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UniProtKB - O60476 (MA1A2_HUMAN)
Protein
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB
Gene
MAN1A2
Organism
Homo sapiens (Human)
Status
Functioni
Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.
Catalytic activityi
- 4 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) = 4 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
- 3 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) = 3 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)By similarityEC:3.2.1.113By similarity
Cofactori
Ca2+By similarity
Activity regulationi
Inhibited by both 1-deoxymannojirimycin and kifunensine.
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarityView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 508 | Proton donorBy similarity | 1 | |
Metal bindingi | 619 | CalciumBy similarity | 1 |
GO - Molecular functioni
- calcium ion binding Source: InterPro
- mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: GO_Central
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- Golgi apparatus mannose trimming Source: Reactome
- lung alveolus development Source: Ensembl
- N-glycan processing Source: GO_Central
- protein glycosylation Source: UniProtKB-UniPathway
- respiratory gaseous exchange by respiratory system Source: Ensembl
Keywordsi
Molecular function | Glycosidase, Hydrolase |
Ligand | Calcium, Metal-binding |
Enzyme and pathway databases
BRENDAi | 3.2.1.113, 2681 |
PathwayCommonsi | O60476 |
Reactomei | R-HSA-6811438, Intra-Golgi traffic R-HSA-964827, Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2 |
SignaLinki | O60476 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
Names & Taxonomyi
Protein namesi | Recommended name: Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (EC:3.2.1.113By similarity)Alternative name(s): Mannosidase alpha class 1A member 2 Processing alpha-1,2-mannosidase IB Short name: Alpha-1,2-mannosidase IB |
Gene namesi | Name:MAN1A2 Synonyms:MAN1B |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:6822, MAN1A2 |
MIMi | 604345, gene |
neXtProti | NX_O60476 |
VEuPathDBi | HostDB:ENSG00000198162 |
Subcellular locationi
Golgi apparatus
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Extracellular region or secreted
- extracellular exosome Source: UniProtKB
Golgi apparatus
- Golgi apparatus Source: HPA
- Golgi membrane Source: GO_Central
Other locations
- integral component of membrane Source: ProtInc
- membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 36 | CytoplasmicSequence analysisAdd BLAST | 35 | |
Transmembranei | 37 – 57 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 58 – 641 | LumenalSequence analysisAdd BLAST | 584 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 10905 |
OpenTargetsi | ENSG00000198162 |
PharmGKBi | PA30571 |
Miscellaneous databases
Pharosi | O60476, Tbio |
Genetic variation databases
BioMutai | MAN1A2 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000210312 | 2 – 641 | Mannosyl-oligosaccharide 1,2-alpha-mannosidase IBAdd BLAST | 640 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylthreonineCombined sources | 1 | |
Disulfide bondi | 462 ↔ 494 | By similarity | ||
Glycosylationi | 631 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Acetylation, Disulfide bond, GlycoproteinProteomic databases
EPDi | O60476 |
jPOSTi | O60476 |
MassIVEi | O60476 |
MaxQBi | O60476 |
PaxDbi | O60476 |
PeptideAtlasi | O60476 |
PRIDEi | O60476 |
ProteomicsDBi | 49416 |
PTM databases
GlyGeni | O60476, 1 site |
iPTMneti | O60476 |
PhosphoSitePlusi | O60476 |
Expressioni
Tissue specificityi
Highest levels of expression in placenta and testis.
Gene expression databases
Bgeei | ENSG00000198162, Expressed in chorionic villus and 247 other tissues |
ExpressionAtlasi | O60476, baseline and differential |
Genevisiblei | O60476, HS |
Organism-specific databases
HPAi | ENSG00000198162, Low tissue specificity |
Interactioni
Protein-protein interaction databases
BioGRIDi | 116111, 34 interactors |
IntActi | O60476, 19 interactors |
MINTi | O60476 |
STRINGi | 9606.ENSP00000348959 |
Miscellaneous databases
RNActi | O60476, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 153 – 175 | DisorderedSequence analysisAdd BLAST | 23 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 47 family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2204, Eukaryota |
GeneTreei | ENSGT00940000157498 |
HOGENOMi | CLU_003818_3_2_1 |
InParanoidi | O60476 |
OMAi | YSYLIHA |
OrthoDBi | 693882at2759 |
PhylomeDBi | O60476 |
TreeFami | TF313420 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
O60476-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTPALLPLS GRRIPPLNLG PPSFPHHRAT LRLSEKFILL LILSAFITLC
60 70 80 90 100
FGAFFFLPDS SKHKRFDLGL EDVLIPHVDA GKGAKNPGVF LIHGPDEHRH
