UniProtKB - O60200 (MDM35_YEAST)
Mitochondrial distribution and morphology protein 35
MDM35
Functioni
Involved in mitochondrial distribution and morphology. Mediates the import of UPS1, UPS2 and UPS3, 3 atypical mitochondrial intermembrane space (IMS) proteins lacking the two major IMS-targeting signals, into the intermembrane space. The UPS1:MDM35 complex mediates the transfer of phosphatidic acid (PA) between liposomes and probably functions as a PA transporter across the mitochondrion intermembrane space (PubMed:26071602, PubMed:26071601, PubMed:26235513).
Phosphatidic acid import is required for cardiolipin (CL) synthesis in the mitochondrial inner membrane (PubMed:26071602).
6 PublicationsMiscellaneous
GO - Biological processi
- mitochondrial respiratory chain complex assembly Source: SGD
- mitochondrion organization Source: SGD
- phospholipid translocation Source: SGD
- phospholipid transport Source: SGD
Keywordsi
Biological process | Lipid transport, Transport |
Enzyme and pathway databases
Reactomei | R-SCE-6803204, TP53 Regulates Transcription of Genes Involved in Cytochrome C Release |
Names & Taxonomyi
Protein namesi | Recommended name: Mitochondrial distribution and morphology protein 35 |
Gene namesi | Name:MDM35 Ordered Locus Names:YKL053C-A |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000007243, MDM35 |
VEuPathDBi | FungiDB:YKL053C-A |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Mitochondrion
- Mitochondrion intermembrane space 4 Publications
Mitochondrion
- mitochondrial intermembrane space Source: SGD
- mitochondrion Source: SGD
Nucleus
- nucleus Source: SGD
Other locations
- cytoplasm Source: SGD
Keywords - Cellular componenti
Cytoplasm, Mitochondrion, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 24 | F → A: Impairs interaction with UPS1 and UPS2; when associated with A-27 and A-28. 1 Publication | 1 | |
Mutagenesisi | 27 | W → A: Impairs interaction with UPS1 and UPS2; when associated with A-24 and A-28. 1 Publication | 1 | |
Mutagenesisi | 28 | Y → A: Impairs interaction with UPS1 and UPS2; when associated with A-24 and A-27. 1 Publication | 1 | |
Mutagenesisi | 32 | F → A: Impairs interaction with UPS1 and UPS2. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000220526 | 1 – 86 | Mitochondrial distribution and morphology protein 35Add BLAST | 86 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 13 ↔ 52 | PROSITE-ProRule annotationCombined sources2 Publications | ||
Disulfide bondi | 23 ↔ 42 | PROSITE-ProRule annotationCombined sources2 Publications |
Keywords - PTMi
Disulfide bondProteomic databases
MaxQBi | O60200 |
PaxDbi | O60200 |
PRIDEi | O60200 |
Interactioni
Subunit structurei
Interacts with UPS1, UPS2 and UPS3.
