OGA

Functionⁱ

Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).5 Publications

Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.1 Publication

Caution

The mouse and rat orthologs were initially identified as bi-functional proteins containing an N-terminal domain with O-GlcNAcase activity and a C-terminal domain with histone acetyltransferase activity. The histone acetyltransferase activity was detected only when the protein was expressed in mammalian cells, but not when expressed in bacterial cells, suggesting that the histone acetyltransferase activity might be due to the presence of a contaminant. Comparison of the human protein with a bacterial putative acetyltransferase (AC Q2CEE2) shows that the residues important for acetyl-CoA binding are not conserved, and that the residues proposed to be important for histone acetyltransferase activity are not in a position where they could participate in catalysis. Characterization of the human protein shows that it does not bind acetyl-CoA and therefore cannot have acetyltransferase activity.1 Publication1 Publication

Catalytic activityⁱ

Activity regulationⁱ

Inhibited by N-acetylglucosamine and not N-acetylgalactosamine.1 Publication

Kineticsⁱ

K_M=
1.1 mM for pNP-GlcNAc
1 Publication
Manual assertion based on experiment inⁱ
- Ref.9
  "Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic beta-N-acetylglucosaminidase, O-GlcNAcase."
  Wells L., Gao Y., Mahoney J.A., Vosseller K., Chen C., Rosen A., Hart G.W.
  J. Biol. Chem. 277:1755-1761(2002) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, PTM, SUBCELLULAR LOCATION.

V_max=
652 µmol/min/mg enzyme with pNP-GLcNAc as substrate
1 Publication
Manual assertion based on experiment inⁱ
- Ref.9
  "Dynamic O-glycosylation of nuclear and cytosolic proteins: further characterization of the nucleocytoplasmic beta-N-acetylglucosaminidase, O-GlcNAcase."
  Wells L., Gao Y., Mahoney J.A., Vosseller K., Chen C., Rosen A., Hart G.W.
  J. Biol. Chem. 277:1755-1761(2002) [PubMed] [Europe PMC] [Abstract]
  Cited for: FUNCTION, CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, PTM, SUBCELLULAR LOCATION.

pH dependenceⁱ

Optimum pH is 5.7-7.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	67	Substrate; via carbonyl oxygenBy similarity	1
Binding siteⁱ	98	SubstrateBy similarity	1
Binding siteⁱ	174	SubstrateBy similarity	1
Active siteⁱ	175	Proton donor1 Publication	1
Binding siteⁱ	219	SubstrateBy similarity	1
Binding siteⁱ	285	SubstrateBy similarity	1
Binding siteⁱ	313	SubstrateBy similarity	1

GO - Molecular functionⁱ

[protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine/L-threonine O-N-acetyl-alpha-D-glucosaminase activity Source: UniProtKB-EC
[protein]-3-O-(N-acetyl-D-glucosaminyl)-L-serine O-N-acetyl-alpha-D-glucosaminase activity Source: UniProtKB-EC
[protein]-3-O-(N-acetyl-D-glucosaminyl)-L-threonine O-N-acetyl-alpha-D-glucosaminase activity Source: UniProtKB-EC
beta-N-acetylglucosaminidase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 11148210
hyalurononglucosaminidase activity
Source: ProtInc
Traceable author statementⁱ
- 9811929

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

glycoprotein catabolic process
Source: ProtInc
Traceable author statementⁱ
- 9811929
glycoprotein metabolic process
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
N-acetylglucosamine metabolic process
Source: UniProtKB
Inferred from direct assayⁱ
- 11148210
protein deglycosylation
Source: UniProtKB
Inferred from direct assayⁱ
- 11148210
protein O-linked glycosylation
Source: ParkinsonsUK-UCL
Non-traceable author statementⁱ
- 26363795
viral process Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Glycosidase, Hydrolase
Biological process	Host-virus interaction

Enzyme and pathway databases

BioCycⁱ	MetaCyc:HS03036-MONOMER
BRENDAⁱ	3.2.1.35 2681

Protein family/group databases

Carbohydrate-Active enZymes More...CAZyⁱ	GH84 Glycoside Hydrolase Family 84

CAZyⁱ

GH84 Glycoside Hydrolase Family 84

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Protein O-GlcNAcase2 PublicationsCurated (EC:3.2.1.1691 Publication5 Publications) Short name: OGA1 Publication Alternative name(s): Beta-N-acetylglucosaminidase1 Publication Beta-N-acetylhexosaminidase Beta-hexosaminidase Meningioma-expressed antigen 51 Publication N-acetyl-beta-D-glucosaminidase N-acetyl-beta-glucosaminidase Nuclear cytoplasmic O-GlcNAcase and acetyltransferase Short name: NCOAT
Gene namesⁱ	Name:OGAImported Synonyms:HEXC, KIAA0679, MEA5, MGEA5
Organismⁱ	Homo sapiens (Human)
Taxonomic identifierⁱ	9606 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo
Proteomesⁱ	UP000005640 Componentⁱ: Chromosome 10

