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Protein

Cyclin-T1

Gene

CCNT1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II).2 Publications
(Microbial infection) In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.2 Publications

Miscellaneous

Interaction between Tat and cyclin-T1 requires zinc.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei261Essential for interacting with HIV-1 Tat1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCyclin
Biological processCell cycle, Cell division, Host-virus interaction, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-176034 Interactions of Tat with host cellular proteins
R-HSA-2173796 SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-9018519 Estrogen-dependent gene expression
SignaLinkiO60563
SIGNORiO60563

Names & Taxonomyi

Protein namesi
Recommended name:
Cyclin-T1
Short name:
CycT1
Short name:
Cyclin-T
Gene namesi
Name:CCNT1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiHostDB:ENSG00000129315.9
HGNCiHGNC:1599 CCNT1
MIMi143055 gene
neXtProtiNX_O60563

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi261C → Y: Loss of HIV-1 Tat transactivation. 1 Publication1

Organism-specific databases

DisGeNETi904
OpenTargetsiENSG00000129315
PharmGKBiPA26163

Chemistry databases

ChEMBLiCHEMBL2108

Polymorphism and mutation databases

BioMutaiCCNT1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000804911 – 726Cyclin-T1Add BLAST726

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei117PhosphoserineCombined sources1
Modified residuei340PhosphoserineCombined sources1
Cross-linki342Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei388PhosphoserineCombined sources1
Modified residuei390N6-acetyllysineCombined sources1
Cross-linki415Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki481Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei495PhosphoserineCombined sources1
Modified residuei499PhosphoserineCombined sources1
Modified residuei564PhosphoserineCombined sources1
Modified residuei577PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO60563
MaxQBiO60563
PaxDbiO60563
PeptideAtlasiO60563
PRIDEiO60563
ProteomicsDBi49468

PTM databases

iPTMnetiO60563
PhosphoSitePlusiO60563

Expressioni

Tissue specificityi

Ubiquitously expressed.

Gene expression databases

BgeeiENSG00000129315 Expressed in 221 organ(s), highest expression level in female reproductive system
CleanExiHS_CCNT1
ExpressionAtlasiO60563 baseline and differential
GenevisibleiO60563 HS

Organism-specific databases

HPAiCAB009016
HPA004892

Interactioni

Subunit structurei

Cyclin-T1 is the predominant cyclin that associates with CDK9 to form a heterodimer called P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin (PubMed:10393184). Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs (PubMed:17643375). Interacts with BRD4, probably to target chromatin binding (PubMed:16109376, PubMed:16109377). Interacts with MDFIC (PubMed:12944466). Interacts with HSF1 (PubMed:27189267). Interacts with HTATSF1 (PubMed:10913173). Interacts with AFF4 (PubMed:12065898). Interacts with TBX21 (PubMed:27292648).9 Publications
(Microbial infection) Interacts with the transactivation region of HIV-1, HIV-2 and SIV Tat.5 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi107343, 82 interactors
ComplexPortaliCPX-222 Positive transcription elongation factor B, CDK9-cyclinT1 complex
CORUMiO60563
DIPiDIP-29891N
IntActiO60563, 32 interactors
MINTiO60563
STRINGi9606.ENSP00000261900

Chemistry databases

BindingDBiO60563

Structurei

Secondary structure

1726
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO60563
SMRiO60563
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO60563

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili384 – 425Sequence analysisAdd BLAST42

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi253 – 270Nuclear localization signal, and interaction with Tat-TAR RNASequence analysisAdd BLAST18

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi506 – 530His-richAdd BLAST25
Compositional biasi548 – 629Ser-richAdd BLAST82
Compositional biasi708 – 725Pro-richAdd BLAST18

Sequence similaritiesi

Belongs to the cyclin family. Cyclin C subfamily.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0834 Eukaryota
COG5333 LUCA
GeneTreeiENSGT00760000119191
HOGENOMiHOG000013208
HOVERGENiHBG050843
InParanoidiO60563
KOiK15188
OMAiKRIWNWR
OrthoDBiEOG091G03Z2
PhylomeDBiO60563
TreeFamiTF101014

Family and domain databases

CDDicd00043 CYCLIN, 1 hit
InterProiView protein in InterPro
IPR028863 CCNT1
IPR013763 Cyclin-like
IPR036915 Cyclin-like_sf
IPR006671 Cyclin_N
PANTHERiPTHR10026:SF42 PTHR10026:SF42, 1 hit
PfamiView protein in Pfam
PF00134 Cyclin_N, 1 hit
SMARTiView protein in SMART
SM00385 CYCLIN, 1 hit
SUPFAMiSSF47954 SSF47954, 2 hits

