UniProtKB - O60512 (B4GT3_HUMAN)
Protein
Beta-1,4-galactosyltransferase 3
Gene
B4GALT3
Organism
Homo sapiens (Human)
Status
Functioni
Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.2 Publications
Catalytic activityi
- an N-acetyl-β-D-glucosaminyl derivative + UDP-α-D-galactose = a β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl derivative + H+ + UDP2 PublicationsEC:2.4.1.382 PublicationsThis reaction proceeds in the forward2 Publications direction.
- N-acetyl-D-glucosamine + UDP-α-D-galactose = β-D-galactosyl-(1→4)-N-acetyl-D-glucosamine + H+ + UDP1 PublicationEC:2.4.1.901 PublicationThis reaction proceeds in the forward1 Publication direction.
- a β-D-GlcNAc-(1→3)-β-D-Gal-(1→4)-β-D-Glc-(1↔1)-Cer(d18:1(4E)) + UDP-α-D-galactose = a neolactoside nLc4Cer(d18:1(4E)) + H+ + UDP1 PublicationEC:2.4.1.2751 PublicationThis reaction proceeds in the forward1 Publication direction.
- β-D-glucosylceramide + UDP-α-D-galactose = β-D-galactosyl-(1→4)-β-D-glucosyl-(1↔1)-ceramide + H+ + UDP1 PublicationThis reaction proceeds in the forward1 Publication direction.
- a neolactoside IV3-β-GlcNAc-nLc4Cer + UDP-α-D-galactose = β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl-(1→3)-β-D-galactosyl-(1→4)-N-acetyl-β-D-glucosaminyl-(1→3)-β-D-galactosyl-(1→4)-β-D-glucosyl-(1↔1ʼ)-ceramide + H+ + UDP1 PublicationThis reaction proceeds in the forward1 Publication direction.
Cofactori
Mn2+By similarity
Kineticsi
- KM=63 µM for GlcNAc-B-S-pNP1 Publication
- KM=0.084 mM for UDP-galactose1 Publication
- KM=0.58 mM for benzyl-beta-D-GlcNAc1 Publication
- KM=13.1 mM for D-GlcNAc1 Publication
- Vmax=64.35 pmol/min/mg enzyme towards UDP-galactose1 Publication
- Vmax=66.25 pmol/min/mg enzyme towards for benzyl-beta-D-GlcNAc1 Publication
- Vmax=60.85 pmol/min/mg enzyme towards D-GlcNAc1 Publication
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.2 PublicationsView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 197 | ManganeseBy similarity | 1 | |
Binding sitei | 226 | UDP-alpha-D-galactoseBy similarity | 1 | |
Binding sitei | 258 | UDP-alpha-D-galactoseBy similarity | 1 | |
Metal bindingi | 291 | Manganese; via tele nitrogenBy similarity | 1 | |
Binding sitei | 303 | N-acetyl-D-glucosamineBy similarity | 1 |
GO - Molecular functioni
- beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: UniProtKB-EC
- galactosyltransferase activity Source: BHF-UCL
- metal ion binding Source: UniProtKB-KW
- N-acetyllactosamine synthase activity Source: UniProtKB-EC
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- galactosylceramide biosynthetic process Source: BHF-UCL
- keratan sulfate biosynthetic process Source: Reactome
- protein glycosylation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Ligand | Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | MetaCyc:HS08336-MONOMER |
PathwayCommonsi | O60512 |
Reactomei | R-HSA-2022854, Keratan sulfate biosynthesis R-HSA-975577, N-Glycan antennae elongation |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GT7, Glycosyltransferase Family 7 |
Chemistry databases
SwissLipidsi | SLP:000000790 |
Names & Taxonomyi
Protein namesi | Recommended name: Beta-1,4-galactosyltransferase 3Curated (EC:2.4.1.-1 Publication)Short name: Beta-1,4-GalTase 31 Publication Short name: Beta4Gal-T3 Short name: b4Gal-T31 Publication Alternative name(s): Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase1 Publication (EC:2.4.1.381 Publication) N-acetyllactosamine synthase1 Publication (EC:2.4.1.901 Publication) Nal synthase Neolactotriaosylceramide beta-1,4-galactosyltransferase1 Publication (EC:2.4.1.2751 Publication) UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3 UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3 |
Gene namesi | Name:B4GALT3Imported |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
EuPathDBi | HostDB:ENSG00000158850.14 |
HGNCi | HGNC:926, B4GALT3 |
MIMi | 604014, gene |
neXtProti | NX_O60512 |
Subcellular locationi
Golgi apparatus
Note: Trans cisternae of Golgi stack.
