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Protein

Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB

Gene

MAN1A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man9GlcNAc2 to produce Man5GlcNAc2.

Catalytic activityi

Hydrolysis of the terminal (1->2)-linked alpha-D-mannose residues in the oligo-mannose oligosaccharide Man9(GlcNAc)2.By similarity

Cofactori

Ca2+By similarity

Activity regulationi

Inhibited by both 1-deoxymannojirimycin and kifunensine.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei508Proton donorBy similarity1
Metal bindingi619CalciumBy similarity1

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase
LigandCalcium, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS05846-MONOMER
BRENDAi3.2.1.113 2681
ReactomeiR-HSA-6811438 Intra-Golgi traffic
R-HSA-964827 Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2
UniPathwayi
UPA00378

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

Names & Taxonomyi

Protein namesi
Recommended name:
Mannosyl-oligosaccharide 1,2-alpha-mannosidase IB (EC:3.2.1.113By similarity)
Alternative name(s):
Mannosidase alpha class 1A member 2
Processing alpha-1,2-mannosidase IB
Short name:
Alpha-1,2-mannosidase IB
Gene namesi
Name:MAN1A2
Synonyms:MAN1B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

EuPathDBiHostDB:ENSG00000198162.12
HGNCiHGNC:6822 MAN1A2
MIMi604345 gene
neXtProtiNX_O60476

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 36CytoplasmicSequence analysisAdd BLAST35
Transmembranei37 – 57Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini58 – 641LumenalSequence analysisAdd BLAST584

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi10905
OpenTargetsiENSG00000198162
PharmGKBiPA30571

Polymorphism and mutation databases

BioMutaiMAN1A2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00002103122 – 641Mannosyl-oligosaccharide 1,2-alpha-mannosidase IBAdd BLAST640

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylthreonineCombined sources1
Disulfide bondi462 ↔ 494By similarity
Glycosylationi631N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein

Proteomic databases

EPDiO60476
MaxQBiO60476
PaxDbiO60476
PeptideAtlasiO60476
PRIDEiO60476
ProteomicsDBi49416

PTM databases

iPTMnetiO60476
PhosphoSitePlusiO60476

Expressioni

Tissue specificityi

Highest levels of expression in placenta and testis.

Gene expression databases

BgeeiENSG00000198162 Expressed in 234 organ(s), highest expression level in chorionic villus
CleanExiHS_MAN1A2
ExpressionAtlasiO60476 baseline and differential
GenevisibleiO60476 HS

Organism-specific databases

HPAiHPA034559
HPA057364

Interactioni

Protein-protein interaction databases

BioGridi116111, 19 interactors
IntActiO60476, 4 interactors
MINTiO60476
STRINGi9606.ENSP00000348959

Structurei

3D structure databases

ProteinModelPortaliO60476
SMRiO60476
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 47 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG2431 Eukaryota
ENOG410XP04 LUCA
GeneTreeiENSGT00390000016529
HOGENOMiHOG000181988
HOVERGENiHBG052389
InParanoidiO60476
KOiK01230
OMAiQYGWGHN
OrthoDBiEOG091G04C8
PhylomeDBiO60476
TreeFamiTF313420

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

O60476-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTTPALLPLS GRRIPPLNLG PPSFPHHRAT LRLSEKFILL LILSAFITLC
60 70 80 90 100
FGAFFFLPDS SKHKRFDLGL EDVLIPHVDA GKGAKNPGVF LIHGPDEHRH
110 120 130 140 150
REEEERLRNK IRADHEKALE EAKEKLRKSR EEIRAEIQTE KNKVVQEMKI
160 170 180 190 200
KENKPLPPVP IPNLVGIRGG DPEDNDIREK REKIKEMMKH AWDNYRTYGW
210 220 230 240 250
GHNELRPIAR KGHSPNIFGS SQMGATIVDA LDTLYIMGLH DEFLDGQRWI
260 270 280 290 300
EDNLDFSVNS EVSVFEVNIR FIGGLLAAYY LSGEEIFKIK AVQLAEKLLP
310 320 330 340 350
AFNTPTGIPW AMVNLKSGVG RNWGWASAGS SILAEFGTLH MEFIHLSYLT
360 370 380 390 400
GDLTYYKKVM HIRKLLQKMD RPNGLYPNYL NPRTGRWGQY HTSVGGLGDS
410 420 430 440 450
FYEYLLKAWL MSDKTDHEAR KMYDDAIEAI EKHLIKKSRG GLTFIGEWKN
460 470 480 490 500
GHLEKKMGHL ACFAGGMFAL GADGSRADKA GHYLELGAEI ARTCHESYDR
510 520 530 540 550
TALKLGPESF KFDGAVEAVA VRQAEKYYIL RPEVIETYWY LWRFTHDPRY
560 570 580 590 600
RQWGWEAALA IEKYCRVNGG FSGVKDVYSS TPTHDDVQQS FFLAETLKYL
610 620 630 640
YLLFSGDDLL PLDHWVFNTE AHPLPVLHLA NTTLSGNPAV R
Length:641
Mass (Da):73,004
Last modified:August 1, 1998 - v1
Checksum:i19DA691A9C4B0ED3
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H0Y7H1H0Y7H1_HUMAN
alpha-1,2-Mannosidase
MAN1A2
331Annotation score:
H0Y543H0Y543_HUMAN
alpha-1,2-Mannosidase
MAN1A2
204Annotation score:
Q5T9E3Q5T9E3_HUMAN
Mannosyl-oligosaccharide 1,2-alpha-...
MAN1A2
97Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053626
, AF053622, AF053623, AF053624, AF053625 Genomic DNA Translation: AAC26201.1
AF027156 mRNA Translation: AAC26169.1
AF053621
, AF053619, AF053620, AF053615, AF053616, AF053617, AF053618 Genomic DNA Translation: AAC26200.1
AL157902 Genomic DNA No translation available.
AL358072 Genomic DNA No translation available.
BC063300 mRNA Translation: AAH63300.1
CCDSiCCDS895.1
RefSeqiNP_006690.1, NM_006699.3
UniGeneiHs.435938
Hs.602811