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O60563-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEGERKNNNK RWYFTREQLE NSPSRRFGVD PDKELSYRQQ AANLLQDMGQ
60 70 80 90 100
RLNVSQLTIN TAIVYMHRFY MIQSFTQFPG NSVAPAALFL AAKVEEQPKK
110 120 130 140 150
LEHVIKVAHT CLHPQESLPD TRSEAYLQQV QDLVILESII LQTLGFELTI
160 170 180 190 200
DHPHTHVVKC TQLVRASKDL AQTSYFMATN SLHLTTFSLQ YTPPVVACVC
210 220 230 240 250
IHLACKWSNW EIPVSTDGKH WWEYVDATVT LELLDELTHE FLQILEKTPN
260 270 280 290 300
RLKRIWNWRA CEAAKKTKAD DRGTDEKTSE QTILNMISQS SSDTTIAGLM
310 320 330 340 350
SMSTSTTSAV PSLPVSEESS SNLTSVEMLP GKRWLSSQPS FKLEPTQGHR
360 370 380 390 400
TSENLALTGV DHSLPQDGSN AFISQKQNSK SVPSAKVSLK EYRAKHAEEL
410 420 430 440 450
AAQKRQLENM EANVKSQYAY AAQNLLSHHD SHSSVILKMP IEGSENPERP
460 470 480 490 500
FLEKADKTAL KMRIPVAGGD KAASSKPEEI KMRIKVHAAA DKHNSVEDSV
510 520 530 540 550
TKSREHKEKH KTHPSNHHHH HNHHSHKHSH SQLPVGTGNK RPGDPKHSSQ
560 570 580 590 600
TSNLAHKTYS LSSSFSSSSS TRKRGPSEET GGAVFDHPAK IAKSTKSSSL
610 620 630 640 650
NFSFPSLPTM GQMPGHSSDT SGLSFSQPSC KTRVPHSKLD KGPTGANGHN
660 670 680 690 700
TTQTIDYQDT VNMLHSLLSA QGVQPTQPTA FEFVRPYSDY LNPRSGGISS
710 720
RSGNTDKPRP PPLPSEPPPP LPPLPK
Length:726
Mass (Da):80,685
Last modified:August 1, 1998 - v1
Checksum:i4637EFB2DDEDFE13
GO
Isoform 2 (identifier: O60563-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     181-184: SLHL → RTDT
     185-726: Missing.

Show »
Length:184
Mass (Da):21,206
Checksum:i331262690A9FCE9B
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1W2PQ16A0A1W2PQ16_HUMAN
Cyclin-T1
CCNT1
184Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77Q → R in AAC39664 (PubMed:9499409).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_053054362H → R. Corresponds to variant dbSNP:rs17123261Ensembl.1
Natural variantiVAR_069400541R → C1 PublicationCorresponds to variant dbSNP:rs201951577Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_054569181 – 184SLHL → RTDT in isoform 2. 1 Publication4
Alternative sequenceiVSP_054570185 – 726Missing in isoform 2. 1 PublicationAdd BLAST542

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045161 mRNA Translation: AAC39638.1
AF048730 mRNA Translation: AAC39664.1
EF688064 mRNA Translation: ABV58572.1
AC079951 Genomic DNA No translation available.
CCDSiCCDS61109.1 [O60563-2]
CCDS8766.1 [O60563-1]
RefSeqiNP_001231.2, NM_001240.3 [O60563-1]
NP_001264771.1, NM_001277842.1 [O60563-2]
UniGeneiHs.92308

Genome annotation databases

EnsembliENST00000261900; ENSP00000261900; ENSG00000129315 [O60563-1]
ENST00000417344; ENSP00000399845; ENSG00000129315 [O60563-2]
ENST00000618666; ENSP00000481035; ENSG00000129315 [O60563-2]
GeneIDi904
KEGGihsa:904
UCSCiuc001rsd.5 human [O60563-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045161 mRNA Translation: AAC39638.1
AF048730 mRNA Translation: AAC39664.1
EF688064 mRNA Translation: ABV58572.1
AC079951 Genomic DNA No translation available.
CCDSiCCDS61109.1 [O60563-2]
CCDS8766.1 [O60563-1]
RefSeqiNP_001231.2, NM_001240.3 [O60563-1]
NP_001264771.1, NM_001277842.1 [O60563-2]
UniGeneiHs.92308

