UniProtKB - O60239 (3BP5_HUMAN)
SH3 domain-binding protein 5
SH3BP5
Functioni
Functions as guanine nucleotide exchange factor (GEF) with specificity for RAB11A and RAB25 (PubMed:26506309, PubMed:30217979).
Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death.
4 PublicationsGO - Molecular functioni
- guanyl-nucleotide exchange factor activity Source: UniProtKB
- protein kinase inhibitor activity Source: MGI
- SH3 domain binding Source: UniProtKB-KW
GO - Biological processi
- intracellular signal transduction Source: MGI
- negative regulation of protein tyrosine kinase activity Source: GO_Central
- signal transduction Source: ProtInc
Keywordsi
Molecular function | Guanine-nucleotide releasing factor |
Enzyme and pathway databases
PathwayCommonsi | O60239 |
SignaLinki | O60239 |
SIGNORi | O60239 |
Names & Taxonomyi
Protein namesi | Recommended name: SH3 domain-binding protein 5Short name: SH3BP-5 Alternative name(s): SH3 domain-binding protein that preferentially associates with BTK |
Gene namesi | Name:SH3BP5 Synonyms:SAB |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:10827, SH3BP5 |
MIMi | 605612, gene |
neXtProti | NX_O60239 |
VEuPathDBi | HostDB:ENSG00000131370 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Other locations
- Cytoplasmic vesicle membrane 1 Publication; Peripheral membrane protein 1 Publication
Note: Colocalizes with RAB11A on cytoplasmic vesicle membranes.1 Publication
Mitochondrion
- mitochondrion Source: UniProtKB-SubCell
Nucleus
- nuclear body Source: HPA
- nucleoplasm Source: HPA
Other locations
- cytoplasm Source: GO_Central
- cytoplasmic vesicle membrane Source: UniProtKB
Keywords - Cellular componenti
Cytoplasmic vesicle, Membrane, MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 52 – 54 | LNQ → AAA: Loss of guanine nucleotide exchange factor activity. 1 Publication | 3 | |
Mutagenesisi | 250 – 251 | LE → AK: Loss of guanine nucleotide exchange factor activity. 1 Publication | 2 | |
Mutagenesisi | 347 | L → A: Loss of phosphorylation and binding by phospho-JNK; when associated with A-349. 1 Publication | 1 | |
Mutagenesisi | 349 | L → A: Loss of phosphorylation and binding by phospho-JNK; when associated with A-347. 1 Publication | 1 | |
Mutagenesisi | 434 | L → A: No change of phosphorylation or binding by phospho-JNK; when associated with A-436. 1 Publication | 1 | |
Mutagenesisi | 436 | L → A: No change of phosphorylation or binding by phospho-JNK; when associated with A-434. 1 Publication | 1 |
Organism-specific databases
DisGeNETi | 9467 |
OpenTargetsi | ENSG00000131370 |
PharmGKBi | PA35735 |
Miscellaneous databases
Pharosi | O60239, Tbio |
Genetic variation databases
BioMutai | SH3BP5 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064368 | 1 – 455 | SH3 domain-binding protein 5Add BLAST | 455 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 351 | Phosphoserine; by MAPK12 and MAPK9By similarity | 1 | |
Modified residuei | 375 | PhosphoserineBy similarity | 1 | |
Modified residuei | 376 | PhosphoserineBy similarity | 1 | |
Modified residuei | 418 | PhosphoserineBy similarity | 1 | |
Modified residuei | 421 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O60239 |
jPOSTi | O60239 |
MassIVEi | O60239 |
MaxQBi | O60239 |
PaxDbi | O60239 |
PeptideAtlasi | O60239 |
PRIDEi | O60239 |
ProteomicsDBi | 49268 [O60239-1] 49269 [O60239-2] |
TopDownProteomicsi | O60239-1 [O60239-1] |
PTM databases
iPTMneti | O60239 |
PhosphoSitePlusi | O60239 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000131370, Expressed in metanephros and 250 other tissues |
ExpressionAtlasi | O60239, baseline and differential |
Genevisiblei | O60239, HS |
Organism-specific databases
HPAi | ENSG00000131370, Tissue enhanced (adrenal) |
Interactioni
Subunit structurei
Binary interactionsi
O60239
With | #Exp. | IntAct |
---|---|---|
BTK [Q06187] | 4 | EBI-624860,EBI-624835 |
IFT20 - isoform 3 [Q8IY31-3] | 3 | EBI-624860,EBI-9091197 |
NAP1L2 [Q9ULW6] | 3 | EBI-624860,EBI-3911716 |
