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Protein

5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase

Gene

purP

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates.UniRule annotation

Catalytic activityi

ATP + formate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = ADP + phosphate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 1 Mg2+ or Mn2+ ion per subunit.By similarity

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. 5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase (purP)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10SubstrateUniRule annotation1
Binding sitei70SubstrateUniRule annotation1
Binding sitei203ATPUniRule annotation1
Binding sitei231SubstrateUniRule annotation1
Metal bindingi269Magnesium or manganeseUniRule annotation1
Metal bindingi282Magnesium or manganeseUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi121 – 181ATPUniRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1316-MONOMER
UniPathwayi
UPA00074;UER00134

Names & Taxonomyi

Protein namesi
Recommended name:
5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetaseUniRule annotation (EC:6.3.4.23UniRule annotation)
Alternative name(s):
5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligaseUniRule annotation
Gene namesi
Name:purPUniRule annotation
Ordered Locus Names:PH1348
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003486371 – 3335-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetaseAdd BLAST333

Proteomic databases

PRIDEiO59073

Interactioni

Protein-protein interaction databases

STRINGi70601.PH1348

Structurei

3D structure databases

ProteinModelPortaliO59073
SMRiO59073
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini91 – 324ATP-graspUniRule annotationAdd BLAST234

Sequence similaritiesi

Belongs to the phosphohexose mutase family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG04346 Archaea
COG1759 LUCA
HOGENOMiHOG000228474
KOiK06863
OMAiCLEGVFT
OrthoDBiPOG093Z04BP

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_01163 IMP_biosynth_PurP, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR023656 IMP_biosynth_PurP
IPR009720 IMP_biosynth_PurP_C
IPR010672 IMP_biosynth_PurP_N
IPR016185 PreATP-grasp_dom_sf
PANTHERiPTHR38147 PTHR38147, 1 hit
PfamiView protein in Pfam
PF06849 DUF1246, 1 hit
PF06973 DUF1297, 1 hit
PIRSFiPIRSF004602 ATPgrasp_PurP, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit

Sequencei

Sequence statusi: Complete.

O59073-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVRIATYASH SALQILKGAK DEGFGTIAFG KPRVKLLYTK YFPVADYFLE
60 70 80 90 100
GDYPEDKLLE LDAVVIPTGS FIAHLGVELV EKMRVPYFGN KEVLKWESDR
110 120 130 140 150
SLERKWLEKA KLTLPRIYED PDDIDRPVIV KPHGAKGGRG YFIAKSSKDF
160 170 180 190 200
WEKAEKFLGI RSKEDLKNVQ IQEYVVGVPI YPHYFYSKIT GELELMSIDR
210 220 230 240 250
RYESNVDAIG RIPSREQLDL DLDVTYTVVG NIPLVLRESL LMDVIEAGER
260 270 280 290 300
VVKASEELMG GLWGPFCLEG VFTPDMEFVV FEISARIVAG TNLFINGSPY
310 320 330
TWLKYDEPMS TGRRIAREIK IAIEEGKLEE VVT
Length:333
Mass (Da):37,874
Last modified:August 1, 1998 - v1
Checksum:i65792D09C2A540CB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30454.1
PIRiF71006
RefSeqiWP_010885437.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30454; BAA30454; BAA30454
GeneIDi1443674
KEGGipho:PH1348

Similar proteinsi

Entry informationi

Entry nameiPURP_PYRHO
AccessioniPrimary (citable) accession number: O59073
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: August 1, 1998
Last modified: June 20, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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