UniProtKB - O59858 (GPX1_SCHPO)
Protein
Glutathione peroxidase-like peroxiredoxin gpx1
Gene
gpx1
Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Functioni
Glutathione peroxidase-like protein that protects cells during oxidative stress. Has peroxidase activity reducing hydrogen peroxide, alkyl and phospholipid hydroperoxides using preferentially thioredoxin as a reducing power. May act as a scavenger of H2O2.2 Publications
Miscellaneous
The active site is a conserved redox-active cysteine residue, the peroxidatic cysteine (C(P)), which makes the nucleophilic attack on the peroxide substrate. The peroxide oxidizes the C(P)-SH to cysteine sulfenic acid (C(P)-SOH), which then reacts with another cysteine residue, the resolving cysteine (C(R)), to form a disulfide bridge. The disulfide is subsequently reduced by an appropriate electron donor to complete the catalytic cycle. In this atypical 2-Cys peroxiredoxin, C(R) is present in the same subunit to form an intramolecular disulfide.By similarity
Caution
Was originally thought to be a glutathione peroxidase (PubMed:10455235), but functions as an atypical 2-Cys peroxiredoxin using thioredoxin as reducing power instead (PubMed:18162174).2 Publications
Catalytic activityi
- EC:1.11.1.241 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 36 | Cysteine sulfenic acid (-SOH) intermediateBy similarity | 1 |
GO - Molecular functioni
- peroxidase activity Source: GO_Central
- thioredoxin peroxidase activity Source: PomBase
GO - Biological processi
- cell redox homeostasis Source: PomBase
- cellular detoxification of hydrogen peroxide Source: PomBase
- hydrogen peroxide catabolic process Source: PomBase
Keywordsi
Molecular function | Antioxidant, Oxidoreductase, Peroxidase |
Biological process | Stress response |
Enzyme and pathway databases
Reactomei | R-SPO-2142712, Synthesis of 12-eicosatetraenoic acid derivatives R-SPO-9018676, Biosynthesis of D-series resolvins R-SPO-9018896, Biosynthesis of E-series 18(S)-resolvins R-SPO-9020265, Biosynthesis of aspirin-triggered D-series resolvins R-SPO-9023661, Biosynthesis of E-series 18(R)-resolvins |
Protein family/group databases
PeroxiBasei | 3744, SpomGPx01 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutathione peroxidase-like peroxiredoxin gpx1Curated (EC:1.11.1.241 Publication)Alternative name(s): Glutathione peroxidase homolog1 Publication Short name: GPx Thioredoxin peroxidase gpx11 Publication |
Gene namesi | Name:gpx11 Publication ORF Names:SPBC32F12.03cImported |
Organismi | Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) |
Taxonomic identifieri | 284812 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces › |
Proteomesi |
|
Organism-specific databases
PomBasei | SPBC32F12.03c, gpx1 |
VEuPathDBi | FungiDB:SPBC32F12.03c |
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Mitochondrion
- Mitochondrion 1 Publication
Cytosol
- cytosol Source: PomBase
Mitochondrion
- mitochondrion Source: PomBase
Nucleus
- nucleus Source: PomBase
Other locations
- cytoplasm Source: PomBase
Keywords - Cellular componenti
Cytoplasm, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000066644 | 1 – 158 | Glutathione peroxidase-like peroxiredoxin gpx1Add BLAST | 158 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 36 ↔ 82 | Redox-activeBy similarity |
Keywords - PTMi
Disulfide bondProteomic databases
MaxQBi | O59858 |
PaxDbi | O59858 |
PRIDEi | O59858 |
PTM databases
iPTMneti | O59858 |
Expressioni
Inductioni
By oxidative, osmo- and heat stress.1 Publication
Interactioni
Subunit structurei
Monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 276755, 15 interactors |
STRINGi | 4896.SPBC32F12.03c.1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the glutathione peroxidase family.Curated
Keywords - Domaini
Redox-active centerPhylogenomic databases
eggNOGi | KOG1651, Eukaryota |
HOGENOMi | CLU_029507_3_2_1 |
InParanoidi | O59858 |
OMAi | IGFPANN |
PhylomeDBi | O59858 |
Family and domain databases
CDDi | cd00340, GSH_Peroxidase, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000889, Glutathione_peroxidase IPR029759, GPX_AS IPR029760, GPX_CS IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR11592, PTHR11592, 1 hit |
Pfami | View protein in Pfam PF00255, GSHPx, 1 hit |
PIRSFi | PIRSF000303, Glutathion_perox, 1 hit |
PRINTSi | PR01011, GLUTPROXDASE |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS00460, GLUTATHIONE_PEROXID_1, 1 hit PS00763, GLUTATHIONE_PEROXID_2, 1 hit PS51355, GLUTATHIONE_PEROXID_3, 1 hit |
i Sequence
Sequence statusi: Complete.
