UniProtKB - O60447 (EVI5_HUMAN)
Ecotropic viral integration site 5 protein homolog
EVI5
Functioni
Functions as a regulator of cell cycle progression by stabilizing the FBXO5 protein and promoting cyclin-A accumulation during interphase. May play a role in cytokinesis.
1 PublicationMiscellaneous
GO - Molecular functioni
- GTPase activator activity Source: UniProtKB
- small GTPase binding Source: UniProtKB
GO - Biological processi
- activation of GTPase activity Source: GO_Central
- cell cycle Source: UniProtKB-KW
- cell division Source: UniProtKB-KW
- intracellular protein transport Source: GO_Central
- positive regulation of GTPase activity Source: UniProtKB
- retrograde transport, endosome to Golgi Source: UniProtKB
Keywordsi
Biological process | Cell cycle, Cell division |
Enzyme and pathway databases
PathwayCommonsi | O60447 |
SignaLinki | O60447 |
SIGNORi | O60447 |
Names & Taxonomyi
Protein namesi | Recommended name: Ecotropic viral integration site 5 protein homologShort name: EVI-5 Alternative name(s): Neuroblastoma stage 4S gene protein |
Gene namesi | Name:EVI5 Synonyms:NB4S |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:3501, EVI5 |
MIMi | 602942, gene |
neXtProti | NX_O60447 |
VEuPathDBi | HostDB:ENSG00000067208 |
Subcellular locationi
Nucleus
Cytoskeleton
Note: Associates with the mitotic spindle through anaphase and remains within the midzone and midbody until completion of cytokinesis.
Cytoskeleton
- microtubule organizing center Source: UniProtKB-SubCell
- spindle Source: UniProtKB-SubCell
Cytosol
- cytosol Source: UniProtKB
Nucleus
- nucleus Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, Cytoskeleton, NucleusPathology & Biotechi
Involvement in diseasei
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 594 | Breakpoint for translocation to form EVI5-TRNG10 fusion protein | 1 |
Organism-specific databases
DisGeNETi | 7813 |
OpenTargetsi | ENSG00000067208 |
PharmGKBi | PA27915 |
Miscellaneous databases
Pharosi | O60447, Tbio |
Genetic variation databases
BioMutai | EVI5 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000256241 | 1 – 810 | Ecotropic viral integration site 5 protein homologAdd BLAST | 810 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 102 | PhosphoserineCombined sources | 1 | |
Modified residuei | 113 | PhosphoserineBy similarity | 1 | |
Modified residuei | 497 | PhosphoserineCombined sources | 1 | |
Modified residuei | 689 | PhosphoserineCombined sources | 1 | |
Modified residuei | 776 | PhosphoserineCombined sources | 1 | |
Modified residuei | 778 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Phosphoprotein, Ubl conjugationProteomic databases
EPDi | O60447 |
jPOSTi | O60447 |
MassIVEi | O60447 |
MaxQBi | O60447 |
PaxDbi | O60447 |
PeptideAtlasi | O60447 |
PRIDEi | O60447 |
ProteomicsDBi | 49405 [O60447-1] |
PTM databases
iPTMneti | O60447 |
PhosphoSitePlusi | O60447 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
Bgeei | ENSG00000067208, Expressed in calcaneal tendon and 233 other tissues |
ExpressionAtlasi | O60447, baseline and differential |
Genevisiblei | O60447, HS |
Organism-specific databases
HPAi | ENSG00000067208, Low tissue specificity |
Interactioni
Subunit structurei
Dimeric and monomeric.
Interacts with alpha- and gamma-tubulin.
Interacts with FBXO5.
Interacts with the chromosome passenger complex (CPC) which is at least composed of AURKB/aurora-B, BIRC5/survivin, CDCA8/borealin and INCENP.
