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Protein

Protein-tyrosine sulfotransferase 1

Gene

TPST1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the O-sulfation of tyrosine residues within acidic motifs of polypeptides, using 3'-phosphoadenylyl sulfate (PAPS) as cosubstrate.3 Publications

Catalytic activityi

3'-phosphoadenylyl sulfate + protein tyrosine = adenosine 3',5'-bisphosphate + protein tyrosine-O-sulfate.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei100Proton donor/acceptorBy similarity1
Sitei159Transition state stabilizerBy similarity1
Binding sitei184PAPSCombined sources1 Publication1
Binding sitei192PAPSCombined sources1 Publication1
Binding sitei196PAPSCombined sources1 Publication1
Binding sitei239PAPSCombined sources1 Publication1
Sitei286Transition state stabilizerBy similarity1
Binding sitei301PAPSCombined sources1 Publication1

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB
  • protein-tyrosine sulfotransferase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase

Enzyme and pathway databases

BioCyciMetaCyc:HS10030-MONOMER
BRENDAi2.8.2.20 2681
ReactomeiR-HSA-156584 Cytosolic sulfonation of small molecules

Names & Taxonomyi

Protein namesi
Recommended name:
Protein-tyrosine sulfotransferase 1 (EC:2.8.2.203 Publications)
Alternative name(s):
Tyrosylprotein sulfotransferase 1
Short name:
TPST-1
Gene namesi
Name:TPST1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000169902.13
HGNCiHGNC:12020 TPST1
MIMi603125 gene
neXtProtiNX_O60507

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 8CytoplasmicSequence analysis8
Transmembranei9 – 25Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST17
Topological domaini26 – 370LumenalSequence analysisAdd BLAST345

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi60N → A: Loss of one glycosylation site. Loss of N-glycosylation; when associated with A-262. 1 Publication1
Mutagenesisi262N → A: Loss of one glycosylation site. Loss of N-glycosylation; when associated with A-60. 1 Publication1

Organism-specific databases

DisGeNETi8460
OpenTargetsiENSG00000169902
PharmGKBiPA36699

Polymorphism and mutation databases

BioMutaiTPST1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001898261 – 370Protein-tyrosine sulfotransferase 1Add BLAST370

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi60N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi97 ↔ 1571 Publication
Disulfide bondi226 ↔ 2341 Publication
Glycosylationi262N-linked (GlcNAc...) asparagine1 Publication1

Post-translational modificationi

N-glycosylated.2 Publications

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

EPDiO60507
MaxQBiO60507
PaxDbiO60507
PeptideAtlasiO60507
PRIDEiO60507
ProteomicsDBi49449

PTM databases

iPTMnetiO60507
PhosphoSitePlusiO60507

Expressioni

Tissue specificityi

Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.1 Publication

Gene expression databases

BgeeiENSG00000169902 Expressed in 195 organ(s), highest expression level in urinary bladder
CleanExiHS_TPST1
ExpressionAtlasiO60507 baseline and differential
GenevisibleiO60507 HS

Organism-specific databases

HPAiHPA047415
HPA061837

Interactioni

Subunit structurei

Homodimer (PubMed:16859706, PubMed:28821720). Can also form heterodimers with TPST2 (PubMed:25660941).3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi114038, 6 interactors
STRINGi9606.ENSP00000302413

Structurei

Secondary structure

1370
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO60507
SMRiO60507
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni79 – 83PAPS bindingCombined sources1 Publication5
Regioni102 – 106Interaction with peptide substrate1 Publication5
Regioni286 – 295PAPS bindingCombined sources1 Publication10

Sequence similaritiesi

Belongs to the protein sulfotransferase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3988 Eukaryota
ENOG410XQ2X LUCA
GeneTreeiENSGT00390000006030
HOGENOMiHOG000253020
HOVERGENiHBG001270
InParanoidiO60507
KOiK01021
OMAiYLGRHAM
OrthoDBiEOG091G09YJ
PhylomeDBiO60507
TreeFamiTF312910

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR026634 TPST
PANTHERiPTHR12788 PTHR12788, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