Organism-specific databases

EuPathDBⁱ	HostDB:ENSG00000198408.13
Human Gene Nomenclature Database More...HGNCⁱ	HGNC:7056 OGA
MIMⁱ	604039 gene
neXtProtⁱ	NX_O60502

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	69	Y → K or Q: Strongly reduces affinity for glycopeptide substrates. Nearly abolishes enzyme activity. 2 Publications	1
Mutagenesisⁱ	69	Y → S: Strongly reduces affinity for glycopeptide substrates. Nearly abolishes enzyme activity. 2 Publications	1
Mutagenesisⁱ	174	D → A: Nearly abolishes enzyme activity. 1 Publication	1
Mutagenesisⁱ	175	D → A: Nearly abolishes enzyme activity. 1 Publication	1
Mutagenesisⁱ	175	D → N: Nearly abolishes enzyme activity. 1 Publication	1
Mutagenesisⁱ	255	V → G or T: Nearly abolishes enzyme activity. 1 Publication	1
Mutagenesisⁱ	286	Y → S: Nearly abolishes enzyme activity. 1 Publication	1
Mutagenesisⁱ	287	D → A: Nearly abolishes enzyme activity. 1 Publication	1
Mutagenesisⁱ	413	D → A: Abrogates cleavage by caspase-3. 1 Publication	1
Mutagenesisⁱ	679	W → N: Nearly abolishes enzyme activity. 1 Publication	1

Organism-specific databases

DisGeNET More...DisGeNETⁱ	10724
Open Targets More...OpenTargetsⁱ	ENSG00000198408
PharmGKBⁱ	PA30787

Chemistry databases

ChEMBLⁱ	CHEMBL5921

ChEMBLⁱ

CHEMBL5921

Polymorphism and mutation databases

BioMutaⁱ	MGEA5

BioMutaⁱ

MGEA5

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000252118	1 – 916	Protein O-GlcNAcaseAdd BLAST		916

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	364	PhosphoserineCombined sources		1

Post-translational modificationⁱ

Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity.2 Publications

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	413 – 414	Cleavage; by caspase-31 Publication		2

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	O60502
jPOSTⁱ	O60502
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	O60502
PaxDbⁱ	O60502
PeptideAtlas More...PeptideAtlasⁱ	O60502
PRIDEⁱ	O60502
ProteomicsDBⁱ	49438 49439 [O60502-2] 49440 [O60502-3]

PTM databases

iPTMnetⁱ	O60502
PhosphoSitePlusⁱ	O60502

Miscellaneous databases

PMAP-CutDBⁱ	O60502

PMAP-CutDBⁱ

O60502

Expressionⁱ

Tissue specificityⁱ

Ubiquitous. Shows highest expression in the brain, placenta and pancreas.2 Publications

Gene expression databases

Bgeeⁱ	ENSG00000198408 Expressed in 233 organ(s), highest expression level in forebrain
CleanExⁱ	HS_MGEA5
ExpressionAtlasⁱ	O60502 baseline and differential
Genevisibleⁱ	O60502 HS

Organism-specific databases

Human Protein Atlas More...HPAⁱ	HPA036141 HPA076501

HPAⁱ

HPA036141
HPA076501

Interactionⁱ

Subunit structureⁱ

Monomer (PubMed:11788610). Interacts with CLOCK (By similarity).By similarity1 Publication

(Microbial infection) Interacts with human T-cell leukemia virus 1/HTLV-1 protein Tax; this interaction increases Tax interacting partner CREB1 O-GlcNAcylation.1 Publication

Protein-protein interaction databases

BioGridⁱ	115948, 38 interactors
IntActⁱ	O60502, 23 interactors
Molecular INTeraction database More...MINTⁱ	O60502
STRINGⁱ	9606.ENSP00000354850

