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Protein

Germinal-center associated nuclear protein

Gene

MCM3AP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Isoform GANP: Essential for the generation of high-affinity B-cells against T-cell-dependent antigens by affecting somatic hypermutation at the IgV-regions. May have stimulation-dependent DNA primase activity that would generate extra RNA primers in very rapidely proliferating cells and would support clonal expansion of differentiating B-cells (By similarity). Involved in the nuclear export of poly(A)-containing mRNAs by acting as a scaffold for the TREX-2 complex. The TREX-2 complex functions in docking export-competent ribonucleoprotein particles (mRNPs) to the nuclear entrance of the nuclear pore complex (nuclear basket). TREX-2 participates in mRNA export and accurate chromatin positioning in the nucleus by tethering genes to the nuclear periphery.By similarity1 Publication
Isoform MCM3AP: Acetyltransferase targeting MCM3. Inhibits initiation of DNA replication, but not elongation.1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processImmunity, mRNA transport, Protein transport, Translocation, Transport

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Germinal-center associated nuclear protein
Short name:
GANP
Alternative name(s):
80 kDa MCM3-associated protein
MCM3 acetylating protein (EC:2.3.1.-)
Short name:
MCM3AP
MCM3 acetyltransferase
Gene namesi
Name:MCM3AP
Synonyms:GANP, KIAA0572, MAP80
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 21

Organism-specific databases

EuPathDBiHostDB:ENSG00000160294.10
HGNCiHGNC:6946 MCM3AP
MIMi603294 gene
neXtProtiNX_O60318

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nuclear pore complex, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1730 – 1733HGAG → AAAA: Severely decreased MCM3 acetylating activity. 1 Publication4

Organism-specific databases

DisGeNETi8888
MalaCardsiMCM3AP
OpenTargetsiENSG00000160294
PharmGKBiPA30692

Polymorphism and mutation databases

BioMutaiMCM3AP

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000962841 – 1980Germinal-center associated nuclear proteinAdd BLAST1980

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei32Asymmetric dimethylarginineBy similarity1
Modified residuei430PhosphoserineCombined sources1
Modified residuei489N6-acetyllysineBy similarity1
Modified residuei490N6-acetyllysineBy similarity1
Modified residuei508PhosphoserineBy similarity1
Modified residuei538PhosphoserineCombined sources1
Modified residuei557PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation at Ser-508 by CDKs is required for DNA primase activity.By similarity

Keywords - PTMi

Acetylation, Methylation, Phosphoprotein

Proteomic databases

EPDiO60318
MaxQBiO60318
PaxDbiO60318
PeptideAtlasiO60318
PRIDEiO60318
ProteomicsDBi49343

PTM databases

iPTMnetiO60318
PhosphoSitePlusiO60318

Expressioni

Gene expression databases

BgeeiENSG00000160294 Expressed in 222 organ(s), highest expression level in tendon
CleanExiHS_MCM3AP
ExpressionAtlasiO60318 baseline and differential
GenevisibleiO60318 HS

Organism-specific databases

HPAiHPA021527
HPA032103

Interactioni

Subunit structurei

Interacts with AICDA/AID (By similarity). Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least GANP, two copies of ENY2, PCID2, SEM1, and either centrin CETN2 or CETN3. Isoform MCM3AP interacts with MCM3. Interacts with NXF1 (By via FG-repeats).By similarity3 Publications

Protein-protein interaction databases

BioGridi114406, 61 interactors
DIPiDIP-31696N
IntActiO60318, 38 interactors
MINTiO60318
STRINGi9606.ENSP00000291688

Structurei

Secondary structure

11980
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO60318
SMRiO60318
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini775 – 958PCIPROSITE-ProRule annotationAdd BLAST184

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni33 – 340FG-repeatsAdd BLAST308
Regioni421 – 557DNA primaseAdd BLAST137
Regioni1162 – 1256CIDAdd BLAST95
Regioni1658 – 1790AcetyltransferaseAdd BLAST133

