi8459
KEGGihsa:8459
UCSCiuc003acw.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061254 mRNA Translation: AAC34296.1
AF049891 mRNA Translation: AAC36061.1
AJ006198 mRNA Translation: CAA06906.1
AL136623 mRNA Translation: CAB66558.1
CR456597 mRNA Translation: CAG30483.1
CR533528 mRNA Translation: CAG38559.1
AK074538 mRNA Translation: BAG51969.1
AK075139 mRNA Translation: BAG52071.1
Z95115 Genomic DNA No translation available.
CH471095 Genomic DNA Translation: EAW59726.1
BC001057 mRNA Translation: AAH01057.1
BC017509 mRNA Translation: AAH17509.1
CCDSiCCDS13839.1
RefSeqiNP_001008566.1, NM_001008566.1
NP_003586.3, NM_003595.3
UniGeneiHs.632768

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3AP1X-ray1.90A/B43-359[»]
3AP2X-ray2.40A/B43-359[»]
3AP3X-ray3.50A/B/C/D43-377[»]
ProteinModelPortaliO60704
SMRiO60704
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114037, 54 interactors
STRINGi9606.ENSP00000339813

Chemistry databases

BindingDBiO60704
ChEMBLiCHEMBL3178

PTM databases

iPTMnetiO60704
PhosphoSitePlusiO60704

Polymorphism and mutation databases

BioMutaiTPST2

Proteomic databases

EPDiO60704
MaxQBiO60704
PaxDbiO60704
PeptideAtlasiO60704
PRIDEiO60704
ProteomicsDBi49532

Protocols and materials databases

DNASUi8459
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000338754; ENSP00000339813; ENSG00000128294
ENST00000398110; ENSP00000381180; ENSG00000128294
ENST00000403880; ENSP00000385192; ENSG00000128294
GeneIDi8459
KEGGihsa:8459
UCSCiuc003acw.4 human

Organism-specific databases

CTDi8459
DisGeNETi8459
EuPathDBiHostDB:ENSG00000128294.15
GeneCardsiTPST2
HGNCiHGNC:12021 TPST2
HPAiHPA021054
MIMi603126 gene
neXtProtiNX_O60704
OpenTargetsiENSG00000128294
PharmGKBiPA36700
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3988 Eukaryota
ENOG410XQ2X LUCA
GeneTreeiENSGT00390000006030
HOGENOMiHOG000253020
HOVERGENiHBG001270
InParanoidiO60704
KOiK01021
OMAiGKDKCLP
OrthoDBiEOG091G09YJ
PhylomeDBiO60704
TreeFamiTF312910

Enzyme and pathway databases

BRENDAi2.8.2.20 2681
ReactomeiR-HSA-156584 Cytosolic sulfonation of small molecules

Miscellaneous databases

ChiTaRSiTPST2 human
EvolutionaryTraceiO60704
GenomeRNAii8459
PROiPR:O60704
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000128294 Expressed in 205 organ(s), highest expression level in body of pancreas
CleanExiHS_TPST2
ExpressionAtlasiO60704 baseline and differential
GenevisibleiO60704 HS

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR026634 TPST
PANTHERiPTHR12788 PTHR12788, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTPST2_HUMAN
AccessioniPrimary (citable) accession number: O60704
Secondary accession number(s): B3KQA7, Q6FI98, Q9H0V4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 7, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 22
    Human chromosome 22: entries, gene names and cross-references to MIM
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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