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Protein

Mitochondrial import inner membrane translocase subunit tim44

Gene

tim44

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Recruits mitochondrial HSP70 and its co-chaperone (mge1) to drive protein translocation into the matrix using ATP as an energy source.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi90 – 97ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chaperone binding Source: GO_Central

GO - Biological processi

Keywordsi

Biological processProtein transport, Translocation, Transport
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import inner membrane translocase subunit tim44
Gene namesi
Name:tim44
ORF Names:SPBC14C8.02
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC14C8.02
PomBaseiSPBC14C8.02 tim44

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000034318? – 427Mitochondrial import inner membrane translocase subunit tim44
Transit peptidei1 – ?MitochondrionSequence analysis

Proteomic databases

MaxQBiO60084
PaxDbiO60084
PRIDEiO60084

Interactioni

Subunit structurei

Heterodimer with pam18. Component of the PAM complex, at least composed of mtHsp70, mge1, tim44, pam16, pam17 and pam18. The complex interacts with the tim23 component of the PAM complex.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiO60084, 2 interactors
STRINGi4896.SPBC14C8.02.1

Structurei

3D structure databases

ProteinModelPortaliO60084
SMRiO60084
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Tim44 family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000208172
InParanoidiO60084
KOiK17804
OMAiRFLKQCE
OrthoDBiEOG092C2GAN
PhylomeDBiO60084

Family and domain databases

InterProiView protein in InterPro
IPR032710 NTF2-like_dom_sf
IPR017303 Tim44
IPR039544 Tim44-like
IPR007379 Tim44-like_dom
PANTHERiPTHR10721 PTHR10721, 1 hit
PfamiView protein in Pfam
PF04280 Tim44, 1 hit
PIRSFiPIRSF037871 TIM44, 1 hit
SMARTiView protein in SMART
SM00978 Tim44, 1 hit
SUPFAMiSSF54427 SSF54427, 1 hit
TIGRFAMsiTIGR00984 3a0801s03tim44, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O60084-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFLSRKITKN ARTIVQCQRF FQTSIFSQNA APQSPIKVFM DTFRAELKKS
60 70 80 90 100
QELQDSVKAL QDSSGSLSES DTFKKARDAY EKARSGTTAA SSFTGKTVGK
110 120 130 140 150
AGAKIGSYAQ KAWESAPVQL SKKVISSTAN TVATGVDTAT KPVRETAFYK
160 170 180 190 200
TIKQTMSDGS TSSRYGFYAD KEQRKKLREE FERRNRMFAS SARIQPNEDV
210 220 230 240 250
QSVVVHSNPS WKNKVEQIKN ESRLVRKIQE LKKSYQESEH PIVSSIRDMA
260 270 280 290 300
DSISGVWSRM FSETEASQVM RRFKEIDPSF NTEHFLQYLR EYIVPEVTEA
310 320 330 340 350
YVKGDKEVLK TWLSEAPFSV YETTTKEYAK HGVVSVGKIL DIRGVDIMSQ
360 370 380 390 400
RLLQPNDIPV FIVTFRTQEV HMFKDASSGE LVAGKDDRIQ QCTYASVFTR
410 420
VEDELDNPET RGWRIVDFAR ARAVDYF
Length:427
Mass (Da):48,550
Last modified:August 1, 1998 - v1
Checksum:i22F3F1C61FEB8E47
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA18420.1
PIRiT39430
RefSeqiNP_595905.1, NM_001021813.2

Genome annotation databases

EnsemblFungiiSPBC14C8.02.1; SPBC14C8.02.1:pep; SPBC14C8.02
GeneIDi2539636
KEGGispo:SPBC14C8.02

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA18420.1
PIRiT39430
RefSeqiNP_595905.1, NM_001021813.2

3D structure databases

ProteinModelPortaliO60084
SMRiO60084
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO60084, 2 interactors
STRINGi4896.SPBC14C8.02.1

Proteomic databases

MaxQBiO60084
PaxDbiO60084
PRIDEiO60084

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC14C8.02.1; SPBC14C8.02.1:pep; SPBC14C8.02
GeneIDi2539636
KEGGispo:SPBC14C8.02

Organism-specific databases

EuPathDBiFungiDB:SPBC14C8.02
PomBaseiSPBC14C8.02 tim44

Phylogenomic databases

HOGENOMiHOG000208172
InParanoidiO60084
KOiK17804
OMAiRFLKQCE
OrthoDBiEOG092C2GAN
PhylomeDBiO60084

Miscellaneous databases

PROiPR:O60084

Family and domain databases

InterProiView protein in InterPro
IPR032710 NTF2-like_dom_sf
IPR017303 Tim44
IPR039544 Tim44-like
IPR007379 Tim44-like_dom
PANTHERiPTHR10721 PTHR10721, 1 hit
PfamiView protein in Pfam
PF04280 Tim44, 1 hit
PIRSFiPIRSF037871 TIM44, 1 hit
SMARTiView protein in SMART
SM00978 Tim44, 1 hit
SUPFAMiSSF54427 SSF54427, 1 hit
TIGRFAMsiTIGR00984 3a0801s03tim44, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTIM44_SCHPO
AccessioniPrimary (citable) accession number: O60084
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: November 7, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
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Main funding by: National Institutes of Health

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