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Protein

Beta-1,4-galactosyltransferase 4

Gene

B4GALT4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.1 Publication

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.1 Publication
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.1 Publication

Cofactori

Mn2+By similarity

Kineticsi

  1. KM=238 µM for GlcNAc-B-S-pNP1 Publication

    Pathwayi: protein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi196ManganeseBy similarity1
    Binding sitei224UDP-alpha-D-galactoseBy similarity1
    Binding sitei256UDP-alpha-D-galactoseBy similarity1
    Metal bindingi289Manganese; via tele nitrogenBy similarity1
    Binding sitei301N-acetyl-D-glucosamineBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase
    LigandManganese, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS04504-MONOMER
    BRENDAi2.4.1.275 2681
    ReactomeiR-HSA-2022854 Keratan sulfate biosynthesis
    R-HSA-975577 N-Glycan antennae elongation
    UniPathwayi
    UPA00378

    Protein family/group databases

    CAZyiGT7 Glycosyltransferase Family 7

    Chemistry databases

    SwissLipidsiSLP:000000791

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 4 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 4
    Short name:
    Beta4Gal-T4
    Short name:
    b4Gal-T4
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4
    Including the following 2 domains:
    N-acetyllactosamine synthase (EC:2.4.1.90)
    Alternative name(s):
    Nal synthase
    Lactotriaosylceramide beta-1,4-galactosyltransferase (EC:2.4.1.275)
    Alternative name(s):
    Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
    Gene namesi
    Name:B4GALT4
    ORF Names:UNQ552/PRO1109
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 3

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000121578.12
    HGNCiHGNC:927 B4GALT4
    MIMi604015 gene
    neXtProtiNX_O60513

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
    Transmembranei13 – 38Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST26
    Topological domaini39 – 344LumenalSequence analysisAdd BLAST306

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi8702
    OpenTargetsiENSG00000121578
    PharmGKBiPA25226

    Chemistry databases

    DrugBankiDB00141 N-Acetyl-D-glucosamine

    Polymorphism and mutation databases

    BioMutaiB4GALT4

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000805421 – 344Beta-1,4-galactosyltransferase 4Add BLAST344

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi77 ↔ 118By similarity
    Disulfide bondi189 ↔ 208By similarity
    Glycosylationi220N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi335N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    EPDiO60513
    MaxQBiO60513
    PaxDbiO60513
    PeptideAtlasiO60513
    PRIDEiO60513
    ProteomicsDBi49453

    PTM databases

    iPTMnetiO60513
    PhosphoSitePlusiO60513

    Expressioni

    Tissue specificityi

    High expression in heart, placenta, kidney and pancreas; lower in brain, colon, lung, muscle, ovary, testis and uterus.

    Gene expression databases

    BgeeiENSG00000121578 Expressed in 232 organ(s), highest expression level in amniotic fluid
    CleanExiHS_B4GALT4
    ExpressionAtlasiO60513 baseline and differential
    GenevisibleiO60513 HS

    Organism-specific databases

    HPAiHPA046819
    HPA063546

    Interactioni

    Protein-protein interaction databases

    BioGridi114245, 16 interactors
    IntActiO60513, 2 interactors
    STRINGi9606.ENSP00000352144

    Structurei

    3D structure databases

    ProteinModelPortaliO60513
    SMRiO60513
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni129 – 133UDP-alpha-D-galactose bindingBy similarity5
    Regioni168 – 170UDP-alpha-D-galactose bindingBy similarity3
    Regioni195 – 196UDP-alpha-D-galactose bindingBy similarity2
    Regioni258 – 261N-acetyl-D-glucosamine bindingBy similarity4
    Regioni289 – 291UDP-alpha-D-galactose bindingBy similarity3

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG3916 Eukaryota
    ENOG410ZYYA LUCA
    GeneTreeiENSGT00760000119140
    HOGENOMiHOG000231027
    HOVERGENiHBG058334
    InParanoidiO60513
    KOiK07969
    OMAiHYAIYVI
    OrthoDBiEOG091G0P66
    PhylomeDBiO60513
    TreeFamiTF312834

    Family and domain databases

    Gene3Di3.90.550.10, 1 hit
    InterProiView protein in InterPro
    IPR003859 Galactosyl_T
    IPR027791 Galactosyl_T_C
    IPR027995 Galactosyl_T_N
    IPR029044 Nucleotide-diphossugar_trans
    PANTHERiPTHR19300 PTHR19300, 1 hit
    PfamiView protein in Pfam
    PF02709 Glyco_transf_7C, 1 hit
    PF13733 Glyco_transf_7N, 1 hit
    PRINTSiPR02050 B14GALTRFASE
    SUPFAMiSSF53448 SSF53448, 1 hit

    Sequence (1+)i

    Sequence statusi: Complete.