Chemistry databases

BindingDBⁱ

O60502

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Turnⁱ	54 – 57	Combined sources	4
Beta strandⁱ	61 – 66	Combined sources	6
Beta strandⁱ	69 – 71	Combined sources	3
Helixⁱ	75 – 87	Combined sources	13
Beta strandⁱ	92 – 95	Combined sources	4
Helixⁱ	101 – 103	Combined sources	3
Turnⁱ	104 – 108	Combined sources	5
Helixⁱ	113 – 128	Combined sources	16
Beta strandⁱ	132 – 137	Combined sources	6
Turnⁱ	140 – 142	Combined sources	3
Helixⁱ	148 – 162	Combined sources	15
Turnⁱ	163 – 165	Combined sources	3
Beta strandⁱ	168 – 172	Combined sources	5
Helixⁱ	182 – 187	Combined sources	6
Beta strandⁱ	188 – 190	Combined sources	3
Helixⁱ	191 – 205	Combined sources	15
Beta strandⁱ	212 – 215	Combined sources	4
Helixⁱ	221 – 223	Combined sources	3
Beta strandⁱ	224 – 226	Combined sources	3
Turnⁱ	228 – 230	Combined sources	3
Helixⁱ	232 – 240	Combined sources	9
Beta strandⁱ	245 – 249	Combined sources	5
Beta strandⁱ	252 – 255	Combined sources	4
Helixⁱ	261 – 271	Combined sources	11
Beta strandⁱ	276 – 279	Combined sources	4
Helixⁱ	285 – 287	Combined sources	3
Helixⁱ	301 – 306	Combined sources	6
Beta strandⁱ	308 – 312	Combined sources	5
Helixⁱ	318 – 321	Combined sources	4
Helixⁱ	322 – 332	Combined sources	11
Turnⁱ	334 – 337	Combined sources	4
Helixⁱ	376 – 388	Combined sources	13
Turnⁱ	389 – 391	Combined sources	3
Beta strandⁱ	542 – 544	Combined sources	3
Helixⁱ	555 – 564	Combined sources	10
Helixⁱ	573 – 587	Combined sources	15
Helixⁱ	589 – 591	Combined sources	3
Helixⁱ	605 – 628	Combined sources	24
Helixⁱ	634 – 661	Combined sources	28
Turnⁱ	662 – 666	Combined sources	5
Turnⁱ	680 – 683	Combined sources	4
Helixⁱ	684 – 692	Combined sources	9
Turnⁱ	694 – 699	Combined sources	6
Turnⁱ	710 – 712	Combined sources	3

Show more details

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2YDQ	X-ray	2.60	T	402-408	[»]
	5M7R	X-ray	2.35	A/B	1-916	[»]
	5M7S	X-ray	2.40	A/B	1-916	[»]
	5M7T	X-ray	2.60	A/B	1-916	[»]
	5M7U	X-ray	2.30	A/B	1-916	[»]
	5TKE	X-ray	2.48	A/B	60-400	[»]
				A/B	553-704	[»]
	5UHK	X-ray	2.97	A/C	56-400	[»]
				B/D	544-705	[»]
	5UHL	X-ray	3.14	A/C	56-400	[»]
				B/D	544-705	[»]
	5UHO	X-ray	3.21	A/C	56-400	[»]
				B/D	544-705	[»]
	5UHP	X-ray	2.79	A/B/C/D	14-400	[»]
				E/F/G/H	554-705	[»]
	5UN8	X-ray	2.13	A/B/C/D	60-400	[»]
				A/B/C/D	553-704	[»]
	5UN9	X-ray	2.50	A/B	60-400	[»]
				A/B	553-704	[»]
	5VVO	X-ray	2.60	A/B	60-400	[»]
				A/B	553-704	[»]
	5VVT	X-ray	2.80	A/C	60-400	[»]
				A/C	553-704	[»]
	5VVU	X-ray	2.70	A/C	60-400	[»]
				A/C	553-704	[»]
	5VVV	X-ray	2.80	A/C	60-400	[»]
				A/C	553-704	[»]
	5VVX	X-ray	2.90	A/C	60-400	[»]
				A/C	553-704	[»]
ProteinModelPortalⁱ	O60502
SMRⁱ	O60502
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	278 – 280	Substrate bindingBy similarity		3

Sequence similaritiesⁱ

Belongs to the glycosyl hydrolase 84 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG3698 Eukaryota ENOG410XPBQ LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000007726
HOVERGENⁱ	HBG053044
InParanoidⁱ	O60502
KEGG Orthology (KO) More...KOⁱ	K15719
OMAⁱ	QFGCEAY
Database of Orthologous Groups More...OrthoDBⁱ	969618at2759
PhylomeDBⁱ	O60502
TreeFamⁱ	TF313732

Family and domain databases

InterProⁱ	View protein in InterPro IPR016181 Acyl_CoA_acyltransferase IPR011496 Beta-N-acetylglucosaminidase IPR017853 Glycoside_hydrolase_SF
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07555 NAGidase, 1 hit
SUPFAMⁱ	SSF51445 SSF51445, 1 hit SSF55729 SSF55729, 1 hit

Sequences (4+)ⁱ

Sequence statusⁱ: Complete.

