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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35NADUniRule annotation1
Binding sitei62NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei126NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi154Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi169IronUniRule annotation1
Binding sitei217NAD; via amide nitrogenUniRule annotation1
Binding sitei227GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi54 – 55NADUniRule annotation2
Nucleotide bindingi152 – 154NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1324-MONOMER
UniPathwayiUPA00060

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:PH1357
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001539531 – 252Thiamine thiazole synthaseAdd BLAST252

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi70601.PH1357

Structurei

3D structure databases

ProteinModelPortaliO59082
SMRiO59082
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574 Archaea
COG1635 LUCA
HOGENOMiHOG000106048
KOiK03146
OMAiMWGGGMM
OrthoDBiPOG093Z09PU

Family and domain databases

Gene3Di3.50.50.60, 1 hit
HAMAPiMF_00304 Thi4, 1 hit
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR002922 Thi4_fam
IPR022828 Thi4_prok
SUPFAMiSSF51905 SSF51905, 1 hit
TIGRFAMsiTIGR00292 TIGR00292, 1 hit

Sequencei

Sequence statusi: Complete.

O59082-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLREVTITRA IVESYYRDLL NNLELDVAIV GAGPSGMVAA YYLAKGGAKV
60 70 80 90 100
AIFEKKLSIG GGIWGGGMGF NKVVVQDEAR EILDEFGIRY EEFEKGYYVA
110 120 130 140 150
DAIEVATTIA SKVVKSGVKI FNMIEVEDLV IKDNRVSGIV INWTPVLMAG
160 170 180 190 200
LHVDPLTIEA KYVIDSTGHG AQVAQFLVKR GLLKEIPGEG AMWAEQGEKL
210 220 230 240 250
TVKNTREVFP GLYVTGMAAN AIAGAPRMGP IFGGMFLSGR KAAQEILKKL

KS
Length:252
Mass (Da):27,245
Last modified:October 31, 2006 - v2
Checksum:iC92A58226CDBA4C5
GO

Sequence cautioni

The sequence BAA30463 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30463.1 Different initiation.
PIRiG71007
RefSeqiWP_048053375.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30463; BAA30463; BAA30463
GeneIDi1443683
KEGGipho:PH1357

Similar proteinsi

Entry informationi

Entry nameiTHI4_PYRHO
AccessioniPrimary (citable) accession number: O59082
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 31, 2006
Last modified: June 20, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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