Cytosol
- cytosol Source: HPA
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: HPA
- Golgi cisterna membrane Source: UniProtKB-SubCell
- Golgi membrane Source: Reactome
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 10 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 11 – 31 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 32 – 393 | LumenalSequence analysisAdd BLAST | 362 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 8703 |
OpenTargetsi | ENSG00000158850 |
PharmGKBi | PA25225 |
Miscellaneous databases
Pharosi | O60512, Tbio |
Polymorphism and mutation databases
BioMutai | B4GALT3 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080537 | 1 – 393 | Beta-1,4-galactosyltransferase 3Add BLAST | 393 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 57 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 77 ↔ 119 | By similarity | ||
Glycosylationi | 166 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 190 ↔ 209 | By similarity | ||
Glycosylationi | 337 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 385 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
EPDi | O60512 |
jPOSTi | O60512 |
MassIVEi | O60512 |
MaxQBi | O60512 |
PaxDbi | O60512 |
PeptideAtlasi | O60512 |
PRIDEi | O60512 |
ProteomicsDBi | 49451 [O60512-1] 49452 [O60512-2] |
PTM databases
GlyConnecti | 1032, 6 N-Linked glycans (2 sites) |
GlyGeni | O60512, 5 sites |
iPTMneti | O60512 |
PhosphoSitePlusi | O60512 |
SwissPalmi | O60512 |
Expressioni
Tissue specificityi
Found in various tissues. Highest expression in placenta, prostate, testis, ovary, intestine and muscle, and in fetal brain.1 Publication
Gene expression databases
Bgeei | ENSG00000158850, Expressed in granulocyte and 233 other tissues |
ExpressionAtlasi | O60512, baseline and differential |
Genevisiblei | O60512, HS |
Organism-specific databases
HPAi | ENSG00000158850, Low tissue specificity |
Interactioni
Protein-protein interaction databases
BioGRIDi | 114246, 62 interactors |
IntActi | O60512, 24 interactors |
STRINGi | 9606.ENSP00000480428 |
Miscellaneous databases
RNActi | O60512, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 130 – 134 | UDP-alpha-D-galactose bindingBy similarity | 5 | |
Regioni | 169 – 171 | UDP-alpha-D-galactose bindingBy similarity | 3 | |
Regioni | 196 – 197 | UDP-alpha-D-galactose bindingBy similarity | 2 | |
Regioni | 260 – 263 | N-acetyl-D-glucosamine bindingBy similarity | 4 | |
Regioni | 291 – 293 | UDP-alpha-D-galactose bindingBy similarity | 3 |
Sequence similaritiesi
Belongs to the glycosyltransferase 7 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3916, Eukaryota |
GeneTreei | ENSGT00940000158549 |
HOGENOMi | CLU_044391_1_2_1 |
InParanoidi | O60512 |
OMAi | NIGEDPH |
OrthoDBi | 1201618at2759 |
PhylomeDBi | O60512 |
TreeFami | TF312834 |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR003859, Galactosyl_T IPR027791, Galactosyl_T_C IPR027995, Galactosyl_T_N IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR19300, PTHR19300, 1 hit |
Pfami | View protein in Pfam PF02709, Glyco_transf_7C, 1 hit PF13733, Glyco_transf_7N, 1 hit |
PRINTSi | PR02050, B14GALTRFASE |