This entry describes 4 isoformsⁱ produced by alternative splicing. Align Add to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show all Align All

Isoform 1 (identifier: O60502-1) [UniParc]FASTA Add to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVQKESQATL EERESELSSN PAASAGASLE PPAAPAPGED NPAGAGGAAV 
        60         70         80         90        100
AGAAGGARRF LCGVVEGFYG RPWVMEQRKE LFRRLQKWEL NTYLYAPKDD 
       110        120        130        140        150
YKHRMFWREM YSVEEAEQLM TLISAAREYE IEFIYAISPG LDITFSNPKE 
       160        170        180        190        200
VSTLKRKLDQ VSQFGCRSFA LLFDDIDHNM CAADKEVFSS FAHAQVSITN 
       210        220        230        240        250
EIYQYLGEPE TFLFCPTEYC GTFCYPNVSQ SPYLRTVGEK LLPGIEVLWT 
       260        270        280        290        300
GPKVVSKEIP VESIEEVSKI IKRAPVIWDN IHANDYDQKR LFLGPYKGRS 
       310        320        330        340        350
TELIPRLKGV LTNPNCEFEA NYVAIHTLAT WYKSNMNGVR KDVVMTDSED 
       360        370        380        390        400
STVSIQIKLE NEGSDEDIET DVLYSPQMAL KLALTEWLQE FGVPHQYSSR 
       410        420        430        440        450
QVAHSGAKAS VVDGTPLVAA PSLNATTVVT TVYQEPIMSQ GAALSGEPTT 
       460        470        480        490        500
LTKEEEKKQP DEEPMDMVVE KQEETDHKND NQILSEIVEA KMAEELKPMD 
       510        520        530        540        550
TDKESIAESK SPEMSMQEDC ISDIAPMQTD EQTNKEQFVP GPNEKPLYTA 
       560        570        580        590        600
EPVTLEDLQL LADLFYLPYE HGPKGAQMLR EFQWLRANSS VVSVNCKGKD 
       610        620        630        640        650
SEKIEEWRSR AAKFEEMCGL VMGMFTRLSN CANRTILYDM YSYVWDIKSI 
       660        670        680        690        700
MSMVKSFVQW LGCRSHSSAQ FLIGDQEPWA FRGGLAGEFQ RLLPIDGAND 
       710        720        730        740        750
LFFQPPPLTP TSKVYTIRPY FPKDEASVYK ICREMYDDGV GLPFQSQPDL 
       760        770        780        790        800
IGDKLVGGLL SLSLDYCFVL EDEDGICGYA LGTVDVTPFI KKCKISWIPF 
       810        820        830        840        850
MQEKYTKPNG DKELSEAEKI MLSFHEEQEV LPETFLANFP SLIKMDIHKK 
       860        870        880        890        900
VTDPSVAKSM MACLLSSLKA NGSRGAFCEV RPDDKRILEF YSKLGCFEIA 
       910 
KMEGFPKDVV ILGRSL

Length:916

Mass (Da):102,915

Last modified:March 1, 2001 - v2

Checksum:ⁱ01F8A64A9B1475C6

GO

Isoform 2 (identifier: O60502-2) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
346-398: Missing.
691-704: Missing.

« Hide

        10         20         30         40         50
MVQKESQATL EERESELSSN PAASAGASLE PPAAPAPGED NPAGAGGAAV 
        60         70         80         90        100
AGAAGGARRF LCGVVEGFYG RPWVMEQRKE LFRRLQKWEL NTYLYAPKDD 
       110        120        130        140        150
YKHRMFWREM YSVEEAEQLM TLISAAREYE IEFIYAISPG LDITFSNPKE 
       160        170        180        190        200
VSTLKRKLDQ VSQFGCRSFA LLFDDIDHNM CAADKEVFSS FAHAQVSITN 
       210        220        230        240        250
EIYQYLGEPE TFLFCPTEYC GTFCYPNVSQ SPYLRTVGEK LLPGIEVLWT 
       260        270        280        290        300
GPKVVSKEIP VESIEEVSKI IKRAPVIWDN IHANDYDQKR LFLGPYKGRS 
       310        320        330        340        350
TELIPRLKGV LTNPNCEFEA NYVAIHTLAT WYKSNMNGVR KDVVMSRQVA 
       360        370        380        390        400
HSGAKASVVD GTPLVAAPSL NATTVVTTVY QEPIMSQGAA LSGEPTTLTK 
       410        420        430        440        450
EEEKKQPDEE PMDMVVEKQE ETDHKNDNQI LSEIVEAKMA EELKPMDTDK 
       460        470        480        490        500
ESIAESKSPE MSMQEDCISD IAPMQTDEQT NKEQFVPGPN EKPLYTAEPV 
       510        520        530        540        550
TLEDLQLLAD LFYLPYEHGP KGAQMLREFQ WLRANSSVVS VNCKGKDSEK 
       560        570        580        590        600
IEEWRSRAAK FEEMCGLVMG MFTRLSNCAN RTILYDMYSY VWDIKSIMSM 
       610        620        630        640        650
VKSFVQWLGC RSHSSAQFLI GDQEPWAFRG GLAGEFQPPP LTPTSKVYTI 
       660        670        680        690        700
RPYFPKDEAS VYKICREMYD DGVGLPFQSQ PDLIGDKLVG GLLSLSLDYC 
       710        720        730        740        750
FVLEDEDGIC GYALGTVDVT PFIKKCKISW IPFMQEKYTK PNGDKELSEA 
       760        770        780        790        800
EKIMLSFHEE QEVLPETFLA NFPSLIKMDI HKKVTDPSVA KSMMACLLSS 
       810        820        830        840 
LKANGSRGAF CEVRPDDKRI LEFYSKLGCF EIAKMEGFPK DVVILGRSL