3 PublicationsBinary interactionsi
O60447
GO - Molecular functioni
- small GTPase binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 113584, 52 interactors |
CORUMi | O60447 |
DIPi | DIP-38024N |
IntActi | O60447, 38 interactors |
STRINGi | 9606.ENSP00000359356 |
Miscellaneous databases
RNActi | O60447, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 163 – 348 | Rab-GAP TBCPROSITE-ProRule annotationAdd BLAST | 186 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 483 | Interaction with alpha-tubulin, gamma-tubulin, BIRC5 and FBXO52 PublicationsAdd BLAST | 483 | |
Regioni | 49 – 80 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 98 – 123 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 128 – 693 | DimerizationAdd BLAST | 566 | |
Regioni | 377 – 810 | Targeting to the centrosomesAdd BLAST | 434 | |
Regioni | 487 – 810 | Interaction with AURKB and INCENP1 PublicationAdd BLAST | 324 | |
Regioni | 756 – 810 | DisorderedSequence analysisAdd BLAST | 55 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 406 – 716 | Sequence analysisAdd BLAST | 311 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 99 – 123 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 781 – 804 | Basic and acidic residuesSequence analysisAdd BLAST | 24 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG4436, Eukaryota |
GeneTreei | ENSGT00940000153846 |
HOGENOMi | CLU_005350_6_0_1 |
InParanoidi | O60447 |
OMAi | NSKKMKX |
OrthoDBi | 191811at2759 |
PhylomeDBi | O60447 |
TreeFami | TF317184 |
Family and domain databases
InterProi | View protein in InterPro IPR031214, Evi5 IPR000195, Rab-GTPase-TBC_dom IPR035969, Rab-GTPase_TBC_sf |
PANTHERi | PTHR22957:SF254, PTHR22957:SF254, 2 hits |
Pfami | View protein in Pfam PF00566, RabGAP-TBC, 1 hit |
SMARTi | View protein in SMART SM00164, TBC, 1 hit |
SUPFAMi | SSF47923, SSF47923, 2 hits |
PROSITEi | View protein in PROSITE PS50086, TBC_RABGAP, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVTNKMTAAF RNPSGKQVAT DKVAEKLSST LSWVKNTVSH TVSQMASQVA
60 70 80 90 100
SPSTSLHTTS SSTTLSTPAL SPSSPSQLSP DDLELLAKLE EQNRLLETDS
110 120 130 140 150
KSLRSVNGSR RNSGSSLVSS SSASSNLSHL EEDSWILWGR IVNEWEDVRK
160 170 180 190 200
KKEKQVKELV HKGIPHHFRA IVWQLLCSAQ SMPIKDQYSE LLKMTSPCEK
210 220 230 240 250
LIRRDIARTY PEHNFFKEKD SLGQEVLFNV MKAYSLVDRE VGYCQGSAFI
260 270 280 290 300
VGLLLMQMPE EEAFCVFVKL MQDYRLRELF KPSMAELGLC MYQFECMIQE
310 320 330 340 350
HLPELFVHFQ SQSFHTSMYA SSWFLTIFLT TFPLPIATRI FDIFMSEGLE
360 370 380 390 400
IVFRVGLALL QMNQAELMQL DMEGMLQHFQ KVIPHQFDGV PDKLIQAAYQ
410 420 430 440 450
VKYNSKKMKK LEKEYTTIKT KEMEEQVEIK RLRTENRLLK QRIETLEKHK
460 470 480 490 500
CSSNYNEDFV LQLEKELVQA RLSEAESQCA LKEMQDKVLD IEKRNNSLPD
510 520 530 540 550
ENNIARLQEE LIAVKLREAE AIMGLKELRQ QVKDLEEHWQ RHLARTTGRW
560 570 580 590 600
KDPPKKNAMN ELQDELMTIR LREAETQAEI REIKQRMMEM ETQNQINSNH
610 620 630 640 650
LRRAEQEVIS LQEKVQYLSA QNKGLLTQLS EAKRKQAEIE CKNKEEVMAV
660 670 680 690 700
RLREADSIAA VAELRQHIAE LEIQKEEGKL QGQLNKSDSN QYIGELKDQI
710 720 730 740 750
AELNHELRCL KGQRGFSGQP PFDGIHIVNH LIGDDESFHS SDEDFIDNSL
760 770 780 790 800
QETGVGFPLH GKSGSMSLDP AVADGSESET EDSVLETRES NQVVQKERPP
810
RRRESYSTTV
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A804HIC4 | A0A804HIC4_HUMAN | Ecotropic viral integration site 5 ... | EVI5 | 826 | Annotation score: | ||
A0A1B0GUF4 | A0A1B0GUF4_HUMAN | Ecotropic viral integration site 5 ... | EVI5 | 324 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 94 | R → I in AAC16031 (PubMed:9618176).Curated | 1 | |
Sequence conflicti | 94 | R → I in BAH16618 (PubMed:19077034).Curated | 1 | |
Sequence conflicti | 402 | K → Q in AAC16031 (PubMed:9618176).Curated | 1 | |
Sequence conflicti | 714 | R → K in AAC16031 (PubMed:9618176).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_047753 | 82 | D → V2 PublicationsCorresponds to variant dbSNP:rs1064580Ensembl. | 1 | |
Natural variantiVAR_028890 | 336 | I → V2 PublicationsCorresponds to variant dbSNP:rs2391199Ensembl. | 1 | |
