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Protein

Protein arginine N-methyltransferase 3

Gene

PRMT3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.

Activity regulationi

Inhibited by N-ethylmaleimide and high concentrations of zinc chloride.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei230S-adenosyl-L-methionineBy similarity1
Binding sitei239S-adenosyl-L-methionine1
Binding sitei263S-adenosyl-L-methionine; via carbonyl oxygen1
Binding sitei285S-adenosyl-L-methionine1
Binding sitei314S-adenosyl-L-methionine1
Active sitei329By similarity1
Active sitei338By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri48 – 71C2H2-typeAdd BLAST24

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
LigandMetal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-3214858 RMTs methylate histone arginines
R-HSA-8876725 Protein methylation
SABIO-RKiO60678

Names & Taxonomyi

Protein namesi
Recommended name:
Protein arginine N-methyltransferase 3 (EC:2.1.1.-)
Alternative name(s):
Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3
Gene namesi
Name:PRMT3
Synonyms:HRMT1L3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000185238.12
HGNCiHGNC:30163 PRMT3
MIMi603190 gene
neXtProtiNX_O60678

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi87Y → C: Markedly reduced affinity for RPS2. 1 Publication1
Mutagenesisi87Y → E: Markedly reduced affinity for RPS2. 1 Publication1
Mutagenesisi87Y → F: No effect on interaction with RPS2. 1 Publication1

Organism-specific databases

DisGeNETi10196
OpenTargetsiENSG00000185238
PharmGKBiPA29462

Chemistry databases

ChEMBLiCHEMBL5891
DrugBankiDB01752 S-Adenosyl-L-Homocysteine
GuidetoPHARMACOLOGYi1254

Polymorphism and mutation databases

BioMutaiPRMT3

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002123262 – 531Protein arginine N-methyltransferase 3Add BLAST530

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylcysteineCombined sources1
Modified residuei25PhosphoserineCombined sources1 Publication1
Modified residuei27PhosphoserineCombined sources1 Publication1
Modified residuei171PhosphoserineCombined sources1

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei87Not phosphorylated1 Publication1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO60678
MaxQBiO60678
PaxDbiO60678
PeptideAtlasiO60678
PRIDEiO60678
ProteomicsDBi49522
49523 [O60678-2]

PTM databases

iPTMnetiO60678
PhosphoSitePlusiO60678

Expressioni

Gene expression databases

BgeeiENSG00000185238 Expressed in 209 organ(s), highest expression level in corpus callosum
CleanExiHS_PRMT3
ExpressionAtlasiO60678 baseline and differential
GenevisibleiO60678 HS

Organism-specific databases

HPAiCAB022083
HPA007832

Interactioni

Subunit structurei

Monomer or homodimer (By similarity). Interacts with EPB41L3; this inhibits methylation of target proteins. Interacts with the 40S ribosomal protein RPS2.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SCN5AQ145242EBI-2809009,EBI-726858

Protein-protein interaction databases

BioGridi115491, 48 interactors
IntActiO60678, 16 interactors
MINTiO60678
STRINGi9606.ENSP00000331879

Structurei

Secondary structure

1531
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO60678
SMRiO60678
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60678

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini217 – 531SAM-dependent MTase PRMT-typePROSITE-ProRule annotationAdd BLAST315

Domaini

The zinc-finger is responsible for substrate specificity.By similarity

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri48 – 71C2H2-typeAdd BLAST24

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1499 Eukaryota
ENOG410XQYH LUCA
GeneTreeiENSGT00550000074406
HOGENOMiHOG000198521
HOVERGENiHBG001793
InParanoidiO60678
KOiK11436
OrthoDBiEOG091G0ADC
PhylomeDBiO60678
TreeFamiTF323587

Family and domain databases

InterProiView protein in InterPro
IPR025799 Arg_MeTrfase
IPR029063 SAM-dependent_MTases
IPR036236 Znf_C2H2_sf
IPR013087 Znf_C2H2_type
SUPFAMiSSF53335 SSF53335, 1 hit
SSF57667 SSF57667, 1 hit
PROSITEiView protein in PROSITE
PS51678 SAM_MT_PRMT, 1 hit
PS00028 ZINC_FINGER_C2H2_1, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.iShow all