GO - Molecular functioni
- SH3 domain binding Source: UniProtKB-KW
Protein-protein interaction databases
BioGRIDi | 114853, 23 interactors |
ELMi | O60239 |
IntActi | O60239, 22 interactors |
STRINGi | 9606.ENSP00000373301 |
Miscellaneous databases
RNActi | O60239, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | O60239 |
SMRi | O60239 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 63 | DisorderedSequence analysisAdd BLAST | 63 | |
Regioni | 31 – 265 | Sufficient for interaction with RAB11A and for guanine nucleotide exchange activity1 PublicationAdd BLAST | 235 | |
Regioni | 259 – 293 | DisorderedSequence analysisAdd BLAST | 35 | |
Regioni | 306 – 345 | DisorderedSequence analysisAdd BLAST | 40 | |
Regioni | 369 – 435 | DisorderedSequence analysisAdd BLAST | 67 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 44 – 90 | 1 PublicationAdd BLAST | 47 | |
Coiled coili | 97 – 145 | 1 PublicationAdd BLAST | 49 | |
Coiled coili | 154 – 200 | 1 PublicationAdd BLAST | 47 | |
Coiled coili | 211 – 255 | 1 PublicationAdd BLAST | 45 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 1 – 17 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 18 – 43 | Acidic residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 306 – 333 | Polar residuesSequence analysisAdd BLAST | 28 | |
Compositional biasi | 379 – 395 | Basic and acidic residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 396 – 435 | Polar residuesSequence analysisAdd BLAST | 40 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Coiled coil, SH3-bindingPhylogenomic databases
eggNOGi | KOG2008, Eukaryota |
GeneTreei | ENSGT00390000018500 |
HOGENOMi | CLU_048895_1_0_1 |
InParanoidi | O60239 |
OMAi | QFPPATR |
PhylomeDBi | O60239 |
TreeFami | TF105573 |
Family and domain databases
DisProti | DP02850 |
IDEALi | IID00507 |
InterProi | View protein in InterPro IPR007940, SH3BP5 |
PANTHERi | PTHR19423, PTHR19423, 2 hits |
Pfami | View protein in Pfam PF05276, SH3BP5, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MDAALKRSRS EEPAEILPPA RDEEEEEEEG MEQGLEEEEE VDPRIQGELE
60 70 80 90 100
KLNQSTDDIN RRETELEDAR QKFRSVLVEA TVKLDELVKK IGKAVEDSKP
110 120 130 140 150
YWEARRVARQ AQLEAQKATQ DFQRATEVLR AAKETISLAE QRLLEDDKRQ
160 170 180 190 200
FDSAWQEMLN HATQRVMEAE QTKTRSELVH KETAARYNAA MGRMRQLEKK
210 220 230 240 250
LKRAINKSKP YFELKAKYYV QLEQLKKTVD DLQAKLTLAK GEYKMALKNL
260 270 280 290 300
EMISDEIHER RRSSAMGPRG CGVGAEGSST SVEDLPGSKP EPDAISVASE
310 320 330 340 350
AFEDDSCSNF VSEDDSETQS VSSFSSGPTS PSEMPDQFPA VVRPGSLDLP
360 370 380 390 400
SPVSLSEFGM MFPVLGPRSE CSGASSPECE VERGDRAEGA ENKTSDKANN
410 420 430 440 450
NRGLSSSSGS GGSSKSQSST SPEGQALENR MKQLSLQCSK GRDGIIADIK
MVQIG
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketC9JK30 | C9JK30_HUMAN | SH3 domain-binding protein 5 | SH3BP5 | 128 | Annotation score: | ||
F8WDU8 | F8WDU8_HUMAN | SH3 domain-binding protein 5 | SH3BP5 | 69 | Annotation score: | ||
C9JNW0 | C9JNW0_HUMAN | SH3 domain-binding protein 5 | SH3BP5 | 51 | Annotation score: | ||
C9J140 | C9J140_HUMAN | SH3 domain-binding protein 5 | SH3BP5 | 57 | Annotation score: |
Sequence cautioni
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_042854 | 1 – 157 | Missing in isoform 2. 1 PublicationAdd BLAST | 157 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK090524 mRNA Translation: BAG52178.1 AC087590 Genomic DNA No translation available. AL117422 mRNA Translation: CAI46220.1 CH471055 Genomic DNA Translation: EAW64225.1 BC010123 mRNA Translation: AAH10123.2 AB005047 mRNA Translation: BAA25922.1 Different initiation. |
CCDSi | CCDS2625.2 [O60239-1] CCDS43055.1 [O60239-2] |
PIRi | JE0086 |
RefSeqi | NP_001018009.2, NM_001018009.3 [O60239-2] NP_004835.2, NM_004844.4 [O60239-1] XP_016863011.1, XM_017007522.1 [O60239-2] XP_016863012.1, XM_017007523.1 [O60239-2] XP_016863013.1, XM_017007524.1 [O60239-2] XP_016863014.1, XM_017007525.1 [O60239-2] |
Genome annotation databases
