UniProtKB - O59413 (DEGLY_PYRHO)
Deglycase PH1704
PH1704
Functioni
Deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins and reactive carbonyl groups of glyoxals. Thus, functions as a protein deglycase that repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage (By similarity).
Also displays proteolytic activity (PubMed:11114201).
By similarity1 PublicationCatalytic activityi
- H2O + Nω-(1-hydroxy-2-oxopropyl)-L-arginyl-[protein] = H+ + L-arginyl-[protein] + lactateBy similarityEC:3.5.1.124By similarity
- EC:3.5.1.124By similarity
- H2O + S-(1-hydroxy-2-oxopropyl)-L-cysteinyl-[protein] = H+ + L-cysteinyl-[protein] + lactateBy similarityEC:3.5.1.124By similarity
- H2O + Nω-(1-hydroxy-2-oxoethyl)-L-arginyl-[protein] = glycolate + H+ + L-arginyl-[protein]By similarityEC:3.5.1.124By similarity
- EC:3.5.1.124By similarity
- H2O + S-(1-hydroxy-2-oxoethyl)-L-cysteinyl-[protein] = glycolate + H+ + L-cysteinyl-[protein]By similarityEC:3.5.1.124By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 100 | Nucleophile1 Publication | 1 | |
Active sitei | 101 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- peptidase activity Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase, Protease |
Enzyme and pathway databases
BRENDAi | 3.4.11.B7, 5244 3.4.22.B78, 5244 |
Protein family/group databases
MEROPSi | C56.001 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Ordered Locus Names:PH1704 |
Organismi | Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) |
Taxonomic identifieri | 70601 [NCBI] |
Taxonomic lineagei | Archaea › Euryarchaeota › Thermococci › Thermococcales › Thermococcaceae › Pyrococcus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000157827 | 1 – 166 | Deglycase PH1704Add BLAST | 166 |
Interactioni
Subunit structurei
Homohexamer formed by a dimer of trimers that assemble into a hollow ring structure.
1 PublicationProtein-protein interaction databases
STRINGi | 70601.3258135 |
Structurei
Secondary structure
3D structure databases
SMRi | O59413 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | O59413 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 166 | PfpI endopeptidasePROSITE-ProRule annotationAdd BLAST | 166 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | arCOG00769, Archaea |
OMAi | MVGHTVH |
OrthoDBi | 98884at2157 |
Family and domain databases
Gene3Di | 3.40.50.880, 1 hit |
InterProi | View protein in InterPro IPR006286, C56_PfpI IPR029062, Class_I_gatase-like IPR002818, DJ-1/PfpI |
PANTHERi | PTHR42733, PTHR42733, 1 hit |
Pfami | View protein in Pfam PF01965, DJ-1_PfpI, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit |
TIGRFAMsi | TIGR01382, PfpI, 1 hit |
PROSITEi | View protein in PROSITE PS51276, PEPTIDASE_C56_PFPI, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MKVLFLTANE FEDVELIYPY HRLKEEGHEV YIASFERGTI TGKHGYSVKV
60 70 80 90 100
DLTFDKVNPE EFDALVLPGG RAPERVRLNE KAVSIARKMF SEGKPVASIC
110 120 130 140 150
HGPQILISAG VLRGRKGTSY PGIKDDMINA GVEWVDAEVV VDGNWVSSRV
160
PADLYAWMRE FVKLLK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000001 Genomic DNA Translation: BAA30818.1 |
PIRi | C71178 |
RefSeqi | WP_010885770.1, NC_000961.1 |
Genome annotation databases
EnsemblBacteriai | BAA30818; BAA30818; BAA30818 |
GeneIDi | 1442551 |
KEGGi | pho:PH1704 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000001 Genomic DNA Translation: BAA30818.1 |
PIRi | C71178 |
RefSeqi | WP_010885770.1, NC_000961.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1G2I | X-ray | 2.00 | A/B/C | 1-166 | [»] | |
6F2F | X-ray | 1.65 | A/B/C | 1-166 | [»] | |
6F2H | X-ray | 2.19 | A/B/C/D/E/F/G/H/I/J/K/L | 1-166 | [»] | |
6HF6 | X-ray | 2.00 | A/B/C | 1-166 | [»] | |
6Q3T | X-ray | 2.15 | A/B/C | 1-166 | [»] | |
SMRi | O59413 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 70601.3258135 |
Protein family/group databases
MEROPSi | C56.001 |
Genome annotation databases
EnsemblBacteriai | BAA30818; BAA30818; BAA30818 |
GeneIDi | 1442551 |
KEGGi | pho:PH1704 |
Phylogenomic databases
eggNOGi | arCOG00769, Archaea |
OMAi | MVGHTVH |
OrthoDBi | 98884at2157 |
Enzyme and pathway databases
BRENDAi | 3.4.11.B7, 5244 3.4.22.B78, 5244 |
Miscellaneous databases
EvolutionaryTracei | O59413 |
Family and domain databases
Gene3Di | 3.40.50.880, 1 hit |
InterProi | View protein in InterPro IPR006286, C56_PfpI IPR029062, Class_I_gatase-like IPR002818, DJ-1/PfpI |
PANTHERi | PTHR42733, PTHR42733, 1 hit |
Pfami | View protein in Pfam PF01965, DJ-1_PfpI, 1 hit |
SUPFAMi | SSF52317, SSF52317, 1 hit |
TIGRFAMsi | TIGR01382, PfpI, 1 hit |
PROSITEi | View protein in PROSITE PS51276, PEPTIDASE_C56_PFPI, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DEGLY_PYRHO | |
Accessioni | O59413Primary (citable) accession number: O59413 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 15, 1999 |
Last sequence update: | August 1, 1998 | |
Last modified: | February 23, 2022 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- Peptidase families
Classification of peptidase families and list of entries - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families