Natural variantiVAR_028891 | 612 | Q → H. Corresponds to variant dbSNP:rs11808092Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_056828 | 448 | K → KESASLADRLIQ in isoform 2. 1 Publication | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF008915 mRNA Translation: AAC16031.1 AB449875 mRNA Translation: BAH16618.1 AC104332 Genomic DNA No translation available. AC104456 Genomic DNA No translation available. AL133332 Genomic DNA No translation available. AL354890 Genomic DNA No translation available. |
CCDSi | CCDS30774.1 [O60447-1] CCDS76179.1 [O60447-2] |
RefSeqi | NP_001295177.1, NM_001308248.1 [O60447-2] NP_005656.4, NM_005665.5 [O60447-1] |
Genome annotation databases
Ensembli | ENST00000370331; ENSP00000359356; ENSG00000067208 ENST00000540033; ENSP00000440826; ENSG00000067208 [O60447-2] |
GeneIDi | 7813 |
KEGGi | hsa:7813 |
UCSCi | uc001dox.3, human [O60447-1] |
Keywords - Coding sequence diversityi
Alternative splicing, Chromosomal rearrangementSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF008915 mRNA Translation: AAC16031.1 AB449875 mRNA Translation: BAH16618.1 AC104332 Genomic DNA No translation available. AC104456 Genomic DNA No translation available. AL133332 Genomic DNA No translation available. AL354890 Genomic DNA No translation available. |
CCDSi | CCDS30774.1 [O60447-1] CCDS76179.1 [O60447-2] |
RefSeqi | NP_001295177.1, NM_001308248.1 [O60447-2] NP_005656.4, NM_005665.5 [O60447-1] |
3D structure databases
SMRi | O60447 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 113584, 52 interactors |
CORUMi | O60447 |
DIPi | DIP-38024N |
IntActi | O60447, 38 interactors |
STRINGi | 9606.ENSP00000359356 |
PTM databases
iPTMneti | O60447 |
PhosphoSitePlusi | O60447 |
Genetic variation databases
BioMutai | EVI5 |
Proteomic databases
EPDi | O60447 |
jPOSTi | O60447 |
MassIVEi | O60447 |
MaxQBi | O60447 |
PaxDbi | O60447 |
PeptideAtlasi | O60447 |
PRIDEi | O60447 |
ProteomicsDBi | 49405 [O60447-1] |
Protocols and materials databases
Antibodypediai | 19897, 84 antibodies from 20 providers |
DNASUi | 7813 |
Genome annotation databases
Ensembli | ENST00000370331; ENSP00000359356; ENSG00000067208 ENST00000540033; ENSP00000440826; ENSG00000067208 [O60447-2] |
GeneIDi | 7813 |
KEGGi | hsa:7813 |
UCSCi | uc001dox.3, human [O60447-1] |
Organism-specific databases
CTDi | 7813 |
DisGeNETi | 7813 |
GeneCardsi | EVI5 |
HGNCi | HGNC:3501, EVI5 |
HPAi | ENSG00000067208, Low tissue specificity |
MIMi | 602942, gene |
neXtProti | NX_O60447 |
OpenTargetsi | ENSG00000067208 |
PharmGKBi | PA27915 |
VEuPathDBi | HostDB:ENSG00000067208 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG4436, Eukaryota |
GeneTreei | ENSGT00940000153846 |
HOGENOMi | CLU_005350_6_0_1 |
InParanoidi | O60447 |
OMAi | NSKKMKX |
OrthoDBi | 191811at2759 |
PhylomeDBi | O60447 |
TreeFami | TF317184 |
Enzyme and pathway databases
PathwayCommonsi | O60447 |
SignaLinki | O60447 |
SIGNORi | O60447 |
Miscellaneous databases
BioGRID-ORCSi | 7813, 5 hits in 1050 CRISPR screens |
ChiTaRSi | EVI5, human |
GeneWikii | EVI5 |
GenomeRNAii | 7813 |
Pharosi | O60447, Tbio |
PROi | PR:O60447 |
RNActi | O60447, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000067208, Expressed in calcaneal tendon and 233 other tissues |
ExpressionAtlasi | O60447, baseline and differential |
Genevisiblei | O60447, HS |
Family and domain databases
InterProi | View protein in InterPro IPR031214, Evi5 IPR000195, Rab-GTPase-TBC_dom IPR035969, Rab-GTPase_TBC_sf |
PANTHERi | PTHR22957:SF254, PTHR22957:SF254, 2 hits |
Pfami | View protein in Pfam PF00566, RabGAP-TBC, 1 hit |
SMARTi | View protein in SMART SM00164, TBC, 1 hit |
SUPFAMi | SSF47923, SSF47923, 2 hits |
PROSITEi | View protein in PROSITE PS50086, TBC_RABGAP, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | EVI5_HUMAN | |
Accessioni | O60447Primary (citable) accession number: O60447 Secondary accession number(s): A6NKX8, B9A6J0, Q9H1Y9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 31, 2006 |
Last sequence update: | November 30, 2010 | |
Last modified: | February 23, 2022 | |
This is version 163 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 1
Human chromosome 1: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot