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Protein

Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16

Gene

DHX16

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in pre-mRNA splicing. Contributes to pre-mRNA splicing after spliceosome formation and prior to the first transesterification reaction.3 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi422 – 429ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATPase activity Source: Reactome
  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • RNA binding Source: UniProtKB
  • RNA helicase activity Source: ProtInc

GO - Biological processi

  • mRNA splicing, via spliceosome Source: UniProtKB
  • RNA splicing Source: ProtInc

Keywordsi

Molecular functionHelicase, Hydrolase
Biological processmRNA processing, mRNA splicing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.4.13 2681
ReactomeiR-HSA-72163 mRNA Splicing - Major Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (EC:3.6.4.13)
Alternative name(s):
ATP-dependent RNA helicase #3
DEAH-box protein 16
Gene namesi
Name:DHX16
Synonyms:DBP2, DDX16, KIAA0577, PRP22 Publications
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000204560.9
HGNCiHGNC:2739 DHX16
MIMi603405 gene
neXtProtiNX_O60231

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi428K → A: Impairs pre-mRNA splicing activity. 1 Publication1
Mutagenesisi520D → A: Impairs pre-mRNA splicing activity. 1 Publication1
Mutagenesisi523H → A: No loss of pre-mRNA splicing activity. 1 Publication1
Mutagenesisi552S → L: Dominant-negative mutant. Impairs pre-mRNA splicing activity. 2 Publications1
Mutagenesisi724G → N: Dominant-negative mutant. Impairs pre-mRNA splicing activity. 3 Publications1

Organism-specific databases

DisGeNETi8449
OpenTargetsiENSG00000204560
PharmGKBiPA27205

Polymorphism and mutation databases

BioMutaiDHX16

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000551511 – 1041Pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16Add BLAST1041

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei103PhosphoserineCombined sources1
Modified residuei106PhosphoserineCombined sources1
Modified residuei107PhosphoserineCombined sources1
Modified residuei160PhosphoserineCombined sources1
Modified residuei712PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO60231
PaxDbiO60231
PeptideAtlasiO60231
PRIDEiO60231
ProteomicsDBi49258

PTM databases

iPTMnetiO60231
PhosphoSitePlusiO60231

Expressioni

Gene expression databases

BgeeiENSG00000204560
CleanExiHS_DHX16
ExpressionAtlasiO60231 baseline and differential
GenevisibleiO60231 HS

Organism-specific databases

HPAiHPA051455

Interactioni

Subunit structurei

Interacts with GPKOW.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi114027, 62 interactors
CORUMiO60231
IntActiO60231, 22 interactors
MINTiO60231
STRINGi9606.ENSP00000365625

Structurei

3D structure databases

ProteinModelPortaliO60231
SMRiO60231
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini409 – 573Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST165
Domaini598 – 771Helicase C-terminalPROSITE-ProRule annotationAdd BLAST174

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi520 – 523DEAH box4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0925 Eukaryota
COG1643 LUCA
GeneTreeiENSGT00910000144017
HOGENOMiHOG000175261
HOVERGENiHBG039428
InParanoidiO60231
KOiK12813
OMAiVYNQWVE
OrthoDBiEOG0937063J
PhylomeDBiO60231
TreeFamiTF313473

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR002464 DNA/RNA_helicase_DEAH_CS
IPR011709 DUF1605
IPR007502 Helicase-assoc_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF04408 HA2, 1 hit
PF00271 Helicase_C, 1 hit
PF07717 OB_NTP_bind, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00847 HA2, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00690 DEAH_ATP_HELICASE, 1 hit
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