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili1133 – 1170Sequence analysisAdd BLAST38

Sequence similaritiesi

Belongs to the SAC3 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1860 Eukaryota
COG5079 LUCA
GeneTreeiENSGT00530000063781
HOGENOMiHOG000113500
HOVERGENiHBG102020
InParanoidiO60318
OMAiNSSEVKF
OrthoDBiEOG091G01V4
PhylomeDBiO60318
TreeFamiTF105948

Family and domain databases

CDDicd12443 RRM_MCM3A_like, 1 hit
InterProiView protein in InterPro
IPR031910 GANP_CID_dom
IPR031907 MCM3AP_GANP
IPR034265 MCM3AP_RRM
IPR031908 NupH_GANP
IPR000717 PCI_dom
IPR035979 RBD_domain_sf
IPR005062 SAC3/GANP/THP3
PfamiView protein in Pfam
PF16766 CID_GANP, 1 hit
PF16769 MCM3AP_GANP, 1 hit
PF16768 NupH_GANP, 1 hit
PF03399 SAC3_GANP, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform GANP (identifier: O60318-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MNPTNPFSGQ QPSAFSASSS NVGTLPSKPP FRFGQPSLFG QNSTLSGKSS
60 70 80 90 100
GFSQVSSFPA SSGVSHSSSV QTLGFTQTSS VGPFSGLEHT STFVATSGPS
110 120 130 140 150
SSSVLGNTGF SFKSPTSVGA FPSTSAFGQE AGEIVNSGFG KTEFSFKPLE
160 170 180 190 200
NAVFKPILGA ESEPEKTQSQ IASGFFTFSH PISSAPGGLA PFSFPQVTSS
210 220 230 240 250
SATTSNFTFS KPVSSNNSLS AFTPALSNQN VEEEKRGPKS IFGSSNNSFS
260 270 280 290 300
SFPVSSAVLG EPFQASKAGV RQGCEEAVSQ VEPLPSLMKG LKRKEDQDRS
310 320 330 340 350
PRRHGHEPAE DSDPLSRGDH PPDKRPVRLN RPRGGTLFGR TIQDVFKSNK
360 370 380 390 400
EVGRLGNKEA KKETGFVESA ESDHMAIPGG NQSVLAPSRI PGVNKEEETE
410 420 430 440 450
SREKKEDSLR GTPARQSNRS ESTDSLGGLS PSEVTAIQCK NIPDYLNDRT
460 470 480 490 500
ILENHFGKIA KVQRIFTRRS KKLAVVHFFD HASAALARKK GKSLHKDMAI
510 520 530 540 550
FWHRKKISPN KKPFSLKEKK PGDGEVSPST EDAPFQHSPL GKAAGRTGAS
560 570 580 590 600
SLLNKSSPVK KPSLLKAHQF EGDSFDSASE GSEGLGPCVL SLSTLIGTVA
610 620 630 640 650
ETSKEKYRLL DQRDRIMRQA RVKRTDLDKA RTFVGTCLDM CPEKERYMRE
660 670 680 690 700
TRSQLSVFEV VPGTDQVDHA AAVKEYSRSS ADQEEPLPHE LRPLPVLSRT
710 720 730 740 750
MDYLVTQIMD QKEGSLRDWY DFVWNRTRGI RKDITQQHLC DPLTVSLIEK
760 770 780 790 800
CTRFHIHCAH FMCEEPMSSF DAKINNENMT KCLQSLKEMY QDLRNKGVFC
810 820 830 840 850
ASEAEFQGYN VLLSLNKGDI LREVQQFHPA VRNSSEVKFA VQAFAALNSN