Genome annotation databases

EnsembliENST00000356554; ENSP00000348959; ENSG00000198162
GeneIDi10905
KEGGihsa:10905
UCSCiuc001ehd.2 human

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053626
, AF053622, AF053623, AF053624, AF053625 Genomic DNA Translation: AAC26201.1
AF027156 mRNA Translation: AAC26169.1
AF053621
, AF053619, AF053620, AF053615, AF053616, AF053617, AF053618 Genomic DNA Translation: AAC26200.1
AL157902 Genomic DNA No translation available.
AL358072 Genomic DNA No translation available.
BC063300 mRNA Translation: AAH63300.1
CCDSiCCDS895.1
RefSeqiNP_006690.1, NM_006699.3
UniGeneiHs.435938
Hs.602811

3D structure databases

ProteinModelPortaliO60476
SMRiO60476
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi116111, 19 interactors
IntActiO60476, 4 interactors
MINTiO60476
STRINGi9606.ENSP00000348959

Protein family/group databases

CAZyiGH47 Glycoside Hydrolase Family 47

PTM databases

iPTMnetiO60476
PhosphoSitePlusiO60476

Polymorphism and mutation databases

BioMutaiMAN1A2

Proteomic databases

EPDiO60476
MaxQBiO60476
PaxDbiO60476
PeptideAtlasiO60476
PRIDEiO60476
ProteomicsDBi49416

Protocols and materials databases

DNASUi10905
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000356554; ENSP00000348959; ENSG00000198162
GeneIDi10905
KEGGihsa:10905
UCSCiuc001ehd.2 human

Organism-specific databases

CTDi10905
DisGeNETi10905
EuPathDBiHostDB:ENSG00000198162.12
GeneCardsiMAN1A2
HGNCiHGNC:6822 MAN1A2
HPAiHPA034559
HPA057364
MIMi604345 gene
neXtProtiNX_O60476
OpenTargetsiENSG00000198162
PharmGKBiPA30571
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2431 Eukaryota
ENOG410XP04 LUCA
GeneTreeiENSGT00390000016529
HOGENOMiHOG000181988
HOVERGENiHBG052389
InParanoidiO60476
KOiK01230
OMAiQYGWGHN
OrthoDBiEOG091G04C8
PhylomeDBiO60476
TreeFamiTF313420

Enzyme and pathway databases

UniPathwayi
UPA00378

BioCyciMetaCyc:HS05846-MONOMER
BRENDAi3.2.1.113 2681
ReactomeiR-HSA-6811438 Intra-Golgi traffic
R-HSA-964827 Progressive trimming of alpha-1,2-linked mannose residues from Man9/8/7GlcNAc2 to produce Man5GlcNAc2

Miscellaneous databases

ChiTaRSiMAN1A2 human
GeneWikiiMAN1A2
GenomeRNAii10905
PROiPR:O60476
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000198162 Expressed in 234 organ(s), highest expression level in chorionic villus
CleanExiHS_MAN1A2
ExpressionAtlasiO60476 baseline and differential
GenevisibleiO60476 HS

Family and domain databases

Gene3Di1.50.10.10, 1 hit
InterProiView protein in InterPro
IPR012341 6hp_glycosidase-like_sf
IPR001382 Glyco_hydro_47
IPR036026 Seven-hairpin_glycosidases
PfamiView protein in Pfam
PF01532 Glyco_hydro_47, 1 hit
PRINTSiPR00747 GLYHDRLASE47
SUPFAMiSSF48225 SSF48225, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMA1A2_HUMAN
AccessioniPrimary (citable) accession number: O60476
Secondary accession number(s): Q9H510
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: August 1, 1998
Last modified: November 7, 2018
This is version 162 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  3. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  4. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  5. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
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