Show »

Length:849

Mass (Da):95,331

Checksum:ⁱA65EFD64BE0AD17C

GO

Isoform 3 (identifier: O60502-3) [UniParc]FASTA Add to basket

Also known as: MGEA5s

The sequence of this isoform differs from the canonical sequence as follows:
663-677: CRSHSSAQFLIGDQE → RCTRNNLFSSNILSL
678-916: Missing.

« Hide

        10         20         30         40         50
MVQKESQATL EERESELSSN PAASAGASLE PPAAPAPGED NPAGAGGAAV 
        60         70         80         90        100
AGAAGGARRF LCGVVEGFYG RPWVMEQRKE LFRRLQKWEL NTYLYAPKDD 
       110        120        130        140        150
YKHRMFWREM YSVEEAEQLM TLISAAREYE IEFIYAISPG LDITFSNPKE 
       160        170        180        190        200
VSTLKRKLDQ VSQFGCRSFA LLFDDIDHNM CAADKEVFSS FAHAQVSITN 
       210        220        230        240        250
EIYQYLGEPE TFLFCPTEYC GTFCYPNVSQ SPYLRTVGEK LLPGIEVLWT 
       260        270        280        290        300
GPKVVSKEIP VESIEEVSKI IKRAPVIWDN IHANDYDQKR LFLGPYKGRS 
       310        320        330        340        350
TELIPRLKGV LTNPNCEFEA NYVAIHTLAT WYKSNMNGVR KDVVMTDSED 
       360        370        380        390        400
STVSIQIKLE NEGSDEDIET DVLYSPQMAL KLALTEWLQE FGVPHQYSSR 
       410        420        430        440        450
QVAHSGAKAS VVDGTPLVAA PSLNATTVVT TVYQEPIMSQ GAALSGEPTT 
       460        470        480        490        500
LTKEEEKKQP DEEPMDMVVE KQEETDHKND NQILSEIVEA KMAEELKPMD 
       510        520        530        540        550
TDKESIAESK SPEMSMQEDC ISDIAPMQTD EQTNKEQFVP GPNEKPLYTA 
       560        570        580        590        600
EPVTLEDLQL LADLFYLPYE HGPKGAQMLR EFQWLRANSS VVSVNCKGKD 
       610        620        630        640        650
SEKIEEWRSR AAKFEEMCGL VMGMFTRLSN CANRTILYDM YSYVWDIKSI 
       660        670 
MSMVKSFVQW LGRCTRNNLF SSNILSL

Show »

Length:677

Mass (Da):76,193

Checksum:ⁱBF83DEF3ABDE4AA8

GO

Isoform 4 (identifier: O60502-4) [UniParc]FASTA Add to basket

The sequence of this isoform differs from the canonical sequence as follows:
346-398: Missing.

« Hide

        10         20         30         40         50
MVQKESQATL EERESELSSN PAASAGASLE PPAAPAPGED NPAGAGGAAV 
        60         70         80         90        100
AGAAGGARRF LCGVVEGFYG RPWVMEQRKE LFRRLQKWEL NTYLYAPKDD 
       110        120        130        140        150
YKHRMFWREM YSVEEAEQLM TLISAAREYE IEFIYAISPG LDITFSNPKE 
       160        170        180        190        200
VSTLKRKLDQ VSQFGCRSFA LLFDDIDHNM CAADKEVFSS FAHAQVSITN 
       210        220        230        240        250
EIYQYLGEPE TFLFCPTEYC GTFCYPNVSQ SPYLRTVGEK LLPGIEVLWT 
       260        270        280        290        300
GPKVVSKEIP VESIEEVSKI IKRAPVIWDN IHANDYDQKR LFLGPYKGRS 
       310        320        330        340        350
TELIPRLKGV LTNPNCEFEA NYVAIHTLAT WYKSNMNGVR KDVVMSRQVA 
       360        370        380        390        400
HSGAKASVVD GTPLVAAPSL NATTVVTTVY QEPIMSQGAA LSGEPTTLTK 
       410        420        430        440        450
EEEKKQPDEE PMDMVVEKQE ETDHKNDNQI LSEIVEAKMA EELKPMDTDK 
       460        470        480        490        500
ESIAESKSPE MSMQEDCISD IAPMQTDEQT NKEQFVPGPN EKPLYTAEPV 
       510        520        530        540        550
TLEDLQLLAD LFYLPYEHGP KGAQMLREFQ WLRANSSVVS VNCKGKDSEK 
       560        570        580        590        600
IEEWRSRAAK FEEMCGLVMG MFTRLSNCAN RTILYDMYSY VWDIKSIMSM 
       610        620        630        640        650
VKSFVQWLGC RSHSSAQFLI GDQEPWAFRG GLAGEFQRLL PIDGANDLFF 
       660        670        680        690        700
QPPPLTPTSK VYTIRPYFPK DEASVYKICR EMYDDGVGLP FQSQPDLIGD 
       710        720        730        740        750
KLVGGLLSLS LDYCFVLEDE DGICGYALGT VDVTPFIKKC KISWIPFMQE 
       760        770        780        790        800
KYTKPNGDKE LSEAEKIMLS FHEEQEVLPE TFLANFPSLI KMDIHKKVTD 
       810        820        830        840        850
PSVAKSMMAC LLSSLKANGS RGAFCEVRPD DKRILEFYSK LGCFEIAKME 
       860 
GFPKDVVILG RSL

