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Protein

Aldo-keto reductase family 1 member B10

Gene

AKR1B10

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs.1 Publication

Enzyme regulationi

Inhibited by tolrestat.1 Publication

Kineticsi

  1. KM=600 µM for D,L-glyceraldehyde1 Publication
  2. KM=0.6 µM for all-trans-retinaldehyde1 Publication
  3. KM=0.7 µM for 9-cis-retinaldehyde1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei44NADP1 Publication1
    Active sitei49Proton donor1 Publication1
    Sitei78Lowers pKa of active site TyrBy similarity1
    Binding sitei111Substrate1 Publication1
    Binding sitei184NADP1 Publication1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi20 – 22NADP1 Publication3
    Nucleotide bindingi160 – 161NADP1 Publication2
    Nucleotide bindingi210 – 217NADP1 Publication8
    Nucleotide bindingi261 – 273NADP1 PublicationAdd BLAST13

    GO - Molecular functioni

    • aldo-keto reductase (NADP) activity Source: UniProtKB
    • geranylgeranyl reductase activity Source: UniProtKB
    • indanol dehydrogenase activity Source: UniProtKB
    • retinal dehydrogenase activity Source: UniProtKB

    GO - Biological processi

    • daunorubicin metabolic process Source: UniProtKB
    • doxorubicin metabolic process Source: UniProtKB
    • farnesol catabolic process Source: UniProtKB
    • retinoid metabolic process Source: Reactome

    Keywordsi

    Molecular functionOxidoreductase
    LigandNADP

    Enzyme and pathway databases

    BRENDAi1.1.1.21 2681
    ReactomeiR-HSA-975634 Retinoid metabolism and transport
    SABIO-RKiO60218

    Chemistry databases

    SwissLipidsiSLP:000001873

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aldo-keto reductase family 1 member B10 (EC:1.1.1.-)
    Alternative name(s):
    ARL-1
    Aldose reductase-like
    Aldose reductase-related protein
    Short name:
    ARP
    Short name:
    hARP
    Small intestine reductase
    Short name:
    SI reductase
    Gene namesi
    Name:AKR1B10
    Synonyms:AKR1B11
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 7

    Organism-specific databases

    EuPathDBiHostDB:ENSG00000198074.9
    HGNCiHGNC:382 AKR1B10
    MIMi604707 gene
    neXtProtiNX_O60218

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Lysosome, Secreted

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi125K → L: Increased affinity and reduced catalytic activity towards all-trans-retinaldehyde. 1 Publication1
    Mutagenesisi301V → L: Reduced catalytic activity towards all-trans-retinaldehyde. 1 Publication1

    Organism-specific databases

    DisGeNETi57016
    OpenTargetsiENSG00000198074
    PharmGKBiPA24676

    Chemistry databases

    ChEMBLiCHEMBL5983
    DrugBankiDB03461 2'-Monophosphoadenosine 5'-Diphosphoribose
    DB02383 Tolrestat

    Polymorphism and mutation databases

    BioMutaiAKR1B10

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001246321 – 316Aldo-keto reductase family 1 member B10Add BLAST316

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei125N6-acetyllysineCombined sources1
    Modified residuei263N6-acetyllysineCombined sources1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    EPDiO60218
    MaxQBiO60218
    PaxDbiO60218
    PeptideAtlasiO60218
    PRIDEiO60218
    ProteomicsDBi49247

    2D gel databases

    DOSAC-COBS-2DPAGEiO60218

    PTM databases

    iPTMnetiO60218
    PhosphoSitePlusiO60218

    Expressioni

    Tissue specificityi

    Found in many tissues. Highly expressed in small intestine, colon and adrenal gland.

    Gene expression databases

    BgeeiENSG00000198074
    CleanExiHS_AKR1B10
    GenevisibleiO60218 HS

    Organism-specific databases

    HPAiCAB070163
    HPA020280
    HPA073633

    Interactioni

    Binary interactionsi

    Show more details

    Protein-protein interaction databases

    BioGridi121325, 18 interactors
    IntActiO60218, 6 interactors
    STRINGi9606.ENSP00000352584