4 PublicationsBinary interactionsi
O60200
With | #Exp. | IntAct |
---|---|---|
UPS1 [Q05776] | 5 | EBI-2080774,EBI-30337 |
UPS2 [P35200] | 5 | EBI-2080774,EBI-11337 |
UPS3 [Q04006] | 3 | EBI-2080774,EBI-3830982 |
Protein-protein interaction databases
BioGRIDi | 34080, 405 interactors |
IntActi | O60200, 3 interactors |
MINTi | O60200 |
STRINGi | 4932.YKL053C-A |
Miscellaneous databases
RNActi | O60200, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | O60200 |
SMRi | O60200 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 60 | CHCHPROSITE-ProRule annotationAdd BLAST | 51 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 13 – 23 | Cx9C motif 1PROSITE-ProRule annotationAdd BLAST | 11 | |
Motifi | 42 – 52 | Cx9C motif 2PROSITE-ProRule annotationAdd BLAST | 11 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3481, Eukaryota |
GeneTreei | ENSGT00940000176689 |
HOGENOMi | CLU_101473_2_0_1 |
InParanoidi | O60200 |
OMAi | KQWYAYT |
Family and domain databases
DisProti | DP02325 |
InterProi | View protein in InterPro IPR007918, MDM35_apoptosis |
PANTHERi | PTHR46403, PTHR46403, 1 hit |
Pfami | View protein in Pfam PF05254, UPF0203, 1 hit |
PROSITEi | View protein in PROSITE PS51808, CHCH, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MGNIMSASFA PECTDLKTKY DSCFNEWYSE KFLKGKSVEN ECSKQWYAYT
60 70 80
TCVNAALVKQ GIKPALDEAR EEAPFENGGK LKEVDK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z28054 Genomic DNA Translation: CAA81891.1 Z28052 Genomic DNA Translation: CAA81889.1 AY558262 Genomic DNA Translation: AAS56588.1 BK006944 Genomic DNA Translation: DAA09104.1 |
RefSeqi | NP_012870.1, NM_001184336.1 |
Genome annotation databases
EnsemblFungii | YKL053C-A_mRNA; YKL053C-A; YKL053C-A |
GeneIDi | 853812 |
KEGGi | sce:YKL053C-A |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | Z28054 Genomic DNA Translation: CAA81891.1 Z28052 Genomic DNA Translation: CAA81889.1 AY558262 Genomic DNA Translation: AAS56588.1 BK006944 Genomic DNA Translation: DAA09104.1 |
RefSeqi | NP_012870.1, NM_001184336.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4XHR | X-ray | 2.55 | M/N | 1-86 | [»] | |
4XIZ | X-ray | 2.00 | M/N | 6-75 | [»] | |
4YTV | X-ray | 1.45 | A | 1-81 | [»] | |
4YTW | X-ray | 1.40 | A/C | 1-81 | [»] | |
4YTX | X-ray | 3.20 | A/C/E/G/I/K/M/O | 1-81 | [»] | |
5JQL | X-ray | 2.90 | B/D/F/H/J/L | 1-86 | [»] | |
5JQM | X-ray | 1.50 | A/B/C | 1-86 | [»] | |
6KYL | X-ray | 3.55 | A/C | 1-86 | [»] | |
AlphaFoldDBi | O60200 | |||||
SMRi | O60200 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 34080, 405 interactors |
IntActi | O60200, 3 interactors |
MINTi | O60200 |
STRINGi | 4932.YKL053C-A |
Proteomic databases
MaxQBi | O60200 |
PaxDbi | O60200 |
PRIDEi | O60200 |
Genome annotation databases
EnsemblFungii | YKL053C-A_mRNA; YKL053C-A; YKL053C-A |
GeneIDi | 853812 |
KEGGi | sce:YKL053C-A |
Organism-specific databases
SGDi | S000007243, MDM35 |
VEuPathDBi | FungiDB:YKL053C-A |
Phylogenomic databases
eggNOGi | KOG3481, Eukaryota |
GeneTreei | ENSGT00940000176689 |
HOGENOMi | CLU_101473_2_0_1 |
InParanoidi | O60200 |
OMAi | KQWYAYT |
Enzyme and pathway databases
Reactomei | R-SCE-6803204, TP53 Regulates Transcription of Genes Involved in Cytochrome C Release |
Miscellaneous databases
PROi | PR:O60200 |
RNActi | O60200, protein |
Family and domain databases
DisProti | DP02325 |
InterProi | View protein in InterPro IPR007918, MDM35_apoptosis |
PANTHERi | PTHR46403, PTHR46403, 1 hit |
Pfami | View protein in Pfam PF05254, UPF0203, 1 hit |
PROSITEi | View protein in PROSITE PS51808, CHCH, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MDM35_YEAST | |
Accessioni | O60200Primary (citable) accession number: O60200 Secondary accession number(s): D6VXN4, Q6Q599 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 1, 2000 |
Last sequence update: | April 12, 2005 | |
Last modified: | May 25, 2022 | |
This is version 159 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome XI
Yeast (Saccharomyces cerevisiae) chromosome XI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families