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Protein

Nuclear export mediator factor NEMF

Gene

NEMF

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. NEMF is responsible for selective recognition of stalled 60S subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety. NEMF is important for the stable association of LTN1 to the complex (PubMed:25578875). May indirectly play a role in nuclear export (PubMed:16103875).2 Publications

Miscellaneous

Active in normal lung tissue, but is inactive in several lung cancer cell lines.

GO - Biological processi

  • nuclear export Source: UniProtKB

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear export mediator factor NEMF
Alternative name(s):
Antigen NY-CO-1
Serologically defined colon cancer antigen 1
Gene namesi
Name:NEMF
Synonyms:SDCCAG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 14

Organism-specific databases

EuPathDBiHostDB:ENSG00000165525.17
HGNCiHGNC:10663 NEMF
MIMi608378 gene
neXtProtiNX_O60524

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi9147
OpenTargetsiENSG00000165525
PharmGKBiPA35593

Polymorphism and mutation databases

BioMutaiNEMF

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000976421 – 1076Nuclear export mediator factor NEMFAdd BLAST1076

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei7PhosphothreonineCombined sources1
Modified residuei417PhosphoserineCombined sources1
Modified residuei747PhosphoserineCombined sources1
Modified residuei748PhosphoserineCombined sources1
Modified residuei763PhosphoserineCombined sources1
Modified residuei831PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO60524
MaxQBiO60524
PaxDbiO60524
PeptideAtlasiO60524
PRIDEiO60524
ProteomicsDBi49458
49459 [O60524-2]
49460 [O60524-3]
49461 [O60524-4]
49462 [O60524-5]

PTM databases

iPTMnetiO60524
PhosphoSitePlusiO60524

Expressioni

Tissue specificityi

Expressed in brain, heart, liver, lung, spleen, and skeletal muscle. Also expressed at lower levels in stomach and testis.1 Publication

Gene expression databases

BgeeiENSG00000165525 Expressed in 242 organ(s), highest expression level in frontal cortex
CleanExiHS_SDCCAG1
ExpressionAtlasiO60524 baseline and differential
GenevisibleiO60524 HS

Organism-specific databases

HPAiHPA004160

Interactioni

Subunit structurei

Component of the ribosome quality control complex (RQC), composed of at least the E3 ubiquitin ligase LTN1 and NEMF (PubMed:25578875). The complex probably also contains TCF25 as well as VCP/p97 and its ubiquitin-binding cofactors (By similarity). RQC forms a stable complex with 60S ribosomal subunits (PubMed:25578875). Interacts (via its N-terminus) with XPO1 (By similarity).By similarity1 Publication

Protein-protein interaction databases

IntActiO60524, 10 interactors
MINTiO60524
STRINGi9606.ENSP00000298310

Structurei

3D structure databases

ProteinModelPortaliO60524
SMRiO60524
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili296 – 359Sequence analysisAdd BLAST64
Coiled coili483 – 514Sequence analysisAdd BLAST32
Coiled coili869 – 894Sequence analysisAdd BLAST26

Domaini

The N-terminal domain contains a nuclear export signal. The C-terminal domain may interact with cargo proteins (By similarity).By similarity

Sequence similaritiesi

Belongs to the NEMF family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG2030 Eukaryota
COG1293 LUCA
GeneTreeiENSGT00390000018516
HOVERGENiHBG079170
InParanoidiO60524
OMAiFEWPKNV
OrthoDBiEOG091G02MR
PhylomeDBiO60524
TreeFamiTF300515

Family and domain databases

InterProiView protein in InterPro
IPR021846 DUF3441
IPR008532 DUF814
PfamiView protein in Pfam
PF11923 DUF3441, 1 hit
PF05670 DUF814, 1 hit