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2PK2X-ray2.67A/B/C/D1-281[»]
3BLHX-ray2.48B2-259[»]
3BLQX-ray2.90B2-259[»]
3BLRX-ray2.80B2-259[»]
3LQ5X-ray3.00B2-259[»]
3MI9X-ray2.10B1-266[»]
3MIAX-ray3.00B1-266[»]
3MY1X-ray2.80B2-259[»]
3TN8X-ray2.95B2-259[»]
3TNHX-ray3.20B1-259[»]
3TNIX-ray3.23B1-259[»]
4BCFX-ray3.01B2-259[»]
4BCGX-ray3.08B2-259[»]
4BCHX-ray2.96B2-259[»]
4BCIX-ray3.10B2-259[»]
4BCJX-ray3.16B2-259[»]
4EC8X-ray3.60B2-259[»]
4EC9X-ray3.21B2-259[»]
4IMYX-ray2.94B/D/F1-264[»]
4OGRX-ray3.00B/F/K1-264[»]
4OR5X-ray2.90B/G1-266[»]
5L1ZX-ray5.90B1-264[»]
6GZHX-ray3.17B1-726[»]
ProteinModelPortaliO60563
SMRiO60563
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107343, 82 interactors
ComplexPortaliCPX-222 Positive transcription elongation factor B, CDK9-cyclinT1 complex
CORUMiO60563
DIPiDIP-29891N
IntActiO60563, 32 interactors
MINTiO60563
STRINGi9606.ENSP00000261900

Chemistry databases

BindingDBiO60563
ChEMBLiCHEMBL2108

PTM databases

iPTMnetiO60563
PhosphoSitePlusiO60563

Polymorphism and mutation databases

BioMutaiCCNT1

Proteomic databases

EPDiO60563
MaxQBiO60563
PaxDbiO60563
PeptideAtlasiO60563
PRIDEiO60563
ProteomicsDBi49468

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261900; ENSP00000261900; ENSG00000129315 [O60563-1]
ENST00000417344; ENSP00000399845; ENSG00000129315 [O60563-2]
ENST00000618666; ENSP00000481035; ENSG00000129315 [O60563-2]
GeneIDi904
KEGGihsa:904
UCSCiuc001rsd.5 human [O60563-1]

Organism-specific databases

CTDi904
DisGeNETi904
EuPathDBiHostDB:ENSG00000129315.9
GeneCardsiCCNT1
HGNCiHGNC:1599 CCNT1
HPAiCAB009016
HPA004892
MIMi143055 gene
neXtProtiNX_O60563
OpenTargetsiENSG00000129315
PharmGKBiPA26163
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0834 Eukaryota
COG5333 LUCA
GeneTreeiENSGT00760000119191
HOGENOMiHOG000013208
HOVERGENiHBG050843
InParanoidiO60563
KOiK15188
OMAiKRIWNWR
OrthoDBiEOG091G03Z2
PhylomeDBiO60563
TreeFamiTF101014

Enzyme and pathway databases

ReactomeiR-HSA-112382 Formation of RNA Pol II elongation complex
R-HSA-167152 Formation of HIV elongation complex in the absence of HIV Tat
R-HSA-167200 Formation of HIV-1 elongation complex containing HIV-1 Tat
R-HSA-167238 Pausing and recovery of Tat-mediated HIV elongation
R-HSA-167243 Tat-mediated HIV elongation arrest and recovery
R-HSA-167246 Tat-mediated elongation of the HIV-1 transcript
R-HSA-167287 HIV elongation arrest and recovery
R-HSA-167290 Pausing and recovery of HIV elongation
R-HSA-176034 Interactions of Tat with host cellular proteins
R-HSA-2173796 SMAD2/SMAD3:SMAD4 heterotrimer regulates transcription
R-HSA-674695 RNA Polymerase II Pre-transcription Events
R-HSA-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-HSA-6807505 RNA polymerase II transcribes snRNA genes
R-HSA-75955 RNA Polymerase II Transcription Elongation
R-HSA-9018519 Estrogen-dependent gene expression
SignaLinkiO60563
SIGNORiO60563

Miscellaneous databases

ChiTaRSiCCNT1 human
EvolutionaryTraceiO60563
GeneWikiiCyclin_T1
GenomeRNAii904
PROiPR:O60563
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000129315 Expressed in 221 organ(s), highest expression level in female reproductive system
CleanExiHS_CCNT1
ExpressionAtlasiO60563 baseline and differential
GenevisibleiO60563 HS

Family and domain databases

CDDicd00043 CYCLIN, 1 hit
InterProiView protein in InterPro
IPR028863 CCNT1
IPR013763 Cyclin-like
IPR036915 Cyclin-like_sf
IPR006671 Cyclin_N
PANTHERiPTHR10026:SF42 PTHR10026:SF42, 1 hit
PfamiView protein in Pfam
PF00134 Cyclin_N, 1 hit
SMARTiView protein in SMART
SM00385 CYCLIN, 1 hit
SUPFAMiSSF47954 SSF47954, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiCCNT1_HUMAN
AccessioniPrimary (citable) accession number: O60563
Secondary accession number(s): A9XU13, E7EX76, O60581
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: October 10, 2018
This is version 190 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  4. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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