Ensembli | ENST00000383791.8; ENSP00000373301.3; ENSG00000131370.16 ENST00000408919.7; ENSP00000386231.3; ENSG00000131370.16 [O60239-2] ENST00000426925.5; ENSP00000388553.1; ENSG00000131370.16 [O60239-2] |
GeneIDi | 9467 |
KEGGi | hsa:9467 |
MANE-Selecti | ENST00000383791.8; ENSP00000373301.3; NM_004844.5; NP_004835.2 |
UCSCi | uc003bzp.3, human [O60239-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK090524 mRNA Translation: BAG52178.1 AC087590 Genomic DNA No translation available. AL117422 mRNA Translation: CAI46220.1 CH471055 Genomic DNA Translation: EAW64225.1 BC010123 mRNA Translation: AAH10123.2 AB005047 mRNA Translation: BAA25922.1 Different initiation. |
CCDSi | CCDS2625.2 [O60239-1] CCDS43055.1 [O60239-2] |
PIRi | JE0086 |
RefSeqi | NP_001018009.2, NM_001018009.3 [O60239-2] NP_004835.2, NM_004844.4 [O60239-1] XP_016863011.1, XM_017007522.1 [O60239-2] XP_016863012.1, XM_017007523.1 [O60239-2] XP_016863013.1, XM_017007524.1 [O60239-2] XP_016863014.1, XM_017007525.1 [O60239-2] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4H3B | X-ray | 2.08 | B/D | 341-350 | [»] | |
6DJL | X-ray | 3.10 | B/C/D/E | 1-265 | [»] | |
6IXE | X-ray | 3.35 | A | 41-266 | [»] | |
6IXF | X-ray | 3.60 | A/B | 41-266 | [»] | |
6IXG | X-ray | 3.80 | A/B | 41-266 | [»] | |
6IXV | X-ray | 3.80 | A/B/C/D | 10-276 | [»] | |
AlphaFoldDBi | O60239 | |||||
SMRi | O60239 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 114853, 23 interactors |
ELMi | O60239 |
IntActi | O60239, 22 interactors |
STRINGi | 9606.ENSP00000373301 |
PTM databases
iPTMneti | O60239 |
PhosphoSitePlusi | O60239 |
Genetic variation databases
BioMutai | SH3BP5 |
Proteomic databases
EPDi | O60239 |
jPOSTi | O60239 |
MassIVEi | O60239 |
MaxQBi | O60239 |
PaxDbi | O60239 |
PeptideAtlasi | O60239 |
PRIDEi | O60239 |
ProteomicsDBi | 49268 [O60239-1] 49269 [O60239-2] |
TopDownProteomicsi | O60239-1 [O60239-1] |
Protocols and materials databases
Antibodypediai | 26718, 165 antibodies from 27 providers |
DNASUi | 9467 |
Genome annotation databases
Ensembli | ENST00000383791.8; ENSP00000373301.3; ENSG00000131370.16 ENST00000408919.7; ENSP00000386231.3; ENSG00000131370.16 [O60239-2] ENST00000426925.5; ENSP00000388553.1; ENSG00000131370.16 [O60239-2] |
GeneIDi | 9467 |
KEGGi | hsa:9467 |
MANE-Selecti | ENST00000383791.8; ENSP00000373301.3; NM_004844.5; NP_004835.2 |
UCSCi | uc003bzp.3, human [O60239-1] |
Organism-specific databases
CTDi | 9467 |
DisGeNETi | 9467 |
GeneCardsi | SH3BP5 |
HGNCi | HGNC:10827, SH3BP5 |
HPAi | ENSG00000131370, Tissue enhanced (adrenal) |
MIMi | 605612, gene |
neXtProti | NX_O60239 |
OpenTargetsi | ENSG00000131370 |
PharmGKBi | PA35735 |
VEuPathDBi | HostDB:ENSG00000131370 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG2008, Eukaryota |
GeneTreei | ENSGT00390000018500 |
HOGENOMi | CLU_048895_1_0_1 |
InParanoidi | O60239 |
OMAi | QFPPATR |
PhylomeDBi | O60239 |
TreeFami | TF105573 |
Enzyme and pathway databases
PathwayCommonsi | O60239 |
SignaLinki | O60239 |
SIGNORi | O60239 |
Miscellaneous databases
BioGRID-ORCSi | 9467, 16 hits in 1081 CRISPR screens |
ChiTaRSi | SH3BP5, human |
GeneWikii | SH3BP5 |
GenomeRNAii | 9467 |
Pharosi | O60239, Tbio |
PROi | PR:O60239 |
RNActi | O60239, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000131370, Expressed in metanephros and 250 other tissues |
ExpressionAtlasi | O60239, baseline and differential |
Genevisiblei | O60239, HS |
Family and domain databases
DisProti | DP02850 |
IDEALi | IID00507 |
InterProi | View protein in InterPro IPR007940, SH3BP5 |
PANTHERi | PTHR19423, PTHR19423, 2 hits |
Pfami | View protein in Pfam PF05276, SH3BP5, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | 3BP5_HUMAN | |
Accessioni | O60239Primary (citable) accession number: O60239 Secondary accession number(s): B3KQW6, Q5JWV9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2002 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 176 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families