860 870 880 890 900
NFVRFFKLVQ SASYLNACLL HCYFSQIRKD ALRALNFAYT VSTQRSTIFP
910 920 930 940 950
LDGVVRMLLF RDCEEATDFL TCHGLTVSDG CVELNRSAFL EPEGLSKTRK
960 970 980 990 1000
SVFITRKLTV SVGEIVNGGP LPPVPRHTPV CSFNSQNKYI GESLAAELPV
1010 1020 1030 1040 1050
STQRPGSDTV GGGRGEECGV EPDAPLSSLP QSLPAPAPSP VPLPPVLALT
1060 1070 1080 1090 1100
PSVAPSLFQL SVQPEPPPPE PVPMYSDEDL AQVVDELIQE ALQRDCEEVG
1110 1120 1130 1140 1150
SAGAAYAAAA LGVSNAAMED LLTAATTGIL RHIAAEEVSK ERERREQERQ
1160 1170 1180 1190 1200
RAEEERLKQE RELVLSELSQ GLAVELMERV MMEFVRETCS QELKNAVETD
1210 1220 1230 1240 1250
QRVRVARCCE DVCAHLVDLF LVEEIFQTAK ETLQELQCFC KYLQRWREAV
1260 1270 1280 1290 1300
TARKKLRRQM RAFPAAPCCV DVSDRLRALA PSAECPIAEE NLARGLLDLG
1310 1320 1330 1340 1350
HAGRLGISCT RLRRLRNKTA HQMKVQHFYQ QLLSDVAWAS LDLPSLVAEH
1360 1370 1380 1390 1400
LPGRQEHVFW KLVLVLPDVE EQSPESCGRI LANWLKVKFM GDEGSVDDTS
1410 1420 1430 1440 1450
SDAGGIQTLS LFNSLSSKGD QMISVNVCIK VAHGALSDGA IDAVETQKDL
1460 1470 1480 1490 1500
LGASGLMLLL PPKMKSEDMA EEDVYWLSAL LQLKQLLQAK PFQPALPLVV
1510 1520 1530 1540 1550
LVPSPGGDAV EKEVEDGLML QDLVSAKLIS DYTVTEIPDT INDLQGSTKV
1560 1570 1580 1590 1600
LQAVQWLVSH CPHSLDLCCQ TLIQYVEDGI GHEFSGRFFH DRRERRLGGL
1610 1620 1630 1640 1650
ASQEPGAIIE LFNSVLQFLA SVVSSEQLCD LSWPVTEFAE AGGSRLLPHL
1660 1670 1680 1690 1700
HWNAPEHLAW LKQAVLGFQL PQMDLPPLGA PWLPVCSMVV QYASQIPSSR
1710 1720 1730 1740 1750
QTQPVLQSQV ENLLHRTYCR WKSKSPSPVH GAGPSVMEIP WDDLIALCIN
1760 1770 1780 1790 1800
HKLRDWTPPR LPVTSEALSE DGQICVYFFK NDLKKYDVPL SWEQARLQTQ
1810 1820 1830 1840 1850
KELQLREGRL AIKPFHPSAN NFPIPLLHMH RNWKRSTECA QEGRIPSTED
1860 1870 1880 1890 1900
LMRGASAEEL LAQCLSSSLL LEKEENKRFE DQLQQWLSED SGAFTDLTSL
1910 1920 1930 1940 1950
PLYLPQTLVS LSHTIEPVMK TSVTTSPQSD MMREQLQLSE ATGTCLGERL
1960 1970 1980
KHLERLIRSS REEEVASELH LSALLDMVDI
Length:1,980
Mass (Da):218,405
Last modified:May 30, 2000 - v2
Checksum:i503D192686FC38A8
GO
Isoform MCM3AP (identifier: O60318-2) [UniParc]FASTAAdd to basket
Also known as: 80 kDa MCM3-associated protein

The sequence of this isoform differs from the canonical sequence as follows:
     1-1259: Missing.