    This entry has 1 described isoform and 9 potential isoforms that are computationally mapped.Show allAlign All

    O60513-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MGFNLTFHLS YKFRLLLLLT LCLTVVGWAT SNYFVGAIQE IPKAKEFMAN
    60 70 80 90 100
    FHKTLILGKG KTLTNEASTK KVELDNCPSV SPYLRGQSKL IFKPDLTLEE
    110 120 130 140 150
    VQAENPKVSR GRYRPQECKA LQRVAILVPH RNREKHLMYL LEHLHPFLQR
    160 170 180 190 200
    QQLDYGIYVI HQAEGKKFNR AKLLNVGYLE ALKEENWDCF IFHDVDLVPE
    210 220 230 240 250
    NDFNLYKCEE HPKHLVVGRN STGYRLRYSG YFGGVTALSR EQFFKVNGFS
    260 270 280 290 300
    NNYWGWGGED DDLRLRVELQ RMKISRPLPE VGKYTMVFHT RDKGNEVNAE
    310 320 330 340
    RMKLLHQVSR VWRTDGLSSC SYKLVSVEHN PLYINITVDF WFGA
    Length:344
    Mass (Da):40,041
    Last modified:August 1, 1998 - v1
    Checksum:i6B7742676FE8A58B
    GO

    Computationally mapped potential isoform sequencesi

    There are 9 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    E7ETS9E7ETS9_HUMAN
    Beta-1,4-galactosyltransferase 4
    B4GALT4
    246Annotation score:
    C9J3R8C9J3R8_HUMAN
    Beta-1,4-galactosyltransferase 4
    B4GALT4
    301Annotation score:
    C9J4S5C9J4S5_HUMAN
    Beta-1,4-galactosyltransferase 4
    B4GALT4
    136Annotation score:
    C9J5S0C9J5S0_HUMAN
    Beta-1,4-galactosyltransferase 4
    B4GALT4
    118Annotation score:
    C9JA31C9JA31_HUMAN
    Beta-1,4-galactosyltransferase 4
    B4GALT4
    62Annotation score:
    C9JE00C9JE00_HUMAN
    Beta-1,4-galactosyltransferase 4
    B4GALT4
    54Annotation score:
    C9J644C9J644_HUMAN
    Beta-1,4-galactosyltransferase 4
    B4GALT4
    41Annotation score:
    C9JY35C9JY35_HUMAN
    Beta-1,4-galactosyltransferase 4
    B4GALT4
    25Annotation score:
    C9JY38C9JY38_HUMAN
    Beta-1,4-galactosyltransferase 4
    B4GALT4
    21Annotation score:

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti182L → R in AAG50147 (Ref. 4) Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_022697116Q → E3 PublicationsCorresponds to variant dbSNP:rs3764779Ensembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF038662 mRNA Translation: AAC39735.1
    AF022367 mRNA Translation: AAC72493.1
    AB024436 mRNA Translation: BAA75821.1
    AF020920 mRNA Translation: AAG50147.1
    AY359008 mRNA Translation: AAQ89367.1
    CR749555 mRNA Translation: CAH18352.1
    BC004523 mRNA Translation: AAH04523.1
    BC062618 mRNA Translation: AAH62618.1
    CCDSiCCDS2986.1
    RefSeqiNP_003769.1, NM_003778.3
    NP_997708.1, NM_212543.1
    XP_005247912.1, XM_005247855.1
    XP_006713861.1, XM_006713798.2
    XP_006713862.1, XM_006713799.2
    XP_006713863.1, XM_006713800.1
    XP_006713864.1, XM_006713801.2
    XP_011511562.1, XM_011513260.1
    UniGeneiHs.13225

    Genome annotation databases

    EnsembliENST00000359213; ENSP00000352144; ENSG00000121578
    ENST00000393765; ENSP00000377360; ENSG00000121578
    ENST00000483209; ENSP00000420161; ENSG00000121578
    GeneIDi8702
    KEGGihsa:8702
    UCSCiuc003ecg.4 human

    Keywords - Coding sequence diversityi

    Polymorphism

    Similar proteinsi

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    Beta-1,4-galactosyltransferase 4