Show »

Length:863

Mass (Da):96,932

Checksum:ⁱD6AB77EE6830F570

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	H7C3X0	H7C3X0_HUMAN		Protein O-GlcNAcase Protein O-GlcNAcase	OGA	256	Annotation score:

Sequence cautionⁱ

The sequence AAH47877 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

The sequence BAA31654 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱVAR_027761	46	G → E. Corresponds to variant dbSNP:rs3740421Ensembl.		1
Natural variantⁱVAR_027762	602	E → K1 PublicationCorresponds to variant dbSNP:rs17853930Ensembl.		1

Alternative sequence

Feature key	Position(s)	DescriptionActions	Length
Alternative sequenceⁱVSP_020866	346 – 398	Missing in isoform 2 and isoform 4. CuratedAdd BLAST	53
Alternative sequenceⁱVSP_020867	663 – 677	CRSHS…IGDQE → RCTRNNLFSSNILSL in isoform 3. 1 PublicationAdd BLAST	15
Alternative sequenceⁱVSP_020868	678 – 916	Missing in isoform 3. 1 PublicationAdd BLAST	239
Alternative sequenceⁱVSP_020869	691 – 704	Missing in isoform 2. CuratedAdd BLAST	14

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF036144 mRNA Translation: AAD05385.2 AF307332 mRNA Translation: AAG21428.1 AB014579 mRNA Translation: BAA31654.2 Different initiation. AC010789 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW49744.1 CH471066 Genomic DNA Translation: EAW49741.1 CH471066 Genomic DNA Translation: EAW49742.1 BC001343 mRNA Translation: AAH01343.1 BC039583 mRNA Translation: AAH39583.2 BC047877 mRNA Translation: AAH47877.1 Sequence problems.
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS44471.1 [O60502-4] CCDS7520.1 [O60502-1]
PIRⁱ	T00360
NCBI Reference Sequences More...RefSeqⁱ	NP_001135906.1, NM_001142434.1 [O60502-4] NP_036347.1, NM_012215.3 [O60502-1]
UniGeneⁱ	Hs.500842

Genome annotation databases

Ensemblⁱ	ENST00000357797; ENSP00000350445; ENSG00000198408 [O60502-2] ENST00000361464; ENSP00000354850; ENSG00000198408 [O60502-1] ENST00000370094; ENSP00000359112; ENSG00000198408 [O60502-3] ENST00000439817; ENSP00000409973; ENSG00000198408 [O60502-4]
GeneIDⁱ	10724
KEGGⁱ	hsa:10724
UCSC genome browser More...UCSCⁱ	uc001ktv.3 human [O60502-1]

Keywords - Coding sequence diversityⁱ

Alternative splicing, Polymorphism

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
O60502	O-GlcNAcase		PANPA		916	UniRef100_O60502
O-GlcNAcase		GORGO		916
cDNA FLJ11229 fis, clone PLACE1008356, highly similar to Homo sapiens meningioma expressed antigen 5 ((MGEA5), mRNA)		HUMAN		479
UPI000C2B4C15		Ugandan red Colobus		1108

Protein	Similar proteins		Species	Score	Length	Source
O60502	O-GlcNAcase		PANPA		916	UniRef90_O60502
O-GlcNAcase		GORGO		916
cDNA FLJ11229 fis, clone PLACE1008356, highly similar to Homo sapiens meningioma expressed antigen 5 ((MGEA5), mRNA)		HUMAN		479
O-GlcNAcase		MACMU		1107
O-GlcNAcase		CALJA		1107
+175
O60502-2	protein O-GlcNAcase isoform X3		ERIEU		849	UniRef90_O60502-2
MGEA5 isoform 4		PONAB		849
MGEA5 isoform 2		PANTR		849
UPI00045DE7D6		Orycteropus afer afer		852
UPI00020AA56B		NOMLE		849
O60502-3	O-GlcNAcase		COLAP		805	UniRef90_O60502-3
Meningioma expressed antigen 5 (Fragment)		MUSPF		701
Putative meningioma-expressed antigen 5s splice variant meningioma		DESRO		696
O-GlcNAcase		RHIRO		691
O-GlcNAcase		PANTR		687
+9
O60502-4	O-GlcNAcase		MACMU		873	UniRef90_O60502-4
O-GlcNAcase		MACNE		873
O-GlcNAcase		RHIBE		872
O-GlcNAcase		PANTR		871
Bifunctional protein NCOAT isoform b		CALJA		871
+41

