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Protein

Protein O-GlcNAcase

Gene

OGA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).5 Publications
Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.1 Publication

Caution

The mouse and rat orthologs were initially identified as bi-functional proteins containing an N-terminal domain with O-GlcNAcase activity and a C-terminal domain with histone acetyltransferase activity. The histone acetyltransferase activity was detected only when the protein was expressed in mammalian cells, but not when expressed in bacterial cells, suggesting that the histone acetyltransferase activity might be due to the presence of a contaminant. Comparison of the human protein with a bacterial putative acetyltransferase (AC Q2CEE2) shows that the residues important for acetyl-CoA binding are not conserved, and that the residues proposed to be important for histone acetyltransferase activity are not in a position where they could participate in catalysis. Characterization of the human protein shows that it does not bind acetyl-CoA and therefore cannot have acetyltransferase activity.1 Publication1 Publication

Catalytic activityi

[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H2O = [protein]-L-serine + N-acetyl-D-glucosamine.1 Publication5 Publications
[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-threonine + H2O = [protein]-L-threonine + N-acetyl-D-glucosamine.1 Publication5 Publications

Activity regulationi

Inhibited by N-acetylglucosamine and not N-acetylgalactosamine.1 Publication

Kineticsi

  1. KM=1.1 mM for pNP-GlcNAc1 Publication
  1. Vmax=652 µmol/min/mg enzyme with pNP-GLcNAc as substrate1 Publication

pH dependencei

Optimum pH is 5.7-7.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei67Substrate; via carbonyl oxygenBy similarity1
Binding sitei98SubstrateBy similarity1
Binding sitei174SubstrateBy similarity1
Active sitei175Proton donor1 Publication1
Binding sitei219SubstrateBy similarity1
Binding sitei285SubstrateBy similarity1
Binding sitei313SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • glycoprotein catabolic process Source: ProtInc
  • glycoprotein metabolic process Source: GO_Central
  • N-acetylglucosamine metabolic process Source: UniProtKB
  • protein deglycosylation Source: UniProtKB
  • protein O-linked glycosylation Source: ParkinsonsUK-UCL
  • viral process Source: UniProtKB-KW

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processHost-virus interaction

Enzyme and pathway databases

BioCyciMetaCyc:HS03036-MONOMER
BRENDAi3.2.1.35 2681

Protein family/group databases

CAZyiGH84 Glycoside Hydrolase Family 84

Names & Taxonomyi

Protein namesi
Recommended name:
Protein O-GlcNAcase2 PublicationsCurated (EC:3.2.1.1691 Publication5 Publications)
Short name:
OGA1 Publication
Alternative name(s):
Beta-N-acetylglucosaminidase1 Publication
Beta-N-acetylhexosaminidase
Beta-hexosaminidase
Meningioma-expressed antigen 51 Publication
N-acetyl-beta-D-glucosaminidase
N-acetyl-beta-glucosaminidase
Nuclear cytoplasmic O-GlcNAcase and acetyltransferase
Short name:
NCOAT
Gene namesi
Name:OGAImported
Synonyms:HEXC, KIAA0679, MEA5, MGEA5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000198408.13
HGNCiHGNC:7056 OGA
MIMi604039 gene
neXtProtiNX_O60502

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi69Y → K or Q: Strongly reduces affinity for glycopeptide substrates. Nearly abolishes enzyme activity. 2 Publications1
Mutagenesisi69Y → S: Strongly reduces affinity for glycopeptide substrates. Nearly abolishes enzyme activity. 2 Publications1
Mutagenesisi174D → A: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi175D → A: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi175D → N: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi255V → G or T: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi286Y → S: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi287D → A: Nearly abolishes enzyme activity. 1 Publication1
Mutagenesisi413D → A: Abrogates cleavage by caspase-3. 1 Publication1
Mutagenesisi679W → N: Nearly abolishes enzyme activity. 1 Publication1

Organism-specific databases

DisGeNETi10724
OpenTargetsiENSG00000198408
PharmGKBiPA30787

Chemistry databases

ChEMBLiCHEMBL5921

Polymorphism and mutation databases

BioMutaiMGEA5

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002521181 – 916Protein O-GlcNAcaseAdd BLAST916

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei364PhosphoserineCombined sources1

Post-translational modificationi

Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei413 – 414Cleavage; by caspase-31 Publication2