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF038662 mRNA Translation: AAC39735.1
    AF022367 mRNA Translation: AAC72493.1
    AB024436 mRNA Translation: BAA75821.1
    AF020920 mRNA Translation: AAG50147.1
    AY359008 mRNA Translation: AAQ89367.1
    CR749555 mRNA Translation: CAH18352.1
    BC004523 mRNA Translation: AAH04523.1
    BC062618 mRNA Translation: AAH62618.1
    CCDSiCCDS2986.1
    RefSeqiNP_003769.1, NM_003778.3
    NP_997708.1, NM_212543.1
    XP_005247912.1, XM_005247855.1
    XP_006713861.1, XM_006713798.2
    XP_006713862.1, XM_006713799.2
    XP_006713863.1, XM_006713800.1
    XP_006713864.1, XM_006713801.2
    XP_011511562.1, XM_011513260.1
    UniGeneiHs.13225

    3D structure databases

    ProteinModelPortaliO60513
    SMRiO60513
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi114245, 16 interactors
    IntActiO60513, 2 interactors
    STRINGi9606.ENSP00000352144

    Chemistry databases

    DrugBankiDB00141 N-Acetyl-D-glucosamine
    SwissLipidsiSLP:000000791

    Protein family/group databases

    CAZyiGT7 Glycosyltransferase Family 7

    PTM databases

    iPTMnetiO60513
    PhosphoSitePlusiO60513

    Polymorphism and mutation databases

    BioMutaiB4GALT4

    Proteomic databases

    EPDiO60513
    MaxQBiO60513
    PaxDbiO60513
    PeptideAtlasiO60513
    PRIDEiO60513
    ProteomicsDBi49453

    Protocols and materials databases

    DNASUi8702
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000359213; ENSP00000352144; ENSG00000121578
    ENST00000393765; ENSP00000377360; ENSG00000121578
    ENST00000483209; ENSP00000420161; ENSG00000121578
    GeneIDi8702
    KEGGihsa:8702
    UCSCiuc003ecg.4 human

    Organism-specific databases

    CTDi8702
    DisGeNETi8702
    EuPathDBiHostDB:ENSG00000121578.12
    GeneCardsiB4GALT4
    HGNCiHGNC:927 B4GALT4
    HPAiHPA046819
    HPA063546
    MIMi604015 gene
    neXtProtiNX_O60513
    OpenTargetsiENSG00000121578
    PharmGKBiPA25226
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG3916 Eukaryota
    ENOG410ZYYA LUCA
    GeneTreeiENSGT00760000119140
    HOGENOMiHOG000231027
    HOVERGENiHBG058334
    InParanoidiO60513
    KOiK07969
    OMAiHYAIYVI
    OrthoDBiEOG091G0P66
    PhylomeDBiO60513
    TreeFamiTF312834

    Enzyme and pathway databases

    UniPathwayi
    UPA00378

    BioCyciMetaCyc:HS04504-MONOMER
    BRENDAi2.4.1.275 2681
    ReactomeiR-HSA-2022854 Keratan sulfate biosynthesis
    R-HSA-975577 N-Glycan antennae elongation

    Miscellaneous databases

    GeneWikiiB4GALT4
    GenomeRNAii8702
    PROiPR:O60513
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000121578 Expressed in 232 organ(s), highest expression level in amniotic fluid
    CleanExiHS_B4GALT4
    ExpressionAtlasiO60513 baseline and differential
    GenevisibleiO60513 HS

    Family and domain databases

    Gene3Di3.90.550.10, 1 hit
    InterProiView protein in InterPro
    IPR003859 Galactosyl_T
    IPR027791 Galactosyl_T_C
    IPR027995 Galactosyl_T_N
    IPR029044 Nucleotide-diphossugar_trans
    PANTHERiPTHR19300 PTHR19300, 1 hit
    PfamiView protein in Pfam
    PF02709 Glyco_transf_7C, 1 hit
    PF13733 Glyco_transf_7N, 1 hit
    PRINTSiPR02050 B14GALTRFASE
    SUPFAMiSSF53448 SSF53448, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiB4GT4_HUMAN
    AccessioniPrimary (citable) accession number: O60513
    Secondary accession number(s): Q68D68, Q9BSW3, Q9C078
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: August 1, 1998
    Last modified: November 7, 2018
    This is version 171 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Human chromosome 3
      Human chromosome 3: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PATHWAY comments
      Index of metabolic and biosynthesis pathways
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