Protein	Similar proteins		Species	Score	Length	Source
O60502	O-GlcNAcase		PANPA		916	UniRef50_O60502
O-GlcNAcase		GORGO		916
cDNA FLJ11229 fis, clone PLACE1008356, highly similar to Homo sapiens meningioma expressed antigen 5 ((MGEA5), mRNA)		HUMAN		479
O-GlcNAcase		MACMU		1107
O-GlcNAcase		CALJA		1107
+438
O60502-2	MGEA5 isoform 4		PONAB		849	UniRef50_O60502-2
protein O-GlcNAcase isoform X3		ERIEU		849
MGEA5 isoform 2		PANTR		849
O-GlcNAcase		MELGA		599
Uncharacterized protein		Amazon coral snake		701
+12
O60502-3	O-GlcNAcase		COLAP		805	UniRef50_O60502-3
Meningioma expressed antigen 5 (Fragment)		MUSPF		701
Putative meningioma-expressed antigen 5s splice variant meningioma		DESRO		696
O-GlcNAcase		RHIRO		691
O-GlcNAcase		PANTR		687
+21
O60502-4	O-GlcNAcase		MACMU		873	UniRef50_O60502-4
O-GlcNAcase		MACNE		873
O-GlcNAcase		RHIBE		872
O-GlcNAcase		PANTR		871
O-GlcNAcase		PANPA		871
+55

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF036144 mRNA Translation: AAD05385.2 AF307332 mRNA Translation: AAG21428.1 AB014579 mRNA Translation: BAA31654.2 Different initiation. AC010789 Genomic DNA No translation available. CH471066 Genomic DNA Translation: EAW49744.1 CH471066 Genomic DNA Translation: EAW49741.1 CH471066 Genomic DNA Translation: EAW49742.1 BC001343 mRNA Translation: AAH01343.1 BC039583 mRNA Translation: AAH39583.2 BC047877 mRNA Translation: AAH47877.1 Sequence problems.
CCDSⁱ	CCDS44471.1 [O60502-4] CCDS7520.1 [O60502-1]
PIRⁱ	T00360
RefSeqⁱ	NP_001135906.1, NM_001142434.1 [O60502-4] NP_036347.1, NM_012215.3 [O60502-1]
UniGeneⁱ	Hs.500842

3D structure databases

Select the link destinations: PDBeⁱ RCSB PDBⁱ PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	2YDQ	X-ray	2.60	T	402-408	[»]
	5M7R	X-ray	2.35	A/B	1-916	[»]
	5M7S	X-ray	2.40	A/B	1-916	[»]
	5M7T	X-ray	2.60	A/B	1-916	[»]
	5M7U	X-ray	2.30	A/B	1-916	[»]
	5TKE	X-ray	2.48	A/B	60-400	[»]
				A/B	553-704	[»]
	5UHK	X-ray	2.97	A/C	56-400	[»]
				B/D	544-705	[»]
	5UHL	X-ray	3.14	A/C	56-400	[»]
				B/D	544-705	[»]
	5UHO	X-ray	3.21	A/C	56-400	[»]
				B/D	544-705	[»]
	5UHP	X-ray	2.79	A/B/C/D	14-400	[»]
				E/F/G/H	554-705	[»]
	5UN8	X-ray	2.13	A/B/C/D	60-400	[»]
				A/B/C/D	553-704	[»]
	5UN9	X-ray	2.50	A/B	60-400	[»]
				A/B	553-704	[»]
	5VVO	X-ray	2.60	A/B	60-400	[»]
				A/B	553-704	[»]
	5VVT	X-ray	2.80	A/C	60-400	[»]
				A/C	553-704	[»]
	5VVU	X-ray	2.70	A/C	60-400	[»]
				A/C	553-704	[»]
	5VVV	X-ray	2.80	A/C	60-400	[»]
				A/C	553-704	[»]
	5VVX	X-ray	2.90	A/C	60-400	[»]
				A/C	553-704	[»]
ProteinModelPortalⁱ	O60502
SMRⁱ	O60502
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	115948, 38 interactors
IntActⁱ	O60502, 23 interactors
MINTⁱ	O60502
STRINGⁱ	9606.ENSP00000354850