O60231-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATPAGLERW VQDELHSVLG LSERHVAQFL IGTAQRCTSA EEFVQRLRDT
60 70 80 90 100
DTLDLSGPAR DFALRLWNKV PRKAVVEKPA RAAEREARAL LEKNRSYRLL
110 120 130 140 150
EDSEESSEET VSRAGSSLQK KRKKRKHLRK KREEEEEEEA SEKGKKKTGG
160 170 180 190 200
SKQQTEKPES EDEWERTERE RLQDLEERDA FAERVRQRDK DRTRNVLERS
210 220 230 240 250
DKKAYEEAQK RLKMAEEDRK AMVPELRKKS RREYLAKRER EKLEDLEAEL
260 270 280 290 300
ADEEFLFGDV ELSRHERQEL KYKRRVRDLA REYRAAGEQE KLEATNRYHM
310 320 330 340 350
PKETRGQPAR AVDLVEEESG APGEEQRRWE EARLGAASLK FGARDAASQE
360 370 380 390 400
PKYQLVLEEE ETIEFVRATQ LQGDEEPSAP PTSTQAQQKE SIQAVRRSLP
410 420 430 440 450
VFPFREELLA AIANHQVLII EGETGSGKTT QIPQYLFEEG YTNKGMKIAC
460 470 480 490 500
TQPRRVAAMS VAARVAREMG VKLGNEVGYS IRFEDCTSER TVLRYMTDGM
510 520 530 540 550
LLREFLSEPD LASYSVVMVD EAHERTLHTD ILFGLIKDVA RFRPELKVLV
560 570 580 590 600
ASATMDTARF STFFDDAPVF RIPGRRFPVD IFYTKAPEAD YLEACVVSVL
610 620 630 640 650
QIHVTQPPGD ILVFLTGQEE IEAACEMLQD RCRRLGSKIR ELLVLPIYAN
660 670 680 690 700
LPSDMQARIF QPTPPGARKV VVATNIAETS LTIEGIIYVL DPGFCKQKSY
710 720 730 740 750
NPRTGMESLT VTPCSKASAN QRAGRAGRVA AGKCFRLYTA WAYQHELEET
760 770 780 790 800
TVPEIQRTSL GNVVLLLKSL GIHDLMHFDF LDPPPYETLL LALEQLYALG
810 820 830 840 850
ALNHLGELTT SGRKMAELPV DPMLSKMILA SEKYSCSEEI LTVAAMLSVN
860 870 880 890 900
NSIFYRPKDK VVHADNARVN FFLPGGDHLV LLNVYTQWAE SGYSSQWCYE
910 920 930 940 950
NFVQFRSMRR ARDVREQLEG LLERVEVGLS SCQGDYIRVR KAITAGYFYH
960 970 980 990 1000
TARLTRSGYR TVKQQQTVFI HPNSSLFEQQ PRWLLYHELV LTTKEFMRQV
1010 1020 1030 1040
LEIESSWLLE VAPHYYKAKE LEDPHAKKMP KKIGKTREEL G
Length:1,041
Mass (Da):119,264
Last modified:November 28, 2002 - v2
Checksum:i5CAC4DBEF21A4E6D
GO

Sequence cautioni

The sequence BAA25503 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti223V → I in BAA25908 (PubMed:9547260).Curated1
Sequence conflicti631R → P in BAA25908 (PubMed:9547260).Curated1
Sequence conflicti681L → P in BAA25908 (PubMed:9547260).Curated1
Sequence conflicti792A → T in BAA25908 (PubMed:9547260).Curated1
Sequence conflicti899Y → C in BAA25908 (PubMed:9547260).Curated1
Sequence conflicti1032K → KK in AAH08825 (PubMed:15489334).Curated1
Sequence conflicti1032K → KK in AAH09392 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_057236352K → E. Corresponds to variant dbSNP:rs17189239Ensembl.1
Natural variantiVAR_057237502L → F. Corresponds to variant dbSNP:rs17189232Ensembl.1
Natural variantiVAR_057238566D → G1 PublicationCorresponds to variant dbSNP:rs9262138Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB001601 mRNA Translation: BAA25908.1
BA000025 Genomic DNA Translation: BAB63323.1
AB011149 mRNA Translation: BAA25503.2 Different initiation.
AL845353 Genomic DNA No translation available.
BC008825 mRNA Translation: AAH08825.1
BC009392 mRNA Translation: AAH09392.1
CCDSiCCDS4685.1
RefSeqiNP_001157711.1, NM_001164239.1
NP_003578.2, NM_003587.4
UniGeneiHs.485060

Genome annotation databases

EnsembliENST00000376442; ENSP00000365625; ENSG00000204560
ENST00000383577; ENSP00000373071; ENSG00000206486
ENST00000417308; ENSP00000390938; ENSG00000233561
ENST00000421095; ENSP00000396193; ENSG00000226171
ENST00000424672; ENSP00000389862; ENSG00000233418
ENST00000451456; ENSP00000408956; ENSG00000233049
ENST00000458094; ENSP00000393958; ENSG00000231377
GeneIDi8449
KEGGihsa:8449
UCSCiuc011ijv.3 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiDHX16_HUMAN
AccessioniPrimary (citable) accession number: O60231
Secondary accession number(s): O60322
, Q5JP45, Q969X7, Q96QC1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: November 28, 2002
Last modified: June 20, 2018
This is version 189 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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