O60507-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVGKLKQNLL LACLVISSVT VFYLGQHAME CHHRIEERSQ PVKLESTRTT
60 70 80 90 100
VRTGLDLKAN KTFAYHKDMP LIFIGGVPRS GTTLMRAMLD AHPDIRCGEE
110 120 130 140 150
TRVIPRILAL KQMWSRSSKE KIRLDEAGVT DEVLDSAMQA FLLEIIVKHG
160 170 180 190 200
EPAPYLCNKD PFALKSLTYL SRLFPNAKFL LMVRDGRASV HSMISRKVTI
210 220 230 240 250
AGFDLNSYRD CLTKWNRAIE TMYNQCMEVG YKKCMLVHYE QLVLHPERWM
260 270 280 290 300
RTLLKFLQIP WNHSVLHHEE MIGKAGGVSL SKVERSTDQV IKPVNVGALS
310 320 330 340 350
KWVGKIPPDV LQDMAVIAPM LAKLGYDPYA NPPNYGKPDP KIIENTRRVY
360 370
KGEFQLPDFL KEKPQTEQVE
Length:370
Mass (Da):42,188
Last modified:August 1, 1998 - v1
Checksum:iFB9EB341AFE36407
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9J3I4C9J3I4_HUMAN
Protein-tyrosine sulfotransferase
TPST1
140Annotation score:
C9K0F3C9K0F3_HUMAN
Protein-tyrosine sulfotransferase
TPST1
41Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038009 mRNA Translation: AAC13552.1
AK313098 mRNA Translation: BAG35922.1
CR542060 mRNA Translation: CAG46857.1
CR542080 mRNA Translation: CAG46877.1
CH236961 Genomic DNA Translation: EAL23739.1
CH471140 Genomic DNA Translation: EAX07937.1
BC013188 mRNA Translation: AAH13188.1
CCDSiCCDS5533.1
RefSeqiNP_003587.1, NM_003596.3
XP_016868214.1, XM_017012725.1
XP_016868215.1, XM_017012726.1
XP_016868216.1, XM_017012727.1
UniGeneiHs.421194

Genome annotation databases

EnsembliENST00000304842; ENSP00000302413; ENSG00000169902
GeneIDi8460
KEGGihsa:8460
UCSCiuc003tuw.4 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038009 mRNA Translation: AAC13552.1
AK313098 mRNA Translation: BAG35922.1
CR542060 mRNA Translation: CAG46857.1
CR542080 mRNA Translation: CAG46877.1
CH236961 Genomic DNA Translation: EAL23739.1
CH471140 Genomic DNA Translation: EAX07937.1
BC013188 mRNA Translation: AAH13188.1
CCDSiCCDS5533.1
RefSeqiNP_003587.1, NM_003596.3
XP_016868214.1, XM_017012725.1
XP_016868215.1, XM_017012726.1
XP_016868216.1, XM_017012727.1
UniGeneiHs.421194

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5WRIX-ray1.60A/B43-341[»]
5WRJX-ray2.31A/B/C/D43-341[»]
ProteinModelPortaliO60507
SMRiO60507
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114038, 6 interactors
STRINGi9606.ENSP00000302413

PTM databases

iPTMnetiO60507
PhosphoSitePlusiO60507

Polymorphism and mutation databases

BioMutaiTPST1

Proteomic databases

EPDiO60507
MaxQBiO60507
PaxDbiO60507
PeptideAtlasiO60507
PRIDEiO60507
ProteomicsDBi49449

Protocols and materials databases

DNASUi8460
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000304842; ENSP00000302413; ENSG00000169902
GeneIDi8460
KEGGihsa:8460
UCSCiuc003tuw.4 human

Organism-specific databases

CTDi8460
DisGeNETi8460
EuPathDBiHostDB:ENSG00000169902.13
GeneCardsiTPST1
HGNCiHGNC:12020 TPST1
HPAiHPA047415
HPA061837
MIMi603125 gene
neXtProtiNX_O60507
OpenTargetsiENSG00000169902
PharmGKBiPA36699
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3988 Eukaryota
ENOG410XQ2X LUCA
GeneTreeiENSGT00390000006030
HOGENOMiHOG000253020
HOVERGENiHBG001270
InParanoidiO60507
KOiK01021
OMAiYLGRHAM
OrthoDBiEOG091G09YJ
PhylomeDBiO60507
TreeFamiTF312910

Enzyme and pathway databases

BioCyciMetaCyc:HS10030-MONOMER
BRENDAi2.8.2.20 2681
ReactomeiR-HSA-156584 Cytosolic sulfonation of small molecules

Miscellaneous databases

ChiTaRSiTPST1 human
GenomeRNAii8460
PROiPR:O60507
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000169902 Expressed in 195 organ(s), highest expression level in urinary bladder
CleanExiHS_TPST1
ExpressionAtlasiO60507 baseline and differential
GenevisibleiO60507 HS

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR026634 TPST
PANTHERiPTHR12788 PTHR12788, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTPST1_HUMAN
AccessioniPrimary (citable) accession number: O60507
Secondary accession number(s): A4D2M0, Q6FGM7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: November 7, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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