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Entry version 135 (07 Apr 2021)
Sequence version 2 (01 May 1999)
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Protein

2-isopropylmalate synthase

Gene

leu3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score:

Annotation score:3 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Protein inferred from homologyi <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-leucine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate. This subpathway is part of the pathway L-leucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-leucine from 3-methyl-2-oxobutanoate, the pathway L-leucine biosynthesis and in Amino-acid biosynthesis.

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Enzyme and pathway databases

UniPathway: a resource for the exploration and annotation of metabolic pathways

More...
UniPathwayi
UPA00048;UER00070

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
2-isopropylmalate synthase (EC:2.3.3.13)
Alternative name(s):
Alpha-IPM synthase
Alpha-isopropylmalate synthase
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:leu3
ORF Names:SPBC3E7.16c, SPBC4F6.03c
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri284812 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002485 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome II

Organism-specific databases

Schizosaccharomyces pombe database

More...
PomBasei
SPBC3E7.16c, leu3

Eukaryotic Pathogen, Vector and Host Database Resources

More...
VEuPathDBi
FungiDB:SPBC3E7.16c

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001404451 – 5842-isopropylmalate synthaseAdd BLAST584

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
O59736

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
O59736

PRoteomics IDEntifications database

More...
PRIDEi
O59736

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
O59736

SwissPalm database of S-palmitoylation events

More...
SwissPalmi
O59736

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...
BioGRIDi
277518, 8 interactors

STRING: functional protein association networks

More...
STRINGi
4896.SPBC3E7.16c.1

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
O59736

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family_and_domains_section">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini45 – 323Pyruvate carboxyltransferasePROSITE-ProRule annotationAdd BLAST279

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...
eggNOGi
KOG2367, Eukaryota

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
CLU_004588_3_0_1

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
O59736

Identification of Orthologs from Complete Genome Data

More...
OMAi
WPDKVID

Database for complete collections of gene phylogenies

More...
PhylomeDBi
O59736

Family and domain databases

Conserved Domains Database

More...
CDDi
cd07942, DRE_TIM_LeuA, 1 hit

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.20.20.70, 1 hit
3.30.160.270, 1 hit

HAMAP database of protein families

More...
HAMAPi
MF_00572, LeuA_type2, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR013709, 2-isopropylmalate_synth_dimer
IPR002034, AIPM/Hcit_synth_CS
IPR013785, Aldolase_TIM
IPR005668, IPM_Synthase
IPR036230, LeuA_allosteric_dom_sf
IPR039371, LeuA_N_DRE-TIM
IPR000891, PYR_CT

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF00682, HMGL-like, 1 hit
PF08502, LeuA_dimer, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM00917, LeuA_dimer, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF110921, SSF110921, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR00970, leuA_yeast, 1 hit

PROSITE; a protein domain and family database

More...
PROSITEi
View protein in PROSITE
PS00815, AIPM_HOMOCIT_SYNTH_1, 1 hit
PS00816, AIPM_HOMOCIT_SYNTH_2, 1 hit
PS50991, PYR_CT, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

O59736-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKSTFEAAGR VAKGMLKDPS KKYKPFKGIQ LPNRQWPNKV LTKAPRWLST
60 70 80 90 100
DLRDGNQALP DPMNGQEKLR YFKLLCSIGF KEIEVGFPSA SQTDFAFVRH
110 120 130 140 150
LIETPGLIPD DVTISALTPS REPLILRTIE ALRGAKNATV HLYNACSPLF
160 170 180 190 200
REVVFRNSKQ ETLDLAIKGS KIVTAATKNA LESKETNWGF EYSPETFSDT
210 220 230 240 250
EPDFALEVCE AVKGMWKPSA AQPIIFNLPA TVEMSTPNTY ADLIEYFSTN
260 270 280 290 300
ISEREKVCVS LHPHNDRGTA VAAAELGQLA GGDRIEGCLF GNGERTGNVD
310 320 330 340 350
LVTLAFNLYT QGVSPNLDFS KLDEIIRITE DCNKINVHPR HPYAGNLVFT
360 370 380 390 400
AFSGSHQDAI SKGLKAYDER KAVDPVWKVP YLPLDPHDVN SEYAAIIRVN
410 420 430 440 450
SQSGKGGVAY LLKTNCGLDL PRALQVEFGS IVKDYSDTKG KELSIGEISD
460 470 480 490 500
LFYTTYYLEF PGRFSVNDYT LSSNGPQSKC IKCVVDIKGE KKDTPSRVVI
510 520 530 540 550
EGVGNGPLSA LVDALRRQFN ISFDIGQYSE HAIGSGNGVK AASYVEIIFN
560 570 580
NTSFWGVGID ADVTSAGLKA VMSGVSRASR AFAK
Length:584
Mass (Da):64,042
Last modified:May 1, 1999 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i9371A03F82C032F6
GO

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA19019.1

Protein sequence database of the Protein Information Resource

More...
PIRi
T40500

NCBI Reference Sequences

More...
RefSeqi
NP_596103.2, NM_001022019.3

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
SPBC3E7.16c.1; SPBC3E7.16c.1:pep; SPBC3E7.16c

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
2541003

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
spo:SPBC3E7.16c

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329671 Genomic DNA Translation: CAA19019.1
PIRiT40500
RefSeqiNP_596103.2, NM_001022019.3

3D structure databases

SMRiO59736
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi277518, 8 interactors
STRINGi4896.SPBC3E7.16c.1

PTM databases

iPTMnetiO59736
SwissPalmiO59736

Proteomic databases

MaxQBiO59736
PaxDbiO59736
PRIDEiO59736

Genome annotation databases

EnsemblFungiiSPBC3E7.16c.1; SPBC3E7.16c.1:pep; SPBC3E7.16c
GeneIDi2541003
KEGGispo:SPBC3E7.16c

Organism-specific databases

PomBaseiSPBC3E7.16c, leu3
VEuPathDBiFungiDB:SPBC3E7.16c

Phylogenomic databases

eggNOGiKOG2367, Eukaryota
HOGENOMiCLU_004588_3_0_1
InParanoidiO59736
OMAiWPDKVID
PhylomeDBiO59736

Enzyme and pathway databases

UniPathwayiUPA00048;UER00070

Miscellaneous databases

Protein Ontology

More...
PROi
PR:O59736

Family and domain databases

CDDicd07942, DRE_TIM_LeuA, 1 hit
Gene3Di3.20.20.70, 1 hit
3.30.160.270, 1 hit
HAMAPiMF_00572, LeuA_type2, 1 hit
InterProiView protein in InterPro
IPR013709, 2-isopropylmalate_synth_dimer
IPR002034, AIPM/Hcit_synth_CS
IPR013785, Aldolase_TIM
IPR005668, IPM_Synthase
IPR036230, LeuA_allosteric_dom_sf
IPR039371, LeuA_N_DRE-TIM
IPR000891, PYR_CT
PfamiView protein in Pfam
PF00682, HMGL-like, 1 hit
PF08502, LeuA_dimer, 1 hit
SMARTiView protein in SMART
SM00917, LeuA_dimer, 1 hit
SUPFAMiSSF110921, SSF110921, 1 hit
TIGRFAMsiTIGR00970, leuA_yeast, 1 hit
PROSITEiView protein in PROSITE
PS00815, AIPM_HOMOCIT_SYNTH_1, 1 hit
PS00816, AIPM_HOMOCIT_SYNTH_2, 1 hit
PS50991, PYR_CT, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiLEU1_SCHPO
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O59736
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: April 7, 2021
This is version 135 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families
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