Note: Produced via an alternative promoter within an intron of GANP. MCM3AP promoter elements are poorly conserved in mice, suggesting that the regulation of MCM3AP may be human specific.
Show »
Length:721
Mass (Da):80,292
Checksum:i6D50E6EEBB6257C1
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0A0MSZ7A0A0A0MSZ7_HUMAN
Germinal-center-associated nuclear ...
MCM3AP
94Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_019240102S → L2 PublicationsCorresponds to variant dbSNP:rs9975588Ensembl.1
Natural variantiVAR_019241288M → V1 PublicationCorresponds to variant dbSNP:rs17182545Ensembl.1
Natural variantiVAR_019242333R → L1 PublicationCorresponds to variant dbSNP:rs17182552Ensembl.1
Natural variantiVAR_035472409L → V in a colorectal cancer sample; somatic mutation. 1 PublicationCorresponds to variant dbSNP:rs773228537Ensembl.1
Natural variantiVAR_019243413P → L1 PublicationCorresponds to variant dbSNP:rs17182566Ensembl.1
Natural variantiVAR_070560915E → K Found in a patient with border-line to mild intellectual disability, progressive polyneuropathy and ptosis; unknown pathological significance. 1 PublicationCorresponds to variant dbSNP:rs483352869EnsemblClinVar.1
Natural variantiVAR_0192441051P → L1 PublicationCorresponds to variant dbSNP:rs17182850Ensembl.1
Natural variantiVAR_0192451062V → M1 PublicationCorresponds to variant dbSNP:rs17182857Ensembl.1
Natural variantiVAR_0192461314R → W1 PublicationCorresponds to variant dbSNP:rs17176709Ensembl.1
Natural variantiVAR_0192471449D → E1 PublicationCorresponds to variant dbSNP:rs17183220Ensembl.1
Natural variantiVAR_0192481576V → I2 PublicationsCorresponds to variant dbSNP:rs17183248Ensembl.1
Natural variantiVAR_0192491795A → T1 PublicationCorresponds to variant dbSNP:rs17183290Ensembl.1
Natural variantiVAR_0539731831R → C. Corresponds to variant dbSNP:rs2298697Ensembl.1
Natural variantiVAR_0192501870L → R1 PublicationCorresponds to variant dbSNP:rs17176933Ensembl.1
Natural variantiVAR_0192511941A → V1 PublicationCorresponds to variant dbSNP:rs17183403Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0534381 – 1259Missing in isoform MCM3AP. CuratedAdd BLAST1259

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010089 mRNA Translation: CAB52687.1
AY590469 Genomic DNA Translation: AAS89300.1
AP000471 Genomic DNA No translation available.
AP001469 Genomic DNA No translation available.
BC013285 mRNA Translation: AAH13285.2
BC004497 mRNA Translation: AAH04497.2
BC104958 mRNA Translation: AAI04959.1
BC104960 mRNA Translation: AAI04961.1
AB011144 mRNA Translation: BAA25498.1
AB005543 mRNA Translation: BAA25170.1
CCDSiCCDS13734.1 [O60318-1]
PIRiT00339
RefSeqiNP_003897.2, NM_003906.4 [O60318-1]
XP_005261260.1, XM_005261203.4 [O60318-1]
XP_005261261.1, XM_005261204.4 [O60318-1]
XP_005261262.1, XM_005261205.3 [O60318-1]
UniGeneiHs.389037
Hs.592438

Genome annotation databases

EnsembliENST00000291688; ENSP00000291688; ENSG00000160294 [O60318-1]
ENST00000397708; ENSP00000380820; ENSG00000160294 [O60318-1]
GeneIDi8888
KEGGihsa:8888
UCSCiuc002zir.3 human [O60318-1]