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO60502
MaxQBiO60502
PaxDbiO60502
PeptideAtlasiO60502
PRIDEiO60502
ProteomicsDBi49438
49439 [O60502-2]
49440 [O60502-3]

PTM databases

iPTMnetiO60502
PhosphoSitePlusiO60502

Miscellaneous databases

PMAP-CutDBiO60502

Expressioni

Tissue specificityi

Ubiquitous. Shows highest expression in the brain, placenta and pancreas.2 Publications

Gene expression databases

BgeeiENSG00000198408 Expressed in 233 organ(s), highest expression level in forebrain
CleanExiHS_MGEA5
ExpressionAtlasiO60502 baseline and differential
GenevisibleiO60502 HS

Organism-specific databases

HPAiHPA036141
HPA076501

Interactioni

Subunit structurei

Monomer (PubMed:11788610). Interacts with CLOCK (By similarity).By similarity1 Publication
(Microbial infection) Interacts with human T-cell leukemia virus 1/HTLV-1 protein Tax; this interaction increases Tax interacting partner CREB1 O-GlcNAcylation.1 Publication

Protein-protein interaction databases

BioGridi115948, 38 interactors
IntActiO60502, 23 interactors
MINTiO60502
STRINGi9606.ENSP00000354850

Chemistry databases

BindingDBiO60502

Structurei

Secondary structure

1916
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO60502
SMRiO60502
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni278 – 280Substrate bindingBy similarity3

Sequence similaritiesi

Belongs to the glycosyl hydrolase 84 family.Curated

Phylogenomic databases

eggNOGiKOG3698 Eukaryota
ENOG410XPBQ LUCA
GeneTreeiENSGT00390000007726
HOVERGENiHBG053044
InParanoidiO60502
KOiK15719
OMAiQFGCEAY
OrthoDBiEOG091G02T9
PhylomeDBiO60502
TreeFamiTF313732

Family and domain databases

InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR011496 Beta-N-acetylglucosaminidase
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF07555 NAGidase, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF55729 SSF55729, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O60502-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVQKESQATL EERESELSSN PAASAGASLE PPAAPAPGED NPAGAGGAAV
60 70 80 90 100
AGAAGGARRF LCGVVEGFYG RPWVMEQRKE LFRRLQKWEL NTYLYAPKDD
110 120 130 140 150
YKHRMFWREM YSVEEAEQLM TLISAAREYE IEFIYAISPG LDITFSNPKE
160 170 180 190 200
VSTLKRKLDQ VSQFGCRSFA LLFDDIDHNM CAADKEVFSS FAHAQVSITN
210 220 230 240 250
EIYQYLGEPE TFLFCPTEYC GTFCYPNVSQ SPYLRTVGEK LLPGIEVLWT
260 270 280 290 300
GPKVVSKEIP VESIEEVSKI IKRAPVIWDN IHANDYDQKR LFLGPYKGRS
310 320 330 340 350
TELIPRLKGV LTNPNCEFEA NYVAIHTLAT WYKSNMNGVR KDVVMTDSED
360 370 380 390 400
STVSIQIKLE NEGSDEDIET DVLYSPQMAL KLALTEWLQE FGVPHQYSSR
410 420 430 440 450
QVAHSGAKAS VVDGTPLVAA PSLNATTVVT TVYQEPIMSQ GAALSGEPTT
460 470 480 490 500
LTKEEEKKQP DEEPMDMVVE KQEETDHKND NQILSEIVEA KMAEELKPMD
510 520 530 540 550
TDKESIAESK SPEMSMQEDC ISDIAPMQTD EQTNKEQFVP GPNEKPLYTA
560 570 580 590 600
EPVTLEDLQL LADLFYLPYE HGPKGAQMLR EFQWLRANSS VVSVNCKGKD
610 620 630 640 650
SEKIEEWRSR AAKFEEMCGL VMGMFTRLSN CANRTILYDM YSYVWDIKSI
660 670 680 690 700
MSMVKSFVQW LGCRSHSSAQ FLIGDQEPWA FRGGLAGEFQ RLLPIDGAND
710 720 730 740 750
LFFQPPPLTP TSKVYTIRPY FPKDEASVYK ICREMYDDGV GLPFQSQPDL
760 770 780 790 800
IGDKLVGGLL SLSLDYCFVL EDEDGICGYA LGTVDVTPFI KKCKISWIPF
810 820 830 840 850
MQEKYTKPNG DKELSEAEKI MLSFHEEQEV LPETFLANFP SLIKMDIHKK
860 870 880 890 900
VTDPSVAKSM MACLLSSLKA NGSRGAFCEV RPDDKRILEF YSKLGCFEIA
910
KMEGFPKDVV ILGRSL
Length:916
Mass (Da):102,915
Last modified:March 1, 2001 - v2
Checksum:i01F8A64A9B1475C6
GO
Isoform 2 (identifier: O60502-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     346-398: Missing.
     691-704: Missing.

Show »
Length:849
Mass (Da):95,331
Checksum:iA65EFD64BE0AD17C
GO
Isoform 3 (identifier: O60502-3) [UniParc]FASTAAdd to basket
Also known as: MGEA5s

The sequence of this isoform differs from the canonical sequence as follows:
     663-677: CRSHSSAQFLIGDQE → RCTRNNLFSSNILSL
     678-916: Missing.

Show »
Length:677
Mass (Da):76,193
Checksum:iBF83DEF3ABDE4AA8
GO
Isoform 4 (identifier: O60502-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     346-398: Missing.

Show »
Length:863
Mass (Da):96,932
Checksum:iD6AB77EE6830F570
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H7C3X0H7C3X0_HUMAN
Protein O-GlcNAcase
OGA
256Annotation score:

Sequence cautioni

The sequence AAH47877 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAA31654 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_02776146G → E. Corresponds to variant dbSNP:rs3740421Ensembl.1
Natural variantiVAR_027762602E → K1 PublicationCorresponds to variant dbSNP:rs17853930Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_020866346 – 398Missing in isoform 2 and isoform 4. CuratedAdd BLAST53
Alternative sequenceiVSP_020867663 – 677CRSHS…IGDQE → RCTRNNLFSSNILSL in isoform 3. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_020868678 – 916Missing in isoform 3. 1 PublicationAdd BLAST239
Alternative sequenceiVSP_020869691 – 704Missing in isoform 2. CuratedAdd BLAST14

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036144 mRNA Translation: AAD05385.2
AF307332 mRNA Translation: AAG21428.1
AB014579 mRNA Translation: BAA31654.2 Different initiation.
AC010789 Genomic DNA No translation available.
CH471066 Genomic DNA Translation: EAW49744.1
CH471066 Genomic DNA Translation: EAW49741.1
CH471066 Genomic DNA Translation: EAW49742.1
BC001343 mRNA Translation: AAH01343.1
BC039583 mRNA Translation: AAH39583.2
BC047877 mRNA Translation: AAH47877.1 Sequence problems.
CCDSiCCDS44471.1 [O60502-4]
CCDS7520.1 [O60502-1]
PIRiT00360
RefSeqiNP_001135906.1, NM_001142434.1 [O60502-4]
NP_036347.1, NM_012215.3 [O60502-1]
UniGeneiHs.500842

Genome annotation databases

EnsembliENST00000357797; ENSP00000350445; ENSG00000198408 [O60502-2]
ENST00000361464; ENSP00000354850; ENSG00000198408 [O60502-1]
ENST00000370094; ENSP00000359112; ENSG00000198408 [O60502-3]
ENST00000439817; ENSP00000409973; ENSG00000198408 [O60502-4]
GeneIDi10724
KEGGihsa:10724
UCSCiuc001ktv.3 human [O60502-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF036144 mRNA Translation: AAD05385.2
AF307332 mRNA Translation: AAG21428.1
AB014579 mRNA Translation: BAA31654.2 Different initiation.
AC010789 Genomic DNA No translation available.
CH471066 Genomic DNA Translation: EAW49744.1
CH471066 Genomic DNA Translation: EAW49741.1
CH471066 Genomic DNA Translation: EAW49742.1
BC001343 mRNA Translation: AAH01343.1
BC039583 mRNA Translation: AAH39583.2
BC047877 mRNA Translation: AAH47877.1 Sequence problems.
CCDSiCCDS44471.1 [O60502-4]
CCDS7520.1 [O60502-1]
PIRiT00360
RefSeqiNP_001135906.1, NM_001142434.1 [O60502-4]
NP_036347.1, NM_012215.3 [O60502-1]
UniGeneiHs.500842

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YDQX-ray2.60T402-408[»]
5M7RX-ray2.35A/B1-916[»]
5M7SX-ray2.40A/B1-916[»]
5M7TX-ray2.60A/B1-916[»]
5M7UX-ray2.30A/B1-916[»]
5TKEX-ray2.48A/B60-400[»]
A/B553-704[»]
5UHKX-ray2.97A/C56-400[»]
B/D544-705[»]
5UHLX-ray3.14A/C56-400[»]
B/D544-705[»]
5UHOX-ray3.21A/C56-400[»]
B/D544-705[»]
5UHPX-ray2.79A/B/C/D14-400[»]
E/F/G/H554-705[»]
5UN8X-ray2.13A/B/C/D60-400[»]
A/B/C/D553-704[»]
5UN9X-ray2.50A/B60-400[»]
A/B553-704[»]
5VVOX-ray2.60A/B60-400[»]
A/B553-704[»]
5VVTX-ray2.80A/C60-400[»]
A/C553-704[»]
5VVUX-ray2.70A/C60-400[»]
A/C553-704[»]
5VVVX-ray2.80A/C60-400[»]
A/C553-704[»]
5VVXX-ray2.90A/C60-400[»]
A/C553-704[»]
ProteinModelPortaliO60502
SMRiO60502
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115948, 38 interactors
IntActiO60502, 23 interactors
MINTiO60502
STRINGi9606.ENSP00000354850

Chemistry databases

BindingDBiO60502
ChEMBLiCHEMBL5921

Protein family/group databases

CAZyiGH84 Glycoside Hydrolase Family 84

PTM databases

iPTMnetiO60502
PhosphoSitePlusiO60502

Polymorphism and mutation databases

BioMutaiMGEA5

Proteomic databases

EPDiO60502
MaxQBiO60502
PaxDbiO60502
PeptideAtlasiO60502
PRIDEiO60502
ProteomicsDBi49438
49439 [O60502-2]
49440 [O60502-3]

Protocols and materials databases

DNASUi10724
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000357797; ENSP00000350445; ENSG00000198408 [O60502-2]
ENST00000361464; ENSP00000354850; ENSG00000198408 [O60502-1]
ENST00000370094; ENSP00000359112; ENSG00000198408 [O60502-3]
ENST00000439817; ENSP00000409973; ENSG00000198408 [O60502-4]
GeneIDi10724
KEGGihsa:10724
UCSCiuc001ktv.3 human [O60502-1]

Organism-specific databases

CTDi10724
DisGeNETi10724
EuPathDBiHostDB:ENSG00000198408.13
GeneCardsiOGA
HGNCiHGNC:7056 OGA
HPAiHPA036141
HPA076501
MIMi604039 gene
neXtProtiNX_O60502
OpenTargetsiENSG00000198408
PharmGKBiPA30787
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3698 Eukaryota
ENOG410XPBQ LUCA
GeneTreeiENSGT00390000007726
HOVERGENiHBG053044
InParanoidiO60502
KOiK15719
OMAiQFGCEAY
OrthoDBiEOG091G02T9
PhylomeDBiO60502
TreeFamiTF313732

Enzyme and pathway databases

BioCyciMetaCyc:HS03036-MONOMER
BRENDAi3.2.1.35 2681

Miscellaneous databases

ChiTaRSiMGEA5 human
GeneWikiiMGEA5
GenomeRNAii10724
PMAP-CutDBiO60502
PROiPR:O60502
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000198408 Expressed in 233 organ(s), highest expression level in forebrain
CleanExiHS_MGEA5
ExpressionAtlasiO60502 baseline and differential
GenevisibleiO60502 HS

Family and domain databases

InterProiView protein in InterPro
IPR016181 Acyl_CoA_acyltransferase
IPR011496 Beta-N-acetylglucosaminidase
IPR017853 Glycoside_hydrolase_SF
PfamiView protein in Pfam
PF07555 NAGidase, 1 hit
SUPFAMiSSF51445 SSF51445, 1 hit
SSF55729 SSF55729, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOGA_HUMAN
AccessioniPrimary (citable) accession number: O60502
Secondary accession number(s): B7WPB9
, D3DR79, E9PGF9, O75166, Q86WV0, Q8IV98, Q9BVA5, Q9HAR0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: March 1, 2001
Last modified: November 7, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  7. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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