110 120 130 140 150
REEEERLRNK IRADHEKALE EAKEKLRKSR EEIRAEIQTE KNKVVQEMKI
160 170 180 190 200
KENKPLPPVP IPNLVGIRGG DPEDNDIREK REKIKEMMKH AWDNYRTYGW
210 220 230 240 250
GHNELRPIAR KGHSPNIFGS SQMGATIVDA LDTLYIMGLH DEFLDGQRWI
260 270 280 290 300
EDNLDFSVNS EVSVFEVNIR FIGGLLAAYY LSGEEIFKIK AVQLAEKLLP
310 320 330 340 350
AFNTPTGIPW AMVNLKSGVG RNWGWASAGS SILAEFGTLH MEFIHLSYLT
360 370 380 390 400
GDLTYYKKVM HIRKLLQKMD RPNGLYPNYL NPRTGRWGQY HTSVGGLGDS
410 420 430 440 450
FYEYLLKAWL MSDKTDHEAR KMYDDAIEAI EKHLIKKSRG GLTFIGEWKN
460 470 480 490 500
GHLEKKMGHL ACFAGGMFAL GADGSRADKA GHYLELGAEI ARTCHESYDR
510 520 530 540 550
TALKLGPESF KFDGAVEAVA VRQAEKYYIL RPEVIETYWY LWRFTHDPRY
560 570 580 590 600
RQWGWEAALA IEKYCRVNGG FSGVKDVYSS TPTHDDVQQS FFLAETLKYL
610 620 630 640
YLLFSGDDLL PLDHWVFNTE AHPLPVLHLA NTTLSGNPAV R
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketH0Y543 | H0Y543_HUMAN | alpha-1,2-Mannosidase | MAN1A2 | 204 | Annotation score: | ||
H0Y7H1 | H0Y7H1_HUMAN | alpha-1,2-Mannosidase | MAN1A2 | 331 | Annotation score: | ||
Q5T9E3 | Q5T9E3_HUMAN | Mannosyl-oligosaccharide 1,2-alpha-... | MAN1A2 | 97 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF053626 , AF053622, AF053623, AF053624, AF053625 Genomic DNA Translation: AAC26201.1 AF027156 mRNA Translation: AAC26169.1 AF053621 , AF053619, AF053620, AF053615, AF053616, AF053617, AF053618 Genomic DNA Translation: AAC26200.1 AL157902 Genomic DNA No translation available. AL358072 Genomic DNA No translation available. BC063300 mRNA Translation: AAH63300.1 |
CCDSi | CCDS895.1 |
RefSeqi | NP_006690.1, NM_006699.3 |
Genome annotation databases
Ensembli | ENST00000356554; ENSP00000348959; ENSG00000198162 |
GeneIDi | 10905 |
KEGGi | hsa:10905 |
MANE-Selecti | ENST00000356554.7; ENSP00000348959.3; NM_006699.5; NP_006690.1 |
UCSCi | uc001ehd.2, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF053626 , AF053622, AF053623, AF053624, AF053625 Genomic DNA Translation: AAC26201.1 AF027156 mRNA Translation: AAC26169.1 AF053621 , AF053619, AF053620, AF053615, AF053616, AF053617, AF053618 Genomic DNA Translation: AAC26200.1 AL157902 Genomic DNA No translation available. AL358072 Genomic DNA No translation available. BC063300 mRNA Translation: AAH63300.1 |
CCDSi | CCDS895.1 |
RefSeqi | NP_006690.1, NM_006699.3 |
3D structure databases
SMRi | O60476 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 116111, 34 interactors |
IntActi | O60476, 19 interactors |
MINTi | O60476 |
STRINGi | 9606.ENSP00000348959 |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
PTM databases
GlyGeni | O60476, 1 site |
iPTMneti | O60476 |
PhosphoSitePlusi | O60476 |
Genetic variation databases
BioMutai | MAN1A2 |
Proteomic databases
EPDi | O60476 |
jPOSTi | O60476 |
MassIVEi | O60476 |
MaxQBi | O60476 |
PaxDbi | O60476 |
PeptideAtlasi | O60476 |
PRIDEi | O60476 |
ProteomicsDBi | 49416 |
Protocols and materials databases
Antibodypediai | 33890, 95 antibodies from 25 providers |
DNASUi | 10905 |
Genome annotation databases
Ensembli | ENST00000356554; ENSP00000348959; ENSG00000198162 |
GeneIDi | 10905 |
KEGGi | hsa:10905 |
MANE-Selecti | ENST00000356554.7; ENSP00000348959.3; NM_006699.5; NP_006690.1 |
UCSCi | uc001ehd.2, human |
Organism-specific databases
CTDi | 10905 |
DisGeNETi | 10905 |
GeneCardsi | MAN1A2 |
HGNCi | HGNC:6822, MAN1A2 |
HPAi | ENSG00000198162, Low tissue specificity |
MIMi | 604345, gene |
neXtProti | NX_O60476 |
OpenTargetsi | ENSG00000198162 |
PharmGKBi | PA30571 |
VEuPathDBi | HostDB:ENSG00000198162 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2204, Eukaryota |
GeneTreei | ENSGT00940000157498 |
HOGENOMi | CLU_003818_3_2_1 |
InParanoidi | O60476 |
OMAi | YSYLIHA |
OrthoDBi | 693882at2759 |
PhylomeDBi | O60476 |
TreeFami | TF313420 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BRENDAi | 3.2.1.113, 2681 |
PathwayCommonsi | O60476 |
Reactomei | R-HSA-6811438, Intra-Golgi traffic R-HSA-964827, Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2 |
SignaLinki | O60476 |
Miscellaneous databases
BioGRID-ORCSi | 10905, 16 hits in 1048 CRISPR screens |
ChiTaRSi | MAN1A2, human |
GeneWikii | MAN1A2 |
GenomeRNAii | 10905 |
Pharosi | O60476, Tbio |
PROi | PR:O60476 |
RNActi | O60476, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000198162, Expressed in chorionic villus and 247 other tissues |
ExpressionAtlasi | O60476, baseline and differential |
Genevisiblei | O60476, HS |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MA1A2_HUMAN | |
Accessioni | O60476Primary (citable) accession number: O60476 Secondary accession number(s): Q9H510 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 16, 2001 |
Last sequence update: | August 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 180 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families