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UniProtKB - O60512 (B4GT3_HUMAN)

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Protein

Beta-1,4-galactosyltransferase 3

Gene

B4GALT3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactori

Mn2+By similarity

Kineticsi

  1. KM=63 µM for GlcNAc-B-S-pNP1 Publication

    Pathwayi: protein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Metal bindingi197ManganeseBy similarity1
    Binding sitei226UDP-alpha-D-galactoseBy similarity1
    Binding sitei258UDP-alpha-D-galactoseBy similarity1
    Metal bindingi291Manganese; via tele nitrogenBy similarity1
    Binding sitei303N-acetyl-D-glucosamineBy similarity1

    GO - Molecular functioni

    View the complete GO annotation on QuickGO ...

    GO - Biological processi

    View the complete GO annotation on QuickGO ...

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase
    LigandManganese, Metal-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:HS08336-MONOMER
    ReactomeiR-HSA-2022854 Keratan sulfate biosynthesis
    R-HSA-975577 N-Glycan antennae elongation
    UniPathwayiUPA00378

    Protein family/group databases

    CAZyiGT7 Glycosyltransferase Family 7

    Chemistry databases

    SwissLipidsiSLP:000000790

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 3 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 3
    Short name:
    Beta4Gal-T3
    Short name:
    b4Gal-T3
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 3
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 3
    Including the following 3 domains:
    N-acetyllactosamine synthase (EC:2.4.1.90)
    Alternative name(s):
    Nal synthase
    Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
    Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
    Gene namesi
    Name:B4GALT3
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
    Proteomesi
    • UP000005640 Componenti: Chromosome 1

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000158850.14
    HGNCiHGNC:926 B4GALT3
    MIMi604014 gene
    neXtProtiNX_O60512

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 10CytoplasmicSequence analysis10
    Transmembranei11 – 31Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
    Topological domaini32 – 393LumenalSequence analysisAdd BLAST362

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi8703
    OpenTargetsiENSG00000158850
    PharmGKBiPA25225

    Chemistry databases

    DrugBankiDB00141 N-Acetyl-D-glucosamine

    Polymorphism and mutation databases

    BioMutaiB4GALT3

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00000805371 – 393Beta-1,4-galactosyltransferase 3Add BLAST393

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi57N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi77 ↔ 119By similarity
    Glycosylationi166N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi190 ↔ 209By similarity
    Glycosylationi337N-linked (GlcNAc...) asparagineSequence analysis1
    Glycosylationi385N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    EPDiO60512
    MaxQBiO60512
    PaxDbiO60512
    PeptideAtlasiO60512
    PRIDEiO60512
    ProteomicsDBi49451
    49452 [O60512-2]

    PTM databases

    iPTMnetiO60512
    PhosphoSitePlusiO60512

    Expressioni

    Tissue specificityi

    Found in various tissues. Highest expression in placenta, prostate, testis, ovary, intestine and muscle, and in fetal brain.

    Gene expression databases

    BgeeiENSG00000158850
    CleanExiHS_B4GALT3
    GenevisibleiO60512 HS

    Organism-specific databases

    HPAiHPA010793

    Interactioni

    Protein-protein interaction databases

    BioGridi114246, 35 interactors
    IntActiO60512, 6 interactors
    STRINGi9606.ENSP00000320965

    Structurei

    3D structure databases

    ProteinModelPortaliO60512
    SMRiO60512
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni130 – 134UDP-alpha-D-galactose bindingBy similarity5
    Regioni169 – 171UDP-alpha-D-galactose bindingBy similarity3
    Regioni196 – 197UDP-alpha-D-galactose bindingBy similarity2
    Regioni260 – 263N-acetyl-D-glucosamine bindingBy similarity4
    Regioni291 – 293UDP-alpha-D-galactose bindingBy similarity3

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG3916 Eukaryota
    ENOG410ZYYA LUCA
    GeneTreeiENSGT00760000119140
    HOGENOMiHOG000231027
    HOVERGENiHBG058334
    InParanoidiO60512
    KOiK07968
    OMAiFDYSRPH
    OrthoDBiEOG091G0P66
    PhylomeDBiO60512
    TreeFamiTF312834

    Family and domain databases

    Gene3Di3.90.550.10, 1 hit
    InterProiView protein in InterPro
    IPR003859 Galactosyl_T
    IPR027791 Galactosyl_T_C
    IPR027995 Galactosyl_T_N
    IPR029044 Nucleotide-diphossugar_trans
    PANTHERiPTHR19300 PTHR19300, 1 hit
    PfamiView protein in Pfam
    PF02709 Glyco_transf_7C, 1 hit
    PF13733 Glyco_transf_7N, 1 hit
    PRINTSiPR02050 B14GALTRFASE
    SUPFAMiSSF53448 SSF53448, 1 hit