Sequences (5+)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 5 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O60524-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MKSRFSTIDL RAVLAELNAS LLGMRVNNVY DVDNKTYLIR LQKPDFKATL
60 70 80 90 100
LLESGIRIHT TEFEWPKNMM PSSFAMKCRK HLKSRRLVSA KQLGVDRIVD
110 120 130 140 150
FQFGSDEAAY HLIIELYDRG NIVLTDYEYV ILNILRFRTD EADDVKFAVR
160 170 180 190 200
ERYPLDHARA AEPLLTLERL TEIVASAPKG ELLKRVLNPL LPYGPALIEH
210 220 230 240 250
CLLENGFSGN VKVDEKLETK DIEKVLVSLQ KAEDYMKTTS NFSGKGYIIQ
260 270 280 290 300
KREIKPSLEA DKPVEDILTY EEFHPFLFSQ HSQCPYIEFE SFDKAVDEFY
310 320 330 340 350
SKIEGQKIDL KALQQEKQAL KKLDNVRKDH ENRLEALQQA QEIDKLKGEL
360 370 380 390 400
IEMNLQIVDR AIQVVRSALA NQIDWTEIGL IVKEAQAQGD PVASAIKELK
410 420 430 440 450
LQTNHVTMLL RNPYLLSEEE DDDVDGDVNV EKNETEPPKG KKKKQKNKQL
460 470 480 490 500
QKPQKNKPLL VDVDLSLSAY ANAKKYYDHK RYAAKKTQKT VEAAEKAFKS
510 520 530 540 550
AEKKTKQTLK EVQTVTSIQK ARKVYWFEKF LWFISSENYL IIGGRDQQQN
560 570 580 590 600
EIIVKRYLTP GDIYVHADLH GATSCVIKNP TGEPIPPRTL TEAGTMALCY
610 620 630 640 650
SAAWDARVIT SAWWVYHHQV SKTAPTGEYL TTGSFMIRGK KNFLPPSYLM
660 670 680 690 700
MGFSFLFKVD ESCVWRHQGE RKVRVQDEDM ETLASCTSEL ISEEMEQLDG
710 720 730 740 750
GDTSSDEDKE EHETPVEVEL MTQVDQEDIT LQSGRDELNE ELIQEESSED
760 770 780 790 800
EGEYEEVRKD QDSVGEMKDE GEETLNYPDT TIDLSHLQPQ RSIQKLASKE
810 820 830 840 850
ESSNSSDSKS QSRRHLSAKE RREMKKKKLP SDSGDLEALE GKDKEKESTV
860 870 880 890 900
HIETHQNTSK NVAAVQPMKR GQKSKMKKMK EKYKDQDEED RELIMKLLGS
910 920 930 940 950
AGSNKEEKGK KGKKGKTKDE PVKKQPQKPR GGQRVSDNIK KETPFLEVIT
960 970 980 990 1000
HELQDFAVDD PHDDKEEQDL DQQGNEENLF DSLTGQPHPE DVLLFAIPIC
1010 1020 1030 1040 1050
APYTTMTNYK YKVKLTPGVQ KKGKAAKTAL NSFMHSKEAT AREKDLFRSV
1060 1070
KDTDLSRNIP GKVKVSAPNL LNVKRK
Length:1,076
Mass (Da):122,954
Last modified:November 24, 2009 - v4
Checksum:iFCC5F95A0DB22F79
GO
Isoform 2 (identifier: O60524-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-21: MKSRFSTIDLRAVLAELNASL → MPKTCQCYVGTKTTNPSAWPS
     22-821: Missing.

Show »
Length:276
Mass (Da):31,151
Checksum:i49B37757681B0E51
GO
Isoform 3 (identifier: O60524-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     562-582: Missing.

Show »
Length:1,055
Mass (Da):120,761
Checksum:i6D69BA826390F146
GO
Isoform 4 (identifier: O60524-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     78-119: Missing.

Show »
Length:1,034
Mass (Da):118,025
Checksum:i8B42E666C724AB60
GO
Isoform 5 (identifier: O60524-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     221-245: Missing.

Note: No experimental confirmation available.
Show »
Length:1,051
Mass (Da):120,140
Checksum:iF0CA1E8446FC3739
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3V5V3G3V5V3_HUMAN
Nuclear export mediator factor NEMF
NEMF
998Annotation score:
G3V547G3V547_HUMAN
Nuclear export mediator factor NEMF
NEMF
121Annotation score:
G3V4D9G3V4D9_HUMAN
Nuclear export mediator factor NEMF
NEMF
178Annotation score:

Sequence cautioni

The sequence AAC18036 differs from that shown. Reason: Frameshift at position 1065.Curated
The sequence AAH06001 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH20794 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH56687 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAI07765 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence BAG52763 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAG58070 differs from that shown. Intron retention.Curated
The sequence BAG58070 differs from that shown. Reason: Frameshift at positions 246 and 1025.Curated
The sequence BAG58070 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti53E → K in BAG56774 (PubMed:14702039).Curated1
Sequence conflicti438P → S in AAH56687 (PubMed:15489334).Curated1
Sequence conflicti495E → G in BAG56774 (PubMed:14702039).Curated1
Sequence conflicti560P → PV in BAG58070 (PubMed:14702039).Curated1
Sequence conflicti593A → V in CAE45889 (PubMed:17974005).Curated1
Sequence conflicti696E → K in BAG65465 (PubMed:14702039).Curated1
Sequence conflicti766E → V in BX648753 (PubMed:17974005).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_034488257S → C2 PublicationsCorresponds to variant dbSNP:rs3100906Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0083961 – 21MKSRF…LNASL → MPKTCQCYVGTKTTNPSAWP S in isoform 2. 1 PublicationAdd BLAST21
Alternative sequenceiVSP_01046222 – 821Missing in isoform 2. 1 PublicationAdd BLAST800
Alternative sequenceiVSP_04106478 – 119Missing in isoform 4. 2 PublicationsAdd BLAST42
Alternative sequenceiVSP_041065221 – 245Missing in isoform 5. 1 PublicationAdd BLAST25
Alternative sequenceiVSP_041066562 – 582Missing in isoform 3. 1 PublicationAdd BLAST21