O59858-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSHFYDLAPK DKDGNPFPFS NLKGKVVLVV NTASKCGFTP QYKGLEALYQ
60 70 80 90 100
KYKDRGFIIL GFPCNQFGNQ EPGSDEEIAQ FCQKNYGVTF PVLAKINVNG
110 120 130 140 150
DNVDPVYQFL KSQKKQLGLE RIKWNFEKFL VNRQGQVIER YSSISKPEHL
ENDIESVL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB012395 Genomic DNA Translation: BAA25326.1 CU329671 Genomic DNA Translation: CAA19364.1 |
PIRi | T43376 |
RefSeqi | NP_596146.1, NM_001022065.2 |
Genome annotation databases
EnsemblFungii | SPBC32F12.03c.1; SPBC32F12.03c.1:pep; SPBC32F12.03c |
GeneIDi | 2540222 |
KEGGi | spo:SPBC32F12.03c |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB012395 Genomic DNA Translation: BAA25326.1 CU329671 Genomic DNA Translation: CAA19364.1 |
PIRi | T43376 |
RefSeqi | NP_596146.1, NM_001022065.2 |
3D structure databases
SMRi | O59858 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 276755, 15 interactors |
STRINGi | 4896.SPBC32F12.03c.1 |
Protein family/group databases
PeroxiBasei | 3744, SpomGPx01 |
PTM databases
iPTMneti | O59858 |
Proteomic databases
MaxQBi | O59858 |
PaxDbi | O59858 |
PRIDEi | O59858 |
Genome annotation databases
EnsemblFungii | SPBC32F12.03c.1; SPBC32F12.03c.1:pep; SPBC32F12.03c |
GeneIDi | 2540222 |
KEGGi | spo:SPBC32F12.03c |
Organism-specific databases
PomBasei | SPBC32F12.03c, gpx1 |
VEuPathDBi | FungiDB:SPBC32F12.03c |
Phylogenomic databases
eggNOGi | KOG1651, Eukaryota |
HOGENOMi | CLU_029507_3_2_1 |
InParanoidi | O59858 |
OMAi | IGFPANN |
PhylomeDBi | O59858 |
Enzyme and pathway databases
Reactomei | R-SPO-2142712, Synthesis of 12-eicosatetraenoic acid derivatives R-SPO-9018676, Biosynthesis of D-series resolvins R-SPO-9018896, Biosynthesis of E-series 18(S)-resolvins R-SPO-9020265, Biosynthesis of aspirin-triggered D-series resolvins R-SPO-9023661, Biosynthesis of E-series 18(R)-resolvins |
Miscellaneous databases
PROi | PR:O59858 |
Family and domain databases
CDDi | cd00340, GSH_Peroxidase, 1 hit |
Gene3Di | 3.40.30.10, 1 hit |
InterProi | View protein in InterPro IPR000889, Glutathione_peroxidase IPR029759, GPX_AS IPR029760, GPX_CS IPR036249, Thioredoxin-like_sf |
PANTHERi | PTHR11592, PTHR11592, 1 hit |
Pfami | View protein in Pfam PF00255, GSHPx, 1 hit |
PIRSFi | PIRSF000303, Glutathion_perox, 1 hit |
PRINTSi | PR01011, GLUTPROXDASE |
SUPFAMi | SSF52833, SSF52833, 1 hit |
PROSITEi | View protein in PROSITE PS00460, GLUTATHIONE_PEROXID_1, 1 hit PS00763, GLUTATHIONE_PEROXID_2, 1 hit PS51355, GLUTATHIONE_PEROXID_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GPX1_SCHPO | |
Accessioni | O59858Primary (citable) accession number: O59858 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | August 1, 1998 | |
Last modified: | February 10, 2021 | |
This is version 146 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Schizosaccharomyces pombe
Schizosaccharomyces pombe: entries and gene names - SIMILARITY comments
Index of protein domains and families