    Chemistry databases

    BindingDBiO60218

    Structurei

    Secondary structure

    1316
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi4 – 6Combined sources3
    Beta strandi12 – 19Combined sources8
    Turni25 – 27Combined sources3
    Helixi28 – 37Combined sources10
    Beta strandi42 – 44Combined sources3
    Helixi47 – 49Combined sources3
    Helixi52 – 64Combined sources13
    Helixi70 – 72Combined sources3
    Beta strandi74 – 79Combined sources6
    Helixi81 – 83Combined sources3
    Helixi86 – 100Combined sources15
    Beta strandi105 – 111Combined sources7
    Beta strandi118 – 120Combined sources3
    Beta strandi127 – 131Combined sources5
    Helixi138 – 150Combined sources13
    Beta strandi153 – 161Combined sources9
    Helixi164 – 171Combined sources8
    Beta strandi181 – 186Combined sources6
    Helixi194 – 202Combined sources9
    Beta strandi206 – 211Combined sources6
    Helixi228 – 230Combined sources3
    Helixi232 – 240Combined sources9
    Helixi245 – 254Combined sources10
    Turni255 – 257Combined sources3
    Helixi267 – 274Combined sources8
    Helixi283 – 290Combined sources8
    Helixi302 – 304Combined sources3
    Beta strandi305 – 307Combined sources3
    Helixi311 – 313Combined sources3

    3D structure databases

    ProteinModelPortaliO60218
    SMRiO60218
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiO60218

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the aldo/keto reductase family.Curated

    Phylogenomic databases

    eggNOGiKOG1577 Eukaryota
    COG0656 LUCA
    GeneTreeiENSGT00760000119041
    HOGENOMiHOG000250272
    HOVERGENiHBG000020
    InParanoidiO60218
    KOiK00011
    OMAiWLVHWPP
    OrthoDBiEOG091G0D69
    PhylomeDBiO60218
    TreeFamiTF106492

    Family and domain databases

    CDDicd06660 Aldo_ket_red, 1 hit
    Gene3Di3.20.20.100, 1 hit
    InterProiView protein in InterPro
    IPR018170 Aldo/ket_reductase_CS
    IPR020471 Aldo/keto_reductase
    IPR023210 NADP_OxRdtase_dom
    IPR036812 NADP_OxRdtase_dom_sf
    PANTHERiPTHR11732 PTHR11732, 1 hit
    PfamiView protein in Pfam
    PF00248 Aldo_ket_red, 1 hit
    PIRSFiPIRSF000097 AKR, 1 hit
    PRINTSiPR00069 ALDKETRDTASE
    SUPFAMiSSF51430 SSF51430, 1 hit
    PROSITEiView protein in PROSITE
    PS00798 ALDOKETO_REDUCTASE_1, 1 hit
    PS00062 ALDOKETO_REDUCTASE_2, 1 hit
    PS00063 ALDOKETO_REDUCTASE_3, 1 hit

    Sequencei

    Sequence statusi: Complete.

    O60218-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ
    60 70 80 90 100
    NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL
    110 120 130 140 150
    KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD
    160 170 180 190 200
    EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC
    210 220 230 240 250
    HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI
    260 270 280 290 300
    RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN
    310
    VLQSSHLEDY PFNAEY
    Length:316
    Mass (Da):36,020
    Last modified:February 8, 2011 - v2
    Checksum:i0C2FC0E798955A33
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti76V → A in AAO13380 (Ref. 3) Curated1
    Sequence conflicti100L → P in AAO13380 (Ref. 3) Curated1
    Sequence conflicti288T → I in AAO13380 (Ref. 3) Curated1

    Natural variant

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Natural variantiVAR_02007787P → S. Corresponds to variant dbSNP:rs2303312Ensembl.1
    Natural variantiVAR_020078286M → T. Corresponds to variant dbSNP:rs3735042Ensembl.1
    Natural variantiVAR_013287313N → DCombined sources6 PublicationsCorresponds to variant dbSNP:rs4728329Ensembl.1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    U37100 mRNA Translation: AAC17469.1
    AF052577 mRNA Translation: AAC36465.1
    AF524864 mRNA Translation: AAO13380.1
    BT006794 mRNA Translation: AAP35440.1
    CR541801 mRNA Translation: CAG46600.1
    AC078847 Genomic DNA No translation available.
    CH236950 Genomic DNA Translation: EAL24069.1
    CH471070 Genomic DNA Translation: EAW83816.1
    BC008837 mRNA Translation: AAH08837.1
    AF044961 mRNA Translation: AAC15671.1
    CCDSiCCDS5832.1
    RefSeqiNP_064695.3, NM_020299.4
    UniGeneiHs.116724

    Genome annotation databases

    EnsembliENST00000359579; ENSP00000352584; ENSG00000198074
    GeneIDi57016
    KEGGihsa:57016
    UCSCiuc003vrr.4 human

    Keywords - Coding sequence diversityi

    Polymorphism

    Similar proteinsi

    Entry informationi

    Entry nameiAK1BA_HUMAN
    AccessioniPrimary (citable) accession number: O60218
    Secondary accession number(s): A4D1P1
    , O75890, Q6FHF3, Q8IWZ1
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2002
    Last sequence update: February 8, 2011
    Last modified: July 18, 2018
    This is version 166 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    6. SIMILARITY comments
      Index of protein domains and families

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