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000913 mRNA No translation available.
AK093783 mRNA Translation: BAG52763.1 Different initiation.
AK293236 mRNA Translation: BAG56774.1
AK295010 mRNA Translation: BAG58070.1 Sequence problems.
AK304695 mRNA Translation: BAG65465.1
BX640804 mRNA Translation: CAE45886.1
BX640807 mRNA Translation: CAE45889.1
BX648753 mRNA No translation available.
AL591767 Genomic DNA No translation available.
AL627171 Genomic DNA No translation available.
BC006001 mRNA Translation: AAH06001.1 Sequence problems.
BC020794 mRNA Translation: AAH20794.2 Different initiation.
BC056687 mRNA Translation: AAH56687.1 Sequence problems.
BC064364 mRNA Translation: AAH64364.1
BC107764 mRNA Translation: AAI07765.1 Different initiation.
AF039687 mRNA Translation: AAC18036.1 Frameshift.
CCDSiCCDS9694.1 [O60524-1]
RefSeqiNP_001288661.1, NM_001301732.2
NP_004704.2, NM_004713.5
UniGeneiHs.655964

Genome annotation databases

EnsembliENST00000298310; ENSP00000298310; ENSG00000165525 [O60524-1]
GeneIDi9147
KEGGihsa:9147
UCSCiuc001wxc.4 human [O60524-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK000913 mRNA No translation available.
AK093783 mRNA Translation: BAG52763.1 Different initiation.
AK293236 mRNA Translation: BAG56774.1
AK295010 mRNA Translation: BAG58070.1 Sequence problems.
AK304695 mRNA Translation: BAG65465.1
BX640804 mRNA Translation: CAE45886.1
BX640807 mRNA Translation: CAE45889.1
BX648753 mRNA No translation available.
AL591767 Genomic DNA No translation available.
AL627171 Genomic DNA No translation available.
BC006001 mRNA Translation: AAH06001.1 Sequence problems.
BC020794 mRNA Translation: AAH20794.2 Different initiation.
BC056687 mRNA Translation: AAH56687.1 Sequence problems.
BC064364 mRNA Translation: AAH64364.1
BC107764 mRNA Translation: AAI07765.1 Different initiation.
AF039687 mRNA Translation: AAC18036.1 Frameshift.
CCDSiCCDS9694.1 [O60524-1]
RefSeqiNP_001288661.1, NM_001301732.2
NP_004704.2, NM_004713.5
UniGeneiHs.655964

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J92electron microscopy3.60u/v1-501[»]
ProteinModelPortaliO60524
SMRiO60524
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO60524, 10 interactors
MINTiO60524
STRINGi9606.ENSP00000298310

PTM databases

iPTMnetiO60524
PhosphoSitePlusiO60524

Polymorphism and mutation databases

BioMutaiNEMF

Proteomic databases

EPDiO60524
MaxQBiO60524
PaxDbiO60524
PeptideAtlasiO60524
PRIDEiO60524
ProteomicsDBi49458
49459 [O60524-2]
49460 [O60524-3]
49461 [O60524-4]
49462 [O60524-5]

Protocols and materials databases

DNASUi9147
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000298310; ENSP00000298310; ENSG00000165525 [O60524-1]
GeneIDi9147
KEGGihsa:9147
UCSCiuc001wxc.4 human [O60524-1]

Organism-specific databases

CTDi9147
DisGeNETi9147
EuPathDBiHostDB:ENSG00000165525.17
GeneCardsiNEMF
H-InvDBiHIX0011634
HGNCiHGNC:10663 NEMF
HPAiHPA004160
MIMi608378 gene
neXtProtiNX_O60524
OpenTargetsiENSG00000165525
PharmGKBiPA35593
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2030 Eukaryota
COG1293 LUCA
GeneTreeiENSGT00390000018516
HOVERGENiHBG079170
InParanoidiO60524
OMAiFEWPKNV
OrthoDBiEOG091G02MR
PhylomeDBiO60524
TreeFamiTF300515

Miscellaneous databases

ChiTaRSiNEMF human
GenomeRNAii9147
PROiPR:O60524
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000165525 Expressed in 242 organ(s), highest expression level in frontal cortex
CleanExiHS_SDCCAG1
ExpressionAtlasiO60524 baseline and differential
GenevisibleiO60524 HS

Family and domain databases

InterProiView protein in InterPro
IPR021846 DUF3441
IPR008532 DUF814
PfamiView protein in Pfam
PF11923 DUF3441, 1 hit
PF05670 DUF814, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNEMF_HUMAN
AccessioniPrimary (citable) accession number: O60524
Secondary accession number(s): A0JLQ3
, B3KSK1, B4DDL3, B4DHA9, B4E3F3, Q32Q66, Q8WW70, Q9NWG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: November 24, 2009
Last modified: October 10, 2018
This is version 142 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 14
    Human chromosome 14: entries, gene names and cross-references to MIM
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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