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

    Isoform 1 (identifier: O60512-1) [UniParc]FASTAAdd to basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

            10         20         30         40         50
    MLRRLLERPC TLALLVGSQL AVMMYLSLGG FRSLSALFGR DQGPTFDYSH 
            60         70         80         90        100
    PRDVYSNLSH LPGAPGGPPA PQGLPYCPER SPLLVGPVSV SFSPVPSLAE 
           110        120        130        140        150
    IVERNPRVEP GGRYRPAGCE PRSRTAIIVP HRAREHHLRL LLYHLHPFLQ 
           160        170        180        190        200
    RQQLAYGIYV IHQAGNGTFN RAKLLNVGVR EALRDEEWDC LFLHDVDLLP 
           210        220        230        240        250
    ENDHNLYVCD PRGPRHVAVA MNKFGYSLPY PQYFGGVSAL TPDQYLKMNG 
           260        270        280        290        300
    FPNEYWGWGG EDDDIATRVR LAGMKISRPP TSVGHYKMVK HRGDKGNEEN 
           310        320        330        340        350
    PHRFDLLVRT QNSWTQDGMN SLTYQLLARE LGPLYTNITA DIGTDPRGPR 
           360        370        380        390 
    APSGPRYPPG SSQAFRQEML QRRPPARPGP LSTANHTALR GSH
    Length:393
    Mass (Da):43,928
    Last modified:June 21, 2005 - v2
    Checksum:iCA0BF955F95ED1F4
    GO
    Isoform 2 (identifier: O60512-2) [UniParc]FASTAAdd to basket

    The sequence of this isoform differs from the canonical sequence as follows:
         116-145: PAGCEPRSRTAIIVPHRAREHHLRLLLYHL → PAALPPAPLLAAPAACLWHLCHPPGWKWNI
         146-393: Missing.

    Note: No experimental confirmation available.
    Show »
    Length:145
    Mass (Da):15,654
    Checksum:i4231F9B50756A99C
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti64A → R in AAC39734 (PubMed:9597550).Curated1
    Sequence conflicti112G → A in AAC39734 (PubMed:9597550).Curated1
    Sequence conflicti223K → S in AAC39734 (PubMed:9597550).Curated1

    Alternative sequence

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Alternative sequenceiVSP_014106116 – 145PAGCE…LLYHL → PAALPPAPLLAAPAACLWHL CHPPGWKWNI in isoform 2. 1 PublicationAdd BLAST30
    Alternative sequenceiVSP_014107146 – 393Missing in isoform 2. 1 PublicationAdd BLAST248

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    		Y12509 mRNA Translation: CAA73111.1
    AF038661 mRNA Translation: AAC39734.1
    AB024435 mRNA Translation: BAA75820.1
    AL590714 Genomic DNA No translation available.
    AK023311 mRNA Translation: BAB14520.1
    CH471121 Genomic DNA Translation: EAW52628.1
    CH471121 Genomic DNA Translation: EAW52629.1
    CH471121 Genomic DNA Translation: EAW52630.1
    CH471121 Genomic DNA Translation: EAW52631.1
    CH471121 Genomic DNA Translation: EAW52632.1
    BC000276 mRNA Translation: AAH00276.1
    BC006099 mRNA Translation: AAH06099.1
    BC009985 mRNA Translation: AAH09985.1
    CCDSiCCDS1222.1 [O60512-1]
    RefSeqiNP_001186802.1, NM_001199873.1 [O60512-1]
    NP_001186803.1, NM_001199874.1 [O60512-1]
    NP_003770.1, NM_003779.3 [O60512-1]
    XP_005245623.1, XM_005245566.1 [O60512-1]
    UniGeneiHs.321231
    Hs.741821

    Genome annotation databases

    EnsembliENST00000319769; ENSP00000320965; ENSG00000158850 [O60512-1]
    ENST00000367998; ENSP00000356977; ENSG00000158850 [O60512-1]
    ENST00000622395; ENSP00000480428; ENSG00000158850 [O60512-1]
    GeneIDi8703
    KEGGihsa:8703
    UCSCiuc001fyq.3 human [O60512-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Similar proteinsi

    Entry informationi

    Entry nameiB4GT3_HUMAN
    AccessioniPrimary (citable) accession number: O60512
    Secondary accession number(s): D3DVG3
    , O60910, Q9BPZ4, Q9H8T2
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: June 21, 2005
    Last modified: June 20, 2018
    This is version 165 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

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