Chemistry databases

BindingDBⁱ	O60502
ChEMBLⁱ	CHEMBL5921

Protein family/group databases

CAZyⁱ	GH84 Glycoside Hydrolase Family 84

CAZyⁱ

GH84 Glycoside Hydrolase Family 84

PTM databases

iPTMnetⁱ	O60502
PhosphoSitePlusⁱ	O60502

Polymorphism and mutation databases

BioMutaⁱ	MGEA5

BioMutaⁱ

MGEA5

Proteomic databases

EPDⁱ	O60502
jPOSTⁱ	O60502
MaxQBⁱ	O60502
PaxDbⁱ	O60502
PeptideAtlasⁱ	O60502
PRIDEⁱ	O60502
ProteomicsDBⁱ	49438 49439 [O60502-2] 49440 [O60502-3]

Protocols and materials databases

The DNASU plasmid repository More...DNASUⁱ	10724
Structural Biology Knowledgebase	Search...

Genome annotation databases

Ensemblⁱ	ENST00000357797; ENSP00000350445; ENSG00000198408 [O60502-2] ENST00000361464; ENSP00000354850; ENSG00000198408 [O60502-1] ENST00000370094; ENSP00000359112; ENSG00000198408 [O60502-3] ENST00000439817; ENSP00000409973; ENSG00000198408 [O60502-4]
GeneIDⁱ	10724
KEGGⁱ	hsa:10724
UCSCⁱ	uc001ktv.3 human [O60502-1]

Organism-specific databases

CTDⁱ	10724
DisGeNETⁱ	10724
EuPathDBⁱ	HostDB:ENSG00000198408.13
GeneCardsⁱ	OGA
HGNCⁱ	HGNC:7056 OGA
HPAⁱ	HPA036141 HPA076501
MIMⁱ	604039 gene
neXtProtⁱ	NX_O60502
OpenTargetsⁱ	ENSG00000198408
PharmGKBⁱ	PA30787
HUGEⁱ	Search...
GenAtlas: human gene database More...GenAtlasⁱ	Search...

Phylogenomic databases

eggNOGⁱ	KOG3698 Eukaryota ENOG410XPBQ LUCA
GeneTreeⁱ	ENSGT00390000007726
HOVERGENⁱ	HBG053044
InParanoidⁱ	O60502
KOⁱ	K15719
OMAⁱ	QFGCEAY
OrthoDBⁱ	969618at2759
PhylomeDBⁱ	O60502
TreeFamⁱ	TF313732

Enzyme and pathway databases

BioCycⁱ	MetaCyc:HS03036-MONOMER
BRENDAⁱ	3.2.1.35 2681

Miscellaneous databases

ChiTaRSⁱ	MGEA5 human
GeneWikiⁱ	MGEA5
GenomeRNAiⁱ	10724
PMAP-CutDBⁱ	O60502
Protein Ontology More...PROⁱ	PR:O60502
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSG00000198408 Expressed in 233 organ(s), highest expression level in forebrain
CleanExⁱ	HS_MGEA5
ExpressionAtlasⁱ	O60502 baseline and differential
Genevisibleⁱ	O60502 HS

Family and domain databases

InterProⁱ	View protein in InterPro IPR016181 Acyl_CoA_acyltransferase IPR011496 Beta-N-acetylglucosaminidase IPR017853 Glycoside_hydrolase_SF
Pfamⁱ	View protein in Pfam PF07555 NAGidase, 1 hit
SUPFAMⁱ	SSF51445 SSF51445, 1 hit SSF55729 SSF55729, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	OGA_HUMAN
Accessionⁱ	O60502Primary (citable) accession number: O60502 Secondary accession number(s): B7WPB9 Q9HAR0
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 3, 2006
	Last sequence update:	March 1, 2001
	Last modified:	January 16, 2019
	This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program
Disclaimer	Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Reference proteome

Documents

Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries
SIMILARITY comments
Index of protein domains and families
Human entries with polymorphisms or disease mutations
List of human entries with polymorphisms or disease mutations
Human polymorphisms and disease mutations
Index of human polymorphisms and disease mutations
MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
Human chromosome 10
Human chromosome 10: entries, gene names and cross-references to MIM

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UniProtKB - O60502 (OGA_HUMAN)

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Protein O-GlcNAcase

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Caution

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

pH dependencei

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<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

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Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

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Organism-specific databases

Chemistry databases

Polymorphism and mutation databases

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Sites

Keywords - PTMi

Proteomic databases

PTM databases

Miscellaneous databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

Organism-specific databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

Chemistry databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Natural variant

Alternative sequence

Sequence databases

Genome annotation databases

Keywords - Coding sequence diversityi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Protein family/group databases

PTM databases

Polymorphism and mutation databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

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Functionⁱ

Catalytic activityⁱ

Kineticsⁱ

pH dependenceⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Keywords - Coding sequence diversityⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