Keywords - Coding sequence diversityi

Alternative promoter usage, Polymorphism

Similar proteinsi

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ010089 mRNA Translation: CAB52687.1
AY590469 Genomic DNA Translation: AAS89300.1
AP000471 Genomic DNA No translation available.
AP001469 Genomic DNA No translation available.
BC013285 mRNA Translation: AAH13285.2
BC004497 mRNA Translation: AAH04497.2
BC104958 mRNA Translation: AAI04959.1
BC104960 mRNA Translation: AAI04961.1
AB011144 mRNA Translation: BAA25498.1
AB005543 mRNA Translation: BAA25170.1
CCDSiCCDS13734.1 [O60318-1]
PIRiT00339
RefSeqiNP_003897.2, NM_003906.4 [O60318-1]
XP_005261260.1, XM_005261203.4 [O60318-1]
XP_005261261.1, XM_005261204.4 [O60318-1]
XP_005261262.1, XM_005261205.3 [O60318-1]
UniGeneiHs.389037
Hs.592438

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DHXX-ray2.10A/D1163-1235[»]
ProteinModelPortaliO60318
SMRiO60318
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114406, 61 interactors
DIPiDIP-31696N
IntActiO60318, 38 interactors
MINTiO60318
STRINGi9606.ENSP00000291688

Protein family/group databases

TCDBi1.I.1.1.3 the eukaryotic nuclear pore complex (e-npc) family

PTM databases

iPTMnetiO60318
PhosphoSitePlusiO60318

Polymorphism and mutation databases

BioMutaiMCM3AP

Proteomic databases

EPDiO60318
MaxQBiO60318
PaxDbiO60318
PeptideAtlasiO60318
PRIDEiO60318
ProteomicsDBi49343

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000291688; ENSP00000291688; ENSG00000160294 [O60318-1]
ENST00000397708; ENSP00000380820; ENSG00000160294 [O60318-1]
GeneIDi8888
KEGGihsa:8888
UCSCiuc002zir.3 human [O60318-1]

Organism-specific databases

CTDi8888
DisGeNETi8888
EuPathDBiHostDB:ENSG00000160294.10
GeneCardsiMCM3AP
H-InvDBiHIX0016189
HGNCiHGNC:6946 MCM3AP
HPAiHPA021527
HPA032103
MalaCardsiMCM3AP
MIMi603294 gene
neXtProtiNX_O60318
OpenTargetsiENSG00000160294
PharmGKBiPA30692
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1860 Eukaryota
COG5079 LUCA
GeneTreeiENSGT00530000063781
HOGENOMiHOG000113500
HOVERGENiHBG102020
InParanoidiO60318
OMAiNSSEVKF
OrthoDBiEOG091G01V4
PhylomeDBiO60318
TreeFamiTF105948

Miscellaneous databases

ChiTaRSiMCM3AP human
GeneWikiiMCM3AP
GenomeRNAii8888
PROiPR:O60318
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000160294 Expressed in 222 organ(s), highest expression level in tendon
CleanExiHS_MCM3AP
ExpressionAtlasiO60318 baseline and differential
GenevisibleiO60318 HS

Family and domain databases

CDDicd12443 RRM_MCM3A_like, 1 hit
InterProiView protein in InterPro
IPR031910 GANP_CID_dom
IPR031907 MCM3AP_GANP
IPR034265 MCM3AP_RRM
IPR031908 NupH_GANP
IPR000717 PCI_dom
IPR035979 RBD_domain_sf
IPR005062 SAC3/GANP/THP3
PfamiView protein in Pfam
PF16766 CID_GANP, 1 hit
PF16769 MCM3AP_GANP, 1 hit
PF16768 NupH_GANP, 1 hit
PF03399 SAC3_GANP, 1 hit
SUPFAMiSSF54928 SSF54928, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGANP_HUMAN
AccessioniPrimary (citable) accession number: O60318
Secondary accession number(s): C9JL56
, Q2M3C1, Q6PJP6, Q9BSY5, Q9UMT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: November 7, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 21
    Human chromosome 21: entries, gene names and cross-references to MIM
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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