SUPFAMi | SSF53448, SSF53448, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: O60512-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DQGPTFDYSH
60 70 80 90 100
PRDVYSNLSH LPGAPGGPPA PQGLPYCPER SPLLVGPVSV SFSPVPSLAE
110 120 130 140 150
IVERNPRVEP GGRYRPAGCE PRSRTAIIVP HRAREHHLRL LLYHLHPFLQ
160 170 180 190 200
RQQLAYGIYV IHQAGNGTFN RAKLLNVGVR EALRDEEWDC LFLHDVDLLP
210 220 230 240 250
ENDHNLYVCD PRGPRHVAVA MNKFGYSLPY PQYFGGVSAL TPDQYLKMNG
260 270 280 290 300
FPNEYWGWGG EDDDIATRVR LAGMKISRPP TSVGHYKMVK HRGDKGNEEN
310 320 330 340 350
PHRFDLLVRT QNSWTQDGMN SLTYQLLARE LGPLYTNITA DIGTDPRGPR
360 370 380 390
APSGPRYPPG SSQAFRQEML QRRPPARPGP LSTANHTALR GSH
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 64 | A → R in AAC39734 (PubMed:9597550).Curated | 1 | |
Sequence conflicti | 112 | G → A in AAC39734 (PubMed:9597550).Curated | 1 | |
Sequence conflicti | 223 | K → S in AAC39734 (PubMed:9597550).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_014106 | 116 – 145 | PAGCE…LLYHL → PAALPPAPLLAAPAACLWHL CHPPGWKWNI in isoform 2. 1 PublicationAdd BLAST | 30 | |
Alternative sequenceiVSP_014107 | 146 – 393 | Missing in isoform 2. 1 PublicationAdd BLAST | 248 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y12509 mRNA Translation: CAA73111.1 AF038661 mRNA Translation: AAC39734.1 AB024435 mRNA Translation: BAA75820.1 AL590714 Genomic DNA No translation available. AK023311 mRNA Translation: BAB14520.1 CH471121 Genomic DNA Translation: EAW52628.1 CH471121 Genomic DNA Translation: EAW52629.1 CH471121 Genomic DNA Translation: EAW52630.1 CH471121 Genomic DNA Translation: EAW52631.1 CH471121 Genomic DNA Translation: EAW52632.1 BC000276 mRNA Translation: AAH00276.1 BC006099 mRNA Translation: AAH06099.1 BC009985 mRNA Translation: AAH09985.1 |
CCDSi | CCDS1222.1 [O60512-1] |
RefSeqi | NP_001186802.1, NM_001199873.1 [O60512-1] NP_001186803.1, NM_001199874.1 [O60512-1] NP_003770.1, NM_003779.3 [O60512-1] XP_005245623.1, XM_005245566.1 [O60512-1] |
Genome annotation databases
Ensembli | ENST00000319769; ENSP00000320965; ENSG00000158850 [O60512-1] ENST00000367998; ENSP00000356977; ENSG00000158850 [O60512-1] ENST00000622395; ENSP00000480428; ENSG00000158850 [O60512-1] |
GeneIDi | 8703 |
KEGGi | hsa:8703 |
UCSCi | uc001fyq.3, human [O60512-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
Functional Glycomics Gateway - GTase Beta-1,4-galactosyltransferase 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Y12509 mRNA Translation: CAA73111.1 AF038661 mRNA Translation: AAC39734.1 AB024435 mRNA Translation: BAA75820.1 AL590714 Genomic DNA No translation available. AK023311 mRNA Translation: BAB14520.1 CH471121 Genomic DNA Translation: EAW52628.1 CH471121 Genomic DNA Translation: EAW52629.1 CH471121 Genomic DNA Translation: EAW52630.1 CH471121 Genomic DNA Translation: EAW52631.1 CH471121 Genomic DNA Translation: EAW52632.1 BC000276 mRNA Translation: AAH00276.1 BC006099 mRNA Translation: AAH06099.1 BC009985 mRNA Translation: AAH09985.1 |
CCDSi | CCDS1222.1 [O60512-1] |
RefSeqi | NP_001186802.1, NM_001199873.1 [O60512-1] NP_001186803.1, NM_001199874.1 [O60512-1] NP_003770.1, NM_003779.3 [O60512-1] XP_005245623.1, XM_005245566.1 [O60512-1] |
