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Protein

GMP synthase [glutamine-hydrolyzing] subunit B

Gene

guaAB

Organism
Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the synthesis of GMP from XMP.By similarity

Catalytic activityi

ATP + XMP + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.

Pathwayi: GMP biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes GMP from XMP (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. GMP synthase [glutamine-hydrolyzing] subunit A (guaAA), GMP synthase [glutamine-hydrolyzing] subunit B (guaAB)
This subpathway is part of the pathway GMP biosynthesis, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes GMP from XMP (L-Gln route), the pathway GMP biosynthesis and in Purine metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi28 – 34ATPBy similarity7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processGMP biosynthesis, Purine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPHOR70601:G1G39-1315-MONOMER
BRENDAi6.3.5.2 5244
UniPathwayiUPA00189; UER00296

Names & Taxonomyi

Protein namesi
Recommended name:
GMP synthase [glutamine-hydrolyzing] subunit B (EC:6.3.5.2)
Alternative name(s):
GMP synthetase
Gene namesi
Name:guaAB
Ordered Locus Names:PH1347
OrganismiPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
Taxonomic identifieri70601 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000000752 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001402491 – 308GMP synthase [glutamine-hydrolyzing] subunit BAdd BLAST308

Interactioni

Subunit structurei

Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).Curated

Protein-protein interaction databases

STRINGi70601.PH1347

Structurei

Secondary structure

1308
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 18Combined sources16
Beta strandi23 – 26Combined sources4
Helixi31 – 44Combined sources14
Helixi45 – 47Combined sources3
Beta strandi48 – 54Combined sources7
Helixi62 – 70Combined sources9
Turni71 – 74Combined sources4
Beta strandi77 – 82Combined sources6
Helixi84 – 90Combined sources7
Turni91 – 93Combined sources3
Helixi97 – 119Combined sources23
Beta strandi122 – 125Combined sources4
Beta strandi156 – 158Combined sources3
Turni160 – 163Combined sources4
Helixi166 – 175Combined sources10
Helixi180 – 183Combined sources4
Helixi192 – 196Combined sources5
Beta strandi197 – 200Combined sources4
Helixi203 – 222Combined sources20
Beta strandi228 – 234Combined sources7
Beta strandi239 – 259Combined sources21
Beta strandi261 – 268Combined sources8
Helixi273 – 286Combined sources14
Beta strandi290 – 296Combined sources7

3D structure databases

ProteinModelPortaliO59072
SMRiO59072
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO59072

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 185GMPS ATP-PPaseAdd BLAST185

Phylogenomic databases

eggNOGiarCOG00085 Archaea
COG0519 LUCA
HOGENOMiHOG000223964
KOiK01951
OMAiEGGIKSH
OrthoDBiPOG093Z04V2

Family and domain databases

CDDicd01997 GMP_synthase_C, 1 hit
Gene3Di3.40.50.620, 1 hit
HAMAPiMF_00345 GMP_synthase_B, 1 hit
InterProiView protein in InterPro
IPR001674 GMP_synth_C
IPR026598 GMP_synthase_B
IPR025777 GMPS_ATP_PPase_dom
IPR022310 NAD/GMP_synthase
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00958 GMP_synt_C, 1 hit
PF02540 NAD_synthase, 2 hits
TIGRFAMsiTIGR00884 guaA_Cterm, 1 hit
PROSITEiView protein in PROSITE
PS51553 GMPS_ATP_PPASE, 1 hit

Sequencei

Sequence statusi: Complete.

O59072-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDWGRFVEEK VREIRETVGD SKAIIALSGG VDSSTAAVLA HKAIGDRLHA
60 70 80 90 100
VFVNTGFLRK GEPEFVVKTF RDEFGMNLHY VDAQDRFFSA LKGVTDPEEK
110 120 130 140 150
RKIIGRVFIE VFEEVAKKIG AEYLIQGTIA PDWIESQGKI KSHHNVGGLP
160 170 180 190 200
EKLNLKLIEP LRDLYKDEVR ELAKFLGLPE KIYNRMPFPG PGLAVRVIGE
210 220 230 240 250
VTPEKIRIVR EANAIVEEEV ERAGLRPWQA FAVLLGVKTV GVQGDIRAYK
260 270 280 290 300
ETIAVRIVES IDGMTANAMN VPWEVLQRIA FRITSEIPEV GRVLYDITNK

PPATIEFE
Length:308
Mass (Da):34,562
Last modified:August 1, 1998 - v1
Checksum:i8B209770605AE4BA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000001 Genomic DNA Translation: BAA30453.1
PIRiE71006
RefSeqiWP_010885436.1, NC_000961.1

Genome annotation databases

EnsemblBacteriaiBAA30453; BAA30453; BAA30453
GeneIDi1443673
KEGGipho:PH1347

Similar proteinsi

Entry informationi

Entry nameiGUAAB_PYRHO
AccessioniPrimary (citable) accession number: O59072
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 1, 1998
Last modified: June 20, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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