Isoform 1 (identifier: O60678-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MCSLASGATG GRGAVENEED LPELSDSGDE AAWEDEDDAD LPHGKQQTPC
60 70 80 90 100
LFCNRLFTSA EETFSHCKSE HQFNIDSMVH KHGLEFYGYI KLINFIRLKN
110 120 130 140 150
PTVEYMNSIY NPVPWEKEEY LKPVLEDDLL LQFDVEDLYE PVSVPFSYPN
160 170 180 190 200
GLSENTSVVE KLKHMEARAL SAEAALARAR EDLQKMKQFA QDFVMHTDVR
210 220 230 240 250
TCSSSTSVIA DLQEDEDGVY FSSYGHYGIH EEMLKDKIRT ESYRDFIYQN
260 270 280 290 300
PHIFKDKVVL DVGCGTGILS MFAAKAGAKK VLGVDQSEIL YQAMDIIRLN
310 320 330 340 350
KLEDTITLIK GKIEEVHLPV EKVDVIISEW MGYFLLFESM LDSVLYAKNK
360 370 380 390 400
YLAKGGSVYP DICTISLVAV SDVNKHADRI AFWDDVYGFK MSCMKKAVIP
410 420 430 440 450
EAVVEVLDPK TLISEPCGIK HIDCHTTSIS DLEFSSDFTL KITRTSMCTA
460 470 480 490 500
IAGYFDIYFE KNCHNRVVFS TGPQSTKTHW KQTVFLLEKP FSVKAGEALK
510 520 530
GKVTVHKNKK DPRSLTVTLT LNNSTQTYGL Q
Length:531
Mass (Da):59,903
Last modified:March 28, 2018 - v4
Checksum:i71EC59E2BEDBE7AC
GO
Isoform 2 (identifier: O60678-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     11-99: GRGAVENEED...IKLINFIRLK → YSHLLKKHFHTVSLSISLILTAWFINM

Show »
Length:469
Mass (Da):52,902
Checksum:iEBAAAC40A78A7B14
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PLF6E9PLF6_HUMAN
Protein arginine N-methyltransferas...
PRMT3
46Annotation score:
E9PRB4E9PRB4_HUMAN
Protein arginine N-methyltransferas...
PRMT3
54Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21L → E in AAC39837 (PubMed:9642256).Curated1
Sequence conflicti103V → F in BAG62289 (PubMed:14702039).Curated1
Sequence conflicti510K → R in BAG62289 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_024584440L → V. Corresponds to variant dbSNP:rs3758805Ensembl.1
Natural variantiVAR_030943470S → C. Corresponds to variant dbSNP:rs11025585Ensembl.1
Natural variantiVAR_024585508N → S. Corresponds to variant dbSNP:rs6483700Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_04033011 – 99GRGAV…FIRLK → YSHLLKKHFHTVSLSISLIL TAWFINM in isoform 2. 1 PublicationAdd BLAST89

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK300591 mRNA Translation: BAG62289.1
AC025972 Genomic DNA No translation available.
AC108005 Genomic DNA No translation available.
KC877394 Genomic DNA No translation available.
KC877396 Genomic DNA No translation available.
KF459543 Genomic DNA No translation available.
BC037544 mRNA Translation: AAH37544.1
BC064831 mRNA Translation: AAH64831.1
AF059531 mRNA Translation: AAC39837.1
CCDSiCCDS44554.1 [O60678-2]
CCDS7853.1 [O60678-1]
RefSeqiNP_005779.1, NM_005788.3 [O60678-1]
UniGeneiHs.152337

Genome annotation databases

EnsembliENST00000331079; ENSP00000331879; ENSG00000185238 [O60678-1]
ENST00000437750; ENSP00000397766; ENSG00000185238 [O60678-2]
GeneIDi10196
KEGGihsa:10196
UCSCiuc001mqb.4 human [O60678-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiANM3_HUMAN
AccessioniPrimary (citable) accession number: O60678
Secondary accession number(s): A0A0A0MSN7, B4DUC7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: March 28, 2018
Last modified: September 12, 2018
This is version 166 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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