3D structure databases
SMRi | O60512 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 114246, 62 interactors |
IntActi | O60512, 24 interactors |
STRINGi | 9606.ENSP00000480428 |
Chemistry databases
SwissLipidsi | SLP:000000790 |
Protein family/group databases
CAZyi | GT7, Glycosyltransferase Family 7 |
PTM databases
GlyConnecti | 1032, 6 N-Linked glycans (2 sites) |
GlyGeni | O60512, 5 sites |
iPTMneti | O60512 |
PhosphoSitePlusi | O60512 |
SwissPalmi | O60512 |
Polymorphism and mutation databases
BioMutai | B4GALT3 |
Proteomic databases
EPDi | O60512 |
jPOSTi | O60512 |
MassIVEi | O60512 |
MaxQBi | O60512 |
PaxDbi | O60512 |
PeptideAtlasi | O60512 |
PRIDEi | O60512 |
ProteomicsDBi | 49451 [O60512-1] 49452 [O60512-2] |
Protocols and materials databases
Antibodypediai | 2534, 206 antibodies |
DNASUi | 8703 |
Genome annotation databases
Ensembli | ENST00000319769; ENSP00000320965; ENSG00000158850 [O60512-1] ENST00000367998; ENSP00000356977; ENSG00000158850 [O60512-1] ENST00000622395; ENSP00000480428; ENSG00000158850 [O60512-1] |
GeneIDi | 8703 |
KEGGi | hsa:8703 |
UCSCi | uc001fyq.3, human [O60512-1] |
Organism-specific databases
CTDi | 8703 |
DisGeNETi | 8703 |
EuPathDBi | HostDB:ENSG00000158850.14 |
GeneCardsi | B4GALT3 |
HGNCi | HGNC:926, B4GALT3 |
HPAi | ENSG00000158850, Low tissue specificity |
MIMi | 604014, gene |
neXtProti | NX_O60512 |
OpenTargetsi | ENSG00000158850 |
PharmGKBi | PA25225 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG3916, Eukaryota |
GeneTreei | ENSGT00940000158549 |
HOGENOMi | CLU_044391_1_2_1 |
InParanoidi | O60512 |
OMAi | NIGEDPH |
OrthoDBi | 1201618at2759 |
PhylomeDBi | O60512 |
TreeFami | TF312834 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BioCyci | MetaCyc:HS08336-MONOMER |
PathwayCommonsi | O60512 |
Reactomei | R-HSA-2022854, Keratan sulfate biosynthesis R-HSA-975577, N-Glycan antennae elongation |
Miscellaneous databases
BioGRID-ORCSi | 8703, 17 hits in 843 CRISPR screens |
ChiTaRSi | B4GALT3, human |
GeneWikii | B4GALT3 |
GenomeRNAii | 8703 |
Pharosi | O60512, Tbio |
PROi | PR:O60512 |
RNActi | O60512, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000158850, Expressed in granulocyte and 233 other tissues |
ExpressionAtlasi | O60512, baseline and differential |
Genevisiblei | O60512, HS |
Family and domain databases
Gene3Di | 3.90.550.10, 1 hit |
InterProi | View protein in InterPro IPR003859, Galactosyl_T IPR027791, Galactosyl_T_C IPR027995, Galactosyl_T_N IPR029044, Nucleotide-diphossugar_trans |
PANTHERi | PTHR19300, PTHR19300, 1 hit |
Pfami | View protein in Pfam PF02709, Glyco_transf_7C, 1 hit PF13733, Glyco_transf_7N, 1 hit |
PRINTSi | PR02050, B14GALTRFASE |
SUPFAMi | SSF53448, SSF53448, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | B4GT3_HUMAN | |
Accessioni | O60512Primary (citable) accession number: O60512 Secondary accession number(s): D3DVG3 Q9H8T2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 21, 2001 |
Last sequence update: | June 21, 2005 | |
Last modified: | December 2, 2020 | |
This is version 183 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM